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Exploration on the Strategies of Organizing Curriculum for Improvement of Major Basic Competencies in the Agricultural High School Students to University by Departments Identical to Their Major (농업계 고등학생들의 동일계 대학 전공기초능력 향상을 위한 교육과정 편성 방안 탐색)

  • Kim, Jin-Gu;Lee, Gun-Nam
    • Journal of vocational education research
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    • v.29 no.3
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    • pp.61-83
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    • 2010
  • The purpose of this study was to analyze high schools' general and special subject required to successfully complete same stream curriculum which is identical to their major from agricultural high school, and to offer basic data on strategies of organizing agricultural high schools' curriculum for improving universities' major basic competencies. Using purposeful sampling technique, the professors of 116 universities professors in 8 agricultural university were analyzed through the survey research. The result was as follows. first, it appeared that for successful completion of major subjects of the same stream university, the basic science subject such as biology and chemistry has high relation with major basic ability, however math and physics are related highly in agricultural machine and agricultural civil engineering department, economics and math are in agricultural produce distribution department. Second, the basic ability such as linguistic competence and foreign language ability are essential to complete major subject. Third, if we look into relation of agriculture and life science industry stream specialized subject with major basic competencies, we can find considerable similarity between major field of university and subject name of specialized high school. Fourth, the main opinion is that basic concept and principle, laws of nature are should be main contents which is able to be practical, however experiment and practice is in food processing department, and academic theory is in biotechnology department.

Gender politics and the monster-abject representation method of the posthuman age. - Focused on works by Kim Eon-hee and Han-Kang - (포스트휴먼 시대의 젠더정치와 괴물-비체의 재현방식 - 김언희와 한강의 작품을 중심으로 -)

  • Baik, Ji-yeon
    • Cross-Cultural Studies
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    • v.50
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    • pp.77-101
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    • 2018
  • Even in our modern era, the projection of monsters in the recent literature contains the critical imagination of human existence for the posthuman age. The meaning of the monster-abject, especially as from the perspective of feministic criticism, contains criticism of the violent and oppressive patriarch as observed in the modern times. This article focuses on the gendered imagination of the discussions of the "abject" discussed by Julia Kristeva, and the "monstrous femine" discussed by Barbara Creed. Kim Eon-Hee's poems and Han Kang's novels, which have been examined extensively for analysis, show that the practical strategy of abject that goes beyond hate and sublime, wonder and joy through the imagination and concepts of monsters. The monster-abject strategy of Kim Eon-Hee's poem can be summarized by the narrative method of mirroring and the imagination of the truncated body. Mirroring falsification, which mimics the male speaker, is a method that some feminists strategically utilize in relation to the problem of female aversion in recent years as noted in the literature. In Kim Eon-Hee's poem, "becoming a man" and "imitating a man," through the method of mirroring appear as an image of cutting to dismantle the body. In that way, the narrative strategy of the abject that draws out abominations and bizarre effects which contains a strong critique of the patriarchal dominant ideology. The monster-abject strategy of Han-Kang's novel is embodied through the being of plants and the process of vegetarian-anorexia process. The world of the adject which was oppressed in the Han-Kang's novel, returns to the senses of the body through the symbol of the body. It is noted that the fictional characters who realize the repressed desire through the pathological symptom expressed by the female, go on to body perform active transformation. The sense of a body in a novel is not only a rejection of the world of animalman-civilization, but also a radically questioning of the noted and recognized boundaries between human beings and non-human being entities. The two writer's works show that the imagination of the monster-adject is not limited to rejecting the existing gender categories, but also goes in the direction of exploring the possibilities of various associated gender actions.

Modulation of Stress Protein Gene Expression by Environmental Stress and pH in the Mouse Fibroblasts and SCK Tumor Cells (생쥐의 纖維芽細胞와 SCK 腫瘍細胞에서 Stress와 pH에 의한 Stress Protein 遺傳子發見의 調節)

