• Title/Summary/Keyword: 동결 온도

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Development of the analytical method for pesticide residues in crops by using gas chromatograph / solvent free solid injector (GC / SFSI) (무용매 고체 시료 주입기(SFSI)를 이용한 작물 중 잔류농약의 기체크로마토그래피 분석법 개발)

  • Kim, M.R.;Lee, Y.;Park, B.J.;Choi, J.H.;Kim, I.S.;Shim, J.H.
    • The Korean Journal of Pesticide Science
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    • v.9 no.3
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    • pp.237-242
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    • 2005
  • This experiment was carried out to develop the fast and simple method for pesticide residue analysis by using solvent free solid injector (SFSI) and to validate the efficiency of the method developed for the residue analysis of the endocrine disruptor-like pesticides such as endosulfan, metribuzin, trifluralin and vinclozolin. The samples after freeze drying were sealed in glass capillary tubes and then introduced into the heated injector of gas chromatogaphy. The required pre-heating times were 1 min for endosulfan and trifluralin, 5 min for vinclozolin, and 10 min for metribuzin. The detection limits of endosulfan in chinese cabbages, metribuzin in lettuces, trifluralin in spinachs and vinclozolin in hot peppers were 0.05, 0.1, 0.05 and 0.05 ng, respectively and their recoveries were ranged from 74%, $98{\sim}107%$, $86{\sim}95%$ and $94{\sim}95%$, respectively. The detected level of metribuzine residue in field lettuce samples by using the SFSI was 0.6 ng/mg.

Development of Model for Structural Evaluation of Anti-Freezing Layer (동상방지층의 구조적 평가를 위한 모형 개발)

  • Lee, Moon-Sup;Heo, Tae-Young;Park, Hee-Mun;Kim, Boo-Il
    • International Journal of Highway Engineering
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    • v.14 no.3
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    • pp.25-32
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    • 2012
  • The thickness of anti-freezing layer has been empirically determined using the frost depth obtained from the freezing index and has not been generally considered as a structural layer in pavement design procedure. In fact, the anti-freezing layer makes a role in structural layer and enables to reduce the total thickness of pavement system. The objective of this study is to develop the statistical regression model for evaluating the structural capacity of anti-freezing layer using Falling Weight Deflectormeter(FWD) test data in asphalt pavements. The FWD testing was conducted at the embankment, cutting, and boundary area of various test sections to estimate the structural capacity of anti-freezing layer in different foundation condition. It is observed from this testing that the center deflections of pavement structure with anti-freezing layer are smaller than those without anti-freezing layer ranging from 0.4 to 82.6%. To determine the variables of statistical model, the correlation study has been conducted between various FWD deflection indexes and the anti-freezing layer thickness. It is found that the ${\Delta}BDI$(%)(${\Delta}Basin$ Damage Index(%)) is highly correlated with anti-freezing layer thickness. The ${\Delta}BDI$(%) model were developed for evaluating structural capacity of anti-freezing layer using linear mixed-effect models.

Production of Single-Cell Protein on Petroleum Hydrocarbon -V. Recovery and Purification of the Yeast Cell and Its Preliminary Animal Feeding Test- (석유탄화수소를 이용한 단세포단백질의 생산에 관한 연구 -V. 균체의 회수, 정제 및 예비 동물사육 시험-)

  • Pyun, Yoo-Ryang;Kwon, Tai-Wan;Chee, Kew-Mahn;Kim, Chun-Su
    • Korean Journal of Food Science and Technology
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    • v.4 no.4
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    • pp.252-258
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    • 1972
  • Methods of separating yeast cells from oil-water-cell emulsion and subsequent purification of the recovered yeast have been studied. In addition, the results of preliminary feeding experiments in which a yeast grown on gas oil was incorporated into chick rations are reported. According to the present study, it appears that the recovery of the yeasts would be easier at pH 9, since the emulsion is relatively more unstable. A class of surface active agent at a concentration of 0.3% was found to facilitate the separation of the yeast from the emulsion. The use of electrolytes such as NaCl and KCl were found to be most effective in breaking the emulsion. Solvent treatment using iso-propyl alcohol and its azeotropic mixture with hexane at $58^{\circ}C$ are particularly suitable for purification of the yeast. In the feeding experiment it was found that 5 percent of the fishmeal in the control ration could be replaced by the yeast with no adverse effect on performance. However, when 8 percent of the fish meal in the control ration was replaced by the yeast, some effect on live-weight gain of the chicks was observed.

