• Korean Journal of Food Science and Technology
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• v.31 no.2
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• pp.482-487
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• 1999

Periodate-oxidized soluble starch and maltohexaose reacted with ${\alpha}-NH_2$ group of free amino acids and ${\varepsilon}-NH_2$ group of peptidyl lysine. The result shows that periodate-oxidized soluble starch and maltooligosaccharides reacted with protein and formed Schiff base between CHO group of oxidized sugar and ${\varepsilon}-NH_2$ group of surface lysine of protein molecule. Carbon and hydrogen composition of sweet potato ${\beta}-amylase$ modified with oxidized soluble starch increased and it's nitrogen composition decreased. Carbohydrate contents of sweet potato ${\beta}-amylase$ modified with oxidized soluble starch were 13.2% (pentamer), 13.4% (monomer), and with oxidized maltohexaose were 9.7% (pentamer), 9.3% (monomer) by $phenol-H_2SO_4$ method. Alpha-amino group of N-terminal, and ${\varepsilon}-NH_2$ group of lysine, of sweet potato ${\beta}-amylase$ were reacted with oxidized soluble starch by dinitrophenylation were 70% (pentamer), 73% (monomer) and 33% (pentamer), 26% (monomer), respectively, in comparison with native enzyme.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2022.06a
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• pp.426-428
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• 2022

해상 교통량을 정량적으로 평가하고 추출하기 위한 방법으로 선박 AIS 데이터 기반의 밀집도 분석을 활용하고 있다. 밀집도는 단위시간 당 단위면적에 분포하는 선박 통항량을 계산한 것으로, 일반적으로 그리드 셀 내에 존재하는 선박 항적 포인트 개수, 항적도 라인 길이, 선박 척수 등을 계산한 밀집도 분석 방법과 커널 밀도 추정(Kernel Density Estimation) 방법 등이 있다. 하지만, AIS 데이터의 특징상 선박 속력에 따라 수신 주기가 다르기 때문에 항적이 등간격으로 나타나지 않는 문제점이 있으며, 선박의 이동과 시간의 속성으로 인해 각각의 밀집도 분석 방법은 한계점이 존재한다. 따라서 본 연구에서는 실측 AIS 데이터를 이용하여 다양한 방법의 선박 밀집도 분석을 수행하고 이를 비교하였다. 그 결과, 항적도 라인 길이에 의한 밀집도 분석이 가장 정량적인 방법으로 나타났으며 이를 통항 척수로 변환할 수 있는 선박 밀집도 분석을 개선방안으로 제안한다.

• Korean Journal of Food Science and Technology
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• v.21 no.4
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• pp.515-520
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• 1989

The composition of lipid class and fatty acid of free lipids(FL) as non-starch lipid and bound lipids(BL) as starch-lipid extracted from starch In naked barley(Hordeum vulgare L.) was investigated with the chromatographic procedures. FL were extracted from barley starch by petroleum ether(PE) and then BL were reextracted from PE extracted starch by the solvent systems of water-saturated butanol (WSB) at $25^{\circ}C$ and at $95^{\circ}C$ respectively. The contents of neutral lipid(NL), glycolipids(GL) and phospholipids(PL) in FL were 69.9%, 27.3%, and 2.8%, on the other hand those of BL were 34.9-54.6%, 30-45.5% and 15.4-19.6%, respectively. The identified components of NL in starch-lipid were triglycerides (70.4-82.4%), free fatty acid (8.4-26.2%), esterified sterols and free sterols, and also the major GL in starch-lipid was monogalactos-yldiglycerides(87.2-91.1%). Of the PL in FL and BL, diphosphatidyl glycerols, lysophosphatidyl choline, phosphatidyl choline & phosphatidyl serine were the major components. The predominent fatty acids found in NL, GL and PL of barley starch were palmitic acid and linoleic acid, and also myristic, stearic, oleic, linolenic acids were determined.

