• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.63-70
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• 2012

This study was conducted to investigated the distribution, ecological character and life cycle of Black Soldier Fly(BSF), Hermetia illucens, to determine effects of BSF on composing ability to the livestock feces. The distribution of BSF was defined in all parts of the country in Korea. Its main habitat was found to be areas near cattle sheds, feces sheds, living waste dump grounds, and food waste dump grounds. Excellent species of the bio-conversion using the livestock feces were selected out of two kinds of solider flies(Hermetia illucens and Ptecticus tenebrifer). And effects of BSF on composing ability to the livestock feces were pig feces 0.5 g, cattle feces 0.43 g and chicken feces 0.3 g per a larvae of BSF. In the examination of the ability of BSF to decompose food waste, volume of the livestock feces decreased by weight pig feces 77, cattle feces 83.62 and chicken feces 80%. Observed characteristics of BSF larvae and pupae stage were by developmental stage may be summarized as follows: size and length of larvae and pupae of BSF were higher than in pig feces and mixed sawdust treatments. In order to develop artificial indoor rearing techniques, The pupation and emergence rate were not visible the difference but important factor of the mating and egg-laying was a sun light. And it was visible the difference in mating ratio. Mating rate of H. illucens by seasonal change was the highest from May to July. Mating mostly occurred between 10 : 00 and 12 : 00 during which light intensity is high. As the egg-laying medium for artificial egg collection, calf feed and food waste were most effective. The ratio of egg-laying medium is waste food : sawdust(1 : 1) in volume. For egg-laying materials, flower foam and wood with holes were used; holes sized 3 ~ 5 mm in diameter, 7 ~ 10 mm in depth were most preferred for egg-laying.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.3 no.1
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• pp.52-64
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• 1970

Fingerlings hatched from the eggs of the puffer, Fugu rubripes, which were spawned on May 21, 1969, and were cultivated. The results of their growth during 150 days, until October 25th, are summarized as follows: 1. The eggs began to hatch after 163 hours, at a water temperature of 15.9 to $17.4^{\circ}C$, and the hatching rate was $61.56\%$. 2. They reached the post-larval stage 6 to 7 days after hatching, and at this time a high mortality occurred. The mortality rate was 57.26 to $68.0\%$. 3. Sixteen days after hatching some of the larger fingerlings (6.7mm in total length) began attacking some of the smaller ones (4.6mm in to total length). 4. Twenty five to twenty eight days after hatching, the fish changed their food, and at this time a second high mortality occured. The total mortality rate amounted to 90.7 to $90.9\%$ of the total hatch. 5. After the fingerling stage. cannibalism occurs. The fish usually attack the caudal part of other fingerlings. It occurs regardless of body length and when the food supply is short. 6. The food coeffiicient at the age of 46 days (when body length is 53 to 68 mm) was 5.5 for short-necked clams, 8.5 for earth-worms, and 8.7 for fishes. 7. A third hish mortality occurred 53 to 63 days after hatching, the total mortality amounting to 95.76 to $97.34\%$, and the main cause of the mortality was found to be rickets resulting from nutritional deficiency 8. The growth rates were as follows: 2.68mm just after hatching, 3.84mm at the age of 10 days; 7.96mm after 25 days; 20.96mm or 130mg after 40 days; 73.68mm or 9.06g after 80 days: and 123mm or 31.8g after 150 days. 9. The water temperature during the above period was 15.7 to $28.4^{\circ}C$ with an average of $22.10^{\circ}C$ and the salinity was 25.53 to $34.50\%$ with an average of $32.07\%$, 10. The young of this species could endure well a wide range and sudden rise in salinity, and could survive easily when the salinity suddenly fell to $5\%$, but a considerable mortality occured when it fell to $3\%$. 11. When the fish were tranferred directly to fresh water from normal sea water they died out in 9 hours and 40minutes. However, when transferred from water of $5\%$ salinity at which they were reared for 54 days, they survived for 60 hours and 40 mimutes longer than in the former case.

