• Journal of Life Science
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• v.27 no.4
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• pp.423-429
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• 2017

Hair color is determined by kind and amount of melanin. Melanocyte mainly synthesizes melanin from L-tyrosine by stimulation of ultra violet. Reactive oxygen species (ROS) play an important role in greying hair. Polygonum multiflorum radix has been reported to inhibit the aging process that black color of hair is turned into grey color. The aim of this study is to investigate the effect of Polygoni multiflorium radix ethanol extract (PMEE) on melanin synthesis related to black hair growth. In anti-oxidant experiment, PMEE decreased DPPH radical and increased reducing power, indicating that PMEE could eliminate ROS involved in greying hair. PMEE decreased cell viability in a dose-dependent manner. Furthermore, the effect of PMEE on the production of melanin was determined by DOPA assay and tyrosinase activity. PMEE increased tyrosinase activity and promoted melanin synthesis. In addition, the expression levels of tyrosinase, tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2) and microphthalmia-associated transcription factor (MITF), as well as anti-oxidant enzymes such as superoxide dismutase (SOD-3) and catalase were examined using western blot analysis. The expression levels of SOD-3 and catalase were decreased due to the enhanced antioxidant activity of PMEE. In particular, PMEE increased the expression levels of tyrosinase and TRP-2. These results suggest that PMEE could promote melanin synthesis that involved in tuning gray hair into black hair.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.3
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• pp.1139-1145
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• 2010

This thesis tried applying Ayurvedic hair and scalp care program and analyzing its effect. Ayurvedic treatment strengthens our immunity through natural healing power. I measured with MIC test and Halo test in order to examine the antibacterial efficacy against colonies which reside in head, when Ayurvedic base oils and essential oils were used on hair and scalp. Finally, I reached the results as follows. I examined the antibacterial efficacy of base oils and essential oils against Bacillus subtilis and Propionibacterium acnes, based on the MIC test with 9 kinds of base oil and 18 kinds of essential oil. By way of experiment, base oils did not show the antibacterial efficacy from all colonies, and in case of essential oils, such as Chamomile, Clary sage, Jasmine, Neroli and Rose oil, the antibacterial efficacy against two colonies did not appear by the measure of below 5%. It turned out that Thyme, Geranium, Lavender and Tea tree had an high effect on two colonies. According to the Halo test which experimented on 7 kinds of bacteria with the essential oils like Geranium, Lavender and Thyme and with the base oils like Sesame and Coconut, it went to prove that base oils was inefficient on antibiosis. It was examined that all of essential oils had the effect on antibiosis from 7 kinds of bacteria. Thyme showed the best efficacy of antibiosis in the MIC and the Halo test.

• Journal of Life Science
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• v.29 no.4
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• pp.455-460
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• 2019

In previous studies, we confirmed the effective antimicrobial activity of fermented Dendropanax morbifera leaf/branch extracts with Lactobacillus plantarum ilchiwhangchil 1785 and Lactobacillus plantarum ilchiwhangchil 2020. In this study, we investigated the hair growth effect of D. morbifera leaf/branch extracts fermented with L. plantarum ilchiwhangchil 1785 and L. plantarum ilchiwhangchil 2020 on human hair dermal papilla cells. The growth rate of human hair dermal papilla cells treated with fermented extracts in the range of 1 to $10{\mu}g/ml$ significantly increased in a concentration-dependent manner, without increasing cell death. Double staining studies showed that the growth of cells treated with fermented D. morbifera leaf/branch extracts was more active than that of control cells. Moreover, the cells treated with the fermented D. morbifera leaf/branch extracts exhibited a 18.84% and 23.31% increase in cell mobility, respectively, as compared with that of the untreated cells. High-performance liquid chromatography (HPLC) was used to determine the active agents responsible for hair growth. The results showed that the content of ${\beta}$-sitosterol, which is known to affect hair growth, increased about 10 times in the fermentation process of D. morbifera leaf/branch extracts. Taken together, the findings confirm that fermented Dendropanax morbifera leaf/branch extracts promote hair growth.