• Journal of the Korean Society of Food Science and Nutrition
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• v.17 no.3
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• pp.262-268
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• 1988

Total lipids of Korean black Soybean were extracted, purified and fractionated into three lipid classes (Neutral lipid, NL; glycolipid, GL;phospholipid, PL) and the composition of lipid class and fatty acid were studied. Total lipids of black soybean consisted of 88.5% NL, 2.5% GL and-9.0% PL. In the NL, triglycerides were predominant(80.4%) and also sterol esters, hydrocarbons, diglycerides, free fatty acids and sterols were identified. The major component of GL were monogalactosyl diglycerides and esterified steryl glycosides, and then cerebrosides, steryl glycosides, digalactosyl diglycerides were also found. As major component of the PL, phosphatidyl ethanolamine and phosphatidyl choline were observed, other compounds such as phosphatidyl inositol, phosphatidyl serine and lysophosphatidyl choline were also determined. The major fatty acids in the NL and GL were linoleic acid, oleic acid and palmitic acid, however, PL contained higher relative content of palmitic acid and lower level of oleic acid compared with those of NL and GL.

• Journal of Plant Biotechnology
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• v.33 no.4
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• pp.283-288
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• 2006

Discrimination of 5 rice cultivars (Sangjubyeo , Dongjinbyeo Simbaekbyeo , Hwamanbyeo , and Simbaek-hetero ) using metabolic profiling was carried out. Whole cell extracts from each cultivar were subjected to $^1H$ NMR spectroscopy. When spectral data were analyzed by principal component analysis, 5 cultivars were clustered into 3 groups: SJ, DJ + SB, and HM + SH. Thecultivars showed great difference in carbohydrate region of $^1H$ NMR spectra, suggesting that qualitative and quantitative differences in carbohydrate compounds play a major role in discrimination of the cultivars. In addition, it was readily possible to determine relative quantification of major carbohydrates including sucrose, glucose, maltose from spectral data of the cultivars. SJ showed 2 to 4 times higher content of maltose than the other rice cultivars. Overall results indicate that metabolic discrimination of rice cultivars using $^1H$ NMR spectroscopy combined by multivariate statistical analysis can be used for rapid discrimination of numerous rice cultivars and simple quantitative analysis system of major carbohydrate compounds in rice grains.

• Applied Biological Chemistry
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• v.5
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• pp.39-42
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• 1964

1) The Moisture and glycogen contents of salted-oyster (Zin Suk Hwa Jut) were much less than those of raw oyster, while the free sugar content was dominent. 2) The amino acids were detected and identified as aspartic acid, glutamic acid, cystine, serine, glycine, lysine, threonine, histidine, alanine, arginine, valine, methionine, tyrosine, phenylalanine, leucine, iso-leucine and tryptophan in the juice of salted-oyster by using the method of paper chromatography, and the all essential amino acids from nutritional view point was admitted in this juice. 3) The juice contained 0.264mg/ml of tyrosine, 0.304mg/ml of arginine, 0.046mg/ml of tryptophan, 0.460mg/ml of histidine, 0.350mg/ml of methionine, 0.440mg/ml of cystine and 0.115mg/ml of phenylalanine.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.470-470
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• 2012

우수관거 시스템에서의 신뢰도 분석에 대한 기존 연구들은 시스템의 설계상에서의 각종 매개변수들에 대한 불확실성 분석에 기초하여 왔다. 그러나 본 연구에서는 동일한 설계빈도하에 설계된 우수관망이라 하더라도 관망의 노선 선정에 따라서 유출구에서의 첨두유출량 및 초과강우사상에 따른 침수 발생량이 달라질 수 있다는 점에 주목하였다. 그러므로 신뢰도 높은 우수관망을 설계하기 위해서는 이러한 침수 발생 확률을 줄일 수 있도록 하여야 하며, 이를 위해서는 관망 구성에 따라 달라지는 신뢰도를 하나의 정량화된 수치로 나타야 한다. 본 연구에서는 설계빈도를 초과하는 강우사상들에 대하여 해당 우수관망의 월류 발생 정도를 정량적으로 평가함으로써 상대적인 신뢰도를 하나의 지표로서 나타내고자 하였다. 이때 고려되는 것은 초과강우사상 발생 시 해당 관망에서의 월류 발생량 및 월류 발생 지점 개수이다. 또한 이때 고려 대상이 되는 월류량 및 월류 발생지점 수는 서로 다른 척도를 갖는 항목이므로 이에 대한 종합적인 고려를 위하여 본 연구에서는 다기준의사결정기법 중 하나인 DMM(Distance Measure Method)을 이용하였다. 본 연구에서 개발한 우수관망 신뢰도의 산정 절차는 다음과 같다. step 1) 초과빈도별 월류 발생량 및 월류 발생 지점 수 산정 step 2) 빈도별 월류발생량 비율( ) 및 월류발생지점 비율($N_i$) 산정 $$V_i$$ $$V_o/V/R$$ $$N_i=N_o/N_T/R$$ 여기서, $V_i$는 적용된 강우량당 유역의 전체 유출량 대비 월류발생량을 나타내며, $N_i$는 적용된 강우량당 해당 관망의 전체 지점 수 대비 월류 발생지점 수를 나타낸다. step 3) 중심점(central point)에 대하여 DMM을 이용한 치수안전성 산정 $$Reliability\;of\;Sewer\;Networks=1-\;{(1-N_i)^2+(1-V_i)^2\atop2}$$.

