• Korean journal of applied entomology
• /
• v.45 no.2 s.143
• /
• pp.189-194
• /
• 2006

Fumigant toxicity of 18 plant essential oils were tested against the adults of oak longicorn beetle, Moechotypa diphysis. Among them, eucalyptus, rosemary and pennyroyal oils showed 100% mortality and sage oil showed 85% mortality at 10 $\mu\ell/\ell$ (air) dose. Eucalyptus and rosemary oils showed 100% mortality within 6 hr after treatment at 10 $\mu\ell/\ell$ (air) dose. GC and GC/MS analysis of the four essential oils and bioassay of their components revealed that 1,8-cineole (a major component of eucalyptus, rosemary and sage oils), thujone (a major component of sage oil) and pulegone (a major component of pennyroyal oil) showed higher adulticidal activity than others.

• Korean journal of applied entomology
• /
• v.46 no.2
• /
• pp.303-311
• /
• 2007

From the course of screening of useful xylanase producing microorganism from a phytophagous longicorn beetle, we isolated an extra-cellular xylanase producing strain, Paenibacillus sp. HY-8 from the intestine of Moechotypa diphysis adult. On the basis of morphological, biochemical and phylogenetic studies of the new isolate was identified as a Paenibacillus species. Production of xylanase in this strain was strongly induced by adding xylan to the growth medium and repressed by glucose or xylose. The highest xylanase production was attained in the M9 media containing 1% yeast extract and 0.5% birchwood xylan when cultured at $25^{\circ}C$ for 24 hrs. HY-8 producing xylanase showed superior hydrolytic activities against various plant source feedstuff than control xylanase produced by Tricoderma sp. at pH 6.0.

• Korean Journal of Environment and Ecology
• /
• v.7 no.2
• /
• pp.207-212
• /
• 1994

The census of Amphibia fauna in Tokyusan National Park was carried out during April 10, 1993 and August 8 - August 9, 1993 at the areas of construction field of golf course in Muju Resort and the Chibong. There were 6 species of 5 genera on the 4 families, 2 orders of Amphibia in Tokyusan National Park including Hynobius leechii. Onychodactylus fischeri, and Bufo bufo gargarizans which were announced the specific wild animals with the object of conserving by The Ministry of Environment. At the area of construction field of golf course in Muju Resort, there were 5 species of Amphibia observed as Hynobius leechii, Bombina orientalis, Bufo bufo gargarizans, Rana nigromaculata, and Rana dybowskii. Onychodactylus fischeri and Rana dyhowskii were observed at the Chibong area. The Chibong inwhich was observed 9 relatively large number of individuals of Onychodactylus fischeri was revealed as an environmentally sound habitat to Onychodactylus fischeri. Whereas in the construction field of golf course, there were intended to diminishing species and population size rapidly by progress with construction works. Therefore the large construction works such as golf couse and ski slopes which were concerning to deteriorate the natural environment should be restrain in National Park for animal diversity conservative.

• Korean Journal of Environment and Ecology
• /
• v.11 no.1
• /
• pp.84-99
• /
• 1997

Amphibian and Reptilian specimens which were surveyed in Gyebangsan during both from Apr. 26, 1995 to Oct. 15, 1995 and from Apr. 13, 1996 to July 28, 1996 were observed 9 Species in 5 Families on 2 Orders and 10 Species in 3 Families, 5 Fimilies, 9 Species and Reptiles were 2 Suborders, 3 Families on 2 Suborders, respectively. Bombina orientalis was revealed dominant species and Bufo stejnegeri was distributed abundant on this investigation area than other survey studies. Relatively large number of larvae of Rana dybowskii were found among the stream and small ponds at the Suchung-gol valley. There were 5 species of Specific wildlife species in Korea as Hynobius leechii, Rana dybowskii, Onchodactylus fischeri, Bufo stejnegeri and Bufo bufo gargarizans. Meanwhile Bufo stejnegeri were endemic species in Korea. 6 Species among 10 Species which were observed Reptiles in this survey Scincella laterale laterale, Dinodon rufozonatus rufozonatus, Agkistrodon brevicaudus, Agkistrodon saxatilis, Amphiesma vibakari ruthveni and Enhydris rufodorsata were Specific wildlife in Korea. Scincella laterale laterale and Takydromus amuriensis were habitated very abundant in Suchung-gol region. Habitat density of Takydromus amureinsis at Suchung-gol region was 36 individuals/ha. Total number of pressed to death individuals caused by press on the road from May to July, 1995 were 404 specimens in 10 Species. The 1,2,3 and 4 Stations where were found the largest number of death individuals caused by press on the road should considered to construct Eco-corridor to reduced the death individuals with small pipes beneath the road as a migration route. Onchodactylus fischeri have around 500m as moving distance and home range for lifelong and it were the climax of broad-leaved tree and highly moisture area that abundantly region of Onchodactylus fischeri.

