• Title/Summary/Keyword: 레이저광

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Recovery of Streptococcus Mutans Biofilm after Photodynamic Therapy with Erythrosine and LED Light Source (Erythrosine과 LED를 이용한 광역동 치료 후 Streptococcus mutans 바이오필름의 회복)

  • Yongwook, Shin;Howon, Park;Juhyun, Lee;Siyoung, Lee
    • Journal of the korean academy of Pediatric Dentistry
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    • v.49 no.2
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    • pp.149-157
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    • 2022
  • The aim of this study was to evaluate the effects of erythrosine-mediated photodynamic therapy (PDT) on Streptococcus mutans biofilm recovery by counting its colony-forming units (CFUs) and via confocal laser scanning microscopy analysis at different time points following PDT. In PDT, photosensitizer was an erythrosine. S. mutans ATCC25175 biofilms were irradiated using an LED curing light. Chlorhexidine (CHX) was used as positive control. After each antimicrobial treatment, samples were cultured to allow biofilm recovery. Viability was measured by calculating the CFU counts after treatment and after every 3 hours for up to 24 hours. Immediately after treatment, the PDT and CHX groups showed equally significant decreases in S. mutans CFU counts compared to the negative control. After 12 hours of reculture, the PDT group showed no significant difference in the decrease in CFU count compared to the negative control, whereas the CHX group showed significantly lower CFU counts throughout the 24-hour period. Erythrosine-mediated PDT can effectively inhibit S. mutans biofilm formation. However, biofilm recovery occurred earlier in the CHX group after PDT. This study provides insights into the clinical effectiveness of PDT in preventing dental caries.

Rotational Prism Stitching Interferometer for High-resolution Surface Testing (고해상도 표면 측정을 위한 회전 프리즘 정합 간섭계)

  • In-Ung Song;Woo-Sung Kwon;Hagyong Khim;Yun-Woo Lee;Jong Ung Lee;Ho-Soon Yang
    • Korean Journal of Optics and Photonics
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    • v.34 no.3
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    • pp.117-123
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    • 2023
  • The size of an optical surface can significantly affect the performance of an optical system, and high spatial frequency errors have a greater impact. Therefore, it is crucial to measure the surface figure error with high frequency. To address this, a new method called rotational prism stitching interferometer (RPSI) is proposed in this study. The RPSI is a type of stitching interferometer that enhances spatial resolution, but it differs from conventional stitching interferometers in that it does not require the movement of either the mirror tested or the interferometer itself to obtain sub-aperture interferograms. Instead, the RPSI uses a beam expander and a rotating Dove prism to select particular sub-apertures from the entire aperture. These sub-apertures are then stitched together to obtain a full-aperture result proportional to the square of the beam expander's magnification. The RPSI's effectiveness was demonstrated by measuring a 40 mm diameter spherical mirror using a three-magnification beam expander and comparing the results with those obtained from a commercial interferometer. The RPSI achieved surface testing results with nine times higher sampling density than the interferometer alone, with a small difference of approximately 1 nm RMS.

Proof-of-principle Experimental Study of the CMA-ES Phase-control Algorithm Implemented in a Multichannel Coherent-beam-combining System (다채널 결맞음 빔결합 시스템에서 CMA-ES 위상 제어 알고리즘 구현에 관한 원리증명 실험적 연구)

  • Minsu Yeo;Hansol Kim;Yoonchan Jeong
    • Korean Journal of Optics and Photonics
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    • v.35 no.3
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    • pp.107-114
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    • 2024
  • In this study, the feasibility of using the covariance-matrix-adaptation-evolution-strategy (CMA-ES) algorithm in a multichannel coherent-beam-combining (CBC) system was experimentally verified. We constructed a multichannel CBC system utilizing a spatial light modulator (SLM) as a multichannel phase-modulator array, along with a coherent light source at 635 nm, implemented the stochastic-parallel-gradient-descent (SPGD) and CMA-ES algorithms on it, and compared their performances. In particular, we evaluated the characteristics of the CMA-ES and SPGD algorithms in the CBC system in both 16-channel rectangular and 19-channel honeycomb formats. The results of the evaluation showed that the performances of the two algorithms were similar on average, under the given conditions; However, it was verified that under the given conditions the CMA-ES algorithm was able to operate with more stable performance than the SPGD algorithm, as the former had less operational variation with the initial phase setting than the latter. It is emphasized that this study is the first proof-of-principle demonstration of the CMA-ES phase-control algorithm in a multichannel CBC system, to the best of our knowledge, and is expected to be useful for future experimental studies of the effects of additional channel-number increments, or external-phase-noise effects, in multichannel CBC systems based on the CMA-ES phase-control algorithm.

A Study on the Measurement of the Dimensionless Light Extinction Constant for Particulate Matter from Fuel Oil for Marine and Land Diesel Engines (선박 및 육상 디젤 엔진용 연료유에서 발생하는 입자상물질에 대한 무차원 광소멸계수 계측에 관한 연구)

  • Rho, Beom-Seok;Choi, Jae-Hyuk;Cho, Kwon-Hae;Park, Seul-Hyun;Lee, Won-Ju
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.24 no.2
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    • pp.275-281
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    • 2018
  • It is known that he pollutant emitted from the combustion process of marine fuel oil causes air pollution and harmful effects to the human body. Accordingly, IMO regulates pollutants emitted from ships. However, the regulation of Particulate Matter (PM) is still in the process of debate, so preemptive action is needed. Fundamental research on PM is essential. In this study, the Dimensionless Light Extinction Constant ($K_e$) of fuel oil used in marine diesel engines was measured and analyzed to construct the basic data of the PM generated from marine-based fuel oil. The fuel oil used in the land diesel engine was measured in the same way for character comparison. Both fuel oils differ in sulfur content and density. The $K_e$ was measured via the optical method using a 633 nm laser and was determined by using the volume fraction of PM collected by the gravimetric filter method. The $K_e$ of the PM discharged from marine fuel oil is 8.28, and the land fuel oil is 8.44. The $K_e$ of two fuel oils was similar within the measurement uncertainty range. However, it was found by comparison with the value obtained by the Rayleigh-Limit solution that the light scattering portion could be large. Also, it was found that light extinction characteristics could be different due to the relationship between light transmittance and collected mass.