  • Kang, Man-Sik;Lee, Chung-Choo;Lee, Bonggeun;Suh, Mi-Young
    • The Korean Journal of Zoology
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    • v.28 no.2
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    • pp.108-119
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    • 1985
  • Aimed at elucidating the modulation of stress protein gene expression, the effect of environmental stress and pH on the induction of stress protein synthesis has been analyzed using SDS-polyacrylamide gel electrophoresis. Although the general patterns of protein synthesis in MEF and SCK cells are different, stress protein patterns are identical in both cells. Among three stress proteins, the $SP_70$ exhibits an interesting kinetics of induction and decay. The kinetics of $SP_70$ under acidic or normal pH appears to be similar, but the degree of hyperthermia and duration of treatment required for maximum induction are found to be different, being lower temperatures and shorter durations under acidic pH compared to those under normal pH. Inducation of stress protein and the accumulation of mRNA coding for stress proteins are blocked with actinomycin D, indicating the new RNA transcription is required for stress blocked with actinomycin D, indicating that new RNA transcription is required for stress protein induction. Treatment of cycloheximide during the after hyperthermia indicates that no specific protein is required for the induction of stress protein synthesis. Based on our preliminary data, we postulate that induction of stress protein synthesis in MEF and SCK cells is regulated primarily at the level of transcription and that $SP_70$ autoregulates its synthesis and levels of this protein are correlated with the stresseed state of a cell.

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Current biotechnology for the increase of vegetable oil yield in transgenic plants (식물 지방산 생산량의 증진을 위한 생명공학 연구현황)

  • Lee, Kyeong-Ryeol;Choi, Yun-Jung;Kim, Sun-Hee;Roh, Kyung-Hee;Kim, Jong-Bum;Kim, Hyun-Uk
    • Journal of Plant Biotechnology
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    • v.38 no.4
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    • pp.241-250
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    • 2011
  • The most part of vegetable oils is accumulated as storage lipid, triacylglycerol (TAG) in seed and used as energy source when seed is germinated. It is also used as essential fatty acids and energy source for human and animal. Recently, vegetable oils have been more and more an important resource because of the increasing demand of vegetable oils for cooking and industrial uses for bio-diesel and industrial feedstock. In order to increase vegetable oils using biotechnology, over-expressing or repressing the regulatory genes involved in the flow of carbon into lipid biosynthesis is critical during seed development. In this review, we described candidate genes may influence oil amount and investigate their potential for oil increase. Genes involved in the regulation from biosynthesis of fatty acids to the accumulation oils in seed can be classified as follows: First, genes play a role for synthesis precursor molecules for TAG. Second, genes participate in fatty acid biosynthesis and TAG assembly. Lastly, genes encodes transcription factors involved in seed maturation and accumulation of seed oil. Because factors/genes determining oil quantity in seed is complex as mentioned, recently regulation of transcription factors is being considered more favorable approach than manipulate multiple genes for increasing oil in transgenic plants. However, it should be figured out the problem that bad agricultural traits induced by the overexpression of transcription factor gene.

Early Life History of the Marine Animals 1. Egg Development, Larvae and Juveniles of Chaenogobius laevis (Steindachner) (해산동물의 초기생활사에 관한 연구 1. 미끈날망둑, Chaenogobius laevis (Steindachner)의 난발생과 자치어)

  • KIM Yong Uk;HAM Kyeong Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.22 no.5
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    • pp.317-331
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    • 1989
  • Chaenogobius laevis inhabit brackish waters. Adult male protects egg mass laid under the stones. The egg with small oil globule varied from 3.40 to 4.04 mm in diameter. When water temperature is $22.0\~23.0^{\circ}C$, larvae hatched about 113 hours after fertilization. The newly hatched larvae reared for 33 days grow up to 12.10 mm in total length and developed to the jevenile. When larvae length attained about 8.0 mm, jaw bones were more rapidly ossified than vertebrae and cranium.

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A Study on Korean Shamanistic Myth 'Woencheongang-Bonpuri' from a Perspective of Analytical Psychology (원천강(袁天綱)본풀이(本解)의 분석심리학적 관점에서의 고찰)

  • Kwang Ja Lee
    • Sim-seong Yeon-gu
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    • v.29 no.1
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    • pp.46-81
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    • 2014
  • The 'Woencheongang-Bonpuri' is one of the shamanistic myths in Cheju Island. That is a history of ancestor God of the 'Woencheongang'. This myth contains a shaman's initiation. And we can find important archetypal images in it. There are a child, old wise woman, book, water, tree, snake, Yakwangju which is a gem that emits light in the dark, dragon, hierogamy, goddess, time, etc. I've tried to interpret these images through the method of amplification from the perspective of analytical psychology. A heroine of this shamanistic myth is 'Ohnuri' that means 'today'. When she came out from the earth, she was a 'abandoned child' which is a 'hero-motif' in the fairytale and also a theme of individuation process in the analytical psychology. In the mean time, she grew up with helps of the nature, crane, and 'Yakwangju'. One day, she was found in the field by the people. They gave her a name of 'Ohnuri' and one day, an old wise woman told her how to go to her parents. On her way to the 'Woencheongang' where her parents regulate four seasons, she had met many characters which were in stuck. They told willingly Ohnuri a way to go to the 'Woencheongang'. Instead, they wanted to get solutions of their difficult problems. Finally, she met her parents in the 'Woencheongang' with joy and they taught her how to solve problems. Therefore, all characters in this myth could go on their own individuation process fortunately with help of the Whoencheongang's wisdom. In the meanwhile, Ohnuri got Lotus and Yakwangju and then she transformed to the goddess of the highest of the heavenly gods. Then she had helped people who get in trouble. In this way, the 'Woencheon-Bonpuri' was psychologically interpreted from aspect of analytical psychology.