• Korean Journal of Environment and Ecology
• /
• v.34 no.3
• /
• pp.207-215
• /
• 2020

In this experiment, investigated toxicity evaluation of chemicals using Asian toad embryos, along FETAX(Frog Embryo Teratogenesis Assay-Xenopus) protocol. Asian toad, Bufo gargarizans embryo incubated and investigation of Benomyl(Germicide), Carbofuran(Insecticide) and Thiobencarb(Herbicide) effect by probit analysis. As a result, depends on the concentrations of Benomyl, Carbofuran and Thiobencarb, along mortality and malformation rates were increases and larval body length were decreased. The teratogenic concentration(EC₅₀) of Benomyl, Carbofuran and Thiobencarb were 1.03, 8.74, 4.98mg/ℓ, respectively. And when exposed to Benomyl, larvae responded most sensitively to malformations. Embryo lethal concentration (LC₅₀) Benomyl, Carbofuran and Thiobencarb were 7.26, 560.72, 16.87mg/ℓ, respectively. And Benomyl were at the lowest concentration of lethal the embryos. The teratogenic index(TI) were 7.05 in Benomyl, 64.16 in Carbofuran and 3.39 in Thiobencarb, thus TI values were above 1.5, which is the criterion of teratogenicity. Three of the pesticides used in this study were considered to be a teratogenic substances and Carbofuran was the most potent teratogen. And more specific researches are needed to investigate the effects of pesticides on the embryo development of toads and amphibians and their mechanism.

• Korean Journal of Environmental Biology
• /
• v.35 no.4
• /
• pp.706-714
• /
• 2017

An investigation of the effects of Pb for domestic anuran embryos were evaluated with the Frog Embryo Teratogenesis Assay; Xenopus (FETAX). Depending on the species, the difference between the embryo size and the embryonic development time was determined. As a result, mortality and malformation rates were increased, malformation patterns were changed and larval body length were decreased in a dose dependent manner of the Pb. The half maximal lethal concentration ($LC_{50}$) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.58, 0.49, 0.52, $0.54mg\;L^{-1}$, respectively. The half maximal effective concentration ($EC_{50}$) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.35, 0.74, 0.30, $0.29mg\;L^{-1}$, respectively. The teratogenic index (TI) were 1.66 in the Bufo gargarizans, 1.81 in the Hyla japonica, 1.73 in the Rana nigromaculata and 1.86 in the Bombina orientalis, respectively. Therefore, the Pb seems likely to have a teratogenic effect in all four species' embryonic development. The Bombina orientalis was the most sensitive to the Pb. This means that the difference between the different species, even if they have all been exposed to the same concentration of pollutants depending on the species. The result above show that the Pb acts as a teratogenic agent in the development of the four domestic frog species.

• Proceedings of the Korean Society of Applied Entomolohy Conference
• /
• 2002.11a
• /
• pp.166-166
• /
• 2002

• Korean Journal of Veterinary Research
• /
• v.37 no.1
• /
• pp.9-13
• /
• 1997

The gastrointestinal endocrine cells of the African clawed toad, Xenopus laevis have been investigated immunohistochemically using the avidin-biotin method. Seven antisera were tested and three endocrine cell types immunoreacted with antisera to neurotensin, GRP and substance P. A moderate number of neurotensin-immunoreactive cells were weakly reacted in the small intestine. GRP-immunoreactive cells were mainly situated among the upper portion in the fundic glands, and the basal portion in the pyloric glands. These cells were oval and round in shape. On the other hand, in the intestine they were thin spindly cells with the epithelium. Substance P-immunoreactive cells were observed in among intestinal epithelium. However, no secretin-, motilin-, M-Enk- and PYY-immunoreactive cells were found in the GIT of the African clawed toads.

• The Korean Journal of Zoology
• /
• v.16 no.3
• /
• pp.193-201
• /
• 1973

A cellulose acetate electrophoretic survey of Korean Anura has revealed the presence of diverse lactate and malate dehydrogenase isozymes. The LDH and MDH isozymes in the tissues of the brain, heart, stomach, skeletal muscle and liver of the six species of Anura examined show the species specific patterns which differ from those of mammals and birds. Two isozymic forms of LDH and MDH exist in both Rana nigromaculata and Rana nigromaculata coreana, respectively, with almost the same pattern. LDH of Bombina orientalis has five isozymic forms, and that of Rana temporaria ornativentris contains four isozymes. Bufo sp. has 3 to 5, and Rana rugosa has 3 to 4 isozymic bands according to the tissues. MDH's of all animals have two isozymic forms with different spacing on the zymograms.

• The Korean Journal of Pesticide Science
• /
• v.12 no.3
• /
• pp.302-306
• /
• 2008

This study was performed to investigate the contact toxicity, fumigant toxicity, and synergistic effect using agricultural materials (AM) when mixed with selected essential oils against oak longicorn beetle, Moechotypa diphysis. Four kinds of AM, namely, chungkiller, ungsalta, jinap, purunjunsa showed 100% contact toxicity at one day after treatment. Subsequently, four AM was tested the fumigant toxicity during 24 hours. At concentration of 1 mL, jinap and chungkiller showed 100% mortality at 3 hr after treatment, prunjunsa and ungsalta showed 100% mortality at 6 hr after treatment. When applicate to the small vinyl bag (15 L), all AM (50 mL/15 L) was showed 100% mortality. Jinap showed the mortality 100% when mixed pennyroyal oil with the ratio of 5+1. It was higher than single treatment.