• Korean Journal of Environmental Agriculture
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• v.35 no.3
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• pp.175-183
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• 2016

BACKGROUND: There's a long rainy season during the Summer in Northeast Asia, including Korea. Heavy rainfall during this season causes harm to tree's root, and damped injury in the pear has been continuously reported. Pear Research Institute is breeding damp resistant rootstocks and investigating their mechanisms to relieve damped damages in the pear.METHODS AND RESULTS: Seedlings of Pyrus betulaefolia and P. calleryana were divided into two groups: control and damped, respectively. Damped group was treated by constant irrigation for 77 days and control group was maintained to keep the soil moisture pressure between 0 and -10 kPa. After the treatment, we analysed trees' growth rate, chlorophyll content, amino acids and total phenolic compounds. As a result, P. betulaefolia was sensitive to damped treatment while P. calleryana did not have significant differences between the control and damped treatment. It was observed that total contents for phenolic compounds were dramatically increased in P. betulaefolia while trees' growth rate, chlorophyll b and general amino acid contents were lowered by damping treatment.CONCLUSION: In some pear cultivars, growth habit is suppressed by damped damage. Pyrus calleryana displayed tolerances to damped damage in growth rate and some organic compound contents compared to P. betulaefolia. So we recommend to exploit P. calleryana as a pear rootstock rather than using P. betulaefolia.

• Sleep Medicine and Psychophysiology
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• v.8 no.1
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• pp.45-51
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• 2001

Objectives: The experience of traffic accident is a kind of the psychosocial stressors to person. The traffic accident-related patients may show the psychophysiologic hyperarousal. So we examined the differences of psychophysiologic response between patients with and without the memory of experienceing a traffic accident. Methods: Twenty-four traffic accident-related patients were divided into two groups according to ther memory of a traffic accident. In psychological assessment, levels of anxiety and depression were evaluated by State-Trait Anxiety Inventory, Beck's Depression Inventory, and Hamilton Rating Scales For Anxiety and Depression. Heart rate, electrodermal response (EDR), and electromyographic activity (EMG) were measured by biofeedback system, and systolic and diastolic blood pressure by automated vital sign monitor during baseline, task, and rest periods. We utilized script-driven imagery technique as a stressful task. The patients listened to the script describing their own traffic accident experience and were instructed to imagine the event during the task period. Statistically analytic data were obtained from the differences of psychological and psychophysiologic data between two groups. Results: The memory group did not show significantly higher EDR than the none memory group, but showed higher tendency during baseline, imagery, and rest periods. The memory group showed significantly lower EMG than the none memory group during rest period. However, there were no differences in other psychophysiologic reponses between the two groups. Conclusion: Our results showed that the memory group had higher tendency in autonomic arousal level such as electrodermal response than the none memory group. We suggest that physicians need to minimize repetitive imagery of traffic accident (reexperience), and decrease the autonomic hyperarousal in the treatment of traffic accident-related patients.

• Applied Microscopy
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• v.35 no.3
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• pp.153-163
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• 2005

This experiment was performed to evaluate the morphological responses of the gastric chief cells of the mouse, inoculated with Ehrlich carcinoma cells in the inguinal area, following administration of BCG (Bacillus Calmette-Guerin). Healthy adult ICR mice weighing 25 gm each were divided into normal and experimental groups (experimental control group and BCG treated group). In the experimental groups, each mouse was inoculated with $1x10^7$ Ehrlich carcinoma cells subcutaneously in the inguinal area. From next day after inoculations, 0.2 mL of saline or BCG (0.5 mL/25 g B.W.: $0.03{\times}10^8{\sim}0.32{\times}10^8$ CFU) were injected subcutaneously to the animals every other day, respectively. The day following the last injection, each mouse was sacrificed. Pieces of the tissue were taken from the stomach, prefixed with 2.5% glutaraldehyde-1.5% paraformaldehyde solution, followed by post-fixation with 1% osmium tetroxide solution. The ultrathin sections were stained with uranyl acetate and lead citrate. The size of zymogen granule and the size of the mitochondrion of the gastric chief cells were observed and calculated. In the BCG treated group, most chief cells did not show any difference in ultrastructure, except that myelin figures were more frequently observed, in comparison with that of nornmal control group. The size of zymogen granule in the gastric chief cells of normal control, experimental control and BCG-treated groups were $0.98({\pm}0.108){\mu}m,\;1.05({\pm}0.092){\mu}m\;and\;0.93({\pm}0.053){\mu}m$, respectively. And the mitochondrial size of the gastric chief cells of normal control, experimental control and BCG-treated groups were $0.80({\pm}0.130){\mu}m,\;0.83({\pm}0.143){\mu}m\;and\;0.72({\pm}0.078){\mu}m$, respectively. From the above results, it was concluded that BCG may slightly suppress function of the gastric chief cells.

