• Journal of Oriental Neuropsychiatry
• /
• v.20 no.1
• /
• pp.75-87
• /
• 2009

목적 : 이 연구는 알쯔하이머 병에 대한 육미지황가미방의 효과를 조사하였다. 육미지황가미방은 산약, 산수유, 복령, 목단피, 구기자, 택사, 숙지황을 포함한 여러 가지 한약재의 전탕액으로 치매의 한약 치료제로 널리 사용되어 왔다. 방법 : 이 약물의 신경보호 작용을 조사하기 위해, 수중미로를 사용하여 학습과 기억에 대한 육미지황가미방의 효과를 평가 했고, 트리메틸틴은 강력한 유독물질로 선택적으로 중추신경계와 면역계의 세포를 파괴시킨다. 트리메틸틴(6.0 mg/kg, i.p.)주입 후, 쥐에게 육미지황가미방(400mg/kg, p.o.)를 2주 동안 날마다 복용시켰으며, 수중미로를 수행시켰다. 결과 : 육미지황가미방을 트리메틸틴에 노출시킨 쥐에 투약했으며, 그들은 수중미로에서 학습과 기억의 향상을 보였고, 이는 육미지황가미방이 어떠한 환경에서는 트리메틸틴으로 유발된 신경퇴화 후 중추신경계의 결손을 감소시킬 수 있음을 보여준다. 결론 : 이러한 결론은 육미지황가미방이 인지능력을 증가시키고, 트리메틸틴으로 유발된 신경퇴화에서 콜린 아세틸전환효소의 정도를 변화시킬 수 있음을 보여준다.

• Journal of Nutrition and Health
• /
• v.19 no.3
• /
• pp.177-189
• /
• 1986

This study was undertalien to investigate the effects of dietary protein sources on protein, lipid, Ca. metabolism and immune response in college women (20), 16 of whom had Jupito constitutions and 4 had Hespero constitutions based on Kwon's theory . They were divided into 3 groups according to the main dietary protein source ; Beef group, Yellow tailrunner group and Soybean durd group. The menu of experimental diet of 3 group were same except their main dietary protein source(beef, yellow tailrunner, soybean curd). They were fed experimental diet for 4 days and their food intake was not restricted. Beef group in Jupito constitutions and yellow tailrunner group in Hespero constitutions were expected to present better effects than the other groups, because beef and yellow tailrunner are good for Jupito constitutions and Hespero constitutions, respectively. Results of Beef group in Jupito constitutions and Hespero constitutions, respectively. Results of Beef group in Jupito constitutions and yellow tailrunner group in Hespero constitutions were as following. 1) Calcium retention rate, Calcium apparent digestibility, serum Complement 3 concentration and LDL+ VLDLconcentration were higher in both groups. 2) Serum HDL concentration and immunoglobulin G concentration were lower in both groups than in the other groups. According to the main dietary protein sources, noted results were as following. 1) Serum total lipid and total cholesterol concentration were the significantly lowest in Soybean curd group. 2) Nitrogen retention rate was the significantly highest in Beef group.

• Korean Journal of Veterinary Research
• /
• v.20 no.1
• /
• pp.45-51
• /
• 1980

Cyclophosphamide(CY)가 마우스 및 가토(家兎)의 P. multocida 야외분리균주(野外分離菌株)의 호흡기관내(呼吸器管內) 인공감염(人工感染)에 대한 감수성(感受性)에 미치는 영향(影響)을 연구(硏究)하였다. 체중(體重) Kg당(當) 300mg CY를 주입(注入)한 마우스와 150mg CY를 처리(處理)한 가토(家兎)는 P. multocida의 호흡기관내(呼吸器管內) 인공감염(人工感染)에 대해서 높은 감수성(感受性)을 보였다. 또한 CY 처리(處理)는 일시적(一時的)으로 말초혈액(末梢血液) 중에 있는 총백혈구수(總白血球數)와 단핵백혈구수(單核白血球數)의 감소(減少)를 야기(惹起)했고, 비장(脾臟)의 백수(白髓)와 적수(赤髓)에 있는 임파구(淋巴球)의 소실(消失)을 초래(招來)했다. CY 주사(注射)된 가토(家兎)는 접종(接種)된 균주(菌株)에 대한 항체형성능력(抗體形成能力)의 저하(低下)를 나타냈다. 얻어진 결과(結果)는 가토(家兎)에 있어서 면역기능(免疫機能)의 저하(低下)와 호흡기질병(呼吸器疾病) 발생(發生) 간(間)의 상호관계(相互關係)에 대해 고찰(考察)하였다.

• The Korean Journal of Pain
• /
• v.2 no.1
• /
• pp.94-96
• /
• 1989

The cases of continuous epidural catheterization for pain control have increased nowadays. Epidural abscess, one of the complications of continuous epidural catheterization. can cause severe and permanent neurologic deficit. Though the incidence of epidural abscess is rare, we should devote every effort to prevention of abscess formation. And in the occurrence of epidural abscess formation, treatment should be started as early as possible with antibiotic therapy and surgical drainage to prevent permanent neurologic deficit. We experienced a case of epidural abscess following continuous epidural catheterization, and so the case is presented.

