• Journal of the Korean Society for Nondestructive Testing
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• v.31 no.5
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• pp.522-528
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• 2011

Artificial basilar membranes made of PVDF(polyvinylidene fluoride) are manufactured using microfabrication processes. The mechanical behavior of PVDF artificial basilar membrane was measured to evaluate its performance as a mechanical frequency analyzer using scanning LDV(laser Doppler vibrometer). The experimental setup consists of the microfabricated artificial basilar membrane, a loud speaker connected to an amplifier for generating acoustic pressure of specific spectral pattern, and a scanning LDV with controlling unit for measuring the displacement of the membrane on the incoming acoustic stimulation. The microfabricated artificial basilar membrane was attached tightly upon a package containing a chamber which can be filled with silicone oil before placed on the experimental setup stage. The experiment results showed that the microfabricated artificial basilar membrane has a property as a mechanical frequency analyzer.

• 한국연소학회:학술대회논문집
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• 2005.10a
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• pp.170-178
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• 2005

Multi-hole type oxygen combustion burner was developed for industrial gasification and smelting furnace. We investigated characteristics of flame, radiation transfer, and soot emission in the convectional oxygen burner with respect to the feeding condition of fuel and oxygen. Regarding the results of the conventional burner, we designed new burners which have larger fuel consumption rate and radiation heat transfer. We changed the size and hole number and shape of the exit plane of the burner. In addition, the performance of the burner was tested with respect to the feeding condition of the fuel and air: Normal Diffusion flame(NDF) and Inverse Diffusion Flame(IDF). We investigated the flame configuration, radiation heat transfer, and soot formation by using a CCD camera, heat flux meter, and Laser Induced Incadescence(LII), respectively. The stable operating condition was obtained by the flame configuration and the flame of the burner which has dented exit plane was more stable in whole operating conditions. The characteristics of radiative heat transfer were sensitive to the feeding condition of reactants and the flame of 75% primary oxygen and 25% secondary oxygen of the IDF case shows maximum radiation heat transfer. The soot volume fraction of the flame was measured in the axial direction of the flame and the amount of soot volume fraction is proportion to the radiation heat transfer. As a result, we can get the optimal operating condition of the newly designed burner which enhances the characteristics of flame stabilization and radiation heat transfer.

• Journal of the Korea Computer Industry Society
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• v.2 no.3
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• pp.363-368
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• 2001

This paper introduced cascading technique as a new technology composed of two pulse transformers and presented the experimental data and results. To obtain the stable pulse voltage adopted cascading technique, we designed and tested a compact pulse generator by adjusting the load resistors and input voltage. Adopting cascading technique to load, we found that average cascading voltage was about 62$\%$ of theoretical value. Cascading ratio was calculated at almost 19 compared with non cascading voltage.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.161-161
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• 2014

열플라즈마는 주로 아크 방전에 의해 발생시킨 전자, 이온, 중성입자(원자 및 분자)로 구성된 부분 이온화된 기체로, 국소열평형상태를 유지하여 구성입자가 모두 수천에서 수만도에 이르는 같은 온도를 갖는 고속의 제트 화염 형태를 이루고 있다. 이렇게 고온, 고열용량, 고속, 다량의 활성입자를 갖는 열플라즈마의 특성을 이용하여, 종래 기술에서는 얻을 수 없는 다양하고 효율적인 산업적 이용이 활발히 진행되고 있다. 용사코팅은 노즐 출구를 통해서 외부로 방출되는 열 플라즈마 화염을 이용하는 것으로 이 화염의 와류 특성으로 인하여 외기의 가스가 화염내부로 침투하는 특성을 가진다. 이러한 현상은 열원의 냉각효과 외에도 외기를 구성하는 기체 분자의 내부 유입을 의미하는 것으로 대기 상태에서 공정이 이루어진다면 열원 내로 유입되는 대기 내의 산소가 모재 표면과 반응하여 산화가 진행된다. 이러한 산화과정은 용사 코팅의 품질을 저하시키는 요인이 되므로, W, Ti 등과 같은 반응성이 높은 재료의 코팅은 산화과정을 방지하기 위하여 진공에서 코팅을 하여야만 한다. 진공 플라즈마용사코팅은 진공 또는 저압의 불활성 분위기 중에서 열플라즈마 화염에 용사재료를 투입하여 플라즈마 화염 내부에서 순간적으로 이를 용융시킨 후 고속으로 분출, 모재에 적층시키는 코팅공정이다. 이때 분말상의 용사재료를 고속으로 화염 중심에 투입하여 최대 에너지 전달이 이루어지도록 하는 것이 적층효율 및 코팅품질을 향상에 필수적이다. 하지만 플라즈마 화염 내부를 고속으로 이동하는 입자의 온도와 속도 및 궤적을 측정하여 제어하는 것은 매우 어렵기 때문에, 통상 형성된 코팅의 구조와 두께로부터 경험적으로 파라미터를 결정하는 것이 일반적이다. 본 연구에서는 초고속 레이저 카메라와 이미지 분석용 소프트웨어를 이용하여 플라즈마 화염내의 비행입자 궤적을 추적하고, 이를 통해 분말 이송가스의 유량이 코팅 효율 및 미세구조에 미치는 영향을 조사하였다. 플라즈마 화염은 중심부가 가장 높은 온도와 속도를 가지고 있기 때문에, 분말 이송가스의 유량이 적을 경우 투입된 분말은 단지 플라즈마 화염의 상부 경계면을 지나는 궤적을 갖게된다. 이로 인해 분말의 용융이 충분히 이루어지지 않아 적층 효율이 낮고 미용융 입자 및 기공이 많은 미세구조를 보였다. 이송가스 유량을 증가시키게 되면, 분말의 궤적은 플라즈마 화염의 중심부를 지나게 되어 적층 효율이 증가하고 미세구조 또한 개선되었다. 하지만 이송가스 유량이 지나치게 클 경우, 투입된 분말 입자는 플라즈마 화염을 조기에 관통하게 되어 비행궤적은 온도와 속도가 낮은 영역에 형성되었다.

