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http://dx.doi.org/10.5010/JPB.2006.33.4.283

Metabolic Discrimination of Rice Cultivars and Relative Quantification of Major Sugar Compounds Using 1H NMR Spectroscopy Combined by Multivariate Statistical Analysis  

Kim, Suk-Weon (Biological Resources Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Koo, Bon-Cho (Biological Resources Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Kim, Jong-Hyun (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Liu, Jang-Ryol (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Publication Information
Journal of Plant Biotechnology / v.33, no.4, 2006 , pp. 283-288 More about this Journal
Abstract
Discrimination of 5 rice cultivars (Sangjubyeo , Dongjinbyeo Simbaekbyeo , Hwamanbyeo , and Simbaek-hetero ) using metabolic profiling was carried out. Whole cell extracts from each cultivar were subjected to $^1H$ NMR spectroscopy. When spectral data were analyzed by principal component analysis, 5 cultivars were clustered into 3 groups: SJ, DJ + SB, and HM + SH. Thecultivars showed great difference in carbohydrate region of $^1H$ NMR spectra, suggesting that qualitative and quantitative differences in carbohydrate compounds play a major role in discrimination of the cultivars. In addition, it was readily possible to determine relative quantification of major carbohydrates including sucrose, glucose, maltose from spectral data of the cultivars. SJ showed 2 to 4 times higher content of maltose than the other rice cultivars. Overall results indicate that metabolic discrimination of rice cultivars using $^1H$ NMR spectroscopy combined by multivariate statistical analysis can be used for rapid discrimination of numerous rice cultivars and simple quantitative analysis system of major carbohydrate compounds in rice grains.
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