• Title/Summary/Keyword: 대물 렌즈

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Construction of Confocal Optical Microscope based on Resonant Scanning Mirror (공진 스캔 미러를 이용한 공초점 광학 현미경 구현)

  • Kim, Dong-Uk;Song, Ho-Seong;Song, U-Seop;Kim, Deok-Yeong
    • Proceedings of the Optical Society of Korea Conference
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    • 2008.07a
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    • pp.275-276
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    • 2008
  • 광 검출기 앞에 미세한 구멍을 대물렌즈의 켤레 초점면에 놓음으로써 초점에 정확하게 일치하는 빛만 검출하는 공초점 광학 현미경은 마이크로미터 이하의 불투명한 샘플 표면, 구조뿐만 아니라 투명한 샘플인 경우 절단 없이 선택적으로 단면을 관찰하는데 사용된다. 이런 장점 때문에 특정 원자나 분자에 인위적으로 염색 시킬 수 있는 형광물질을 사용하여 세포의 기관 구조나 세포 기관간의 활동을 3차원적으로 관찰하는 생물학연구에서도 공초점 광학 현미경이 사용된다. 본 논문에서는 공진 스캔 미러를 이용하여 공초점 광학 현미경을 구현 하고자 한다.

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Surface profiling by the phase shifting method in fiber-optical confocal scanning interference microscopes (광섬유 공초점 간섭 현미경과 위상 변위법을 결합한 표면 검색)

  • 김대찬;이승걸
    • Korean Journal of Optics and Photonics
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    • v.10 no.3
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    • pp.201-207
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    • 1999
  • The fiber-optical confocal scanning interference microscope with a simple configuration was constructed with a 4-port fiber-optic coupler, and the new method based on the phase shifting method was proposed for surface profiling by the system. In the method, the height of a specimen was determined from the phase of confocal beam. It was verified experimentally that the method was applicable to even the confocal interference microscope with a long-wavelength source and a low NA objective, and that the scanning time could be drastically reduced compared with the conventional method. Finally, it was found that our method is less sensitive to the variation of surface reflectivity than the conventional method.

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Manufacture of Optical Probe Using $CO_2$ Laser ($CO_2$ 레이저를 이용한 광섬유 탐침(Optical probe)의 제작)

  • Shin, Youl;Kim, Young-Ill;HwangBo, Soung
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.07b
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    • pp.1058-1061
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    • 2004
  • 미세 소자를 관측, 가공 및 분석하기 위해 사용되는 기존 광학현미경은 빛으로 물체를 관측하므로 대물렌즈 (Object lens)에서의 회절한계 때문에 분해능의 있으므로 매우 뽀족한 탐침(Probe)을 시료의 표면에 근접시킨 후 표면을 주사하여 이미지를 얻는 방법이 개발되어 최근에는 Optical Fiber를 이용하여 fiber 끝단을 nano-scale 정도로 첨예화시키는 기술이 개발되었다. 이러한 광섬유 탐침은 구경의 직경이 작을수록 높은 분해능을 얻을 수 있으므로 광섬유 탐침의 제작 공정 확립은 매우 중요하다. 그 중에서 대표적인 방법이 $CO_2$ 레이저를 이용하여 가열한 후 인장 하는 방법 (Heating and Pulling)이 있다. 그래서 본 연구에서는 $CO_2$ 레이저를 이용하여 100nm 정도의 팁 반경을 갖는 뽀족한 탐침을 제작하고자 한다.

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Study on Golf Range Finder by using Biprism Theory of Keratometer (케라토미터의 바이프리즘 원리를 이용한 골프거리측정기에 관한 연구)

  • Cha, Jung-Won
    • Journal of Korean Ophthalmic Optics Society
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    • v.18 no.4
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    • pp.495-501
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    • 2013
  • Purpose: This study was to develop golf range finder which could measure exact distance from golfer to flag by using telescope and biprism. Methods: Golf range finder was made that included telescope and biprism which could move in the telescope, and an accuracy of the range finder was analyzed in distance range 20~200 m. Results: An equation was made for measuring distance which was related at position of biprism, and we could measure distance successfully by using developed golf range finder. Biprism of 2 ${\Delta}$ was useful to measure in short distance but it had bad accuracy in long distance, and 0.5 ${\Delta}$ was more useful in long distance. Image got worse when the biprism approch to focal point of objective lens for measurement in long distance, so we developed multi-layer biprism to prevent the weak point. Conclusions: Image stabilization by biprism for measuring distance was confirmed. Even if hand was moving, the distance could be measured well by using biprism, and we could find that the induced equation for distance measurement was useful. We found that multi-layer biprism, which is was new developed, was more useful than general biprism when the biprism approched to focal point of objective lens for measurement in long distance.