• Korean Journal of Environmental Biology
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• v.41 no.3
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• pp.325-334
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• 2023

The invasive red swamp crayfish, Procambarus clarkii, is native to south-central United States and northeastern Mexico. Recently, it has been being spreading in the wild in South Korea. However, its primary sources, introduction routes, establishment, and expansion in South Korea remain unclear. Here, we analyzed genetic diversity and population genetic structures of its domestic natural populations during early invasion, commercial stock from local aquaria (a suspected introduction source), and original United States population using mitochondrial COI gene sequences for 267 individuals and eight microsatellite markers for 158 individuals. Natural and commercial populations of P. clarkii showed reduced genetic diversity (e.g., haplotype diversity and allelic richness). The highest genetic diversity was observed in one original source population based on both genetic markers. Despite a large number of individuals in commercial aquaria, we detected remarkably low genetic diversity and only three haplotypes among 226 individuals, suggesting an inbred population likely originating from a small founder group. Additionally, the low genetic diversity in the natural population indicates a small effective population size during early establishment of P. clarkii in South Korea. Interestingly, genetic differentiation between natural populations and the United States population was lower than that between natural populations and aquarium populations. This suggests that various genetic types from the United States likely have entered different domestic aquariums, leading to distinct natural populations through separate pathways. Results of our study will provide an insight on the level of genetic divergence and population differentiation during the initial stage of invasion of non-indigenous species into new environments.

• Journal of Aquaculture
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• v.21 no.4
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• pp.203-212
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• 2008

A nutritional demand of oyster, Crassostrea gigas larva as part of research for improving of utilization of microalgae being used for the artificial oyster seed production. The change of body growth and biochemical compositions of larvae were investigated during larvae rearing in hatchery. The larvae were cultured in 60 M/T tank and fed mixture 6 different phytoplankton species, Isochrysis galbana (30%), Cheatoceros gracilis (20%), Pavlova lutheri (20%), Phaeodactylum triconutum (10%), Nannochryis oculata (10%) and Tetraselmis tetrathele (10%). The initial feeding amount was $0.3{\times}10^4cells/mL$ at three times a day to D-shaped larva and the feeding amount had been increased 30% gradually every two day since the larvae were raising. The larvae were developed from D shape to pediveliger stage for 12 days. The daily growth of shell length and hight were $5.8{\sim}30.8\;{\mu}m$ and $8.7{\sim}31.4\;{\mu}m$, respectively and weight gains were changed from D shape to pediveliger as follow: wet weight was $0.52{\sim}15.0\;{\mu}g/larva$, dry weight was $0.2{\sim}6.5\;{\mu}g/larva$, and ash free dry weight was $0.1{\sim}8.5\;{\mu}g/larva$. The larvae growth pattern shown a logarithmic phase from D shape to umbone stage but after that stage shown a exponential growth aspect. The crude protein, crude lipid and nitrogen free extract (NFE) of larvae during rearing periods were analyzed as $6.1{\sim}10.6%$, $0.6{\sim}1.1%$ and 1.0-2.7%, respectively. And the total amino acid contents of the larvae during rearing periods were in order as glutamic acid $1.26{\sim}2.24%$, aspartic acid $0.97{\sim}1.70%$, and methionine $0.12{\sim}0.33%$. Of the total fatty acid in the analyzed larvae, the saturated fatty acid (SSAFA) was decreased from 54.3% (D shaped larvae) to 17.1 % (pediveliger) as larvae development but the total mono-unsaturated fatty acid (${\Sigma}MOFA$) and Poly-unsaturated fatty acid (${\Sigma}PUFA$) were increased from 29.9% and 7.8% to 40.6% and 45.6%, respectively. By the way the each fatty acid of the larvae were composed of palmitic acid $9.89{\sim}36.95%$, oleic acid $12.17{\sim}32.29%$, linoleic acid $1.96{\sim}33.55%$, EPA $2.17{\sim}11.58%$ and DHA $1.95{\sim}4.51%$. As a result of this study, the larvae of oyster were demanded a various nutrients for healthy growth and the feeding control, expecially after umbone stage larvae are a rapidly growing time, is very important for success of artificial seed production.