Food habits, health behaviors related to the personality in Korean college students (대학생의 성격요인과 식습관 및 건강관련행태)

  • Kim, Nahyeon;Kim, Jinhee;Kye, Seunghee
    • Journal of Nutrition and Health
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    • v.53 no.1
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    • pp.13-26
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    • 2020
  • Purpose: This study identified the relationship between dietary habits and health-related behaviors depending on the Big Five personality factors (extraversion, openness, agreeableness, conscientiousness, neuroticism). Methods: The NEO-II test was administered to 337 male and female college students in Seongnam City, Gyeonggi Province, and their dietary habits and health-related behaviors were surveyed. Results: The male participants showed higher scores for extraversion, openness, agreeableness, and conscientiousness compared to that of their female counterparts, while the female participants showed higher scores for neuroticism. As for the results of multivariate logistic regression analysis, in the case of men, higher scores for extraversion were related to a lower intake of instant/fast foods and a higher intake of vegetables; higher agreeableness scores were related to a lower intake of fruit; and higher neuroticism scores were related to a heavy intake of high-cholesterol foods. It was found that higher openness scores were associated with a higher intake of burnt fish/meat and a lower intake of animal fat, while higher agreeableness scores were related to a lower intake of burnt fish/meat in women. Also, those subjects with higher openness and agreeableness scores were found to better consider the nutritional balance when having a meal. In the case of the male participants, higher openness scores were related to increased physical activity, while higher neuroticism scores were related to increased smoking and a lack of sleep. As for the women, those with higher extraversion scores smoked more, while those who recorded higher agreeableness scores were involved in more physical activities. Conclusion: Differences were observed in dietary habits and health-related behaviors between men and women depending on personality factors, and the analysis results of some dietary habits according to personality factors were inconsistent with those of the overseas studies. Therefore, to provide customized nutritional counseling when considering each individual's personality factors, more research results from domestic samples should be collected and accumulated.

The Growth Kinetics of S. aureus Inoculated onto Potentially High Risk Foods in School Foodservice Operations (학교급식에서의 잠정적 위험식품에 접종된 Staphylococcus aureus의 증식변화)

  • Choi, Jung-Hwa;Kim, Eun-Jung;Yoon, Ki-Sun;Kwak, Tong-Kyung
    • Korean journal of food and cookery science
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    • v.26 no.3
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    • pp.335-345
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    • 2010
  • The objective of this study was to model the kinetics of S. aureus survival on high risk foods in school foodservice operations. After inoculating S. aureus ATCC25923 onto the various high risk foods, the effects of competitive microorganism, storage temperatures($25^{\circ}C$, $35^{\circ}C$), and initial contamination levels ($1.0{\times}10^2\;CFU/g$, $1.0{\times}10^5\;CFU/g$) on the growth of S. aureus were investigated. Lag time decreased and specific growth rate increased with a storage temperature ($25^{\circ}C$<$35^{\circ}C$) and with a higher initial inoculation level ($1.0{\times}10^2\;CFU/g$<$1.0{\times}10^5\;CFU/g$). Previously it was shown that S. aureus is a weaker competitor than other organisms, but it proliferates aggressively in a noncompetitive environment. However, in our study, when S. aureus was used to inoculate japchae (glass noodles with sauteed vegetables) and meat ball, the growth of S. aureus was similar and more active with competitive organisms than that without competitive organisms. Regardless of other factors, the initial level of S. aureus was a more significant factor of the growth. High inoculation levels of S. aureus were reached at 6 log CFU/g within 3 hours. An incubation temperature of $35^{\circ}C$ and the animal protein component of menu items also were identified as significant factors influencing the growth of S. aureus. Therefore, the duration of time meals are stored before serving should be considered a critical control point. Food service providers must control time and temperature to insure the safety of cooked foods.

