• Title/Summary/Keyword: 면처리

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Biological Effects of Fibronectin Type III 10 domain on Human Osteoblast-like cells (Fibronectin type III 10 도메인이 조골양 세포에 미치는 생물학적 영향)

  • Lee, Chang-Seok;Jang, Jun-Hyeog;Kim, Tae-Il;Lee, Yong-Moo;Rhyu, In-Chul;Chung, Chong-Pyoung;Han, Soo-Boo;Ku, Young
    • Journal of Periodontal and Implant Science
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    • v.34 no.2
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    • pp.293-301
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    • 2004
  • 1. 연구 목적 Fibronectin은 세포외기질의 주요성분인 거대 당단백질로서, 조골세포의 부착과 증식 및 이동능에 중요한 역할을 담당한다고 알려져 있다. 이러한 fibronectin의 조골세포에 대한 영향을 실제 임상에 적용하기 위해서, 전체 fibronectin 단백질을 사용하는 것은 면역학적으로나 경제적으로 많은 단점을 안고 있어서, 유효한 반응단위만을 추출하여 활용하는 것이 바람직한 방법으로 알려져 있다. 이 연구의 목적은 세포부착에 주로 관여하는 fibronectin type III분절 중 10번 도메인이 조골양 세포에 미치는 영향을 전체 fibronectin단백질과 fibronectin type III 7-10 도메인 분절과 비교, 관찰하는 것이다. 2. 연구 방법 사람의 fibronectin을 기초로 한 적절한 primer로서, 유전자 재조합법을 이용하여 fibronectin type III 10 도메인과 fibronectin type III 7-10 도메인 분절을 얻었으며, 전체 fibronectin분자는 상용으로 준비하여 24-well 세포배양 용기에 도포하였다. 배양된 조골양세포(HOS cell)를 $1x10^5$ cells/well의 농도로 각 well에 분주하여 $37^{\circ}C$에서 1시간 배양을 하였다. Cell adhesion assay를 실시하기 위해 10% formaldehyde로 고정시키고 1% Crystal Violet으로 염색하여 광학현미경을 관찰 후 2% SDS를 처리하여 microplate reader기를 이용하여 570nm에서 혼탁정도를 측정하였다. 음성대조군으로는 RPMI 용액을 사용하였다. 동일한 방법을 이용하여 준비된 $35mm^2배양접시에 HOS cell을 $37^{\circ}C$에서 4일간 배양 후, MTS assay를 이용하여 세포 증식도에 미치는 영향을 관찰하였다. 6일째 405nm에서 활성화된 세포에서 분비된 p-nitrophenol을 이용한 alkaline phosphatase activity를 측정하였다. 3. 결과 및 고찰 Fibronectin type III 10 도메인은 HOS cell에 대한 생물학적인 효과면에서, 전체 fibronectin 분자 및 fibronectin type III 7-10 분절과 통계적으로 유사한 세포부착도를 보여주었으며, 세포증식도와 alkaline phosphatse 활성도면에서도 큰 차이가 나타나지 않았다. 이상의 연구결과로 볼 때, fibronectin type III 10 도메인이 조골세포의 증식을 목적으로 사용하는 생체재료의 표면개질 부착물질로 응용할 수 있는 가능성이 있다고 하겠다.

A cephalometric study on the relationship between mandibular opening movement and morphology of craniofacial skeleton (아동의 개구운동과 두개안면골격형태의 상관성 -측모두부방사선 계측법적 연구-)

  • Kim, Min-Shil;Chung, Kyu-Rhim
    • The korean journal of orthodontics
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    • v.30 no.3 s.80
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    • pp.297-306
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    • 2000
  • Lateral cephalometric X-ray films in maximal intercuspation and maximal opening of 68 children were taken and analyzed to examine the pattern of condylar movement and to study the relationship between opening movement and morphologic factors of craniofacial skeleton. The results were as follows : 1. The mean value of maximal opening capacity was 47.1mm, condylar moving distance was 18.1mm, horizontal condyle movement was 17.5mm, vertical condyle movement was 3.8mm and condylar moving angle was $13.1^{\circ}$. 2. The maximal opening capacity had positive relationship with the length of anterior cranial base, mandible and maxillary complex and with posterior facial height and had negative relationship with articular angle, sagittal jaw relationship. 3. Vertical condyle movement and condylar moving angle had positive relationship with articular angle and had negative relationship with gonial angle. 4. Horizontal condyle movement and condylar moving distance had positive relationship with the length of maxillary complex.