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Enzymological Properties of the Alkaline AL-Protease from Arthrobacter luteus and Detection of Its Active Amino Acid Residue (Arthrobacter luteus로부터 유래한 염기성 AL-Protease의 효소학적 성질 및 활성 아미노산 잔기의 검색)

  • Oh, Hong-Rock;Aizono, Yasuo;Funatsu, Masaru
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.2
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    • pp.193-204
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    • 1984
  • The enzymatic properties of the alkaline AL-protease, which had been prepared from the crude zymolyase of Arthrobzoter luteus, was investigated together with its active amino acid residue. Complete inactivaton of the proteolytic activity of AL-protease by either DFP or PMSF was simultaneously accompanied by the loss of its lytic effect on the lysis of yeast cell wall. In the reaction, AL-protease showed the pattern of inactivation to decrease very slowly, as compared to that of chymotrypsin, and that enzyme and DFP were found to react with a molar ratio of 1 : 1. The preparation of AL-protease exhibited no hydrolytic activity in any substrates of polysaccharases, playing a significant role in the lysis of yeast cell wall. The optimum pH and temperature of AL-protease was pH 10.5 and $65^{\circ}C$, respectively. It also showed stability in the pH range from 5 to 11 and at the temperature below $65^{\circ}C$. Through the identification of the amino acid residue in the active site of the $^{32}P$-diisopropylph-osphorylated(DIP) AL-protease modified specifically with $^{32}P$-labeled DFP, AL-protease was found to be a DFP-sensitive which has a mole of active serine residue involved in its proteolytic activity per mole of the enzyme.

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Development of Frozen Vegetable Groundnut Product for Year-round Supply (주년공급을 위한 풋땅콩 냉동가공기술 개발)

  • 손영구;황종진;김선림;허한순;박장환;김석동;이춘기
    • Food Science and Preservation
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    • v.5 no.4
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    • pp.326-330
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    • 1998
  • Freeze storage technique is widely used for food processing to keep freshness and quality of the product. This technique was applied to fresh, unshelled groundnut to develop a new type of product which could maintain fresh taste and nutritive values even after several months of storage. The groundnut varieties, Daepungtangkong, Daekwangtangkong and Sindaekwangtangkong were grown at the experimental field of NCES in 1996. Immatured pods or groundnut were harvested around 20 to 3o days before full maturity, washed, and steamed at 100$^{\circ}C$ for 5 min. to stop enzyme activity. After vacuum packing (at -760mmHg for 10 min.) with 0.08mm polyvinyldichloride film, the pods were immediately frozen at -70$^{\circ}C$ for 24h and transfered at -20$^{\circ}C$ for long-term storage. Physico-chemical properties of frozen vegetable groundnut were investigated at 2 months after storage and compared to those of conventionally dried groundnut. After 2 months storage, the thawed kernels were very palatable with softness and fresh taste. Acid value and hardness (measured as the compression force on the probe of a texture analyzer) were much lower in frozen vegetable groundnut than those in the air-dried ones. Presence of free sugars is one of the important factors affecting groundnut taste, and the free sugar contents were considerably decreased in the frozen vegetable groundnut compared to freshly harvested groundnut. But in dried groundnut no free sugar was detected.

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Investigation of Functional Ingredients from Onion According to the Extraction Methods, Heat Treatment, and Storage Period (양파의 추출방법과 추출액의 살균·저장에 따른 기능성성분 분석)

  • Park, Hoon;Oyunzul, Ganbud;Suh, Sung-Who;Park, Young-Seo;Jang, Jae-Kweon;Chung, Myong-Soo;Choi, Young Jin;Shim, Kun-Sub
    • Food Engineering Progress
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    • v.13 no.2
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    • pp.92-98
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    • 2009
  • An investigation was carried out to study the effects of extraction, heat treatment, and storage temperature on functional ingredients of onion. Extracts of onion paste and freeze dried onion were prepared by treatments with several solvents including hot water, methanol, and ethanol, and then contents of functional ingredients such as total phenol, flavonoid, quercetin, and thiosulfinate were determined. The highest levels of functional ingredients were observed in samples extracted using water at 100$^{\circ}C$ or ethanol. The skin extract contained 60-fold more quercetin than onion paste extract, whereas no thiosulfinate was detected in onion skin extract. Heat treatment of onion extracts at 60, 80, 105, and 121$^{\circ}C$ did not effect on the levels of all functional ingredients tested. During four-week storage, the levels of total phenol, flavonoid, and quercetin were not significantly changed regardless of storage temperature. However, the total thiosulfinate was rapidly reduced as the storage temperature increased.