Three-Dimensional Approaches in Histopathological Tissue Clearing System (조직투명화 기술을 통한 3차원적 접근)

  • Lee, Tae Bok;Lee, Jaewang;Jun, Jin Hyun
    • Korean Journal of Clinical Laboratory Science
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    • v.52 no.1
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    • pp.1-17
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    • 2020
  • Three-dimensional microscopic approaches in histopathology display multiplex properties that present puzzling questions for specimens as related to their comprehensive volumetric information. This information includes spatial distribution of molecules, three-dimensional co-localization, structural formation and whole data set that cannot be determined by two-dimensional section slides due to the inevitable loss of spatial information. Advancement of optical instruments such as two-photon microscopy and high performance objectives with motorized correction collars have narrowed the gap between optical theories and the actual reality of deep tissue imaging. However, the benefits gained by a prolonged working distance, two-photon laser and optimized beam alignment are inevitably diminished because of the light scattering phenomenon that is deeply related to the refractive index mismatch between each cellular component and the surrounding medium. From the first approaches with simple crude refractive index matching techniques to the recent cutting-edge integrated tissue clearing methods, an achievement of transparency without morphological denaturation and eradication of natural and fixation-induced nonspecific autofluorescence out of real signal are key factors to determine the perfection of tissue clearing and the immunofluorescent staining for high contrast images. When performing integrated laboratory workflow of tissue for processing frozen and formalin-fixed tissues, clear lipid-exchanged acrylamide-hybridized rigid imaging/immunostaining/in situ hybridization-compatible tissue hydrogel (CLARITY), an equipment-based tissue clearing method, is compatible with routine procedures in a histopathology laboratory.

The property of WC(Co 0.5%) ultra precision turning for optical pick-up objective lens molding press for optical infomation storing(I) (광정보저장용 광픽업 대물렌즈 성형용 초경합금 (Co 0.5%) 초정밀절삭 특성(I))

  • Kim, Min-Jae;Lee, Jun-Key;Hwang, Yeon;Kim, Hye-Jeong;Kim, Jeong-Ho
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.11a
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    • pp.178-178
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    • 2009
  • High-density optical information storing equipment, which is using Blu-ray, is the next generation information storing equipment that has about form six times to thirty-five times capacities. and high-density optical information storing equipment uses high NA(Numerical Aperture) aspheric glass objective lens as optical pick-up equipment to record and recognize high-density date. Generally this objective lens is developed and produced through a way of GMP(Glass Molding Press) that uses molding core that is performde by Ultra precision grinding, but grinding performing that has high-accuracy is very difficult because objective lens form is high NA. In this research, we preformed Ultra precision turning, using single crystal diamond bite, about WC(Co 0.5%), sintering brittleness material that is used molding core's material for GMP. and we confirmed aspheric glass lens compression of deformities molding core's Ultra precision turning possibility by measuring surface roughness(Ra) and processing surface's condition.

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MOCVD를 이용한 삼차원 무형광체 백색 발광다이오드 제작 및 분석

