• Parasites, Hosts and Diseases
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• v.27 no.2
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• pp.79-86
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• 1989

A pathogenic free-living amoeba, Naegleria fowleri, causes primary amoebic meningoencephalitis to human and experimental animals. This infection is rare, but the mortality is very high. Nowadays, drug treatment or active immunization of human or mice are being tried with partial effectiveness. This study shows passive immunization effect by transfer of immunized spleen cells, serum, or milk from immunized mother in mouse experimental model. Young BALB/c mice were immunized intraperitoneally with $2~3{\times}10^{6}$ trophozoites of N. fowleri, and spleen cells and sera were collected for injection to recipient mice. There were seven transfer groups, i.e., immunized mouse serum, spleen cells, serum and spleen cells, normal mouse serum, spleen cells, serum and spleen cells, and control group. Three days later, BALB/c mice were inoculated with $1{\times}10^{4}$ trophozoites of N. fowleri intranasally. After infection, decreased mortality ana prolonged survival time of mice were noted in immunized Bloops compared with non.immuniBed control group. The groups Injected with immunized spleen cells or normal serum shewed lower moltality than that of controls bult showed no changes of Serum IgG level. The groups injected with immunized serum or normal spleen cells showed increased serum IgG level after immunization but hundred percent mortality was observed. Mother mice were ifnfnunised increperitqneeliy with $2~3{\times}10^{6}$ trephozoites of N. fowleri at the end of pregnancy and weaning Period. Soon after the delivery, Jitters born of non-immunszed mother were matched with immunized mother for feeding immune milk. After three weeks, the litters were infected with $1{\times}10^{4}$ trophozeites of N. fowleri or sacrificed for serum collection to measure the IgG levels. The results show that anti-JV. fowleri IgG from mother was transferred to litter through milk but this IgG did not inauence the mortality or survival time of the infected mice.

• Journal of the korean veterinary medical association
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• v.29 no.11
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• pp.641-645
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• 1993

소 유방염에서 cytokine의 역할연구는 이제 시작단계이다. 근래 시험에서는 재결합 cytokine은 실세포 증가와 염증반응의 조절에 의하여 강화된 세균의 정화능력을 유방에 준다. 그리고 효과인자 세포의 보충결합 효과를 통하여 유방염의 결과를 조절하는 능력이 있다는 것을 제시한다. 현재 앞서가는 연구자들의 도전은 세균의 병원성 증가, 숙주반응이 유선에서 병원균 제거 요구 그리고 cytokine이 이들 반응을 조절할 수 있는 기전, 이들 사이의 상호기전을 더 이해하기 위한 것이다. 이 분야에서 앞으로의 발전은 시험보다 야외상태에서 전달되어지는 것이다. 그리고 효과적인 정보의 증가를 위하여 우리의 관심이 더 많아야 하는 것이고, 유선면역기능 조절이 소 유방염 관리를 위하여 앞으로 cytoki-ne의 치료응용에 큰 잠재력을 제시하는 것이다.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.10a
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• pp.124-125
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• 2002

현재 수산 양식업계에서는 질병에 대한 치료 또는 예방 효과가 있는 기능성 물질에 대한 관심이 고조되고 있다. 서구 여러 국가에서 지대한 관심을 모으고 있는 다당류인 “$\beta$-glucan” 은 숙주의 면역 활성을 향상시키는 기능을 이용하여 양식 어류의 사료에 혼합하여 양식어에 공급함으로써 화학 항생물질 및 항균 물질의 대체효과도 기대하고 있으며 그 생산성도 향상시키고 있다. (중략)

• Journal of the Korean Professional Engineers Association
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• v.38 no.1
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• pp.88-90
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• 2005

National medical bill is amounting 31 trillion, equivalent to about 8% of GDP. And Japanese poeple are beginning to doubt the western medical system that depends upon drugs and operations. They are seeking another method of treatment. It is well known the forest has many healthy and recreation benefit. Now I would like to talk about the benefits of forest therapy.