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Extraction and Complement of Hexagonal Borders in Corneal Endothelial Cell Images (각막 내피 세포 영상내 육각형 경계의 검출과 보완법)

  • Kim, Eung-Kyeu
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.3
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    • pp.102-112
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    • 2013
  • In this paper, two step processing method of contour extraction and complement which contain hexagonal shape for low contrast and noisy images is proposed. This method is based on the combination of Laplacian-Gaussian filter and an idea of filters which are dependent on the shape. At the first step, an algorithm which has six masks as its extractors to extract the hexagonal edges especially in the corners is used. Here, two tricorn filters are used to detect the tricorn joints of hexagons and other four masks are used to enhance the line segments of hexagonal edges. As a natural image, a corneal endothelial cell image which usually has regular hexagonal form is selected. The edge extraction of hexagonal shapes in corneal endothelial cell is important for clinical diagnosis. The proposed algorithm and other conventional methods are applied to noisy hexagonal images to evaluate each efficiency. As a result, this proposed algorithm shows a robustness against noises and better detection ability in the aspects of the output signal to noise ratio, the edge coincidence ratio and the extraction accuracy factor as compared with other conventional methods. At the second step, the lacking part of the thinned image by an energy minimum algorithm is complemented, and then the area and distribution of cells which give necessary information for medical diagnosis are computed.

Mechanical Characteristic Evaluation of Sn-Ag-Cu Lead Free Solder Ball Joint on The Pad Geometry (패드 구조에 따른 Sn-Ag-Cu계 무연 솔더볼 접합부의 기계적 특성평가)

  • Jang, Im-Nam;Park, Jai-Hyun;Ahn, Yong-Sik
    • Journal of the Microelectronics and Packaging Society
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    • v.17 no.2
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    • pp.41-47
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    • 2010
  • The effect of PCB and BGA pad designs was investigated on the mechanical property of Pb-free solder joints. The mechanical property of solder joint was tested by three different test methods of drop impact tests, bending impact test, and high speed shear test. Two kinds of pad design such as NSMD (Non-Solder Mask Defined) and SMD (Solder Mask Defined) were applied with the OSP finished Pb-free solder (Sn-3.0Ag-0.5Cu, Sn-1.2Ag-0.5Cu). in the drop impact test and bending impact test, the characterized lifetime showed the same tendency, and SMD design showed better mechanical property of solder joint than NSMD regardless of test method, which was due to the different crack path. The fracture crack on SMD pad was propagated along the intermetallic compound (IMC) layer of solder joint, while the fracture crack on NSMD pad propagated through upper edge of land which shields pattern. In the high speed shear test, pad lift occurred on the solder joint of NSMD. SMD/SMD combination of pad design consequently illustrated the best mechanical property of BGA/PCB solder joint, followed by SMD/NSMD, NSMD/SMD, and NSMD/NSMD.

Three Dimensional Comparative Study On The Accuracy Of Impression Technique Using New Impression Coping (새로운 인상용 코핑을 이용한 임플란트 인상법의 정확성에 대한 3차원적 비교 연구)

  • Kim, Se-Ra;Kim, In-Soo;Park, Sung-Jae;Lee, Byung-Ok;Ko, Sok-Min
    • Journal of Dental Rehabilitation and Applied Science
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    • v.26 no.3
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    • pp.323-337
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    • 2010
  • The objective of this study was to examine the availability of the new impression coping by comparing with conventional coping in implant pick-up impression technique. Five implant fixtures were installed on #14, 21, 23, 25, 27 in acrylic resin model. That model with 5 fixtures was standard model, which was divided 3 groups; using new flag type impression coping, conventional impression coping splinted with self-curing resin, conventional impression coping without splinting. We made metal circular cones for calculation 3-dimensional coordinates by attaching to implant fixtures or analogs. Three-dimensional relationships of each model were calculated. Data was analyzed by multiple ANOVA and Bonferroni. The accuracy of impression between using new flag type impression coping and conventional impression coping did not show differences in 3 - dimensional analysis.Within limitations of this study, the new flag type impression coping is available in implant pick-up impression technique.