• Journal of dental hygiene science
• /
• v.6 no.1
• /
• pp.41-45
• /
• 2006

The objective of this research was to analyze and evaluate pre-dental hygiene curriculum of dental hygiene education program in USA and Canada. Sixty one programs were searched through their website. The curriculum include content in the four areas: general education, biomedical science, dental science, dental hygiene science. Pre-dental hygiene curriculum include content in general education and biomedical science. General education content include oral and written communications, psychology, and sociology. Biomedical science content include anatomy, physiology, chemisrty, biochemistry,immunology,general pathology, nutrition, and pharmacology. Pre-dental hygiene curriculum content provide the foundational knowledge and skill such as competencies to communicate effectively, to participate community health program, and to make decisions regarding dental hygiene service.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2018.05a
• /
• pp.53-57
• /
• 2018

고도의 산업화에 따라 도시의 성장과 인구의 밀집으로 인하여 물의 수요가 증가하고 이에 따라 각종 오염원의 유입이 증가함에 따라서 적절한 수질 관리에 대한 필요성이 대두되고 있다. 그리고 산업이 다양화됨에 따라 배출되는 하 폐수의 특성도 다양해져 기존의 수질처리장은 효율적인 처리를 하는데 있어 많은 어려움이 발생되고 있다. 굴 패각은 하수처리의 담체로 이용할 경우 살수여상의 문제점 중 하나로 나타나는 막힘 현상을 다소 감소시킬 수 있고, 다공질체로서 표면적이 불규칙하고 비표면적이 크기 때문에 반응기내에 공기를 원활하게 공급할 수 있으며, 중금속 이온과 유기물에 대한 흡착 효율이 뛰어나 미생물이 쉽게 부착, 성장할 수 있는 장점을 가지고 있다. 천연광물질은 전 세계적으로 발견되어 활용된 역사가 짧지만, 그 특징은 산소결핍보충, 유해원소 흡착 성능, 반도체 작용, 인체 면역력 강화, 바이러스성 질병의 예방, 중금속 해독 등의 다양한 특징으로 환경호르몬 제고, 음이온 원적외선 발생으로 세포의 노화방지, 인체 노폐물 제거와 같은 특징을 지닌 매우 유용한 자원으로 확인되고 있으며, 수질정화 능력이 탁월한 것으로 보고 있다. 이에 본 연구는 어촌폐기물인 굴 패각과 수질정화에 탁월한 천연 광물질을 혼합하여 경제적이고, 효율성이 높은 다공성 수질개선 담체를 개발하고, 수목여과용 비점오염시설 장치에 적용하여 혼합 담체가 수처리 시설의 담체로서 사용이 가능한지, 수목여과용 비점오염시설 장치에 적용할 수 있는지 검토하고자 수행하였다. 이 연구는 2017년 중소기업청 기술개발사업에서 연구지원을 받아 연구되었습니다.

• Clinical and Experimental Reproductive Medicine
• /
• v.35 no.4
• /
• pp.247-265
• /
• 2008

Objectives: Many types of liver diseases can damage regenerative potential of mature hepatocytes, hepatic progenitor cells or oval cells. In such cases, a stem cell-based therapy can be an alternative therapeutic option. We examined whether human amnion-derived mesenchymal stem cells (HAM) and human umbilical cord-derived stem cells (HUC) could differentiate into hepatocyte-like cells as therapeutic cells for the liver diseases. Methods: HAM and HUC were isolated from the amnion and umbilical cord of the volunteers after a caesarean section with informed consent. In order to differentiate these cells into hepatocyte-like cells, cells were cultivated in hepatogenic medium using culture plates coated with fibronectin. Effects of hepatocyte growth factor, L-ascorbic acid 2-phosphate, insulin premixture fibroblast growth gactor 4, dimethylsulfoxide, oncostatin M and/or dexamethasone were examined on the hepatic differentiation. After differentiation, the cells were analyzed by RT-PCR, immunocytochemistry, immunoblotting, albumin ELISA, urea assay and periodic acid-schiffs staining. Results: Initial fibroblast-like appearance of HAM and HUC changed to a round shape during culture in the hepatogenic medium. However, in all hepatogenic conditions examined, HUC secreted more amounts of albumin or urea into medium than HAM. Expression of some of hepatocyte-specific genes increased and expression of new genes were observed in HUC following cultivation in hepatogenic medium. Results of immunocytochemistry and immunoblotting analyses demonstrated that HUC secreted albumin into the culture medium. PAS staining further demonstrated that HUC could store glycogen inside of the cells. Conclusions: Both HUC and HAM could differentiate into albumin-secreting, hepatocyte-like cells. Under the same hepatogenic conditions examined, HUC more efficiently differentiated into hepatocyte-like cells compared with the HAM. The results suggest that HUC and HAM could be used as sources of stem cells for the cell-based therapeutics such as in liver diseases.