• Korean Chemical Engineering Research
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• v.44 no.1
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• pp.35-39
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• 2006

This study was performed to understand temperature effect on retention behavior of fructose and glucose as sugars and lactic acid and acetic acid as organic acids on poly (4-vinylpyridine) resin. The pulse tests were performed to understand temperature effect on retention time of sugars and the results were not shown large change. As it was able to predict with PVP resin not to be used for sugar separation generally, the results were shown poor resolution for separation of sugars and temperature effect on the resolution change of sugars also was not large. On the other hand, in the case of organic acids on PVP resin, the pulse tests were shown temperature effect on the retention behavior was very large. So, the frontal analyses were performed to understand quantitative adsorption behavior of organic acids at 35 and $65^{\circ}C$. These adsorption characteristics of organic acids with PVP resin system can be used to preparative chromatographic process such as SMB (simulated moving bed).

• Korean Journal of Food Science and Technology
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• v.19 no.6
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• pp.528-532
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• 1987

Lipids of kidney bean were extracted by the mixture of chloroform-methanol-water (1:2:0.8 v/v), fractionated into neutral lipids, glycolipids and phospholipids by silicic acid column chromatography, and the composition of these lipid classes were determined by TLC and GLC. The lipid content of kidney bean was 1.9%, and the lipid was consisted of 48.3% neutral lipids. 7.5% glycolipids and 44.2% phospholipids. Triglyceride was the major component of neutral lipids (64.6%). The major glycolipid and phosphlolipid were esterified steryl glycoside (38.3%) and phosphatidyl choline (32.9%). The major fatty acids of kidney bean lipid were linolenic, linoleic, palmitic and oleic acid. Linolenic acid contents were very high to be 37.1% in total lipid and 50.5% in neutral lipid.

• Food Science of Animal Resources
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• v.29 no.4
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• pp.487-493
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• 2009

This study was conducted to investigate the effect of various combinations of sugars, amino acids, and fatty acids (oleic/linoleic aicds) on volatile compounds of low-fat sausages (LFSs) to have similar characteristics to those with regular-fat counterparts (RFC). The addition of glucose or fructose alone in LFC increased the concentrations of myristicine, pentadecanal and octadecanal, as compared to RFC. In addition, LFSs containing lysine alone had higher concentration of myristicine, as compared to those with RFC. Without the addition of both oleic and linoleic acids, LFS containing glucose in combination with various amino acids, such as alanine, aspartic acid, cysteine, and lysine, had higher concentration of myristicine, as compared to RFC. Furthermore, myristicine content (ppm) of LFSs was different from those of RFC, regardless of the concentration of lysine in combined with glucose or fructose. LFSs containing oleic and linoleic acids with the combination of glucose/glycine or glucose/alanine had the most similar volatile compounds to those of RFC. These results indicated that volatile compounds isolated from smoked LFSs containing sodium caseinate as a fat replacer and the multiple combinations of sugar, amino acids, and fatty acids had the most similar volatile compounds to those of RFC.