• Title/Summary/Keyword: 로버트

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Analysis of quaternary structure of leucine-responsive regulatory protein (Lrp) by crosslink experiments (교차결합 실험을 통한 루신 대응 조절 단백질의 4차 구조 분석)

  • Lee, Euiho;Pokoo, Robert;Nguyen, Loi Thuan;Lee, Chan Yong
    • Korean Journal of Microbiology
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    • v.53 no.4
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    • pp.297-303
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    • 2017
  • Leucine-responsive regulatory protein (LRP) is a regulatory protein of molecular weight 18.8 kDa and is widely known to regulate many metabolic and functional activities of operons in Escherichia coli. The gene for Lrp from Escherichia coli in pQE system of 6 ${\times}$ His-tagging was expressed and $^3H$-labeled protein, as well as the wild type Lrp, was purified. The crosslink experiments were performed to analyze the quaternary structure of Lrp at high of $5{\mu}M$ and at low concentrations below $0.3{\mu}M$ with cross linkers, such as glutaraldehyde, 1, 2, 3, 4-diepoxy-butane (DEB), and ethylene glycol bis (succinimidyl succinate) (EGS). In the experiments, we found that the Lrp protein can be formed higher conformation states of tetramer, hexamer, octamer, as well as dimeric state when incubated with the above cross linkers.

On the immanent Problems of Liberalism and Hegels Philosophy of Right (자유주의의 내재적 문제와 헤겔의 법철학)

  • Kwon, Young-woo
    • Journal of Korean Philosophical Society
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    • v.147
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    • pp.29-58
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    • 2018
  • The purpose of this article is to demonstrate that Hegel's philosophy of right is a dialectic critique of liberalism. The dialectical criticism in this article does not mean the formal logical denial, but a return to self by self-negation. Thus, if Hegel's philosophy of right is a dialectical critique of liberalism, Hegel's philosophy of right will be critical of liberalism and at the same time, it will not reject liberalism, but rather have aspects of liberalism. The criticism of liberalism implies that individual freedoms and rights can not be realized subjectively through individual free acts, but are realized intersubjectively through social mediums. And this is also found in controversies among modern liberalists because modern liberalism requires the government's role and institutional arrangements for the realization of individual freedoms and rights. We can find the aspects of liberalism in Hegel's philosophy of right since Hegel's ethical life entails ultimately the concrete realization and extension of individual freedom and rights.

Olafur Eliasson and the Reuturn of Medieval Aesthetics (올라퍼 엘리아슨과 중세미학의 귀환)

  • Chin, Jungkwon
    • The Journal of the Korea Contents Association
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    • v.19 no.7
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    • pp.220-233
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    • 2019
  • Hitherto there have been by and large three different approaches to the installation works of Olafur Eliasson; a soma-aethetic, politico-aesthetic and techno-aesthetic. But none of these provides us with the aesthetic descriptions of the light effect. This failure seems to arise from the lack of the conceptual tools suit for describing the atmospheric effect of 'light'. The symbolism of light, or the theological optics of Middle Age may help us to compensate for the lack of appropriate concepts needed for theorizing the effect of light used very frequently by contemporary installation artists. And this medieval aesthetics of light can also of service to elucidate some essential characteristics of the digtal visual culture in general.

Film: Ending of a Story and an Open Ending (영화, 이야기의 결말과 열린 결말)

  • Kim, Sam-Ryeok
    • The Journal of the Korea Contents Association
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    • v.19 no.7
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    • pp.47-53
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    • 2019
  • This study is an analysis of endings of films and open endings. Particularly, the key of this study is the investigation of the definition and interpretation of open endings. For this purpose, the 'ending' was defined first, and subsequently, endings were divided into 'closed' and 'open' endings with a goal pursuit model of David Howard and Robert McKee. Thus, it was found in this study that the thoughts of an audience as well as the intention of a creator are crucial in defining an ending. Although a story is designed by a creator, its interpretation is at the disposal of an audience in the end, and the audience may take an ending differently depending on their value system or interest in the story, which eventually leads to their own definition of the ending. For sure, a traditional fact is that most of films including those mentioned to have open endings have closed endings, and such a structure of stories will not change significantly in the future. However, the ending of a film designed to have a closed ending may be changed to be open ended depending on the intention of a creator or the thoughts of an audience, and furthermore, the intention of an open ending may not be readily accepted by an audience.