• The Korean Journal of Zoology
• /
• v.39 no.3
• /
• pp.257-265
• /
• 1996

Previously, we demonstrated that estradiol (E$_2$) was produced by medium sized follicles of Rana nigromaculata and Rana dybowskii in vitro. Present experiments were carried out to determine when the growing follicles have obtained the ability to produce E$_2$. Follicles in different growth stages were isolated and cultured for 6 hours in the presence or absence of frog pituitary homogenates (FPH, 0.1 pituitary/2m1) or various steroid precursors (200 ng/2m1). levels of progesterone (P$_4$), 17 -hydroxyprogesterone (17$\alpha$-OHP), androstenedione (AD), testosterone Cr) or E$_2$ in the medium were measured by RIA. The smallest follicles failed to produce steroids, whereas the smaller follicles produced considerable amounts of steroids (211 pg/follicle), and the medium sized follicles produced a large amounts of steroids (1653 pg/follicle) in response to FPH. Addition of pregnenolone (P5) resulted in a marked increase in P$_4$ but not in other steroids by the smallest follicles whereas the treatment resulted in a marked increase in P$_4$, 17$\alpha$-OHP, AD, T and E$_2$by the smaller and medium follicles. When the amounts of steroids are calculated on the basis of unit surface area (pg/mm$_2$), the ability of the smallest follicles to produce P$_4$ from P5 was similar to those of smaller and medium sized follicles. However, the smallest follicles failed to metabolize P$_4$ to other steroids whereas the smaller and medium follicles did. Taken together, the data suggest that the smallest follicles do not response to FPH in terms of steroid production but they have capacity to convert P5 to P$_4$ and that the smaller follicles have potential to produce E$_2$ although much less efficient than medium sized follicles.

• Journal of Life Science
• /
• v.21 no.5
• /
• pp.684-692
• /
• 2011

The present study furthered this research by determining the optimal level of inuloprebiotics derived from Korean Jerusalem artichoke to promote growth in broiler chickens. On hatch day, 320 male Ross 308 broilers were randomly allotted to four groups for a 35-day feeding regimen. The groups were a control (no supplementation), diet supplementation with antibiotics (8 ppm avilamycin), supplementation with 200 ppm inuloprebiotics, and supplementation with 250 ppm inuloprebiotics. Body weight and feed intake were comparably high in the two inuloprebiotics groups, and exceeded the values for the control and antibiotics groups. Body weight and feed intake was higher in chickens fed the antibiotics supplemented diet, compared to controls. There were significant differences among treatment groups. Feed conversion ratio during total experimental periods was significantly high in the control group compared to the inuloprebiotics-fed groups, but no significant difference was evident using antibiotics or inuloprebiotics dietary supplementation. Carcass weight, dressing percentage, and weight of breast and thigh muscle were significantly higher for inuloprebiotics-fed chickens, compared with control and antibiotic-fed chickens. Inuloprebiotics concentration was not significant, while antibiotics-fed chickens displayed significantly higher values than control chickens. Abdominal fat was significantly reduced (19.08%-23.30%) and blood immunoglobulin and the weight of the thymus and Bursa of Fabricius were significantly increased in chickens receiving inuloprebiotics-supplemented feed, compared to control and antibiotics fed groups. In the lower intestine, the viable counts of beneficial bacteria (Bifidobacteria and Lactobacillus) were significantly increased and counts of harmful bacteria (Escherichia coli and Salmonella sp.) were significantly decreased in the presence of the inuloprebiotics, compared to control and antibiotic. The present results support 200 ppm as an optimal level of Korean Jerusalem artichoke-derived inuloprebiotics as a dietary supplement to improve growth in broiler chickens.

• Journal of Life Science
• /
• v.34 no.7
• /
• pp.485-492
• /
• 2024

Sea cucumbers contain more than 50% protein in their solid content, and they also possess various bioactive substances such as saponins and mucopolysaccharides. This study analyzed the activities of various enzymes derived from Bacillus and lactic acid bacteria and determined to degrade the components of sea cucumbers. Among the analyzed strains, B. subtilis K26 showed the highest activities in protease and xylanase and relatively high activity in cellulase. Accordingly, samples of sea cucumber and water were mixed in equal proportions, sterilized, and then fermented by inoculating them with B. subtilis K26. Following this, a higher amino acid content was observed between 1.5 and 7.5 hr, a lower residual solid content in this time, and a lesser fermentation odor. The saponin content in fermented sea cucumber powder extracted with butanol was measured to be 1.12 mg/g. The chondroitin sulfate content was evaluated to be 5.11 mg/g in raw sea cucumber. The total polyphenol content, flavonoid content, and antioxidant activities were 6.95 mg gallic acid equivalent/g, 3.69 mg quercetin equivalent/g, and 3.69 mg quercetin equivalent/g in raw sea cucumber, respectively. Moreover, the DNA damage protective effect of fermented sea cucumber extract was found to be concentration-dependent, with a very strong effect at very low concentrations. Overall, we suggest that sea cucumber fermented with B. subtilis K26 has a high potential as a food for inhibiting oxidation, enhancing immunity, and improving muscle function in the human body thanks to its high free amino acid content.