• Monthly Duck's Village
• /
• s.68
• /
• pp.48-56
• /
• 2009

현대와 같이 산업사회로 발달되기 이전의 자연생태계는 행복하였다. 청정한 태양 빛 아래 맑은 공기, 물, 흙과 나무가 어우러진 자연의 환경에서 엄연한 먹이사슬질서 속에서 살아 왔었는데, 인구증가와 산업사회로 발전하면서 대량수요가 요구되면서 그들은 점차 행복한 삶의 터전이 없어지고, 강제로 억제시킨 극도의 열악한 집단사육환경에 적응하면서 살아갈 수밖에 없게 되면서 먹이사슬에서 부작용과 역기능이 발생되기 시작하였다. 집단사육장환경을 획기적으로 개선해야 된다. 태양빛이 완전 차단되고 밀폐된 공간에서 집단사육시키고 있는 닭, 오리, 돼지가 정상적으로 생장할 수 있도록 적어도 자연환경에 가까운 밝고 청정한 환경으로 개선시켜야 된다. 사육동물들이 정상적으로 생장할 수 있도록 자연환경과 버금가는 생태환경으로 개선시켜 주어야만 오리들이 건강하게 성장하고 면역력이 증강되어 조류인플루엔자와 같이 원인불명의 질병에 감염되지 않을 것이다. 현실적으로 모든 실내 생태계의 성장을 활성화시키면서 면역력을 증강시킬 수 있는 생명에너지 빛(Bioenergetic Rays)을 방사하는 차세대 조명기술로 집단사육장의 실내를 획기적으로 밝게 하고, 실내의 탁한 공기가 신선한 외부공기로 충분히 순환이 될 수 있도록 반 밀폐구조로 개조해야 한다. 그리고 한 마리 당 활동공간을 넓혀 주어야 한다.

• Journal of fish pathology
• /
• v.19 no.2
• /
• pp.173-181
• /
• 2006

Miamiensis avidus is a scuticociliate causing mortality in olive flounder Paralichthys olivaceus. To evaluate immune response of olive flounder against M. avidus, 2.6×106cells/fish of Formalin killed cell (FKC) was intraperitoneally (i.p.) injected, and 2.4 × 106cells/㎖ of sonicated FKC was immersion immunized to 14.9 cm (26.8g) fish. Fish were immunized 2 times with 2 weeks intervals. Antiserum from immunized fish caused agglutination and immobilization of the ciliate. In ELISA test, immunized group exhibited higher titers than control group. In addition, i.p. immunized fish showed higher phagocytic ratio, phahgocytic index and chemotaxic activity. To evaluate in vivo efficacy of FKC on fish, 1.43 × 105cells/fish was i.p.. injected, and 2.2 × 105 cells/㎖ of sonicated cell was immersed into 8.6cm (6.3g) fish. After 2 times immunization with 2 weeks intervals, fish were infected with 2.0 × 104 and 2.0 × 103 cells/ml of live ciliates by immersion. After 3 weeks, cumulative mortality was lower in the i.p. immunized group and mortality was delayed in immersion immunized group. In conclusion, specific immune response of oliver flounder against M. avidus was elevated after immunization and these immune response may prevent and/or delay for the M. avidus infection to olive flounder.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.48 no.4
• /
• pp.466-473
• /
• 2015

The juvenile red variant of the sea cucumber Stichopus japonicus is distributed worldwide. It is a valuable food source in Korea, China, and Japan. Major issues in farming the juvenile red variant sea cucumber include growth rates and disease resistance. In recent years, studies have focused on feed additives to enhance immune system and health. In this study, we used a common diet (CD), a nutritional diet (ND; mixed nutritional supplements), and an immunity diet (ID; mixed probiotics, spirulina, and levan) and compared the growth rates and immunity of juvenile red variant sea cucumbers fed the experimental diets for 12 weeks. The growth and survival rates in the ID group were significantly increased (P>0.05). This suggests that the feed additives positively influenced immunity and growth in the ID group. However, the immune activity was exhibit a stabilizing effect, and further investigation of immune effects is required.

