• Horticultural Science & Technology
• /
• v.28 no.6
• /
• pp.990-995
• /
• 2010

This study was conducted to compare differences in fruit quality by harvest time of 'Fuji' apple fruit that was frozen on tree by unusual low temperature in that air temperature was under $-3.5^{\circ}C$ for 7 hours. Fruits were harvested at 1 day before, and 2 days and 6 days after freezing damage, respectively. Harvest's soluble solid contents in all treatments was over $14^{\circ}Bx$. Firmness and titratable acidity of fruit harvested after freezing damage was lower than those of fruit harvested before freezing damage. During cold storage, ethylene production of fruit harvested after freezing damage was higher than that of fruit harvested before freezing damage. The reduction in the level of fruit quality during cold storage of fruit harvested after freezing damage was more serious than that of fruit harvested before freezing damage. The reduction of fruit quality during subsequent ambient temperature for 1 week after cold storage of fruit harvested after freezing damage was higher than that of fruit harvested before freezing damage. In comparison of treatments that were harvested at different times after freezing damage, ethylene production and reduction in the level of fruit quality until 8 weeks of cold storage of fruit harvested at 6 days after freezing damage was lower than that of fruit harvested at 2 days after freezing damage. However, this difference by harvest time after freezing damage disappeared after 8 weeks of cold storage. Incidence of flesh browning was not affected by freezing at air temperature under $-3.5^{\circ}C$ for 7 hours.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.21 no.2
• /
• pp.213-218
• /
• 1992

The reaching time to the freezing point was to be fast in the order of 2% agar gel, 5% agar gel, 20% gelatin gel, pork, respectively. The freezing time and the passing time through the zone of the maximum ice crystal formation had linear relationship with the coolant temperature. The average diameter d$_{p}$ of ice crystal in a soybean protein gel and the moving of freezing front were represented an inverse proportion, and the moving velocity of freezing front was shown as 3.4$\times$10$^{-6}$ $\textrm{cm}^2$/sec from predicted theoretical formula. This value was very close to experimental results. The storage temperature did not give any influences for the growth of ice crystal in inside soybean protein gels during freezing conservation. The relationship between freezing condition and structure of freezing front was as follows : (moving velocity of freezing front) : (mass transfer rate of water at freezing point)$\times$(surface area of freezing front).

• Food Science and Preservation
• /
• v.6 no.4
• /
• pp.398-401
• /
• 1999

This study was conducted to investigate the changes in qualities of soft persimmon by freezing and defrosting. Testing varieties were Sangjudungsi and Chunsdobansi that were cultivated on Sangju and Chungdo regions, chief cultivation of astringent persimmon in Kyongbuk province. Dropping time to 40 degrees below zero of the flesh was 10∼20 minutes longer in Chungdobansi than that in Sangiudungsi. Freezing temperature of astringent persimmon was 2∼3 degrees below zero. Occurence rates of cracked fruit during freezing storage were 24.5% in Sangjudungsi and 15.5% in Chungdobansi. Defrosting of Sangjudungsi and Chungdobansi took 150 minutes and 120 minutes at 5$^{\circ}C$, respectively. L values of chromaticity were some lower after defrosting than that of frozen soft persimmon, and a and b values decreased during defrosting rapidly. Soluble solid contents of frozen soft persimmon almost didn't change during freezing, that is, harvesting, softening, frosting and defrosting steps. Defrosting completion time to core part took 4 hours and 30 minutes in Sangjudungsi and 4 hours and 20 minutes in Chungdobansi at ordinary temperature (10.9∼14.8$^{\circ}C$).

• Journal of the Korean Geotechnical Society
• /
• v.36 no.12
• /
• pp.17-25
• /
• 2020

The artificial ground freezing method is a ground amelioration technology that does not have a permanent effect on the ground. One of the key factors that determine the efficiency and design criteria of the artificial ground freezing is the groundwater flow. Therefore, in order to accurately evaluate the behavior of the artificial ground freezing, studies on the effect of water flow on the formation of ice walls must be preceded. In this paper, experimental and numerical analyses were conducted using only pure water to maximize the effect of water flow on the formation of ice walls. A hydro-thermal coupled model for freezing behavior was proposed and the accuracy of the model was verified. Through the numerical and experimental studies, the flow rate dominates not only the formation time but also the shape of the ice wall. In addition, this study proposes a method to indirectly predict the ice wall formation time, which is expected to be highly useful for a practical application where it is difficult to visually identify ice walls.