HISTOLOGIC FEATURE AND INFILTRATION OF ADHESIVE RESIN ACCORDING TO PRETREATMENT ON PROXIMAL EARLY CARIES LESION (평활면 초기 우식병소의 표면처리에 따른 조직상 및 접착제의 침투 양상 비교)

  • Kim, In-Young;Jeong, Tae-Sung;Kim, Shin
    • Journal of the korean academy of Pediatric Dentistry
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    • v.36 no.1
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    • pp.30-37
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    • 2009
  • Early enamel caries is commonly remineralized by the patient‘s improved oral hygiene or fluoridation, however the result is clinically unreliable. As an alternative, we tried to seal the lesions with low-viscosity light-curing resin. The aim of the present study was to search the proper methods of the adequate pretreatment prior to applying adhesive resin on natural proximal caries lesions. Thirty nine extracted deciduous molar teeth showing proximal early caries lesion were used for this study. They were divided into 5 groups : Group 1; only carefully cleaned with water, group 2; etched with 15% HCl for 15s, group 3; etched with 35% phosphoric acid for 15s, group 4; etched with 35% phosphoric acid for 30s, and group 5; cleaned with 0.5% NaOCl. Following results were obtained by evaluating with SEM and CLSM after applied with adhesive resin. 1. As a result of SEM evaluation, group 2 showed clearly removed surface layer, group 3,4 showed partially removed surface layer irregularly, group 5 showed slightly removed surface layer. 2. Group 2 showed the deepest infiltration depth, followed by group 4, group 3, group 5, group 1 and besides group 5, other groups showed significantly deep infiltration depth. (p < 0.01) In conclusion, the best methods of the adequate pretreatment on natural proximal caries lesion for deep infiltration of adhesive resin was to etch with 15% HCl for 15s.

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ABRASION AND CHEMICAL DEGRADATION OF LIGHT-CURED COMPOSITE RESIN FOR UPDATED RESIN DEVELOPMENT (차세대 레진개발을 위한 광중합형 복합레진의 마모와 화학적 분해)

  • Yang, Kyu-Ho;Choi, Nam-Ki;Yook, Geun-Young
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.4
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    • pp.685-695
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    • 2004
  • The aim of this study was to evaluate the resistance to degradation and to compare the wear resistance characteristics of four composite resins in an alkaline solution. The resistance to degradation was evaluated on the basis of mass loss(%), degradation depth(${\mu}m$), Si loss(ppm) and wear depth. The brands studied were Heliomolar flow, Filtek supreme, Point4, Tetric flow. The results were as follows: 1. The sequence of the mass loss was in descending order by Heliomolar flow, Filtek supreme, Point4, Tetric flow. There was significant differences among the materials except Heliomolar flow and Filtek supreme. 2. The sequence of the degree of degradation layer depth was in descending order by Filtek supreme, Heliomolar flow, Tetric flow, Point4. There were significant differences among the materials except Heliomolar flow and Tetric flow. 3. The sequence of Si loss was in descending order by Filtek supreme, Heliomolar flow, Point4, Tetric flow. There were significant differences among the materials except Point 4 and Tetric flow. 4. The sequence of maximum wear depth was in descending order by Heliomolar flow, Point4, Fillet supreme, Tetric flow and there was increasing wear depth on soaking in 0.1N NaOH solution. 5. When observed with SEM, destruction of bonding between matrix and filler was observed and when observed with CLSM, the depth of degradation layer of specimen surface was observed. There results indicate that wear and hydrolytic degradation could be considered to be evaluation factors of composite resins.

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Observation of Methane Flux in Rice Paddies Using a Portable Gas Analyzer and an Automatic Opening/Closing Chamber (휴대용 기체분석기와 자동 개폐 챔버를 활용한 벼논에서의 메탄 플럭스 관측)

  • Sung-Won Choi;Minseok Kang;Jongho Kim;Seungwon Sohn;Sungsik Cho;Juhan Park
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.4
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    • pp.436-445
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    • 2023
  • Methane (CH4) emissions from rice paddies are mainly observed using the closed chamber method or the eddy covariance method. In this study, a new observation technique combining a portable gas analyzer (Model LI-7810, LI-COR, Inc., USA) and an automatic opening/closing chamber (Model Smart Chamber, LI-COR, Inc., USA) was introduced based on the strengths and weaknesses of the existing measurement methods. A cylindrical collar was manufactured according to the maximum growth height of rice and used as an auxiliary measurement tool. All types of measured data can be monitored in real time, and CH4 flux is also calculated simultaneously during the measurement. After the measurement is completed, all the related data can be checked using the software called 'SoilFluxPro'. The biggest advantage of the new observation technique is that time-series changes in greenhouse gas concentrations can be immediately confirmed in the field. It can also be applied to small areas with various treatment conditions, and it is simpler to use and requires less effort for installation and maintenance than the eddy covariance system. However, there are also disadvantages in that the observation system is still expensive, requires specialized knowledge to operate, and requires a lot of manpower to install multiple collars in various observation areas and travel around them to take measurements. It is expected that the new observation technique can make a significant contribution to understanding the CH4 emission pathways from rice paddies and quantifying the emissions from those pathways.