• Analytical Science and Technology
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• v.16 no.6
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• pp.475-482
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• 2003

Uncertainty of final measurement results considering main uncertainty sources being in dioxin analysis of a blood sample was estimated. 'The Guide to the Expression of Uncertainty in Measurement' was suggested for accomplishment of this study. After uncertainties for the 11 compounds detected in this experiment of the 17 target compounds of dioxin and furan were calculated considering the uncertainty sources of each step, uncertainty for the total dioxin concentration was estimated by combining these values. The concentration of dioxin in blood sample was expressed as $0.0746{\pm}0.0074pg$ I-TEQ/g weight or $20.68{\pm}2.04pg$ I-TEQ/g lipid, including the uncertainty values obtained in this way. The former expression indicates the conversion concentration into the sample weight and the latter one indicates the conversion concentration into the lipid weight. The quality of measured analytical results could be assured quantitatively by estimating uncertainty of measurement results and showing the range of measurand.

• Korean Journal of Food Science and Technology
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• v.16 no.1
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• pp.51-58
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• 1984

Grains of naked barley (Baekdong cultivar) were polished, powdered and subjected to the successive extraction into free and bound liquid fractions. These were further fractionated into lipid classes and quantified by means of thin layer chromatography, column chromatography and gas-liquid chromatography. Contents of free and bound lipids in barley flour were 2.27% and 1.01%, which were decreased to 2.12% and 0.76%, respectively, after purification. Free and bound lipids were consisted of monoglycerides, diglycerides, triglycerides, free sterols, sterol esters, free fatty acids and polar lipids. Major constituents of free lipids were 56.2% triglycerides, 14.9% free fatty acids and 13.4% sterols while those of bound lipids were 73.8% polar lipids, 8.4% free fatty acids and 5.2% triglycerides. The content of non-polar lipids in free lipids was 93.6% as compared with 26.2% in bound lipids. However, phospholipids content in bound lipids was 55.5% as compared with 2.5% in free lipids, and glycolipids content in bound lipids was 19.4% as compared with 3.9% in free lipids. Major fatty acids in the free and bound lipid fractions were linoleic acid 52.1%, 54.8%, palmitic acid 24.8%, 30.0% and oleic acid 15.6%, 8.8%, respectively, showing similar patterns in both fractions. The amount of unsaturated fatty acids in free lipids was 72.8% as compared with 68.0% in bound lipids. In comparing the fatty acid composition of non-polar lipids, glycolipids and phospholipids, no difference was observed between free and bound lipid fractions while a slight difference was found among the lipid constituents.

• Korean Journal of Food Science and Technology
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• v.3 no.3
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• pp.168-171
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• 1971

Estimable taste constituents of the honey, e.g. amino acids, organic acids and sugars, were studied to evaluate the quality as well as the quantity of the honey obtained from several different origin. In the level of sugars and its components were very likely at all kinds of honey but sucrose amounts were slightly higher in feeding honey. Amino acids were highest in wild honey not only in its amount but its variety. Main components of the amino acids were found out as proline in feeding honey and in Tillaceae tree honey and as isoeucine in wild honey. Feeding honey demonstrates highest level of organic acids and the major constituents were oxalic acid and citric acid from wild honey, lactic acid from Tillaceae tree honey, and succinic acid from the feeding honey.

• Food Science and Preservation
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• v.15 no.4
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• pp.524-531
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• 2008

To investigate tea prepared from Ixeris dentata root, color, and levels of free sugars, organic acids, cynaroside, total polyphenolics, and free amino acids were investigated using various thermal processing methods. These included natural drying(ND), hot-air drying(HAD, $75^{\circ}C$), hot-air drying after steam(HADS, steaming at $95^{\circ}C$ for 30 min) and roasting after hot-air drying(RHAD, roasting at $140^{\circ}C$ for $2{\sim}3$ min). Total free sugars, organic acids, cynaroside, and free amino acids contents were highest after ND treatment. Total polyphenolics was highest after RHAD processing. The level of cynaroside, a principal component of Ixeris dentata root, was significantly decreased by HADS. The major free sugars were fructose, glucose and sucrose. Succinic acid was prominent among organic acids, and was present at 2.25%(v/v) after ND treatment. The lightness(L), redness(a), and yellowness(b) of all dried Ixeris dentata root powders were best after RHAD treatment, but analysis of the leaching liquids after hot water treatment yielded variable results. Lightness and redness were the highest after RHAD and yellowness was the highest after ND treatment. Overall, the RHAD drying method was found to be superior to other methods, in sensory evaluation tests.