• The Journal of Pediatrics of Korean Medicine
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• v.21 no.2
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• pp.51-67
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• 2007

Objectives The study was designed to find out the relationships between short stature and low weight among children and teenagers. Methods This research was performed in 21 children and teenagers from August 2006 to June 2007. We measured height, weight, body mass index, obesity rare, bone age, and HTMA. Moreover, we analyzed correlation with height, weight, mineral concentrations and ratios obtained from HTMA. Results There were significant correlations between HTMA and short stature and low weight. 1. Most subjects(76%) had slow metabolic types. 2. Ca, Mg, Zn, Ca/P, and Cr had significant differences between high bone age group and high chronological age group. 3. Fe/Cu had significant differences between a group that recent height is more smaller than inherited height and a group that recent height is more taller than inherited height. 4. Ca, Mg, Ca/P had significant differences between a low weight group and a normal weight group classified by obesity rate. Conclusions Children and teenagers with short stature and low weight have specific mineral characters.

• Journal of Environmental Health Sciences
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• v.28 no.3
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• pp.64-71
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• 2002

Hair provides important information about the body's mineral status. Therefore, we have studied the trace elemental distribution in human hair under different inhalation and ingestion environment using neutron activation analysis (NAA). NAA is a powerful analytical method which can be used successfully to determine trace elements in environmental and biological materials. Total diet and six different herbal medicine were analysed to study ingestion environment. Airborne dust in foundry was analysed and compared with outdoor dust to study inhalation environment. Human hairs of common person, herbal medicine taker and foundry worker were analysed to estimate the trace elemental distribution of people under different inhalation and ingestion environment. Analytical results show that herbal medicines contain higher micronutrients such as Ca, Mg, Mn and Cu that total diet and airborne dust in foundry has high Cr. The concentration of Ca, Mg and Mn in hair of herbal medicine taker is two times higher than in hair of common person and Cr con-centration in hair of foundry worker is about three times higher than in hair of common person. These results show that NAA can be used importantly to monitor human health through biological and environmental samples.

• YAKHAK HOEJI
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• v.51 no.3
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• pp.206-213
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• 2007

Recently estimating the uncertainty of an analytical result has become an essential part of quantitative analysis. This study describes the uncertainty of the measurement for the determination of methamphetamine and its major metabolite amphetamine in human hair, The method consists of washing, drying, weighing, incubation and extraction with methanolic HCI solution, clean-up, trifluoroacetyl derivatization, and qualification/quantification of residues by gas chromatography/mass spectrometry (GC/MS). Traceability of measurement was established through traceable standards and calibrated volumetric equipment and measuring instruments. Measurement uncertainty associated with each analyte in real samples was estimated using quality control (QC) data. The main source of combined standard uncertainty comprised two components, which are uncertainties associated with calibration linearity and variations in QC, while those associated with preparation of analytical standards and sample weighing were not so important considering the degree of contribution. Relative combined standard uncertainties associated with the described method ranged for individual analytes from 4.99 to5.03%.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.50 no.1
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• pp.37-48
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• 2024

Songla (Usnea diffracta Vain.) is one of the lichens belonging to the genus Usnea, and pharmacological activities such as antioxidant, antimicrobial, anti-inflammatory, anti-tumor and cardiovascular protection have been reported in previous studies, but its efficacy in skin and hair is not well known. In this study, the effect of Usnea diffracta extract (UDE) on anti-aging and dermal papilla cell proliferation was verified in vitro. As a result of the experiment, it was confirmed that the UDE significantly reduced the expression of MMP-1 and the activity of MAPKs (ERK, p38, JNK) and AP-1 (c-Fos, c-Jun), which were increased by UVA in HDFn. In addition, the UDE significantly increased the proliferation of HFDPC and significantly increased the mRNA expression of VEGF and KGF, which are hair growth factors. Accordingly, the phosphorylation of ERK/CREB involved in hair proliferation and expression of growth factors was increased in a concentration-dependent manner. The main component represented by the main peak was separated and purified using Prep LC by concentrating the UDE, which was confirmed as diffractaic acid through NMR and Mess analysis. Isolated diffractaic acid significantly reduced the expression of MMP-1 increased by UVA in HDFn and increased the proliferation of HFDPC in a concentration-dependent manner. The result suggest that UDE proved its usability as a natural cosmetic material with anti-aging and dermal papilla cell activation effects.

