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The Study on the Ultrastructure and Distribution of Dopaminergic Cells in the Brain of Mongolian Gerbil after Water Deprivation (절수에 의한 Mongolain gerbil 뇌 Dopamine성 면역반응세포의 분포와 미세구조의 변화에 관한 연구)

  • Song, Chi-Won;Lee, Kyoung-Youl;Park, Il-Kwon;Kwon, Hyo-Jung;Kim, Moo-Kang;Lee, Kang-Lee
    • Applied Microscopy
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    • v.30 no.2
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    • pp.193-204
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    • 2000
  • Nowadays, mongolian gerbil is widely utilized in the research of brain and water deprivation because of congenitally incomplete Willis' circle, audiogenic seizure in low noise, and special cholesterol metabolism without water absorption for a long time. In this study, we intended to identify the time lapse changes in the general morphoogy and ultrastructure of the catecholaminergic neurons of mongolian gerbil brain in after long-term water deprivation. Fifteen mongolian gerbils were divided into 3 groups (5, 10, and 20-day water deprivation groups), each with 5 mongolian gerbils. Additional 5 mongolian gerbils which received water without limitation were used as a control. The brain sections were immunostained using tyrosine hysroxylase (TH), $ dopamine-\beta-hydroxylase$ (DBH), and phenylethanolamine-N-methyltrasferase (PMNT) antibodies. And immunoreactive cells were observed by electromicroscopy for the ultrastructural changes . The TH-immunoreactive (TH-IR) nerve cells were observed in the para- and peri-ventricular nucleus of the 3 rd ventricle in the hypothalamus and the substantia nigra. The number of TH-IR neurons in these areas was decreased from the 5th day of the water deprivation to the 10 th day and reincreased until 20 th day water deprivation. The shape and density of the dopamine-secreting cells identified by immunohistochemistry showed changes in the continuous water deprivation. Electron microscopy revealed a round nucleus in the neurons of control group but 5-day water deprivation group showed a dense and irregularly shaped nucleus. Also in the 5-day water-deprived group, mitochondria was decreased in number and junctins were disappered. Endoplasmic reticulum, Golgi complex did not show changes after water-deprivation. In this results, we can conclude that dopamine are involved in the water metabolism in mongolian gerbil, and mongolian gerbil could be used as an animal model for the researches of water deprivation.

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Health Medical Center Utilization Pattern and Its Related Factors among the Rural Inhabitants (농촌지역(農村地域) 주민(住民)들의 보건의료원(保健醫療院) 이용양상(利用樣相)과 관련요인(關聯要因))

  • Hwang, Byung-Deog;Park, Jae-Yong
    • Journal of agricultural medicine and community health
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    • v.18 no.1
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    • pp.77-91
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    • 1993
  • This study was conducted to assess health medical center utilization pattern and its related factors among the rural inhabitants for the purpose of contribution to establishment of health medical center institutions. A questionnaire survey was carried out for object of 3,754 population of three primary school and three middle school student's parents (total 832 household) in Kyungbook Ulchin Gun rural area from 24 to 28 September, 1990. The summarized result are as follows, Respondents are 60.3% in male, 39.7% in female and 30-40s 81.3% in age, high school graduates 40.3% in education level and a regional medical insurance scheme in 44.1% in forms of health insurance. Recognition for health medical center was showed higher according to high educational, high income level, and short distance for location of health medical center of respondents (p < 0.01). Recognition for health medical center services was showed higher about care of medicaid in medical treatment services and higher preventive vaccination in health prevention services by respondents. Utilization rates of health medical center by out-patient care and preventive care service were 11.1 and 4.5 per 100 persons by year, but admission utilization rate was 34.6 per 10.000 persons by year. Motivations of health medical center utilization were showed a good care(45.7%), a good drugs(45.2%), and nearby health medical center(42.9%). In comparison health service levels of health medical center with general clinic was better (16.3%), similar(38.7%), 7(19.0%), and worse(19.0%) in view of health medical center utilizators. Inconvinience about health medical center utilized was the most higher longtime waiting, the next was limited utilization times. Transportation utilited were on foot(55%), by bus(35.5%), and so on. As mentioned above, there are many inhabitants who less understanding and less acknowledgement about health medical center and even mistake health center for health medical center. Therefore, there must be more information about health medical center. For higher utilization of health medical center, there must be considered expansion of health equipment, facilities, accomplishment with reinforcement of health staffs and efficiency management.

