• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.1158-1159
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• 2008

본 논문에서는 상온상태에서 2W급 직접 메탄올 연료전지 모듈을 구동하여 연료전지 모듈의 성능 특성을 분석하고자 한다. 상온상태에서 연료극으로 공급하는 메탄올의 농도와 유량을 변화를 시켜 상온에서 최적의 메탄올 농도와 유량을 찾고 공기극에 공기를 공급 하였을 때 와 공기를 공급하지 않았을 때 의 연료전지의 출력을 전자 부하기를 이용하여 부하를 주고 메탄올의 유량을 변화 시켜가면서 비교해보았다.

• KSBB Journal
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• v.5 no.3
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• pp.269-277
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• 1990

Methylobacillus sp. SK1, an obligate methylotroph, was cultivated in a fed-batch culture using DO as a methanol feeding indicator with a micro computer-aided control system. While 2.07g/l of cell density was obtained after 13 hr in the batch culture (initial methanol concentration: 1.0%(v/v)),45.3g/l of cell density was obtained after 17 hr by feeding methanol and metal ions in the fed-batch culture with oxygen supply. The high-density biomass was obtained in short cultuivation time by fed-batch culture with feedback control, and consequently the biomass productivity was significantly increased. It was mainly due to extension of logarithmic growth period by methanol feeding without methanol inhibition and intensive aeration without DO limitation with microcomputer-aided control system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.5
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• pp.955-961
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• 2009

Present, a portable battery have problem that the volume increases according to capacity Increase. Direct Methanol Fuel Cell is alternative by solution plan of this problem. In this paper, the characteristics of DMFC are analyzed by change in concentration and discharge of fuel in natural convection and room temperature condition. According to the analysis result, polarization by delay of diffusion velocity of hydrogen ion appeared in methanol of low concentration. And if have a lot of supplies of methanol, generation power declines by electric cell cooling effect.

• KSBB Journal
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• v.5 no.3
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• pp.307-313
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• 1990

A microcomputer-aided fermentation system was constructed for high density fed-batch culture using dissolved oxygen(DO) as a substrate feeding indicator. DO signal was processed prior to aquisition to computer. Agitation speed and oxygen flow rate was changed stepwisely to maintain DO value at a constant level. Agitation speed was controlled by the output signal of D/A converter. Oxygen flow rate was controlled by a flow rate control valve connected to a stepping motor. Substrate was fed with a feeding pump operated by the abrupt increase of DO signal. Methylobacillus sp. SK1 was cultivated to test the system and 16.53g/l of cell density was obtained after 10 hr.

• Journal of Hydrogen and New Energy
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• v.24 no.6
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• pp.495-509
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• 2013

In this study, we develop a one-dimensional (1-D), two-phase, transient-thermal DMFC model to investigate the effect of methanol concentration fluctuation that usually occurs in active-type direct methanol fuel cell (DMFC) systems. 1-D transient simulations are conducted and time-dependent behaviors of DMFCs are analyzed under various DMFC operating conditions such as anode/cathode stoichiometry, cell temperature, and cathode inlet humidification. The simulation results indicate that the effect of methanol concentration fluctuation on DMFC performance can be mitigated by proper control of anode/cathode stoichiometry, providing a guideline to optimize operating conditions of active DMFC systems.

• KSBB Journal
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• v.15 no.2
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• pp.174-180
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• 2000

Pichia pastoris and Hansenula polymorpha, the methylotrophic yeasts have been widely used as a host for the production of e eudaryotic proteins due to the advantages related to their inherited characters. This paper describes the method to enhance t the productivity of recombinant proteins by P. pastoris and H. po$\psi$morpha. In the production of recombinant proteins using a f fed batch fermentation system, the effects of specific growth rate on the specific expression rate of re$\infty$mbinant proteins w were studied. In both species, the expression system of recombinant proteins using the fed batch fermentation was optimezed.