Endogenous Phenoloxidase Purified from an Earthworm, Lumbricus rubellus (붉은 지렁이(Lumbricus rubellus) 체내로부터 정제한 Phenoloxidase)

  • 백승렬;조은정;유경희;김유삼;서정진;장정순
    • The Korean Journal of Zoology
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    • v.39 no.1
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    • pp.36-46
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    • 1996
  • An endogenous phenoloxidase (EPO) from earthworm, Lumbricus rubellus, has been purified and characterized. The purified EPO using ammonium sulfate fractionation, Blue-2, Phenyl-, and Q-sepharose chromatography steps was revealed in SDS-PAGE as a single protein banri with Mr. of 59 kl)a. A native strudure of the enzyme was examined with an in situ staining of a nondenatudng-PAGE using DL-dopa as a substrate. The result showed that a single band due to the EPO activity was located siighdy above a standard polypeptide with Mr. of 210 kl)a. These fads indicate that the EPO is an oligomeric enzyme. The presence of a monophenolase activity of the purified EPO, which hydroxylates tyrosine to dopa, was confirmed by observing dopachrome accumulation at 475 nm at PH 8.0 with a typical lag phase during 60 mm. of meausrement. A series of inhibition study has been performed for the enzyme with several divalent cation chelators such as phenyithiourea (Flu), 1, lO-phenanthroline, EDTA, and EGTA. Among them, only V'flj inhibited the enzyme with 1C0.5 of 65 MM, which indicated that copper was critical for the catalysis of EPO. The enzyme was maximally active at 35'C and pH 8.0 when L-dopa to dopachrome conversion was spectrophotometricaily monitored at 475 nm. The apparent Km values of P0 for L-opa were obtained as 1.86 mM and 13.8 mM at pH 6.5 and 8.0, respectively. The catalytic efficiencies at both pH were almost identical [(kat/Km)pH8.0/(kcat/Km)pH6.5 = O.92] while the Vmax at p11 8.0 was 6.6-fold higher than that at pH 6.5. This fact may indicate that pH affeds the catalysis at substrate and/or enzyme-substrate complex level rather than the enzyme itself. Taken together, the EPO was an oligomeric enzyme which did not require proteolysis for its activation. These results also indicated that the enzyme can exist, at least, in part as a latent form In vivo, which might be distinct from the prophenoloxidase activating system. Therefore, it is pertinent to consider that there must be certain regulatory molecules or phenomena in L. rubellus which make the 1,0 in a latent form in vivo before the foreign invasions.

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Effects of Lycopene on the Expression of Lipid Metabolism, Glucose Transport and Pro-Inflammatory Related Genes in Chickens (라이코펜 첨가 급여가 닭의 지방대사, 포도당 수송 및 친염증 유전자 발현에 미치는 영향)

  • Jang, In Surk;Moon, Yang Soo
    • Korean Journal of Poultry Science
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    • v.42 no.3
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    • pp.231-238
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    • 2015
  • The present study aimed to investigate the effects of lycopene on hepatic metabolic- and immune-related gene expression in laying hens. A total of 48 25-week-old White Leghorn hens were randomly allocated into four groups consisting of four replicates of three birds: control (basal diet), T1 (basal diet + 10 mg/kg of tomato powder-containing lycopene), T2 (basal diet + 10 mg/kg of micelles of tomato powder-containing lycopene), and T3 (basal diet + 10 mg/kg of purified lycopene). Chickens were fed ad libitum for 5 weeks, and then total RNA was extracted from the livers for quantitative RT-PCR analysis. Peroxisome proliferator-activated receptor ${\gamma}$ (PPAR${\gamma}$) expression was decreased in the liver of chickens after lycopene supplementation (P<0.05). Micellar lycopene supplementation decreased the expression of PPAR${\gamma}$ target genes including fatty acid binding protein 4 (FABP4) and fatty acids synthase (FASN) in the T2 group (P<0.05). Sterol regulatory element-binding protein 2 (SREBP2) and C/EBP-${\alpha}$ were also downregulated in hens fed with micellar lycopene (P<0.05). Glucose transporter 8 (GLUT-8) was upregulated in the T2 and T3 groups (P<0.05). However, the expression of carnitine palmitoyltransferase 1 (CPT-1) was not changed by lycopene supplementation. Pro-inflammatory cytokines such as tumor necrosis factor ${\alpha}$ (TNF-${\alpha}$) and interleukin 6 (IL-6) were downregulated by lycopene supplementation (P<0.05). These data suggest that the type of lycopene supplementation is critical and that micelles of tomato powder-containing lycopene may play an important role in the modulation of lipid metabolism and immunity in chickens.