• Food Science of Animal Resources
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• v.31 no.6
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• pp.928-934
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• 2011

The objective of this study was to characterize the lactate metabolism and lipolysis in Emmental cheese made of Korean raw milk throughout the ripening periods; 14 d at $10^{\circ}C$, 42 d at $23^{\circ}C$, and 30 d at $4^{\circ}C$. Emmental cheese was made using a commercial starter culture with propionic acid bacteria (PAB) and without PAB as a control on the pilot plant scale. Changes in the contents of five organic acids (citric, lactic, formic, acetic, and propionic acid) and individual free fatty acids (FFAs) were measured using HPLC/PDA and GC/FID. As a result of propionic fermentation by PAB, the concentration of acetic acid and propionic acid increased up to 1.5 and 6.1 g/kg, respectively and the most dramatic increased occurred when incubated in the hot room ($23^{\circ}C$). Lactic, citric, and formic acid contents were 2.6, 2.5 and 0.8 g/kg at the end of ripening, respectively. As a result of lipolysis, the amount of total FFAs was 6,628.2 mg/kg. Compared to the control, levels of individual FFAs from butyric (C6:0) to linoleic (C18:2) acids increased significantly (p<0.05) during the ripening period. Especially, 65.1% of total FFAs was released in the $23^{\circ}C$ room and the most abundant FFAs were palmitic (C16:0), stearic (C18:0) and oleic acid (C18:1). These results demonstrated that the lipolysis of Emmental cheese was strongly affected by bacterial lipase from PAB.

• Journal of the Korea Computer Graphics Society
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• v.11 no.2
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• pp.40-46
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• 2005

본 논문에서는 스윕곡면(sweep surface)을 기반으로 복잡한 3차원 물체의 형상을 변형하는 새로운 자유형상 변형(free-form deformation)기법을 제시한다. 본 논문에서 제시한 방법은 기존의 격자점(control lattices)을 조정하는 체적기반의 방법에 비해 스윕기반의 절차적 (procedural) 모델링 기법의 직관성을 활용하여 사용자에게 보다 효율적인 형상변형 기법을 제공한다. 3차원 물체의 정점들은 스윕곡면의 단면(cross-section)에 바인딩(binding) 되어 곡면의 변화에 따라 형상변형을 한다. 본 논문에서 제시한 스윕기반 형상변형 기법은 3차원 물체를 부분적으로 변형하는 기법과 계층구조를 기반으로 전반적인 형상을 변형하는 기법으로 구분된다. 부분적인 형상변형 기법은 스윕곡면을 구성하는 성분의 다단계(multi-level) 표현을 통하여 사용자에게 다중해상도(multi-resolution) 형상변형 기능을 제공한다. 계층구조에 기반한 형상변형 기법은 복잡한 3차원 물체를 위상구조에 따라 여러 계층으로 분할하고, 각 부분의 형상을 근사하는 스윕곡면들을 생성한다. 분할된 각 부분의 정점(vertices)들은 해당 스윕곡면에 바인딩 되어 스윕곡면의 단면의 변화를 따르게 된다. 이러한 상황하에서 스윕곡면들은 3차원 물체의 계층구조에 따라서 서로 상호작용(interaction)을 하게 되고, 결과적으로 3차원 물체의 각부분은 상호반응적 형상변형을 한다. 이러한 계층구조에 기반한 상호반응적 형상변형 기법은 사용자의 의도에 부합하는 보다 사실적이고 기능적인 형상변형을 가능하게 한다. 본 논문에서는 다양한 3차원 물체에 대한 실험을 통해 제시된 방법의 효율성을 입증한다. 결과 막성 사구체병증과 같은 신질환이 발견될 수 있으므로 신조직검사의 적응증에 합당한 경우 적극적인 신조직검사를 시행하여 원인 질환을 찾는 노력이 필요할 것으로 사료된다.. 남아가 75명으로 남녀비는 3.6:1을 보였다. 임상병리학적 분류상 일차성 신증후군이 89명(92.7%), 이차성 신증후군이 7명(7.3%)이었으며 일차성 신증후군에서 minimal change nephrotic syndrome이 71명으로 79.8%, focal segmental glomerulosclerosis 11.2%, mesangial proliferation 4.5%, membranoprolifrative glomeulonephritis 3.4%, membranous nephropathy 1명 1.1%이었으며 2차성 신증후군은 $Henoch-Sch\"{o}nlein$ nephritis가 3례로 가장 많았다. 미세변화 신증후군 71명 중 비재발군이 16명으로 22.5%, 비빈발 재발군 49.3%, 빈발 재발군 18.3%, 스테로이드 의존군 9.9%를 보였다. 결론 : 대전시에서의 신증후군 환아의 발생빈도는 15세이하 소아 10만명당 약 5명으로 추정되었으며 10여 년전과 비교하여 큰 변화를 보이지는 않았다. 또한 저자들의 임상병리학적 연구결과가 다른 문헌에서 보고된 소아 신증후군의 연구결과와 큰 차이를 보이지 않음을 알 수 있었다. 자극에 차이가 있지 않나 추측되며 이에 관한 추후 연구가 요망된다. 총대장통과시간의 단축은 결장 분절 모두에서 줄어들어 나타났으나 좌측결장 통과시간의 감소 및 이로 인한 이 부위의 통과시간 비율의 저하가 가장 주요하였다. 이러한 결과는 차가운 생수 섭취가 주로 결장 근위부를 자극하는