• Microbiology and Biotechnology Letters
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• v.18 no.3
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• pp.273-279
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• 1990

For poly- $\beta$ -hydroxybutyrate (PHB) production, a pink-pigmented facultative methylotrophic bacterium (PPFM) P-10 was newly isolated from soils through methanol-enrichment culture. The optimal medium composition for cell growth was 1.0% (vlv) of methanol as carbon source and l.Og/l of ,TEX>$NH_4Cl$, equivalent to C/N ratio of 13.2 at pH 7.0 and $30^{\circ}C$. To investigate the optimal condition for YHB accumulation, two-stage culture technique was adopted; first stage for cell growth and second stage for accumulation of PHB providing unbalanced growth conditions. The optimal PHB accumulation was 1.0% (vIv) of methanol and 0.26gll of $NH_4Cl$, C/N of 50.8 at pH 6.0. To overcome methanol inhibition on cell growth, intermittent feeding fed-batch culture technique was employed, and the cell concentration as high as 14gll with 40% of PHB was achieved. The purified PHB was identified using IR and $1^H NMR$ as homopolymer of 8hydroxybutyric acid. The absorption spectrum of extracted pink colored microbial pigment was alsa investigated.

• Korean Journal of Microbiology
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• v.46 no.3
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• pp.286-290
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• 2010

C-1 compounds are observed in anaerobic sediment of high salt environments. Thus, surface sediments and waters from these environments are therefore potential habitats for aerobic methylotrophic microorganisms. The soil samples collected from saltern and tidal flat as inoculums and methanol as carbon and energy source was supplied. After subculture depending on the salt concentration, methanol oxidizing bacteria growth condition investigated, the results of methanol oxidizing bacteria can grow in salt conditions, and the maximum concentration was 20%. Analysis based on denaturing gradient gel electrophoresis of 16S rRNA genes indicates that Methelyophaga-like bacteria were dominants of methylotrophs in the enrichment culture. Quantitative PCR showed that archaeal cells were about 1-10% of bacterial cells. Additionally archaea were assumed not to be involved in methanol oxidation since bacterial antibiotics completely blocked the methanol oxidation. Our results suggest that Methelyophaga-like bacteria could be involved in C-1 compounds oxidation in hypersaline environments although those activities are sensitive to salinity above 20%.

• Journal of Energy Engineering
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• v.2 no.2
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• pp.200-207
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• 1993

A methanol reformer was designed and fabricated using a CuO-ZnO low temperature shift catalyst, and its operation characteristics have been studied for the phosphoric acid fuel cell (PAFC) power generation system. The type of reactor was annular Methanol was consumed both for heating and for reforming fuel. Contents of carbon monoxide produced from the reformer increased as the reaction temperatures increased, but decreased as the mole ratios of water to methanol(H$_2$O/CH$_3$OH) increased. At steady state operating conditional, temperature profile of the catalytic reactor of the reformer was well coincide with the model equation, and it took 50 minutes from start to the rated condition of the reformer. When the system was operated at 4/4 and 1/4 of load, thermal efficiencies of the system were 72.3% and 77%, respectively. When the PAFC system was operated with reformed gas in the range of 62 V-37.6 V and 0-147 A, the trend of I-V curve showed a typical fuel tell characteristic. At steady state condition, the flow rates of reforming and combustion methanol were 88.1 mol/h and 50.1 mol/h, respectively.

• KSBB Journal
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• v.16 no.1
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• pp.66-70
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• 2001

Factors affecting the supercritical carbon dioxide extraction of camptothecin(CPT) and 10-hydroxycamptothecin(HCPT) from the dried powder of Cmptotheca acuminata were studied. Only a few amount of CPT and HCPT was extracted with pure supercritical carbon dioxide. Methanol and ethanol were efficient modifiers to extract CPT and HCPT. At $40^{\circ}C$, 250 bar, 1 mL/min flow rate, 41% of CPT and 35% of HCPT were extracted with supercritical carbon dioxide modified with 16% of methanol. The diffusion effect of HCPT on extraction efficiency was studied in this solid-fluid system. Round matrix hot-ball model assumption revealed that the value of D/$r^2$ was 0.0072 $min^{-1}$ which was higher than that of solvent extraction with methanol.