Core network maintenance by NO.7 protocol analyzing (Core Network 유지 보수를 위한 NO.7 Protocol 감시 방안)

  • Yoo Jun-Mo;Kim Yun-Sung
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.42 no.1
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    • pp.49-60
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    • 2005
  • The Back Bone of gearing portion between Core Network System which is used in wire and wireless system is No.7 Protocol. ISUP( ISDN User Part), INAP handling in Intelligent Network and MAP gearing method for linking each Network Element System in mobile network have use of No.7 signaling. Therefore, it is require for system to detect the problem - operating problem occurs in Core Network which use No.7 signaling or the existing problem that is not recognized - in shortest time. This paper study out analyzing and measuring system for No.7 protocol and analyzing out system whether out of order or not. It can abstract No.7 Signaling Message and analyze system performance and report it. This system is connected in system No.7 HW Module which is in charge of No.7 Signaling. It is not offer Emulator function and Simulation function for any scenario but offer monitoring No.7 signaling. This system perform the function about No.7 MTP, ISUP, INAP and MAP and is offered several wire and wireless network Operator. It can detect and correct SW system, HW system and operating system problem. From now on, this system may offer the function about A-interface which is gearing between BSC and MSC, so it will perform to analyze generally and prompt effective operating for Core Network system.

Accuracy Enhancement using Network Based GPS Carrier Phase Differential Positioning (네트워크 기반의 GPS 반송파 상대측위 정확도 향상)

  • Lee, Yong-Wook;Bae, Kyoung-Ho
    • Spatial Information Research
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    • v.15 no.2
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    • pp.111-121
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    • 2007
  • The GPS positioning offer 3D position using code and carrier phase measurements, but the user can obtain the precise accuracy positioning using carrier phase in Real Time Kinematic(RTK). The main problem, which RTK have to overcome, is the necessary to have a reference station(RS) when using RTK should be generally no more than 10km on average, which is significantly different from DGPS, where distances to RS can exceed several hundred kilometers. The accuracy of today's RTK is limited by the distance dependent errors from orbit, ionosphere and troposphere as well as station dependent influences like multipath and antenna phase center variations. For these reasons, the author proposes Network based GPS Carrier Phase Differential Positioning using Multiple RS which is detached from user receiver about 30km. An important part of the proposed system is algorithm and software development, named DAUNet. The main process is corrections computation, corrections interpolation and searching for the integer ambiguity. Corrections computation of satellite by satellite and epoch by epoch at each reference station are calculated by a Functional model and Stochastic model based on a linear combination algorithm and corrections interpolation at user receiver are used by area correction parameters. As results, the users can obtain the cm-level positioning.

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Development of 3-D GIS based Expression System for Utilization of Submarine Mineral Resource Exploration Data (해저자원 탐사자료의 효율적인 활용을 위한 3차원 GIS 기반의 표출 시스템 설계 및 구축)