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A Study on Public Health Doctors' Participation in District Public Health Program of Health Sub-centers in Korea (보건지소 공중보건의사의 지역보건사업 참여 실태)

  • Lee, Jae-Chun;Park, Yong-Moon;Ahn, Song-Vogue;Lee, Hae-Young;Hwang, Jin-Won
    • Journal of agricultural medicine and community health
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    • v.28 no.1
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    • pp.53-66
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    • 2003
  • Objectives: To investigate the state of medical care around health sub-centers, public health doctors' participation and opinion in the process of district public health programs. Methods: The study included 1,036 public health doctors who worked at health sub-center all over the country. The data were collected for Feb, 2002 using self-administered questionnaire by mail. Results: One or two doctors were working at health sub-center and 33.5% of health sub-centers was located in the region of the separation of prescription and dispensing. There were another medical facilities in 45.9% of the administrative district(eup or myon) where health sub-centers were located. The count of medical utilization went down to 14.8${\pm}$14.8 per a day in Nov, 2001 from 18.0${\pm}$15.6 in May, 2000, and the decline was much more in the region of the separation of prescription and dispensing. Among public health programs in health sub-centers, public health doctors participated mostly in preliminary medical examination for vaccination and least in health education. They participated in implementation rather than planning or evaluation of health program. Over a half of public health doctors were found to be positive that health programs implemented in their health sub-centers would promote the level of health in community people and they were willing to participate in district public health program if community people were in need. Conclusions: Recently health sub-centers are required to turn into health promotion facilities rather than medical practice facilities. Health program in health sub-centers will be advanced in both quality and quantity by turning the role of public health doctors who have provided medical services mainly into managing health program. Persistent education about managing health program and the policy to motivate participation in health program should be provided for public health doctors.

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Analysis of Micro-Sedimentary Structure Characteristics Using Ultra-High Resolution UAV Imagery: Hwangdo Tidal Flat, South Korea (초고해상도 무인항공기 영상을 이용한 한국 황도 갯벌의 미세 퇴적 구조 특성 분석)

  • Minju Kim;Won-Kyung Baek;Hoi Soo Jung;Joo-Hyung Ryu
    • Korean Journal of Remote Sensing
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    • v.40 no.3
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    • pp.295-305
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    • 2024
  • This study aims to analyze the micro-sedimentary structures of the Hwangdo tidal flats using ultra-high resolution unmanned aerial vehicle (UAV) data. Tidal flats, located in the transitional area between land and sea, constantly change due to tidal activities and provide a unique environment important for understanding sedimentary processes and environmental conditions. Traditional field observation methods are limited in spatial and temporal coverage, and existing satellite imagery does not provide sufficient resolution to study micro-sedimentary structures. To overcome these limitations, high-resolution images of the Hwangdo tidal flats in Chungcheongnam-do were acquired using UAVs. This area has experienced significant changes in its sedimentary environment due to coastal development projects such as sea wall construction. From May 17 to 18, 2022, sediment samples were collected from 91 points during field surveys and 25 in-situ points were intensively analyzed. UAV data with a spatial resolution of approximately 0.9 mm allowed identifying and extracting parameters related to micro-sedimentary structures. For mud cracks, the length of the major axis of the polygons was extracted, and the wavelength and ripple symmetry index were extracted for ripple marks. The results of the study showed that in areas with mud content above 80%, mud cracks formed at an average major axis length of 37.3 cm. In regions with sand content above 60%, ripples with an average wavelength of 8 cm and a ripple symmetry index of 2.0 were formed. This study demonstrated that micro-sedimentary structures of tidal flats can be effectively analyzed using ultra-high resolution UAV data without field surveys. This highlights the potential of UAV technology as an important tool in environmental monitoring and coastal management and shows its usefulness in the study of sedimentary structures. In addition, the results of this study are expected to serve as baseline data for more accurate sedimentary facies classification.