• Clinical and Experimental Reproductive Medicine
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• v.36 no.4
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• pp.311-319
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• 2009

Objective: Human embryonic stem cells (hESCs) have the capacity to differentiate into all of the cell types and therefore hold promise for cell therapeutic applications. In order to utilize this important potential of hESCs, enhancement of currently used technologies for handling and manipulating the cells is required. The cryopreservation of hESC colonies was successfully performed using the vitrification and slow freezing-rapid thawing method. However, most of the hESC colonies were showed extremely spontaneous differentiation after freezing and thawing. In this study, we were performed to rapidly collect of early passage hESCs, which was thawed and had high rate of spontaneously differentiation of SNUhES11 cell line. Methods: Four days after plating, partially spontaneously differentiated parts of hESC colony were cut off using finely drawn-out dissecting pipette, which is mechanical separation method. Results: After separating of spontaneously differentiated cells, we observed that removed parts were recovered by undifferentiated cells. Furthermore, mechanical separation method was more efficient for hESCs expansion after thawing when we repeated this method. The recovery rate after removing differentiated parts of hESC colonies were 55.0%, 74.5%, and 71.1% when we have applied this method to three passages. Conclusion: Mechanical separation method is highly effective for rapidly collecting and large volumes of undifferentiated cells after thawing of cryopreserved early passage hESCs.

• Food Science and Preservation
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• v.10 no.4
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• pp.459-465
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• 2003

This study was carried out to investigate the changes in internal pressure according to various freezing methods, as basic research to protect the destruction of tissues when fruits and vegetables are frozen. The rate of weight loss, caused by the freezing of fruits and vegetables, was found to be the least (0.44∼1.38％) when the immersion freezing method was applied. The difference in the rate of weight loss was the highest when freezing methods were applied to watermelon, and the freezing rate of watermelon whose moisture contents were greater have relatively greater influence on the weight loss. The difference in internal pressures was the least and caused by the volume increase and decrease, when pear, apple, and melon were frozen using the immersion freezing method, while the diffeyence the greatest when the air-blast freezing method was used. As the freezing rate was greater, the internal pressure was less. However, the internal pressure of strawberry and watermelon was the greatest when the immersion freezing method was applied. Frozen without using the thermal equalizing method, the change in internal pressure of fruits was about 2 psig. In contrast, the internal pressure of watermelon applied with the thermal equalizing method was changed in a way similar to that of watermelon not applied with the method, but the former generated a certain level of internal pressure and maintained a significantly low level of internal pressure (about 1.3 psig). When thawed, the internal pressure of samples to which the thermal equalizing method was applied was less than that of what the thermal equalizing method was not applied to. In comparison with the application of multi-step thermal equalizing method, 3∼4 times of application of the thermal equalizing method to the freezing resulted in the decrease of fluctuation range of internal pressure.

• Clinical and Experimental Reproductive Medicine
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• v.35 no.3
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• pp.203-212
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• 2008

Objective: During cryopreservation process, cold shock and cryo-injury affect the fertilizing capacity of the sperm by damaging cell membranes with loss of functional integrity. A longstanding concept for preventing the cryo-damage is to stabilize the plasma membrane by incorporating cholesterol. This study was to determine the effects of cholesterol in freezing media on the motility and functional integrity of human sperm after cryopreservation. Methods: Control group (non-cholesterol treated) and different concentrations of cholesterol-treated sperm (14 healthy males) were frozen and thawed. After freezing and thawing of sperm, the quality of sperm was evaluated by sperm analysis, acrosome reaction test and sperm chromatin structure assay. Results: When human sperm were incubated in sperm freezing medium (SFM) containing $0.5{\mu}g$ cholesterol and then freezing/thawing, the motility of sperm have significantly improved compared to those untreated cholesterol ($33.46{\pm}1.48%$ vs. $30.10{\pm}1.07%$, p<0.05). The rate of calcium ionophore-induced acrosome reactions in post-thawed sperm was significantly higher than that ($53.60{\pm}1.60%$ vs. $47.40{\pm}1.86%$, p<0.05) in SFM containing cholesterol. Sperm chromatin structure assay revealed that DNA damage to the sperm in the cholesterol-treated group was lower than that of non-treated group. Conclusion: These results suggest that increased cholesterol content of sperm plasma membrane by supplementation of cholesterol in SFM improves sperm motility, capacitation status, and DNA integrity. Therefore, addition of cholesterol into SFM could be a useful for protecting human sperm from cold shock and cryo-injury during cryopreservation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.12 no.3
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• pp.191-200
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• 1979