  • Im, Seung-Hyeok;Go, Yeong-Ho;Rodriguez, Christophe;Gong, Su-Hyeon;Jo, Yong-Hun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.238-238
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    • 2016
  • 백색 발광다이오드(LED)는 기존 조명(백열등, 형광등)에 비하여 월등히 에너지를 절약할 수 있어 이미 상업적, 정책적으로 교체가 진행 중이다. 현재의 백색 LED를 만들기 위해서는 필연적으로 형광체를 사용해야 한다. 그러나 이 형광체에 의한 (a)Stocks 에너지변환에 의한 효율감소, (b)높은 공정비용, (c) 열적 안정성 저하를 피할 수 없다. 우리는 유기금속화학증착(MOCVD)과 선택적성장(selective-area epitaxial growth)방법을 이용하여 형광체를 쓰지 않고 3차원 구조체를 이용하여 백색 LED를 제작하여 전기구동하였고 전류의 세기를 변화하여도 지속적으로 동일한 백색광을 유지함을 보였다. 광학적 분석(cathodoluminescence)과 구조적 분석(scanning electron microscope, transmission electron microscope)을 진행하여 구조를 살피고 백색 발광의 원인을 분석하였다. 또한, 고배율 대물렌즈를 사용한 공간분해 광학적 분석실험(photoluminescence와 electroluminescence 데이터를 비교)으로부터 국소적 운반자의 주입효율을 분석하는 방법을 고안하여 실험하였다.[1] 향후 이 방법은 3차원 구조체 LED뿐 아니라 2차원 LED에도 응용하여 LED의 주입효율을 분석하는데 유용하게 사용될 수 있을 것이라 기대된다.

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Ultra Precision Machining of Optical Pick-up Aspheric Glass Objective Lens Molding Press Core for Optical Information Storing (I) (광정보저장용 광픽업 대물렌즈 성형용 코어 초정밀 형상가공 (I))

  • Kim, Min-Jae;Lee, Jun-Key;Hwang, Yeon;Kim, Hye-Jeong;Kim, Jeong-Ho
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.06a
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    • pp.290-290
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    • 2009
  • Blu-ray Disk, the next generation optical information storing equipment used Blu-ray is the next generation leading storing equipment that has capacity is about from six times to thirty-five times bigger than the existing CDs, DVDs. Especially, we need elaborate optical pick-Up equipment to record and recognize detailed date. Moreover, Blu-ray disk has so narrow track-pitch so it is used high NA(Numerical Aperture) aspheric glass objective lens. In this research, we processed optical pick-up aspheric glass objective lens molding press core by parallel grinding method with ultra precision machining and mold core surface measured form accuracy(PV), surface roughness(Ra).

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Dynamic characteristics of optical pickup actuator with a newly designed electromagnetic circuit (신 자기회로가 적용된 광픽업 액추에이터의 동특성)

  • Park, Kwan-Woo;Kim, Jae-Eun;Jung, Jae-Hyun;Lee, Kyung-Taek;Ko, Eui-Seok;Hoon, Min-Byung
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2008.11a
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    • pp.512-514
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    • 2008
  • In this paper, a new electromagnetic circuit is proposed for an optical pickup actuator with high sensitivity. Contrary to those of conventional actuators, the proposed circuit has two focusing coils which are diagonally placed at the front and rear of a moving part. The configuration which makes the effective length of the focusing coil longer and the moving part lighter, is helpful in increasing the sensitivity of the actuator. However, the asymmetry of the moving part by two focusing coils causes flexible node vibrations in quite low frequency range. This paper shows that the design modification of the moving part for the reconfiguration of mass moment of inertia can reduce the mode vibrations.

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Theoretical Calculation on Radiation Patterns of Epi-signal in CARS Microscopy (간섭성 반스톡스 라만 산란 현미경 후방 신호지 방사패턴에 관한 이론계산 연구)

  • Yoo, Yong-Shim;Cho, Hyuck
    • Korean Journal of Optics and Photonics
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    • v.18 no.4
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    • pp.286-291
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    • 2007
  • We theoretically investigated the far-field radiation pattern of epi-signal from a polystyrene sphere in coherent anti-Stokes Raman scattering (CARS) microscopy with an objective lens of high numerical aperture. We calculated the field distribution of the incident laser beams under the tight-focusing condition and the far-field radiation pattern through coherent addition of radiation from the nonlinear polarizations (Hertzian dipoles) as the origin of CARS signal generation. The epi-radiation patterns for polystyrene spheres of different diameters are calculated, and the pattern of a sphere is also compared with that of a shell fer a diameter of 1100 nm. We finally show how the radiation pattern of the polystyrene sphere changes as the center of the sphere shifts from the focus of the beam.