• Journal of Aquaculture
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• v.2 no.2
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• pp.91-106
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• 1989

Chlorella has been used as a very useful food for rearing rotifer which is an important live food for early stages of fish and crustancean larvae. But Chlorella does not grow well in higher or lower temperature such as during summer or winter season in this country. Therefore, cooling or heating facilities are needed for Chlorella culture during summer or winter, but it costs too much for the commercial scale fish farmers. To solve this problem, the growth rates of 34 different species of phytoplanktons were examined at the various levels of temperatures, salinites and light intensities to select suitable species as the food for rotifers for summer and that for winter. After the suitable species were selected, growth comparisons of rotifer groups which were fed the selected species of phytoplanktons against rotifer group fed Chlorella as a control were done. Fatty acid compositions of the selected phytoplanktons and rotifer groups which were fed these selected phytoplanktons were examined. It was revealed that Nannnochioris oculata was optimum for rotifers in summer season and Phaeodactylum tricornutum was suitable for that in winter season. The optimum temperature, salinity and light intensity for former phytoplankton were $28^{\circ}C$, $33\%_{\circ}$ and 5,000 lux and those for later were $10^{\circ}C$, $30\%_{\circ}$ and 8,000 lux, respectively. In the higher temperature condition, the growth of N. oculata fed rotifer group was better than Chlorella ellipsoidea fed group. In the lower temperature condition, however, the growth of Chlorella fed rotifer group was slightly better than P. tricornutum fed group. Between two selected phytoplanktons, N. oculata has the highest content of linolenic acid (18 : 3 $\omega$ - 3, $\omega$ - 6) which is essential fatty acid for marine fish larvae. A rotifer group which was fed this plankton also showed the highest linolenic acid content among the other rotifer groups.

• Korean journal of applied entomology
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• v.44 no.1 s.138
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• pp.61-65
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• 2005

Egg-forming and preservation methods for the indoor-rearing system of the emma field cricket, Teleogryllus emma, were investigated. The oviposition preference experiment on different mats, soil(natural oviposition mat) and $oasis^{\circledR}$(artificial oviposition mat), showed that T. emma preferred to oviposit on the $oasis^{\circledR}$. On the other hand, $oasis^{\circledR}$ had more inactive number of eggs laid per female and hatchability than another. However, the number of eggs laid per female and hatchability within 50 days after emergence were better than soil. The experiment on the hatching of the eggs showed that eggs could be stocked at $10^{\circ}C$ for 40 days with 14 day pre-period after laying, representing $62.1\%$ hatchability, when considering generation shortening. On the other hand, When it had been kept in cold storage for 90 days at $7.5^{\circ}C$ with 14 day pre-period after laying it, appeared to be the most suitable for long period-storage method by hatchability $65.1\%$.

• Journal of fish pathology
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• v.4 no.2
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• pp.79-85
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• 1991

In Feb., 1990 an epizootic disease to masu salmon fry, Oncorhynchus masou cultured at the hatchery of rainbow trout in Samchok, Kwangwon-do have broken out and induced high mortality over 70%. Externally, the diseased fish showed dark discoloration, abdominal distension with ascites, slight exophthalmus and fecal casts. Internally, the gill and the liver of diseased fish were edematous and pale, and the stomach of moribund fish contained the milkish fluid. Microscopically there was extensive necrosis of the hematopoietic tissue in kidney and spleen, and scattered necrosis of pancreas, liver and lateral muscle. Especially, the necrosis of lamina propria, muscle layer and tela submucosa in the digestive tract known as the typical signs associated with infectious hematopoietic necrosis virus was seen clearly.

