• Title/Summary/Keyword: 모세관

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Separation of Bacteria Using Capillary Electrophoresis (모세관 전기영동을 이용한 박테리아의 분리)

  • Moon, Byoung-Geoun;Choi, Kyu-Seong;Lee, Sang-Chun;Kim, Yong-Seong
    • Journal of the Korean Chemical Society
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    • v.48 no.2
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    • pp.144-150
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    • 2004
  • Various experimental factors that affect the separation of bacteria were investigated using capillary electrophoresis. At different buffer concentrations, gram-positive bacteria and gram-negative bacteria showed somewhat different migration behavior under high electric filed. The separation efficiency was also investigated as a function of concentration of bacterium injected into the capillary. In order to separate bacteria as the difference of size and shape, water soluble polymers such as poly(ethylene)oxide (PEO), polyvinylpyirrolidone (PVP), and dextran were studied. PEO, which is more flexible and has lower steric hinderance, showed the best separation efficiency. The mixed bacteria sample of Micrococcus lysodeikticus as gram-positive bacteria and Aerobacter aerogenes as gram-negative bacteria were successfully analyzed with PEO.

Modeling of Capillary Filling Length in Silwet L-77 Added Poly(Dimethylsiloxane) (PDMS) Microchannels (Silwet L-77 이 포함된 Polydimethylsiloxane(PDMS) 마이크로 채널의 유동 길이 모델링)

  • Lee, Bom-Yee;Lee, Bong-Kee
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.38 no.8
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    • pp.823-829
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    • 2014
  • In the present study, simple models were proposed to predict the capillary-driven flow length in a surfactant-added poly(dimethylsiloxane) (PDMS) rectangular microchannel. Owing to the hydrophobic nature of PDMS, it is difficult to transport water in a conventional PDMS microchannel by means of the capillary force alone. To overcome this problem, microchannels with a hydrophilic surface were fabricated using surfactant-added PDMS. By measuring the contact angle change on the surfactant-added PDMS surface, the behavior was investigated to establish a simple model. In order to predict the filling length induced by the capillary force, the Washburn equation was modified in the present study. From the investigation, it was found that the initial rate-of-change of the contact angle affected the filling length. Simple models were developed for three representative cases, and these can be useful tools in designing microfluidic manufacturing techniques including MIcroMolding In Capillaries (MIMIC).

Computer Simulation of Viscoelastic Flow in a Capillary Die for Rubber Compounds (모세관 다이에서 고무 복합체의 점탄성 거동에 대한 컴퓨터 모사)

  • Park, Dong-Myung;Kim, Hok-Joo;Yoon, Jae-Ryong;Lyu, Min-Young
    • Elastomers and Composites
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    • v.41 no.4
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    • pp.223-230
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    • 2006
  • Rubber compounds have a high viscoelastic property. One of the viscoelastic behaviors during profile extrusion is the swelling of extrudate, and the amount of swelling varies with operational conditions in extrusion. It is well recognized that the elastic portion in the viscoelastic property plays an important role in the extrudate swell. In this study computer simulation of the die swell at the capillary die for several rubber compounds has been performed using commercial CFD code, Polyflow. A non-linear differential viscoelastic model, Phan-Thien-Tanner (PTT) model, was used in the computer simulation. Non-isothermal behavior was considered in the calculation. Distribution of pressure, velocity and temperature in the reservoir and capillary die, and extrudate profiles were predicted through the simulation. The amount of the die swell fur the different rubber compounds was investigated for various flow rates and three types of length to diameter of the capillary die. It is concluded that the PTT model successfully represented viscoelastic behavior of rubber compounds.

Comparison of the Heat transport Limitations for Screen Mesh Wick and Sintered Metal Wick Heat Pipes by Theoretical Analysis (이론적 해석에 의한 스크린 메쉬윅과 소결윅 히트파이프의 열수송 한계 비교)

  • Kim Keun-Bae;Kim Yoo
    • Journal of Energy Engineering
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    • v.13 no.4
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    • pp.267-274
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    • 2004
  • Theoretical analysis for predicting the heat transport limitations of screen mesh wick and sintered wick heat pipes was performed. The heat pipe diameter was 8mm and water was used for working fluid. For the 250 mesh, each capillary pressures and heat transport limitations, thermal resistances were analyzed according to the operating temperatures, wick thicknesses and inclination angles, based on the effective capillary radius (r$\_$c/), porosity ($\varepsilon$) and permeability (K). The wick capillary limitation was increased as the operating temperature and the wick thickness were increased, and generally the sintered wick showed higher heat transport limitations than that of the screen wick. The thermal resistance of the screen wick was higher than that of the sintered wick and both thermal resistances were linearly increased as the wick thickness was increased.

Development of a Multi - channel Detector for Capillary Electrophoresis System (모세관 전기영동 장치용 Photodiode Array 다채널 검출기의 개발에 관한 연구)

  • Hong, Seung Guk;Kim, Hai-Dong
    • Analytical Science and Technology
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    • v.11 no.2
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    • pp.96-104
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    • 1998
  • A photodiode array multichannel detector system for capillary electrophoresis was developed. The photodiode array detector for capillary electrophoresis (CE-PDA) has 1024 photodetectors and can analyze sample by measuring UV/VIS absorption spectrum in 275~675 nm wavelength range. The CE-PDA instrument can get a spectrum in 30 ms during sample separation and can be programmed by a PC to control various experimental conditions required for sample analysis. The performance of the multichannel CE-PDA instrument was tested using L-ascorbic acid and alizarin yellow GG mixture. The reproducibility test of the CE-PDA system showed 5.6% RSD.