• Korean Journal of Ecology and Environment
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• v.38 no.2 s.112
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• pp.217-224
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• 2005

This study was aimed to analyze the long-term fluctuation of water quality and phytoplankton dynamics of Paldang reservoir in Korea and to assess the relationship between algal bloom patterns and hydrological, limnological data. Diatoms in Paldang reservoir occurred continuously through the year. Blue- green algae occurred during the summer season (from June to Sept.), and the highest count was observed in July. Occurrence pattern of green algae was similar to that of blue-green algae. The rest of algae contained a lot of Cryptomonas spp. whose concentration was high from May to Aug. Dominant algal genera (>>7,000 cells $mL^{-1}$) in Paldang reservoir were Aulacoseira, Cyclotella, Microcystis, and Cryptomonas spp. Microcystis and Anabaena occurred during the summer season. Many different green algal genera were found in Paldang reservoir but their abundances were very low. There were some significant correlations (r>0.3, p<0.05) between algal taxa and water quality; diatoms and water temperature, TP:blue-green algae and water temperature, pH, DO saturation, COD, TP; green algae and water temperature, pH, DO saturation, COD, SS, TP. Furthermore, algal genera and water quality was significantly correlated (r>0.3, p<0.05) ; Aulacoseira and TN, TP; Anabaena and water temperature, DO saturation, COD, TP : Microcystisand water temperature, pH, DO saturation, TP; Coelastrum and COD, SS; Scenedesmus and water temperature, COD, TN, TP; Cryptomonas and DO saturation, TN. In Paldang reservoir, the water temperature had relatively big effect on blue-green algal bloom that was also dependant upon its hydrologic condition.

• Korean Journal of Food Science and Technology
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• v.35 no.5
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• pp.877-883
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• 2003

Multi-degradable master hatch (M/B) was prepared and 0.05 mm polyethylene (PP) food packaging films containing 0, 10, and 20% M/B were manufactured by inflation film processing. The films were exposed to UV radiation, fungi, and heat in order to observe their photolysis, biodegradability, and thermal degradability, respectively. While pure PP film maintained more than 70% of its original elongation after 8 weeks of UV radiation, an almost perfect loss in the elongation of PP film containing 20% M/B was observed. Significant decreases in elongation of PP films by heat treatment $(68{\pm}2^{\circ}C)$ were also found in samples containing the multi-degradable M/B. By observing changes in film surface after the inoculation of fungi using scanning electron microscopy (SEM), the biodegradability of plastic film could be accelerated with the addition of multi-degradable M/B. The results of the mulching test in yard showed that adding multi-degradable M/B can effectively degrade plastic films in natural environmental conditions without interrupting the growth of plants.

• Korean Journal of Animal Reproduction
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• v.23 no.3
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• pp.247-256
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• 1999

The present study was performed to improve the reproductive disturbance as well as the elimination of microbiological contamination for animals bred under conventional conditions followed by in vitro fertilization and embryo transfer techniques including embryo and sperm freezing, using a mouse strain(M. m. molossinus-tt＠Kist) showing the abnormal behavior disorder derived from Korean wild mice (Mus musculus molossinus). Moreover, hematological and serum biochemical analyses were also carried out to obtain the basic data of this mouse strain The results are summarized as follows: 1. In comparison with hematological data, the numbers of RBC and platelet of this mouse strain were appeared as the higher value those that of the same aged inbred strains such as BALB/c, DBA/2, C57BL/6 and C3H /Hen. However, no differences were found in values of WBC, Hb and Ht. Moreover, total cholesterol of this strain showed a low value but triglyceride, total protein and albumin values were similar as in inbred strains. 2. The average numbers of superovulated oocytes treated with 2.5/2.5 IU and 5.0/5.0 IU of PMSG/hCG were 11.6 and 12.7, respectively. The fertilization rates of 2.5/2.5 IU PMSG /hCG treatment(87.9%) was higher than 5.0/5.0 IU treatment(52.0%) (p＜0.05) and the developmental rate of 2 cell stage embryos were 외 so appeared as higher value 99.0% and 90.6%, respectively. 3. The rates of in vitro fertilization treated with frozen sperm(24.8%) was significantly lower than of that fresh sperm(87.9%), (p＜0.05). 4. The five, six and ten heads of offspring were obtained from frozen-thawed 2 cell embryos by in vitro fertilized, 2 cell embryos from in vitro fertilized by frozen-thawed spermatozoa. and 2 cell embryos by in vitro fertilization, respectively. These offspring developed the expected disease about 2 weeks after birth, which was confirmed that the disease character of this mutant mouse strain was reliably reproduced. 5. MHV(Mouse hepatitis virus) and Staphylococcus aureus were successfully eliminated from conventional animals by in vitro fertilization-embryo transfer and the use of SPF recipient animals.