• 한국방재학회:학술대회논문집
• /
• 2011.02a
• /
• pp.83-83
• /
• 2011

도로의 동상방지층을 설치하기 위하여 동결관입깊이를 산정하며, 동결지수는 대기온도의 강도와 지속시간의 누가영향으로 표시된다. 대기온도는 일평균 대기온도를 사용하며 4개/일 평균(대기온도$_4$)을 사용하는 것이 일반적이지만, 현재 기상청에서 제공하고 있는 일평균 대기온도는 8개/일(대기온도$_8$)를 제공하고 있다. 이러한 일평균 대기온도의 산정하는 방법이 동결지수의 산정결과에 미치는 영향을 확인하기 위하여 한반도 남부지역(동결지수 $350^{\circ}C{\cdot}$일) 미만 6개지역을 저성토부, 절성경계부 및 절토부로 구분하여 15개소에 대하여 현장계측시스템을 구축하였다. 그리고 현장계측 시스템으로부터 얻어진 대기온도를 통하여 동결지수를 산정하여 비교하였다. 동결지수 비교결과 일반적으로 일평균 대기온도$_4$를 사용한 경우가 일평균 대기온도$_8$을 사용하는 경우보다 약 3% 전후 큰 것으로 나타나났으며, 6개 지역의 동결지수를 결정할 때 대기온도$_8$을 사용한 동결지수를 보정할 수 있는 보정식을 제안하였다.

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.10a
• /
• pp.132-132
• /
• 2003

멸종위험성이 높은 재래돼지를 유전자원으로서 안전하게 보존하고 유전적 다양성을 유지하기 위해 체내수정란의 동결보존 방법을 수행하였다. 재래돼지의 과배란유기는 altrenogest를 1일 20mg씩 18일 경구투여하고 PMSG 500~l,000IU 근육주사후 80시간에 hCG 500~750IU를 근육주사하였다. 발정이 관찰된 개체는 발정개시후 12시간과 24시간에 자연교배 또는 액상정액을 이용하여 2회씩 수정시켰다. 최종 수정후 5일째에 외과적으로 개복수술하여 FBS가 5% 첨가된 D-PBS 관류액으로 자궁으로부터 수정란을 회수하여 상실기, 배반포기, 확장배반포기의 수정란으로 구분하였다. 회수된 수정란은 FBS가 20% 첨가된 D-PBS의 0.4, 0.8, 1.4M glycerol 항동해제에 각 단계별로 10분씩 평형시킨후 수정란동결기(CL863, Australia)를 이용하여 18$^{\circ}C$부터 -7$^{\circ}C$까지 2$^{\circ}C$/min의 냉각속도와 -7$^{\circ}C$부터 -35$^{\circ}C$까지 0.5$^{\circ}C$/min의 동결속도(실험1), 1$^{\circ}C$/min의 냉각속도와 0.3$^{\circ}C$/min의 동결속도(실험 2)로 동결시켰다. 또한 FBS가 20% 첨가된 D-PBS의 ethylene glyco1(EG) 1.8M의 항동제에 15분간 평형시킨후 실험 1과 동일한 방법으로 동결시켰다(실험 3). 동결수정란의 융해는 37$^{\circ}C$의 항온수조에서 30초간 실시하였다. 항동해제로 glycerol을 이용한 수정란은 융해후 3가지 농도로 0.3M sucrose, 0.8M glycerol, 0.4M glycerol을 첨가한 D-PBS에 각각 10분씩 단계적으로 정치시킨 다음, 10% FBS 첨가 mNCSU-23으로 3회 세척했다. 항동해제로 EG를 이용한 수정란은 융해후 즉시 D-PBS에 각각 10분간 정치시킨 다음, 10% FBS 첨가 mNCSU-23으로 3회 세척했다. 항동해제가 제거된 수정란은 FBS가 10% 첨가된 mNCSU-23 배양액에서 39$^{\circ}C$, 5% $CO_2$ 배양기에 48시간 배양하면서 생존여부를 판단하였다. 실험 2에서 확장배반포배 수정란이 25.3%의 생존율을 나타내었으며, 실험 1과 실험 3에서는 수정란의 형태와 관계없이 생존성을 확인할 수 없었다. 이상의 결과로 보아 glycerol 완만동결에서는 확장배반포기 수정란 이상이 보존가능한 것으로 추정되나 더 추가적인 연구가 요구된다.

• Culinary science and hospitality research
• /
• v.14 no.1
• /
• pp.144-151
• /
• 2008

This study investigated the changes in the contents of allicin and diallyl disulfide and in the alliinase activity during the vacuum freeze drying of onion samples treated as the whole, sliced and crushed forms. The contents of allicin and diallyl disulfide in raw onions were 26.40ppm and 2.78ppm respectively. The contents of allicin and diallyl disulfide of onion samples treated as the whole, sliced and crushed forms increased with the progress of vacuum freeze drying. The degree of increase was different in each onion sample form prepared by vacuum freeze drying(p<0.05). The specific activity of alliinase in raw onions was 7.536 units/mg protein. The activity in onion samples treated as the whole, sliced and crushed forms decreased with the progress of vacuum freeze drying. The activity in the whole onion prepared by the vacuum freeze drying for 8 hrs reduced to 5.516 units/mg protein with 73.2% remaining and to 3.304 units/mg protein with 43.8% remaining for 36 hrs. The activity in the sliced onion prepared by the vacuum freeze drying for 36 hrs reduced to 2.366 units/mg protein with 31.4% remaining and the activity in the crushed onion prepared by the vacuum freeze drying for 36 hrs reduced to 2.232 units/mg protein with 29.6% remaining. The alliinase in onion sample treated as the whole form showed the highest remaining activity during the vacuum freeze drying.