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Development of Conductivity Cell and Suppressor for Capillary Column Ion Chromatography (모세관 컬럼 이온 크로마토그래피를 위한 Conductivity Cell과 Suppressor의 개발)

  • Pyo, Dongjin;Kim, Hohyun
    • Analytical Science and Technology
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    • v.12 no.2
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    • pp.89-93
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    • 1999
  • In this study, conductivity cell and suppressor for micro-column ion chromatography were developed to analyze ions in small columns of samples. With a capillary column, the flow rate of the mobile phase is so small (usually $5{\sim}20{\mu}L/min$) that the usual conductivity cell can not be used. Therefore, we developed a new type of conductivity cell and suppressor which have small inner volumes. The conductivity cell was made with two Pt hypodermic needles (i.d. 0.010 mm) which are slightly separated (about $2{\mu}m$), and the suppressor was made of Nafion tubings. When several anions(fluoride, nitrite, nitrate, chlorate) were analyzed using developed conductivity cell and suppressor, a good chromatogram was obtained.

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Analysis of Unsaturated Flow Considering Hysteresis in Porous Media under Antecedent Rainfall (선행강우가 존재하는 다공성 매질에서 이력현상을 고려한 비포화 흐름 해석)

  • Park, Chang Kun;Sonu, Jung Ho
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.14 no.5
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    • pp.1137-1143
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    • 1994
  • Through the numerical analysis of the capillary pressure-based Richards equation with and without the effect of the capillary hysteresis under the boundary condition having an antecedent rainfall. the moving tendency of the wetting front, the redistribution of the moisture content, infiltration rate, cumulative infiltration etc, were computed. The effect of the capillary hysteresis cannot be neglected in analyzing an unsaturated flow, and the more accurate results may be obtained by the consideration of the hysteresis effect. If the effect of the hysteresis cannot be considered, the analysis by the use of the main wetting curve may give more reliable result than that of the main drying curve.

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A Study on the Separation and Determination of Organochlorine Pesticides by Capillary Column Gas-liquid Chromatography (모세관 컬럼 기체크로마토그래피에 의한 유기염소제 농약의 분리 정량에 관한 연구)

  • Taek-Jae Kim;Yun-Woo Eo;Young-Sang Kim
    • Journal of the Korean Chemical Society
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    • v.31 no.5
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    • pp.425-433
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    • 1987
  • The separation of 19 organochlorine pesticides was studied by capillary column gas chromatography. The applicability of this method to the fresh rice was investigated. Comparison of the resolution of pesticides by OV-17 and SE-30 capillary columns revealed that OV-17 column (25m ${\times}$ 0.20mm, 0.27${\mu}$m thickness) showed better resolution. The relative standard deviations (RSD) for their retention times and peak areas obtained by splitless injection mode were within 0.16% and 8.2%, respectively. The recoveries of pesticides spiked in fresh rice were mostly over 85% and their RSDS did not exceed 11.4%.

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Simulation of the small Refrigeration Cycle as shape change of the Non-Adiabatic Capillary Tube (비단열 모세관의 형상 변경에 따른 소형 냉동 사이클 성능 해석)

  • Nam, Ki-Won;Yi, Dae-Yong;Park, Sang-Goo;Jeong, Ji-Hawn;Kim, Lyun-Su
    • Proceedings of the SAREK Conference
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    • 2009.06a
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    • pp.550-555
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    • 2009
  • The present simulation focuses on the effect of the shape of capillary tube-suction line heat exchangers(SLHX), The results in the three cases of the SLHX types show that both of the location and length of heat exchange section influence the coefficient of performance(COP) and cooling capacity. Simulation shows the COP may be improved by 4.6% and the cooling capacity may be improved by 13.6% in the Lateral type.

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Studies on Analysis of Sterols in Mayonnaise by GLC with Packed and Capillary Column (충전컬럼 및 모세관컬럼 가스크로마토그라프에 의한 마요네즈 중의 스테롤 분석연구)

  • Kim, Hyean-Wee;Jeong, So-Young;Jeong, Chang-Ki;Yoon, Hyeong-Sik;Park, Ki-Moon;Ahn, Peong-Ug;Choi, Chun-Un
    • Korean Journal of Food Science and Technology
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    • v.24 no.1
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    • pp.82-85
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    • 1992
  • Sterols in mayonnaise were determined by the gas liquid chromatographic(GLC) analysis using two different columns, packed column(silicone SE30) and fused silica capillary column(CBPl). Four kinds of sterol(cholesterol, campesterol, stigmasterol and ${\beta}-sitosterol$) were detected in mayonnaise. The method using capillary column proved to be superior in rapidity, reproducerbility and separability for the determination of sterols. The ranges of concentration of egg york in mayonnaise predicted from cholesterol content using packed and capillary columns were $2.69{\sim}7.11%\;and\;2.42{\sim}6.08%$, respectively. Analyzing the composition of campesterol, stigmasterol and (${\beta}-sitosterol$ in mayonnaise, it could be known that soybean oil and cottonseed oil are commonly used for the manufacture of mayonnaise.

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