• Korean Journal of Optics and Photonics
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• v.16 no.1
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• pp.50-55
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• 2005

A method for measuring the wavefront error and the modulation transfer function(MTF) of large aperture optics using an unequal path interferometer is presented. A bidirectional shearing interferometer is used for collimation testing of the measurement system. A large aperture Fizeau interferometer with long optical path difference measures the wavefront error of the optics under test by using a $\Phi$ 400 mm off-axis parabolic mirror. The MTF is also measured at the wavelength of the interferometer by changing the laser light into partially incoherent light. Test results of a $\Phi$ 300 mm Cassegrain type satellite telescope made in Korea are presented.

• Applied Microscopy
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• v.35 no.3
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• pp.121-128
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• 2005

It has been known that ras signaling transduction leads to cell proliferation and migration including various adaptor molecules. Dynamin protein has been implicated in the formation of nascent vesicles in both the endocytic and secretory pathways. Dynamin was classified into three isoforms: dynamin I is only expressed in neuronal tissue, dynamin II is expressed ubiquitously in all tissue but that of dynamin III is confined to testis. We have reported in previous study that Grb2, binding to ras, was associated with dynamin II in NIH3T3 cells. Therefore we have tried to identify the relative expression of dynamin II according to overexpressed ras protein in ras oncogene transfected cells (NIH3T3 (ras)). For the detection of differential expression of dynamin II, we have used immunofluorescent staining and western blot methods in NIH3T3 and NIH3T3 (ras) cells. Next we have described the morphological differences between NIH3T3 and NIH3T3 (ras) cells using SEM and TEM. From these experiments dynamin II was highly expressed in NIH3T3 (ras) cells. NIH3T3 cells was transformed to more spindle shape with many cell process by transfection of ras oncogene. Moreover dynamin II was more concentrated in endocytotic membrane of the NIH3T3 (ras) cells compared to that of NIH3T3 cells. The present results suggested that dynamin II may involve the intermediate messenger in Ras signaling transduction pathway.

• Journal of Life Science
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• v.25 no.8
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• pp.953-959
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• 2015

Optogenetics is the combination of optical and molecular strategies to control designated molecular and cellular activities in living tissues and cells using genetically encoded light-sensitive proteins. It involves the use of light to rapidly gate the membrane channels that allows for ion movement. Optogenetics began with the placing of light-sensitive proteins from green algae inside specific types of brain cells. The cells can then be turned on or off with pulses of blue and yellow light. Using the naturally occurring algal protein Channelrhodopsin-2 (ChR2), a rapidly gated light-sensitive cation channel, the number and frequency of action potentials can be controlled. The ChR2 provides a way to manipulate a single type of neuron while affecting no others, an unprecedented specificity. This technology allows the use of light to alter neural processing at the level of single spikes and synaptic events, yielding a widely applicable tool for neuroscientists and biomedical engineers. An improbable combination of green algae, lasers, gene therapy and fiber optics made it possible to map neural circuits deep inside the brain with a precision that has never been possible before. This will help identify the causes of disorders like depression, anxiety, schizophrenia, addiction, sleep disorder, and autism. Optogenetics could improve upon existing implanted devices that are used to treat Parkinson’s disease, obsessive-compulsive disorder and other ailments with pulses of electricity. An optogenetics device could hit more specific subsets of brain cells than those devices can. Applications of optogenetic tools in nonneuronal cells are on the rise.