Extraction of starch from frozen potato whole-tissues using cellulase and its physicochemical properties (셀룰로오스분해효소에 의한 동결감자로부터 전분의 추출 및 물리화학적 특성)

  • Kim, Jaehyun;Kim, Hyun-Seok
    • Korean Journal of Food Science and Technology
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    • v.51 no.4
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    • pp.348-355
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    • 2019
  • This study investigated the impact of cellulase treatment on the extraction yield of potato starch (PS), and compared the physicochemical properties of PS by conventional (CSE) and enzymatic (ESE) starch extraction. In ESE, the PS extraction yield was predominantly influenced by reaction temperature, time and their interaction, compared to the cellulase concentration. When potatoes were treated for 8 h at $40^{\circ}C$ with 1.5% cellulase, the PS extraction yield was about 3.4-fold higher than that by CSE. Compared to CSE-PS, ESE-PS showed lower total starch contents and higher amylose contents, resulting in lower swelling factors and distorted pasting viscosity profiles accompanied by absence of peak and breakdown viscosities. However, ESE did not affect the gelatinization characteristics of PS. Overall results suggested that ESE can provide the highest yield of PS, and ESE-PS can be a potential starch source for extending the utilization of PS in food industries.

Vacuum Pressure Effect on Thermal Conductivity of KLS-1 (진공압에 따른 한국형 인공월면토(KLS-1)의 열전도도 평가)

  • Jin, Hyunwoo;Lee, Jangguen;Ryu, Byung Hyun;Shin, Hyu-Soung;Chung, Taeil
    • Journal of the Korean Geotechnical Society
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    • v.37 no.8
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    • pp.51-58
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    • 2021
  • South Korea, as the 10th country to join the Artemis program led by NASA, is actively supporting various researches related to the lunar exploration. In particular, the utilization of water as a resource in the Moon has been focused since it was discovered that ice exists at the lunar pole as a form of frozen soil. Information on the thermal conductivity of lunar regolith can be used to estimate the existence for ice water extraction by thermal mining. In this study, the vacuum pressure effect on thermal conductivity of KLS-1 was investigated with a DTVC (Dusty Thermal Vacuum Chamber). The reliability of KLS-1 was reconfirmed through comparison with thermal conductivity of known standard lunar regolith simulants such as JSC-1A. An empirical equation to assess thermal conductivity considering dry unit weight and vacuum pressure was proposed. The results from this study can be implemented to simulate lunar cryogenic environment using the DTVC.

Performance Evaluation of Fiber-Reinforced Concrete Compression Members Transversely Constrained by BFRP (BFRP로 횡구속된 섬유 보강 콘크리트 압축부재의 성능평가)

  • Lee, Gyeong-Bok;Lee, Sang-Moon;Jung, Woo-Young
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.42 no.5
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    • pp.607-616
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    • 2022
  • Corrosion and degradation of reinforced structures due to abnormal climates and natural disasters further accelerate the aging of structures. Coping with the decrease in structure performance, many old structures are being repaired and reinforced with low-weight and high-strength materials such as glass fiber composite material (GFRP). To further contribute, this paper focus on a more economical and eco-friendly material, basalt fiber composite (BFRP), which provide a more effective lateral constraint effect for seismic reinforcement. The main variables considered in this study are the curing temperature during the manufacturing of BFRP and the material characteristics of the target concrete member. The lateral constraint reinforcement effect was investigated through the evaluation of the performance of normal concrete and those with improved durability through fiber reinforcement. The reinforcement effect was 3.15 times for normal concrete and 3.72 times for fiber reinforced concrete, and the difference in reinforcement effect due to the improvement of the durability characteristics of the compression member was not significant. Lastly, the performance of the BFRP was compared with the results of the GFRP reinforcement from the previous study. The effect of the BFRP reinforcement was 1.18 times better than that of the GFRP reinforcement.

Assessment of the Correlation between Segregation Potential and Hydraulic Conductivity with Fines Fraction (세립분 함유량에 따른 동상민감성 지수와 수리전도도의 상관관계 평가)

  • Jin, Hyunwoo;Kim, Incheol;Eun, Jongwan;Ryu, Byung Hyun;Lee, Jangguen
    • Journal of the Korean Geotechnical Society
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    • v.37 no.12
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    • pp.47-56
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    • 2021
  • The cryosuction (negative pore pressure) in freezing soils causes groundwater migration from the frozen fringe to freezing front for ice lens formation. Frost heave and heaving pressure by ice lens cause damage to ground infrastructure. In order to prevent damage by the frost heave, various frost susceptibility criteria have been proposed. The SP (Segregation Potential) is the most widely used classification criterion for frost susceptibility in cold regions. The expansion of the ice lens by the migration of the groundwater is a key role in frost heave mechanism, and thus it is necessary to evaluate the hydraulic conductivity. In this paper, soil mixtures of coarse-fines (sand-silt) were prepared in various weight fractions and used for frost heave and column permeability test. For each case, the SP and the hydraulic conductivity were derived and correlations were analyzed. As a results, the transition threshold of the SP and the hydraulic conductivity were shown at 20% and 50% of the silt weight fraction, respectively. Although there are difference between these transition thresholds, these two coefficients show a specific correlation. In the future, additional study should be conducted for detailed analysis of the threshold transition values between SP and hydraulic conductivity.