For the use of antarctic krill as a fond protein source its compositional characteristics were investigated as the first part of the work includes other subjects such as processing of drill paste, concentrates, and fermented or seasoned product. In general composition of fresh frozen and preboiled frozen krill on board, the contents of crude fat and free amino nitrogen were higher in the former than in the latter which contained a high amount of ash. VBN was rather high as much as 37.6 and $26.4\;mg\%$ in both fresh frozen and preboiled krill. The pH of drill homogenates was 7.1 to 7.2 in both cases. Such a low pH might be attributed to a long term storage and temperature fluctuations during frequent transshipping. The amino acid competition of fresh frozen krill meat showed relatively high amount of glutamic acid, aspartic acid, lysine, proline, and leucine while methionine, histidine, serine, tyrosine, and phenylalanine were lower. Among the essential amino acids lysine and leucine were higher and methionine was lower. In tile composition of free amino acid proline, lysing, arginine, and alanine were higher comparatively to the contents of histidine, aspartic acid, serine, and threonine. It is noteworthy for nutritional qualification that tile essential amino acids particularly as lysine were abundant similarly to that of fishes. Heavy metal contents of krill meat 0.039 to 0.048 ppm as Hg, 0.06 to 0.11 ppm as Pb, less than 0.32 ppm as Zn, 0.008 to 0.012 ppm as Cd, 0.61 to 0.68 ppm as Fe, 0.87 to 1.37 ppm as Cu, and nondetective as Cr. A high Cu content seems to be resulted by tile blood pigment of crustacea. The ratio,1 of edible portion to non-edible portion were 37:63 in fresh frozen and 42:58 in preboiled frozen krill respectively. Release of drip after thawing was more in fresh frozen than in preboiled frozen drill marking $36\%$ and $24\%$ of both respectively.

• Transactions of the Korean Society of Automotive Engineers
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• v.22 no.5
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• pp.13-19
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• 2014

Urea-SCR system is the selective catalytic reduction to reduce nitrogen oxides ($NO_x$) emitted from diesel vehicles. The objective of this study is numerical analysis of 3-dimensional unsteady melting problems of frozen urea by using an electric heater. It can be applied to determine capacity of power with respect to time and the location of the urea suction pipe in urea storage tank. The study includes the change of liquid volume fraction, temperature profiles and a influence of natural convection by using the commercial software STAR-CCM+(v7.06). The accuracy of the numerical analysis is estimated by comparisons with experimental data. After validation, a numerical analysis for freezing urea is conducted with four different heating power. From the results, it was found that relation of velocity of phase interface and amount of melting urea by increasing heating power in a container. There is also a difference in trend between velocity of phase interface and amounts of melting urea because of effect of natural convection.

• The Korean Journal of Food And Nutrition
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• v.10 no.4
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• pp.553-558
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• 1997

Frozen cod fillet block(7.0$\times$4.6$\times$0.6cm, 18$\pm$0.5g) was purchased from a supermarket in Taegu area. Three experimental groups of fish fillet, frozen-thawed cod fillet refrigerated at 5$^{\circ}C$(R), frozen at -2$0^{\circ}C$(F) and repeated freezing and refrigerating(RFR) every other day, were incubated at each temperature. Changes in the viable counts of mesophiles and psychrotrops, the amount of free drip and pH of cod muscle during cold storage were 6.5$\times$104 and 7.4$\times$103 CFU/g of muscle, respectively. The viable counts of cod muscle R and FRF exceeded 107 CFU/g within 8 days and 16 days, respectively, while the viable counts of cod muscle F were not exceeded 107 cells/g throughout the storage period. The viable counts of psychrotrops exceeded that of mesophiles at the end of cold storage period. The viable counts of cod muscle showed positive correlation with pH(r=0.73-0.96,the highest in RFR) during cold storage. The amount of free drip in cod muscle R, F and RFR was 27.06$\pm$9.75, 27.56$\pm$8.02 and 33.97$\pm$10.70%, respectively. The amount of free drip in RFR increased as the progress of storage.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.400-408
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• 2018

Biodiversity has continued to degrade in the $21^{st}$ century due to global warming occasioned by destruction of the environment around the world.. The Nagoya protocol places Korea in a unique position to effectively develop and protect its domestic genetic resources. Microalgae under study in this research contains large amount of antioxidant substances such as beta carotene and astaxanthin, that can be used as biological resource owing to the large amounts of biomass that can be secured through photosynthesis. However, it is difficult to preserve it since cryopreservation method used for long-term preservation is yet to be developed. A basic study for long term cryopreservation was carried out on Nizschia frustulum and Nitzschia amabilis which belong to marine diatoms. As cryoprotectants (CPAs), glycerol, DMSO, and methanol which penetrate into cells were prepared at 5%, 10%, and 15% concentrations each, in case of methanol, it was tested at concentrations of 5%, 10% and 12% by its nature. Two kinds of microalgae, N. frustulum and N. amabilis, were diluted with $10^2$, $10^3$ and $10^4cells\;ml^{-1}$, respectively. The highest survival rate was shown at12% concentration of methanol, and the figures were $6.94{\pm}0.31%$ in N. frustulum and $8.85{\pm}0.16%$ in N. amabilis. As a result of 3 weeks cultivation of thawed microalgae after freezing, the result is shows that N. frustulum increased about 10 times faster and N. amabilis increased about 12 times the original concentration.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.05a
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• pp.261-265
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• 2005