• Applied Chemistry for Engineering
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• v.26 no.3
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• pp.319-325
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• 2015

Submicron-sized $CeO_2:Er^{3+}/Yb^{3+}$ upconversion phosphor particles were synthesized by spray pyrolysis, and their luminescent properties were characterized by changing the concentration of $Er^{3+}$ and $Yb^{3+}$. $CeO_2:Er^{3+}/Yb^{3+}$ showed an intense green and red emission due to the $^4S_{3/2}$ or $^2H_{11/2}{\rightarrow}^4I_{15/2}$ and $^4F_{9/2}{\rightarrow}^4I_{15/2}$ transition of $Er^{3+}$ ions, respectively. In terms of the emission intensity, the optimal concentrations of Er and Yb were 1.0 % and 2.0%, respectively, and the concentration quenching was found to occur via the dipole-dipole interaction. Upconversion mechanism was discussed by using the dependency of emission intensities on pumping powers and considering the dominant depletion processes of intermediate energy levels for the red and green emission with changing the $Er^{3+}$ concentration. An energy transfer from $Yb^{3+}$ to $Er^{3+}$ in $CeO_2$ host was mainly involved in ground-state absorption (GSA), and non-radiative relaxation from $^4I_{11/2}$ to $^4I_{13/2}$ of $Er^{3+}$ was accelerated by the $Yb^{3+}$ co-doping. As a result, the $Yb^{3+}$ co-doping led to greatly enhance the upconversion intensity with increasing ratios of the red to green emission. Finally, it is revealed that the upconversion emission is achieved by two photon processes in which the linear decay dominates the depletion of intermediate energy levels for green and red emissions for $CeO_2:Er^{3+}/Yb^{3+}$ phosphor.

• 한국해양학회지
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• v.27 no.1
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• pp.19-34
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• 1992

Marine seismic refraction surveys have been carried out by Korea Institute of Geology, Mining and Materials(KIGAM) since 1984. The recording of refraction data was based on analog instrumentation. Therefore the resolution of refraction data was not good enough to distinguish many layers. The objective of the interpretation of seismic refraction data is the determination of intervals and critically refracted seismic wave propagation velocities through the layers beneath the sea floor. To determine intervals and velocities precisely, the resolution of refraction data should be enhanced. The intent of the study is to improve the quality of shallow marine refraction data by the digital technique using microcomputer- based acquisition and processing system. The system consists of an IBM AT microcomputer clone, an analog-digital(A/D) converter. A mass storage unit and a parallel processing board. The A/D converter has 12 bits of precision and 250 kHz of conversion rate. The magneto-optical disk drive is used for the mass storage of seismic refraction data. Shallow marine seismic refraction surveys have been carried out using the system at 6 locations off Ulsan and Pusan area. The refraction data were acquired by the radio sonobuoy. The refraction profiles have been produced by the laser printer with 300 dpi resolution after the basic computer processing. 5-9 layers were interpreted from digital refraction profiles, whereas 2-4 layers were interpreted from analog refraction profiles. the propagation velocities of sediments were interpreted as 1.6-2.1 km/sec. The propagation velocities of acoustic basement were interpreted as 2.4-2.7 km/sec off Ulsan area, 4.8 km/sec off Pusan area.

• KSBB Journal
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• v.20 no.4
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• pp.323-327
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• 2005

RT-PCR is an useful method to investigate the expression of target gene as detection tools. Although RT-PCR is the powerful detection method for tissues, it was difficult to amplify the target gene product using the single cell. To clarify the expression level of the genes related to Parkinson's disease (PD), I performed the laser dissection of single cell from Substantia nigra. I examined the mRNA expression level in the dopaminergic neuron isolated from the PD patients by the single cell RT-PCR method. It is known that tyrosine hydroxylase (TH), DOPA decarboxylase (DDC) are involved in biosynthesis of the catecholamine such as dopamine. Little has been known about the gene expression features of these enzymes in single dopaminergic neuron. I could detect the specific gene products in single cell level. The different expression was observed in PD-related gene products from the single neuron of PD patients. Interestingly, TH gene expression was significantly decreased with comparing the ratio of decrease in other PD-related genes. Hence, I represented data that indicate the RT-PCR method described in this report is an effective method in detecting a specific single-cell mRNA level related with diseases.