• Journal of Mushroom
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• v.2 no.4
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• pp.169-174
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• 2004

A hot water extract(HWE-P4) was separated from the fruit bodies of PMO-P4, and its antitumor and immunomodulatory activities against sarcoma-180 in ICR mice were investigated. The internal transcribed spacer(ITS) regions from PMO-P4 were amplified using polymerase chain reaction(PCR) and sequenced. The results revealed that PMO-P4 was belong to the Phellinus baumii. When oral administration at the dose of 160mg/kg/day in the mice until the end of the experiment with 2 week's pre-feeding of the HWE-P4, the survival rate of the mice was 152% for 50days after the inoculation of sarcoma-180 and the suppression rate of the tumor growth was 35.3%(p<0.05) for 28 days after inoculation of sarcoma-180. The HWE-P4 increased 71.4% of the CD4/CD8 ratio and 5-fold of the expression of CD25(IL-2 receptor chain) compared with the control. From these results, the antitumor activity of HWE-P4 is exerted through its immunomodulating activity on the host's immune system.

• Parasites, Hosts and Diseases
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• v.36 no.2
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• pp.127-132
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• 1998

Immunotherapy has been used in support of trimethoprim-sulfamethoxazole treatment for Pneumocvstis corinii pneumonia. The present study investigated the therapeutic or preventive effects of heterogeneous hyperimmune IgG antibody (HIA) in experimental rats. Their immunity was suppressed by steroid injection, and they were also injected peritoneally with HIA which reacted with 40-55, 92, 116, and 200 kDa bands of the crude antigen. All rats were infected by p. ccrinii and the cystic forms on lung impression smears were counted. The count was 20.5-76.5 (mean 52.5 ± 19.)1 in those which received steroid only, but decreased to 6.0-21.0 (mean 13.5 : 10.6) in those of group 3 which received HIA for the same duration. In other groups, the mean count ranged from 29.9 t 32.9 to 54.1 t 47.7, and in those which received 13.7 mg HIA the reduction effect was greater than in those which received 6.8 mg or 20.5 mg HIA. The present finding confirmed that in rats during the early stage of infection, the heterogeneous HIA to MSG antigen bands had a partial effect on p. cori,nii pneumonia, both prophylactically and therapeutically.

• Proceedings of the KSCN Conference
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• 2004.05a
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• pp.392.2-394
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• 2004

최근 우리나라는 노인인구가 급격히 증가되고 있고, 주요사망원인이 만성퇴행성 질환으로 이동하고 있다. 노화는 산화-환원의 균형이 깨져 산화 촉진쪽으로 반응이 진행되어 일어나며, 이러한 산화적 스트레스의 증가는 당뇨병이나 고혈압과 같은 만성질환의 유병율을 높이고, 또한 면역기능에도 영향을 미친다. 그러므로, 노화 억제 및 만성질환의 예방을 위한 항산화 영양소의 건강증진 효과에 대한 연구가 매우 중요하다. 따라서 본 연구는 우리나라 노인들의 항산화 영양상태를 평가하고, 만성질환 중 유병율이 높은 당뇨병, 고혈압에 따른 항산화 영양상태와의 관련성 및 노인들에게서 감소하는 것으로 알려진 면역기능과의 관련성을 조사하기 위해 3차에 걸친 연구를 수행하였다.(중략)

• Korean Journal of Medicinal Crop Science
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• v.16 no.5
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• pp.294-300
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• 2008

This study was performed to investigate the increasing of immune activities from Centella asiatica L. Urban by several solvent extraction with ultrasonification process. Ethanol extracts at $60^{\circ}C$ and associated with ultrasofication showed more 15% yield than normal $100^{\circ}C$ water extracts. And ethanol extracts showed higher yield than water extraction at each process. It can be concluded that yield due to polarity of compound in C. asiatica from HPLC peak. All of extracts from C. asiatica revealed lower cytotoxicity than 32%. The ethanol extracts with ultrasofication showed higher promotion on human B and T cell growth, about 10% compared to the control. The secretion of IL-6 and TNF-a was also enhanced as $4.86\;{\times}\;10^{-4}\;pg/cell$ and $5.73\;{\times}\;10^{-4}\;pg/cell$ each, by the addition ethanol extracts with ultrasonification to T cell. NK cell activation was improved up to 10% higher than the control, through adding the samples. It can be concluded that the ethanol extracts from C. asiatica has higher immune activities followed by ultrasonification process, possibly, by higher yield than conventional extraction process.

