• Horticultural Science & Technology
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• v.35 no.1
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• pp.131-141
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• 2017

This study was conducted to investigate in vitro mass propagation methods suitable for each growth stage of A. aristata (G. Forst.) Tindale, from spore germination to sporophyte formation. Among spores germinated in $1/8-1{\times}MS$ medium and Knop medium, Knop medium yielded the highest germination percentage (87.1%). We cultured prothalli obtained from germinating spores for 8 weeks on media with different concentrations of sucrose and active carbon, as well as different concentrations and ratios of nitrogen, to select a suitable growth medium. A. aristata (G. Forst.) Tindale prothalli grew most actively in MS medium with 3% sucrose and 20 : 40 mM of $NH_4Cl$ and $KNO_3$ (total concentration of 60 mM). We investigated sporophyte formation according to soil type, finding that bedding soil mixed with perlite at a 2 : 1(v / v) ratio yielded the highest number of sporophytes per pot ($73.8/7.5{\times}7.5cm\;pot$). By contrast, when peat moss was used alone or mixed with other substrates, prothallus development and sporophyte formation were suppressed. Therefore, the most effective propagation method for A. aristata (G. Forst.) Tindale is to grow prothalli in MS medium and to induce sporophyte formation in a mixture of bedding soil and perlite (v / v = 2 : 1).

• The Korea Journal of Herbology
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• v.24 no.2
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• pp.57-65
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• 2009

Objectives : This study was aimed to developing Korean herbalogy of the plants belonging to Aspidiaceae in Korea. Methods : The herbological books and papers published at home and abroad were researched. The total list of Aspidiaceae was made and Medicinal plants in Aspidiaceae was classified. The medicinal properties, action and applications of medicinal plants were investigated. Results : 1. There are totaled to 17 genera and 130 species in Aspidiaceae in Korea and among them medicinal plants are 11 genera, 31 species, some 24% in total. 2. Athyrium genus is main kind enough that it has 32 species among 130 species in Aspidiaceae, and medicinal plants of Dryopteris is 4 species. 3. The rhizome is the main medicinal parts if medicinal plants in the Aspidiaceae, which is used in 24 species. 4. According to nature and flavor of medicinal plants in the Aspidiaceae, they were classified into cool 15 and cold 8 species ; bitter taste 19, and little bitter taste 15 in the order. 5. According to meridian propism of medicinal plants in the Aspidiaceae, they were classified into liver meridian 5 species, stomach meridian 3 species. 6. According to the properties and principal curative action, they were classified into drugs for clearing away heat 25 species, drugs for detoxification 22 species, drugs for promoting diuresis 16 species in the order. 7. The number of toxic species in the Aspidiaceae was examined to be 5 species. Conclusions : There are totaled to 17 genera and 130 species in Aspidiaceae in Korea and among them medicinal plants are 11 genera, 31 species, some 24% in total. They were classified into drugs for clearing away heat, drugs for detoxification, drugs for promoting diuresisr.

• Korean Journal of Medicinal Crop Science
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• v.21 no.4
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• pp.301-305
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• 2013

This study was performed to select adequate plant materials for developing a natural ${\alpha}$-glucosidase inhibitor by analyzing ${\alpha}$-glucosidase inhibition activity in fronds and rhizomes of three Dryopteridaceae species: Cyrtomium fortunei, Polystichum polyblepharum, and P. lepidocaulon. The highest ${\alpha}$-glucosidase inhibitor obtained from frond of P. lepidocaulon ($4.16{\mu}g{\cdot}mL^{-1}$), and rhizome of C. fortunei ($1.84{\mu}g{\cdot}mL^{-1}$), showed much higher inhibition activity than acarbose ($1413.70{\mu}g{\cdot}mL^{-1}$). The biomass required to inhibit ${\alpha}$-glucosidase by 50% was 0.04 ~ 0.35mg for frond and 0.03 ~ 0.10mg for rhizome, and P. lepidocaulon required the least amount of fronds and P. lepidocaulon the least rhizomes. In frond, ${\alpha}$-glucosidase inhibition activity was the highest in water fraction of C. fortunei ($20.2{\mu}g{\cdot}mL^{-1}$), and n-butanol fraction of P. lepidocaulon ($9.33{\mu}g{\cdot}mL^{-1}$) and P. polyblepharum ($5.10{\mu}g{\cdot}mL^{-1}$). In rhizome, it was the highest in n-butanol fractions of C. fortunei ($19.76{\mu}g{\cdot}mL^{-1}$) and P. polyblepharum ($4.47{\mu}g{\cdot}mL^{-1}$), and ethylacetate fraction of P. lepidocaulon ($5.46{\mu}g{\cdot}mL^{-1}$). The frond biomass required for 50% ${\alpha}$-glucosidase inhibition was the lowest in the water fraction of C. fortunei (1.43mg), and n-butanol fractions of P. lepidocaulon (1.10mg) and P. polyblepharum (0.66mg). The required biomass of rhizome was the lowest in the water fraction of C. fortunei (1.59mg), and n-hexane fractions of P. lepidocaulon (0.04mg) and P. polyblepharum (0.15mg). The result of this study suggested that the three Dryopteridaceae species had high ${\alpha}$-glucosidase inhibition activity with small biomass, which might have high value as materials for economical anti-diabetic medication.