Ameliorative Effects of NXP031 on MPTP-Induced Neurotoxicity (MPTP로 유도된 신경 독성에 대한 NXP031의 개선 효과)

  • Lee, Joo Hee;Song, Min Kyung;Kim, Youn-Jung
    • Journal of Korean Biological Nursing Science
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    • v.23 no.3
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    • pp.199-207
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    • 2021
  • Purpose: The purpose of this study was to investigate effects of NXP031, an inhibitor of oxidation by specifically binding to the complex of DNA aptamer/vitamin C, on dopaminergic neurons loss and the reaction of microglia in an animal model of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced subchronic Parkinson's disease (PD). Methods: A subchronic PD mouse model was induced via an intraperitoneal (IP) injection of MPTP 30 mg/kg per day for five days. NXP031 (vitamin C/aptamer at 200 mg/4 mg/kg) and vitamin C at 200 mg/kg were administered via IP injections at one hour after performing MPTP injection. This process was performed for five days. Motor function was then evaluated with pole and rotarod tests, after which an immunohistochemical analysis was performed. Results: NXP031 administration after MPTP injection significantly improved motor functions (via both pole and rotarod tests) compared to the control (MPTP injection only) (p<.001). NXP031 alleviated the loss of dopaminergic neurons in the substantia nigra (SN) and striatum caused by MPTP injection. It was found to have a neuroprotective effect by reducing microglia activity. Conclusion: NXP031 can improve impaired motor function, showing neuroprotective effects on dopaminergic neurons in the SN and striatum of MPTP-induced subchronic Parkinson's disease mouse model. Results of this study suggest that NXP031 has potential in future treatments for PD and interventions for nerve recovery.

Reading Elizabeth Bishop in Her Relationship with Moore and Lowell: Looking into the "Intrinsic Qualities" of Bishop's Poetry (무어, 로월과의 관계 속에서 엘리자베스 비숍 읽기 -비숍 시의 "내적 특성" 들여다보기)

  • Kim, Yangsoon
    • Journal of English Language & Literature
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    • v.55 no.1
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    • pp.25-59
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    • 2009
  • This study explores the characteristics of Elizabeth Bishop's poetry in comparison with the two of her closest friends and poets, Marianne Moore and Robert Lowell. Bishop's reputation has dramatically changed since her death. In the 1970s she was "a writer's writer's writer," and admired by a small group of poets or critics. Since 1990s, however, there has been a great shift in the evaluation of her poetry, which is so called "The Elizabeth Bishop Phenomenon." It does not seem to be an easy task to examine what has driven the phenomenon, and why she used to be a minor poet or "the most honored yet most elusive of poets" but now she has a widespread recognition by the academy and beyond it. The "intrinsic qualities" of Bishop's poetry, however, can be one of the main reasons why it took several decades for Bishop to become a central figure in the literary canon. Looking into her "intrinsic qualities," this paper discusses Bishop's "The Fish," "Roosters" through the Moore-Bishop relationship, and reads Bishop's "Armadillo" and "The Monument" through the Lowell-Bishop relationship. It also deals with letters, interviews, Moore's "The Fish," and Lowell's "Skunk Hour" and "For the Union Dead" to show the Bishop's deep and complex relationships with the two poets, and more importantly their differences. Bishop's poetry is difficult, "elusive," and sometimes "enigmatic," not because her texts are full of difficult words to understand but because there are the subtle interchange between perception and meaning, "the dynamics of keen feeling," the unresolved patterns, and the transient vision under the seemingly transparent surface of the texts.