• Applied Microscopy
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• v.37 no.3
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• pp.157-166
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• 2007

This experiment was performed to evaluate the ultrastructural responses of the absorptive cells in the appendix of the mouse, inoculated with Ehrlich carcinoma cells in the inguinal area, following administration of BCG (Bacillus Calmette-Guerin). Healthy adult ICR mice weighing 25 gm each were divided into normal and experimental groups (experimental control group and BCG treated group). In the experimental groups, each mouse was inoculated with $1{\time}10^7$ Ehrlich carcinoma cells subcutaneously in the inguinal area. From next day after inoculations, 0.5mL of saline or BCG (0.5 mL/25gm B.W.: $0.03{\times}10^8{\sim}0.32{\times}10^8CFU$) were injected subcutaneously to the animals every other day. The day following the last injection, each mouse was sacrificed. Pieces of the tissue were taken from the appendix, prefixed with 2.5% glutaraldehyde-1.5% paraformaldehyde solution, followed by post-fixation with 1% osmium tetroxide solution. The ultrathin sections were stained with uranyl acetate and lead citrate. In the normal control, experimental control and BCG treated mice, general morphology of the absorptive cells of appendix were similar. But myelin figures and intramitochondrial dense granules were more frequently observed in the absorptive cells of BCG treated mice than normal control ones. Above results show that BCG did show slight ultrastructural alterations in the absorptive cell of the appendix. These results that BCG may slightly suppress function of the absorptive cells of the appendix.

• Korean Journal of Ecology and Environment
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• v.40 no.3
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• pp.379-386
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• 2007

Nonylphenol (NP) has been well known as a major substance of surfactant and/or estrogenic environmental hormone. We tested toxic effects of nonylphenol on the population growth and development of aquatic organism such as algae (Microcystis aeruginosa), heterotrophic nanoflagellate (Diphylleia rotans), micro- (Brachionus calyciflorus) and macro-zooplankton (Daphnia magna) among eutrophic water food-web constituents. Dosage of NP treatment were 4 to 5 grades, according to each organism's tolerance based on pre-experiments; algae (0.01, 0.05, 0.10, 1.00 mg $L^{-1}$) Diphylleia rotans (0.5, 1,2. 5,6, 10 ${\mu}g\;L^{-1})$, Brachionus calyciflorus (0.1, 0.5, 1, 2.5, 5 ${\mu}g\;L^{-1}$), and Daphnia magna (0.5, 1, 5, 10, 50 ${\mu}g\;L^{-1}$), respectively. Toxic effects were measured by the changes of biomass of each organism after NP treatment. All experiments were triplication. As suggested, the higher concentration of NP treatment, the stronger inhibited the population growth of all organisms tested. In view of toxicity, a variety of concentration of NP showed a significant growth inhibition to organism; algae to 0.05 $mg\;L^{-1}$, D. rotans and B. calyciflorus to 1.0 ${\mu}g\;L^{-1}$, and D. magna to 5.0 ${\mu}g\;L^{-1}$, respectively. The $EC_{50}$ of each organism to the nonylphenol are as follows; 3. calyciflorus (2.49 ${\mu}g\;L^{-1}$), D. rotans (3.49 ${\mu}g\;L^{-1}$), D. magna (7.61 ${\mu}g\;L^{-1})$, and M. aeruginosa (47 ${\mu}g\;L^{-1})$. NP toxic effects on the development of zooplankton like egg production showed some differences in treatment concentration between Brachionus calyciflorus ${0.1{\sim}1NP{\mu}g\;L^{-1})$ and Daphnia magna $(0.5{\sim}5NP\;{\mu}g\;L^{-1})$. These results suggest that a strong growth inhibition of predator or grazer by the nonylphenol can stimulate the algal growth, or can play important role in evoking the nuisance algal bloom in eutrophic water with enough nutrients.