• Proceedings of the Ginseng society Conference
• /
• 2006.05a
• /
• pp.59-59
• /
• 2006

• 한국생물공학회:학술대회논문집
• /
• 2002.04a
• /
• pp.551-552
• /
• 2002

• Proceedings of the Korean Society of Veterinary Service Conference
• /
• 2000.06a
• /
• pp.177.1-177
• /
• 2000

• Microbiology and Biotechnology Letters
• /
• v.26 no.2
• /
• pp.130-136
• /
• 1998

An Immunostimulating polysaccharide was produced from the suspension culture of Angelica gigas H4, plant cells. In order to enhance the polysaccharide production by the A. gigas cell culture, medium composition and physical conditions were optimized. Schenk and Hildebrandt (SH) medium was selected as an optimal basal medium for the growth of A. gigas. The maximum cell and polysaccharide concentration obtained in SH medium were 15.8 g DCW/l and 0.85 g polysaccharide/l, respectively, at $25^{\circ}C$ under dark condition. For the enhanced polysaccharide production, a polysaccharide production medium (PPM) was established by modifying Gamborg B5 medium with optimized carbon sources, growth regulators, organic and inorganic elements. Optimal initial pH and temperature were 6.0-6.6 and $20^{\circ}C$, respectively, and the dark condition was better than the light condition. The maximum polysaccharide concentration of 1.5 g/l could be obtained through the optimization of the medium composition and physical conditions.

• Environmental Analysis Health and Toxicology
• /
• v.22 no.4
• /
• pp.287-297
• /
• 2007

유산균(Lactic acid bacteria)은 Escherichia coli와 Salmonella typhimurium과 같은 병원균에 항균 활성을 지니며 면역 증강효과를 나타내는 등 건강에 이로운 다양한 역할을 한다. 유산균에 의한 항균 효과는 E. coli와 S. typhiumurium에 대항하는 항균 활성으로 측정되어 졌으며, 면역 증강 효과는 유산균을 처리한 RAW264.7 대식세포의 활성화로 측정하였다. Lactobacillus acidophilus, Streptococcus thermophilus, Bifidobacterium bifidum의 E. coli와 S. typhimurium에 대한 항균활성은 9시간 이상 혼합 배양하였을 때 가장 좋은 항균효과를 나타내었고 E. coli와 S. typhiumurium 두 균주가 9시간 이후에는 모두 콜로니를 형성하지 않았다. RAW264.7 세포는 유산균에 의한 NO와 $TNF-{\alpha}$의 생성과 대식세포의 형태 변화를 알아보기 위한 대식세포로서 이용되었다. NO와 $TNF-{\alpha}$의 생성은 유산균을 처리한 RAW264.7세포의 24,48시간 배양 시 농도 의존적으로 증가하였고 대식세포의 형태 변화 역시 유산균에 의해 영향을 받았음을 확인할 수 있었다. 이를 통하여 (주)쎌바이텍으로부터 분양받은 유산균은 항균활성과 대식세포의 활성을 유도하여 면역을 증강시키는 효과를 지니고 있음을 in vitro 실험을 통해 확인하였다.

• Journal of Life Science
• /
• v.20 no.4
• /
• pp.623-631
• /
• 2010

Hericium erinaceus, an edible and medicinal mushroom belonging to the Basidiomycota family, has been used for curing gastric ulcers and stomach cancers in human beings and is also known to have good inhibitory effects on sarcoma 180 and Ehrlich carcinoma in mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to as Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from the fruiting body of the mushroom. In in vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cell lines such as Sarcoma 180, HepG2, HT-29 and NIH3T3 at concentrations of $10{\sim}2,000\;{\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited a life prolongation effect of 29.1~54.1% in mice previously inoculated with Sarcoma 180. Fr. Na increased the numbers of spleen cells by 2.9 fold at a concentration of $50\;{\mu}g/ml$ compared with the control. Fr. Na improved the immuno-potentiating activity of B lymphocytes by increasing alkaline phosphatase activity by 5.5 fold compared with the control at a concentration of $200\;{\mu}g/ml$. Fr. NaCl increased the numbers of peritoneal exudate cells and circulating leukocytes by 4 and 2.3 folds at a concentration of 50 mg/kg, respectively. Therefore, the crude polysaccharides extracted from the fruiting body of H. erinaceus could improve antitumor activities in mice.

• The Korean Journal of Mycology
• /
• v.36 no.1
• /
• pp.84-92
• /
• 2008

Russula rosacea, one of edible and medicinal mushroom belonging Agaricales of Basidiomycota, has been known to have good inhibitory effects on sarcoma 180 and Ehrlich carcinoma of mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180, HepG2, HT-29 and NIH3T3 at the concentration of $10{\sim}2000\;{\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of $21.4{\sim}45%$ in mice previously inoculated with Sarcoma 180. Fr. HW improved the immuno-potentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by 6.8 fold compared with control at the concentration of $500\;{\mu}g/ml$. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 6 and 2.6 folds at the concentration of 50 mg/kg, respectively. Therefore, the antitumor effect against mice Sarcoma 180 by Russula rosacea could be due to immunomodulating activity.