  • Kim, Dong Il;Kim, Kye Hyun;Lee, Sung Joo;Park, Yong Hyun
    • Spatial Information Research
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    • v.21 no.3
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    • pp.21-30
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    • 2013
  • Recently, interest of submarine mineral resources has been increasing from the depletion of land resources around the world and many countries are involving in submarine mineral resource exploration work. South Korea is also in progress of mineral exploration and relevant study to ensure the submarine mineral resources from around the Korean Peninsula. As a result, the submarine mineral resource exploration data have been increasing annually. A database and 2D GIS-based system have been constructed for the management of the data. However, submarine mineral resource exploration data is explored and created on the sea-bed. Consequently, the visual confirmation of the water levels and marine landform is important for high dimension analysis. Therefore, the major aims of this study are to collect marine landform data from around the Korean Peninsula and to develop 3 dimension GIS based system that is linked to the submarine mineral resource exploration data. In detail, marine landform data were acquired for the Korean Peninsula and they were interpolated into raster file format. The raster file was then processed to be easily used and was entered into an Oracle database. Based on this database, 3D expression and overlap function between marine landform data and submarine mineral resource exploration data were designed using ArcScene offered by the ESRI. After design, 3D GIS based expression system was developed. Confirmations of locations and changes in the submarine mineral resource exploration data based on 3D GIS are enabled to support the efficient application of the proposed system. It is expected that this system will be highly useful for estimating the reserves of mineral resources and for providing valuable information for economic evaluations.

A Study on Selection Attributes and Information Sources of Optical Shop (안경원 선택속성과 정보원천에 관한 연구)

  • Cha, Jung-Won
    • Journal of Korean Ophthalmic Optics Society
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    • v.21 no.3
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    • pp.173-179
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    • 2016
  • Purpose: This study is to help assist in the management of optical shops by using the importance sequence of optical shop selection attributes, which is related to the consumer's selection method of consumer, and by using the importance sequence of optical shop information sources which is related to a route for optical shop selection. Methods: Customer surveys were conducted from March 10 to March 31, 2015 targeting customers who have visited an optical shop in Seoul and Northern Gyeonggi-do regions. The analys method was descriptive statistics and data were analyzed by utilizing SPSS v.10.0 statistical package program. Results: The highest ranking five attributes among the importance of optical shop selection are "friendliness and politeness of staff", "cleanliness of an optical shop", "quick resolution of customer's complaints by staff", "eyes examination and glasses dispensing skill of staff", "customer's complaints and claims handling". The lowest ranking five attributes among the importance of optical shop selection are "provide free gifts", "scale or size of an optical shop", "opening time and closing time", "convenient parking facilities", "favorable countenance of staff". The two highestr ranking criteria among the importance of optical shop information sources are "previous utilization experience", "recommendation by a relative, a friend and a family etc". The two lowest ranking criteria among the importance of optical shop information sources are "advertisement" and "spatial exterior view of optical shop". Conclusions: It is shown that the important thing in management of an optical shop is an inner caliber like ability of ophthalmic optician, interaction with customers, and previous utilization experience rather than external factors like advertisement, exterior view, and bonus gift.

Effects of Circulation Fans on Uniformity of Meteorological Factors in Warm Air Heated Greenhouse (순환팬이 온풍난방 온실의 기상분포 균일화에 미치는 영향)

  • Yu, In-Ho;Cho, Myeong-Whan;Lee, Si-Young;Chun, Hee;Lee, In-Bok
    • Journal of Bio-Environment Control
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    • v.16 no.4
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    • pp.291-296
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    • 2007
  • This study was conducted to investigate the effects of horizontal air flow produced by circulation fans on horizontal and vertical profiles of meteorological factors. The three-dimensional distributions of air speed, air temperature, relative humidity and carbon dioxide $(CO_2)$ concentration were measured with and without the fans in operation. The uniformity of the spatial distribution of meteorological factors decreased as the outside air temperature decreased. In "fans off" condition, spatial variations of $4.7^{\circ}C$ in air temperature, 19% in relative humidity were detected. When the fans were operated, these variations were reduced to 2.2 and 6.3%, respectively. As the fan capacity increased, the difference in air temperature among sampling points decreased. The fan capacity of $0.0104m^3{\cdot}s^{-1}{\cdot}m^{-2}$ was enough to obtain a reasonable air flow in greenhouse. The vertical profiles of air temperature and $CO_2$ concentration were reasonably uniform regardless of measurement height and fan capacity. Further researches on the position of fans to reduce the difference in air temperature along the width and the effects of using a larger number of smaller fans are required.