• Journal of Convergence for Information Technology
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• v.10 no.4
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• pp.175-183
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• 2020

The change in the demand and perception of wigs worn for hair loss is getting better, but hair extension is emerging as a new treatment method that improves as there are inconvenient areas such as hair loss, bleaching, and tangling of wigs themselves, and time spent on unnatural hair styling and wearing wigs. The purpose of this study is to verify that the quality of life of middle-aged female hair loss is positively affected by hair growth techniques that weave wool strands into their hair. Those surveyed were middle-aged women with hair loss and were analyzed by measuring the quality of life before and after hair growth. The analysis showed that hair increased techniques affects the economic satisfaction, physical satisfaction, mental satisfaction and social satisfaction of middle-aged women with hair loss, indicating that hair increased techniques is effective in improving the quality of life for middle-aged women with hair loss. It is also expected that the beauty industry will develop high value through hair increased techniques, a blue ocean in the beauty market.

• YAKHAK HOEJI
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• v.48 no.3
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• pp.207-212
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• 2004

An analytic method was developed for the quantitation of $\Delta$$^{9}-$ tetrahydrocannabinol (THC) and 11-nor-9-carboxy THC (THC-COOH) in human hair. After hair samples were pulverized using Freezer Mill, deuterated internal standards were added and digested in 1 N NaOH at $100^{\circ}C$ water bath for 30 min. Digest solutions were extracted by 5 ml hexane：ethyl acetate (90：10) after acidification with acetic acid. The organic phase was evaporated under N 2 and derivatized by BSTFA (with 1% TMCS) at $85^{\circ}C$ for 45 min. The derivatized solution was separated on HP-5MS column ($30m{\times}0.25mm{\times}0.25mm$) and detected using EI-GC-MS with selective ion monitoring mode. The assay of calibration was ranged from 5 to 100 ng/50 mg hair ($r^2$＞0.99) for THC and THC-COOH. Within and between-run precision were calculated at 6, 30, 60 ng/50 mg hair with coefficients of variation less than 11%. Within and between run accuracies at the same concentrations were$\pm$14% and $\pm$30% of target for both analytes, respectively. Absolute and relative recovery at 10 and 100 ng were 60∼91％. The method was used to detect and quantify THC and THC-COOH in cannabis abuser's hairs (N = 16) and SRM (N=5, THC 1 ng/mg, NIST). We detected THC and THC-COOH in only one hair sample. In SRM, % accuracy was 93% (range 86∼103%) and precision (% CV) was 8.14. We began to set up a quantitative analysis of THC and THC-COOH using EI-GC-MS. Continuously, we need to modify and develop this method in order to apply for identification in cannanbis users' hair.

• Journal of Industrial Convergence
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• v.20 no.4
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• pp.71-77
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• 2022

It is a reality that office workers in modern society cannot overlook their appearance management for promotion in the workplace that does not fall behind in competition with others and smooth human relationships. According to this social trend, it is time to conduct an in-depth cosmetology study on the appearance management and perception of office workers, including hair styles. Therefore, this study analyzed the hair style type and preference closely by cross-analyzing the differences between groups by classifying the occupational persons of female airline crew, female soldiers, female sportsmen, female lawyers, and female nurses into age, marriage, academic background, and occupation. As result of study, it was found that different women in each occupational group had different results, such as their current hair style type and hair texture, the use of current hair styling products, and their personal preference for women's hairstyles and hair colors. This study will be used as basic data for developing new mode items that meet customer needs in the beauty marketing market and researching customer demand.