American Culture at the Crossroad : Debates over NEA(National Endowments for the Arts) (미국 문화, 그 기로에 서서 - NEA(국립예술진흥기금)를 둘러싼 논쟁 중심으로)

  • Kim, Jin-A
    • The Journal of Art Theory & Practice
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    • no.4
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    • pp.33-56
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    • 2006
  • The cultural debates between conservatives and liberals at the end of the 1980s and in the early 1990s were termed as "culture wars." The "culture wars" involved a diverse range of controversial issues, such as the introduction of multicultural curricula in educational institutions, prayers in schools, whether to allow gays to serve openly in the military, and whether abortion should be permitted. The most heated debates of the "culture wars" regarding art raged over the NEA and the question of whether Andres Serrano's works should have been publicly funded, in addition to the exhibition "Robert Mapplethorpe: The Perfect Moment" which were charged as projecting "obscene" or "blasphemous" images. This paper examines the development of culture wars in art and focuses on several issues invoked by the NEA debates. However, it is not a detailed chronological investigation. Rather it pays attention to the several phases of the debates, analyzing and criticizing the clashes of the political and esthetical points of views between conservatives and liberals. How could NEA funding, a mere fraction of the federal budget, have become so critical for both sides(conservative and liberal), for politicians and artists' groups, and for academics and the general public? The art community was astounded by this chain of events; artists personally reviled, exhibitions withdrawn and under attack, the NEA budget threatened, all because of a few images. For conservative politicians, the NEA debate was not only a battle over the public funding of art, but a war over a larger social agenda, a war for "American values and cultures"based on the family, Christianity, the English language, and patriarchy. Conservative politicians argued the question was not one of "censorship" but of "sponsorship," since the NEA charter committed it to "helping museums better serve the citizens of the United States."Liberals and art communities argued that the attempt to restrict NEA funding violated the First Amendment rights of artists, namely "free speeches." "No matter how divided individuals are on matters of taste," Arthur C. Danto wrote, "freedom is in the interest of every citizen." The interesting phase is that both sides are actually borrowing one another's point of view when they are accompanied by art criticism. Kramer, representative of conservative art critic, objected the invasion of political contents or values in art, and struggled to keep art's own realm by promoting pure aesthetic values such as quality and beauty. But, when he talked about Mapplethorpe's works, he advocated political and ethical values. By contrast, art experts who argued for Mapplethorpe's works in the Cincinnati trial defended his work, ironically by ignoring its manifest sexual metaphor or content although they believed that the issues of AIDS and homosexuality in his work were to be freely expressed in the art form. They adopted a formalistic approach, for example, by comparing a child nude with putti, a traditional child-angel icon. For a while, NEA debates made art institutions, whether consciously or unconsciously, exert self-censorship, yet at the same time they were also producing positive aspects. To the majority of people, art was still regarded as belonging to the pure aesthetic realm away from political, economical, and social ones. These debates, however, were expanding the very perspective on the notion of what is art and of how art is produced, raising questions on art appreciation, representation, and power. The interesting fact remains: had the works not been swiped in NEA debates, could the Serrano's or Mapplethorpe's images gain the extent of power and acceptance that it has today?

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Construction, Expression, and Purification of N-Terminal Variants of Lumazine Protein from Photobacterium leiognathi (발광세균 Photobacterium leiognathi의 돌연변이 아미노-말단 루마진 단백질들의 제조, 발현 및 정제)