본 연구는 도살체중이 돈육의 등심, 목살, 삼겹살 및 햄 부위에 미치는 영향을 검토하였다. 동결감량과 해동감량은 도살체중에 의한 차이는 없었으나, 등심 및 햄 부위가 목살 및 삼겹살보다 높았다. 팬에 의한 가열감량은 도살체중에 의한 차이는 없었지만 등심, 목상 및 햄 부위가 삼겹살보다 높았다. 열탕 가열감량은 도살체중의 영향은 없었으나 등심이 가장 높고, 목살이 가장 낮았다. 돈육의 $L^{\ast}$, $a^{\ast}$ 및 $b^{\ast}$값은 도살체중의 영향이 없었다. Hardness와 springiness는 도살체중의 영향을 받지 않았다. SW110인 등심과 삼겹살의 cohesiveness가 SW90보다 높았으며, gumminess, chewiness 및 전단력가는 삼겹살이 가장 높았으며, 대체로 도살체중이 높은 것이 높았다. 가열육의 맛은 SW110 삼겹살이 가장 높았고, 향기는 SW110 목살과 삼겹살이 가장 높았다. 조직감, 다즙성 및 전체적인 기호성도 삼겹살과 목살이 우수하였다. 따라서 돈육의 기호성은 도살체중과 부위에 의한 차이가 있었다.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2006.05a
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• pp.77-82
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• 2006

본 연구는 제주 재래돼지에게 감귤 부산물을 급여하고 몇 가지 특성 및 기호성을 파악하고자 하였다. 감귤 부산물을 급여한 돈육 등심과 급여하지 않은 돈육 등심 사이에 pH, VBN 및 일반 세균수의 차이는 없었다. 그러나 TBARS 값은 감귤을 급여한 돈육등심이 유의하게 낮았다(p<0.05). 감귤 부산물을 급여하지 않은 TB-0구와 급여한 TB-1의 표면색도는 $L^*$ 및 $b^*$값은 유의한 차이가 없었으나, $a^*$값은 TB-0구가 TB-1구보다 유의하게 높은 경향이었다. 보수력 및 팬 가열감량은 TB-0가 TB-1보다 유의하게 높았으며(p<0.05), 동결감량, 해동감량, 열탕가열감량은 시료들 사이에 유의한 차이가 없었다. 경도, 탄성, 응집성, 저작성 및 전단력은 감귤 부산물 급여의 영향이 없었지만 뭉침성은 TB-1이 유의하게 높았다(p<0.05). 맛, 향기, 다즙성 및 종합적인 기호도는 감귤 부산물 급여에 따른 영향이 없었으나, 연도는 감귤 부산물을 급여한 돈육 등심이 유의하게 높았다(p<0.05).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.8 no.1
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• pp.37-45
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• 1975

The present study was ,conducted to evaluate the effects of condensed phosphates on the refeezing damage of Alaska pollack muscle. The fillets were dipped in such solution as 5 and $10\%$ sodium polyphosphate, 1 and $5\%$ mixture of sodium polyphosphate and sodium pyrophosphate (1:1, w/w) for 1 and 5 minutes, respectively, before refreezing. And fillets were frozen at $27^{\circ}C\~28^{\circ}C$ and stored for 15 days at $-18^{\circ}\~-20^{\circ}$. The degree of denaturation was estimated by determining amounts of drip relased, content of total solids, nitrogen, and DNA in the drip an cooking-weight-loss. Phosphorus absorbed in the muscle was also determined. Phosphorus absorbed in the fillets treated with loft solution of sodium polyphosphate for 5 minutes amounted to 101 mg/100g muscle as $P_2O_5$. The absorption was dependent on tile concentration of treating solution rather than on the dipping time. The increase of phosphorus absorption seemed to affect to reduction of drip. Among the treating conditions, $10\%$, 5 minutes and $10\%$ 1 minute with sodium polyphosphate appeared most effective ones on drip reduction. The effect of $5\%$, minutes with the mixture of sodium polyphosphate and sodium pyrophosphate did not show so benefitable effect in refrozen fillets. As a tendency total solids, nitrogen, and DNA in tile drip varied proportionally to the amount of drip released. And the content of DNA was lower than the amount. Treatment, at higher the concentration and longer the dipping time, resulted in the lower cooking-weight-loss and the better quality on organoleptic test of thawed fillets.