• Journal of the Korea Organic Resources Recycling Association
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• v.22 no.4
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• pp.11-20
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• 2014

Recently, manure emissions associated with the numerical increment of the domestic hog-breeding have increased, but it has been banned from ocean dumping by the United Nations Convention in 2012. To find out the site for spraying in large quantity of liquid manure fertilizer, we selected one grass plantation, which has the largest amount of grass production, in Jangsung, South Korea. We spread not only chemical fertilizer but also liquid manure fertilizer on the zoysiagrass plantation and researched the effect on the grass growth and development, soil chemical property, and seepage water of dike. As the test results, spread by compounding chemical fertilizer and liquid manure fertilizer and it is possible to substitute for some chemical fertilizer in terms of nutrients accumulation and the grass growth and development. Additionally, it gives less effects to soil chemical property and is efficacious to effective microorganism growth of soil. Therefore, adding liquid manure fertilizer will lead to reduce almost all the amount of chemical fertilizer usage. It is also expected to help for livestock manure management and to influence reducing the production cost of the grass plantation. However, to spread liquid manure fertilizer contaminates water system. For this reason, monitoring continuously, we need to find out improvement plans of the fertilization method in order to increase the usage of liquid manure fertilizer.

• Journal of Aquaculture
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• v.17 no.3
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• pp.180-186
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• 2004

This study was carried out to obtain the fundamental data for the mass seedling production of grunt, Hapalogenys nitens in terms of the natural spawning and some characteristics of the eggs spawned. The wild grunt were reared at indoor tanks for three years. The adults spawners were 34.0∼44.0 cm (38.6$\pm$4.0 cm, n=7) in total length, 1.00∼2.23 kg (1.62$\pm$0.50 kg, n=7) in body weight. Spawning were observed 9 times from September 22 to October 1, 2000 and 37 times from August 22 to October 3, 2001, with a water temperature range of 19.8$\pm$28.5$^{\circ}C$. The total number of eggs collected was 2.29${\times}$10$^{7}$ (1.7${\times}$10$^{3}$/ml). The relative proportion of floating eggs to total eggs was 41.7%. The fertilization rate of floating eggs was ranged between 85.0 and 99.9% and the hatching rate was ranged between 2.9 and 93.0%. Fertilized eggs were buoyant and spherical in shape, and were 0.85∼0.98 mm in diameter. Each egg contained 1-5 oil globules which were, 0.18∼0.25 mm in diameter. The incubation time from fertilization to blastodisc formation was 10 minutes, to blastula was 3 hours, and to the hatched larvae at 26$^{\circ}C$ was 20 hours 30 minutes. The newly hatched larvae attained total length of 1.81$\pm$0.18 mm. The time required from fertilization to hatching was 31∼34 hours at 23$^{\circ}C$ and 17∼20 hours at 29$^{\circ}C$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.5 no.2
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• pp.45-49
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• 1972

The following are some results obtained from a series of experiments in rotifer culture and its usage for the food of tiny fish fry: 1, Outdoor concrete ponds, each being $16m^2$, were used to culture the rotifers, Brachionus calyciflorus, and Filinia longiseta. Brachionus calyciflorus usually attained the population of about 100 individuals per ml of pond water. Dipterex was usually applied to control Daphni,a and other crustaceans that generally appear and feed on rotifers. A concentration of 0.16 to 0.2 ppm in the pond water was sufficiently effective to control these natural enimies of rotifers. Poultry dung was very effectively used to multiplicate rotifers. The fertilization ratio was about 8 kg each pond with 30cm depth of water. 2. The tiny rotifer, Filinia longiseta attained a very high population density of about 1,000 individuals per ml of pond water, but they were very sensitive to dipterex, and for this aspect future investigation may be needed. 3. In the outdoor ponds, the multiplication of rotifers significantly decreased when the water temperature falls to about $20^{\circ}C$ in autumn. 4. In the laboratory room, unicellular planktonic algae such as Scenedesmus or Chlorella, as the food of rotifers, were collected from the outdoor ponds by dipping them together with water, and were effectively used for the culture of Brachionus calyciflorus. If the planktonic algae are cultured in specially designed containers, the sun-light would be the most effective means as the source of light. 5. Brachionus calyciflorus cultured in the outdoor ponds by the dipterex controlled method was highly efficient to rear the early fry of marble gourami. The dipterex content mixed in the water to control the crustacean emmies of rotifers sieved no harm to the gourami fish fry.