  • Kang, Kyoung-Suk;Kim, So-Young;Choi, Ji-Sun;Kim, Young-Doo;Pokoo, Robert;Nam, Ki-Seok;Lee, Chan Yong
    • Korean Journal of Microbiology
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    • v.49 no.2
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    • pp.205-210
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    • 2013
  • Lumazine protein is a fluorescent protein isolated from the bioluminescent bacteria of Photobacterium species. To generate minimal size of lumazine protein with possessing fluorescent characteristic, the gene coding for the wild type N-terminal domain of lumazine protein (N-LumP 118) containing amino acids up to 118 from Photobacterium leiognathi was produced. In addition, the genes coding for the variant proteins of N-LumP 118, replaced with one tryptophan amino acid (N-LumP 118 V41W, S48W, T50W, D64W, and A66W), were also constructed by Polymerase Chain Reaction and Site Directed Mutagenesis. These proteins were expressed in Escherichia coli by transformation with recombinant plasmids and purified by 6X-His tagging system. Spectroscopic studies have show that the purified proteins are capable of binding to the fluorescent ligand 6,7-dimethyl-8-ribityllumazine, resulted in showing of fluorescent characteristic with the minimal size of protein. From these studies, the mutant proteins containing single tryptophan amino acid residue, possessing its own intrinsic flouophore character at the different position, will be able to the use as a probe for further studies to deduce their three dimensional structure and the binding modes.

A study on the history of Idealistic Rural Community Movement -From the beginning until 1945- (이상농촌운동의 역사 소고 -시작에서 1945년 이전까지-)

  • Lim, Kwang Myung
    • Journal of Agricultural Extension & Community Development
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    • v.21 no.2
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    • pp.101-141
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    • 2014
  • Idealistic Rural Community Movement(Rural Utopia Movement) was one of the most powerful rural community movements in the world. Regardless of the East or the West, people started dreaming of happiness through idealization of rural communities as early as several centuries ago and have tried to implement this as a social movement to make the dream come true. Western rural utopia movement started when Thomas More published "Utopia" in England in 1516 and spread to many other countries in various forms. Chinese version of rural utopia was well portrayed in both "So Gook Gwa Min" (small population in a small country) written by Lao-tzu in the 6th century B.C. and "Arcadia" by Do, Yun-Myoung in the $4^{th}$ century. In Korea, the rural utopia people pursued was vividly described in the following three. First, Yul Do Gook portrayed in "Hong Gil Dong Jun" written by Hu Gyoon. Second, Gong Do portrayed in "Hu Sang Jun" written by Park Ji Won. Third, Sin Min Hwe's rural utopia movement made in the entire nation as well as Manchuria and America so as to save the country, which contributed to protecting the rural communities and arousing hopes in farmers by making a good model. This movement is especially important in that it became the foundation of modernization of Korea through Ga Na An(Canaan) Farmers' School and Sae Ma Ul Woon Dong(New village movement), which were heavily influenced by continued Sin Min Hwe's rural utopia movement.

A Study on the Design Management & Future Design Strategy of Philips (Philips사의 디자인경영 및 미래디자인 전략에 대한 연구)

  • 이해묵
    • Archives of design research
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    • v.13 no.4
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    • pp.85-93
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    • 2000
  • Design becomes a source of new competitive power in the boundless global market so-called globalization. The competitive power in business was lied in the technology in 70's and the design was understood as a styling or graphic means. However, the design has become more important means to get the competitive power in business since 1980. World businesses have found the fact that it has a super competitive power to make the product's performance as well as its dignity rather than it is to determine the product's external view or color. The change of design policy in Phillips, one of the world's leading producers of electronic products, is not much different. Design manager's power was limited until 70's. However, Phillips has focused its business strategy on the higher competitive power since 1980 and they welcomed Robert Blaich, vice president of design and development at Herman Miller Inc., to be a member of the company, expanding the importance of design along with restructure while working on the globalization. Meanwhile, Stefano Marzano, a Senior Director in 90's, established a high design concept, working on the strategic futures to get customer-oriented and for successful commercialization. The vision of the future developed over 3 years until 1996 was to forecast 10 years coming up and create a new value while achieving the business target through the design as an innovative design in bracing for the information network era.

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