• Journal of Soil and Groundwater Environment
• /
• v.11 no.5
• /
• pp.43-50
• /
• 2006

Bioreporter bacteria, such as recombinant bioluminescent bacteria, have been used for the detection of specific compounds in complex environmental media. In this study, optimum conditions for the preparation and application of deep-freezed and Iyophilized recombinant bioluminescent strain KG1206 were investigated for the future application on contaminated environmental sites. Genetically engineered microorganism, Pseudomonas putida mt-2 KG1206, contains TOL plasmid and the plasmid inserted $P_{m}$, promoter on the upper part of lux gone in vector pUCD615, and m-toluate and benzoate are considered direct inducers for bioluminescence. Optimum conditions determined for the preparation and application of the deep-freezed and lyophilized strain were followings: cryoprotective agent (24% sucrose), lyophilization time (12 hrs), strain concentration ($OD_{600}=0.6$), reconstitution for freezed strain (quick reconstitution at $35^{\circ}C$), reconstitution for lyophilized strain ($3{\sim}6$ hrs exposure on LB medium), carrying conditions (keep at $20^{\circ}C$ after reconstitution). These results demonstrate the feasibility of deep-freezed or lyophilized state of genetically engineered bioluminescent strain for environmental usage.

• Korean Journal of Animal Reproduction
• /
• v.23 no.4
• /
• pp.281-291
• /
• 1999

The objective of this study was to optimize the freezing/thawing method of in vitro produced Hanwoo blastocysts. Day 7 blastocysts after IVF were vitrified using EFS40 (40% ethylene glycol, 18% ficoll, 0.3 M sucrose and 10% FBS added m-DPBS) as a freezing solution and electron microscope (EM) grid (V-G) or straw (V-S) as an embryo container. In both method, freezing/thawing were treated by 2-step, treatment time was required in V-G method and V-S method, for 2 min / 3 min and 3.5 min / 10 min, respectively. Embryo survival was assessed as re-expanded and hatched rates at 24 h and 48 h after warming, respectively. The results obtained in these experiments were summarized as follows: when the effect of exposure in vitrification solution and chilling injury from freezing procedure on in vitro produced expanded blastocysts were examined, at 24 h after warming, embryo survival in exposure group (100.0%) was not different compared to that in control group (100.0%), although those results were significantly different with two vitrified groups (V-G: 87.8, V-S: 77.8%) (P＜0.001). However, at 48 h after warming, hatched rates of V-G group (67.8%) were significantly higher than those of V-S group (53.3%) (P＜0.05). In addition, this hatched rate in V-G group was not different with that in exposure group (73.3%). When the effects of embryo developmental stage (early, expanded and early hatching blastocysts) and embryo container (EM grid and straw) to the in vitro survival of vitrified-warmed day 7 Hanwoo blastocysts were simultaneously examined, fast developed embryos were indicated the better resistance to freezing than delayed developed one, irrespective of embryo containers (early; 57.1 ＆ 24.4%, expanded; 84.7 ＆ 60.6%, early hatching; 91.7 ＆ 80.0%) (P＜0.001). Especially, in expanded and early hatching blastocysts, embryo survival of V-G group (67.8, 95.0%) was significantly higher than those of V-S group (53.0, 65.0%) at 48 h post warming, respectively (P＜0.05, P＜0.001). Therefore, this study indicates that Hanwoo blastocysts can be cryopreserved more simple, efficient and successful by vitrification method using EM grid.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.19 no.6
• /
• pp.1059-1075
• /
• 2017

The objective of this study was to evaluate the freezing and thawing resistance of the existing drainage system for leakage treatment of underground concrete structures operating in cold regions. The freezing and thawing test was conducted on 4 types of drainage system specimens to evaluate the freezing and thawing resistance of the drainage system. The freezing and thawing resistance was evaluated on 4 types of Hotty-gel, as a waterproofing material, connection methods and on two methods to fix the drainage board with Hotty-gel on the surface of cement concrete specimen. One cycle of the freeze-thaw testing was 48 hours (24 hours of freezing and 24 hours of thawing), and the temperatures of freezing and thawing were at $-18^{\circ}C$ and $10^{\circ}C$, respectively. Among the 4 types of Hotty-gel connection methods, leakage occurred after 28 cycles (8 weeks) of freeze-thawing only in the Hotty-gel connection method with the 'V' groove applied to the corner of the drainage board. No leakage occurred in the 3 types of Hotty-gel connection methods. In two fixing methods, leakage occurred in the method of fixing the drainage board on the cement concrete specimen using the washer, screw and plastic wall plug. Leakage occurred at one point after 10 cycles (3 weeks) of freezing and thawing. After 28 cycles (8 weeks) of freezing and thawing, leakage point increased to 5 points. As time passed, the leak point was not increased, but the amount of leakage was increased at each leak point. The Hotty-gel connection method with cross-sectional diagonal shape was evaluated to be the highest in the production efficiency considering the production time and manufacturing method of the Hotty-gel connection shape. In the construction efficiency considering the construction time and construction method, the fixing method of air nailer, fixed nail and washer was superior to that of the washer, screw and plastic wall plug.