• Journal of Aquaculture
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• v.11 no.4
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• pp.495-503
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• 1998

The present study investigated the effects of Bisroot, that contains live bacteria (Bacillus polyfermenticus, Bacillus mesentericus, Streptococcus faecalis, & Bifidobacterium breve) and digestive enzymes (protease, lipase), on the growth, body composition and immune response of Nile tilapia fingerlings. One percent of the Bisroot was added to the experimenta feed. All exprimental fish were fed for 60 days. The weigh gains among the experimental fish were not significntly different (P>0.05). Hematocrit value, hemoglobin, total protein, glucose, GOT, and GPT were unaffected by Bisroot treatment. However, it was observed that glucose, GOT, and GPT value in the fish that were fed Bisroot, were lower than the control. The complement activity ($CH_50$) tended to be significantly increased by Bisroot treatment, but not lysozyme activity. Phagocytosis and respiratory burst activities of macrophages in the head kidney were enhanced by Bisroot. Therefore, the Bisroot diet enhances the cellular immune activities were enhanced by Bisroot. Therefore, the Bisroot diet enhances the cellular immune activities of non-specific immune responses. When fish were challenged with a virulent strain of Edwardsiella tarda, the Bisroot treated fish were more resistant than the control. The present results suggest that the introduction of Bisroot into the diet of Nile tilapia could increase their resistance against bacterial infection, reduce fish mortality, and offers economic benefits.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.104-104
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• 2019

표고(Lentinula edodes)에 다량 함유되어 있는 아미노산은 면역효과와 밀접한 관련이 있는 것으로 알려져 있다. 표고 특유의 향미에 대한 소비자들의 선호도가 상이하여, 표고를 맥아로 당화하여 소비자들의 섭취 편이성을 향상시키고, 아미노산을 포함한 당화액을 제조하여 면역효과가 증강된 제품을 개발하고자 본 연구를 수행하였다. 또한 버섯류를 비롯한 담자균류에는 다양한 약효성분이 함유되어 있어 민간에서도 자주 사용된 기록이 있다. 최근 고등담자균류인 버섯의 성분 및 유용성에 관심이 크게 증가하고 있는 추세이며, 이에 따라 버섯류에 대한 식품학적 및 약리학적 측면에서 연구가 활발히 진행되고 있다. 표고(L.edodes)와 당화효소로 사용한 맥아의 비율에 따라 A (표고 1 kg : 맥아 50 g), B (표고 1 kg : 맥아 100 g), C (표고 1 kg : 맥아 200 g), D (표고 1 kg : 맥아 300 g), E (표고 1 kg : 맥아 300 g) 5개의 시험구를 설정하였으며, 제조된 표고당화액을 농축 후 동결건조 하여 분말로 분쇄 후 시료로 사용하였다. 표고당화액의 면역효과를 검증하고자, Raw 264.7에 표고당화추출분말 A, B, C, D, E를 각각 10, 50, 100 및 $500{\mu}g/mL$의 농도로 처리하여 각 시료에 대한 세포 독성과 항염증효과를 확인하였다. 세포독성 시험결과, E 시험구의 경우 $100{\mu}g/mL$의 농도에서 75.3%, $500{\mu}g/mL$의 농도에서 66.3%의 세포생존율을 나타내 독성을 보였다. 또한 A, B, D를 각각 $500{\mu}g/mL$의 농도로 처리 했을 때, 94.1%, 83.1%, 80.2%의 NO 생성율을 나타내어 E 시험구를 제외하고는 세포독성이 없는 것으로 확인되었다. 표고당화추출분말의 구성아미노산 함량은 C 시험구에서 19,699.01 mg%로 가장 높게 나타났으며, A 시험구에서 17,231.14 mg%, B 시험구에서 17,152.38 mg%순으로 높게 나타났다. E 시험구에서 13,367.98 mg%로 가장 낮게 나타났다. 모든 표고당화추출분말에서 총 16종의 유리아미노산 이 검출 되었으며, C 시험구에서 2,562.06 mg%로 가장 높게 나타났고, B 시험구에서 2,243.16 mg%, D 시험구에서 1,895.29 mg%, A 시험구에서 1,672.82 mg%순으로 높게 나타났다.