• Journal of the Korean Wood Science and Technology
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• v.27 no.3
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• pp.91-98
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• 1999

The content of taxol and related compounds in needle and stem tissues of exotic yews growing in the Chollipo Arboretum which located in the West Sea of Korea were determined by HPLC. A considerable variation in contents of taxol and the related compounds within species and among cultivars were observed. The content of taxol was much higher in the needles than those in the young stem tissues except T. X media cv. Vermuleun, T. baccata cv. Washingtonii, and T. X cuspidata cv. Nana, and the content of 10-deacetyl baccatin III(10-DAB III) was also slightly higher in the needle. The needle of T. cuspidata cv. Nigra collected from April was contained the high amount of taxol(1,027 ${\mu}g$ per g dry weight) and also contained high quantity in 10-DAB III(267 ${\mu}g$ per g dry weight). Concentration of taxol in needle was significantly greater than those of meristemic and ovary tissues, Exotic yews growing at the Chollipo Arboretum containing T. cuspidata cv. Nigra could be suitable materials as a source for the tissue and cell cultures for production of taxol.

• Korea Trade Review
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• v.42 no.4
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• pp.93-114
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• 2017

Under the FTA agreement, preferential tariffs between FTA members will result in tariff reductions. In order to ensure the stable use of the FTA tariff system, it is necessary for the customs authorities to determine whether the origin goods are clearly applicable. This study analyzed the procedure of appeal according to the origin verification system based on the decision made by Korea Customs Service and Tax Tribunal. From this, we examined whether the rate of re-claiming a case rejected in the 'Review System of the Legality Before Taxation' differs. In addition, we carried out a quantitative analysis using bootstrapping technique in order to overcome the scarcity cases of verification of origin among FTA members. The implications of this paper are summarized as follows: First, we tested the hypothesis that the re-claiming rate of Western countries is higher. Second, some issues represented higher re-claiming rate. Third, there was no significant difference between the verification group and the re-claiming rate. Finally, even if an applicant makes a claim again, there is a possibility of being rejected again.

• The Korean Journal of Ecology
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• v.23 no.3
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• pp.281-291
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• 2000

The objectives of this study were to classify quantitatively vegetational types of P. densiflora for. erecta forest. The results were summarized as follows. 1) According to the canonical correspondence analysis(CCA), variables such as topography, altitude, available P/sub 2/O/sub 5/, and Mg/sup 2＋/were highly correlated on three axes. Based on the correlations between vegetation units and environmental factors, Rhododendron micranthum community tended to be more distributed in the such situations of high altitude, upper topography, high C/N ratio, low level of Mg/sup 2＋/and Ca/sup 2＋/. Vaccinium koreanum community tended to be more distributed in the such conditions of high altitude, upper topography, high level of Ca/sup 2＋/and Ma/sup 2＋/. 2) According to the results of importance value analysis, Tree layer and Subtree layer in study areas showed high Ⅳ along with P. densiflora for. erecta, Quercus mongonlica, Q. variabilis, Q. serrata, Fraxinus rhychophylla and shrub layer indicated that Ⅳ of P. densiflora for, erecta in Sokwang-ri area was higher than those of the other areas. Therefore, it was considered that P. densiflora for. erecta forest of Sokwang-ri area could be naturally regenerated. 3) According to results of species diversity, the species diversity of Uljin region showed the least low value as 0.6622, other regions appeared about 0.7. In the diversity by vegetation types, Acer pseudosieboldianum community showed high value. 4) According to the analysis of community similarity, Sokwang-Ri region was lowly correlated with Uljin region, while the index among the other areas appeared above 0.6. 5) According to the analysis of interspecific association, it was divided into 2 groups. The species of first group were mainly involved in character species, and the species of second group were involved in differential species groups.

• Journal of Radiation Protection and Research
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• v.22 no.2
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• pp.103-109
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• 1997

Adult male rats were exposed to a whole body with a single dose of 1.0, 2.0, 3.0, 5.0, and 7.0 Gy. The animals were sacrificed 48, 72, 96 and 216 hours following exposure. A competitive enzyme linked immunosorbent assay(ELISA) with antigen immobilized on the solid phase has been developed to measure ceruloplasmin in rat serum and complete dose response curves. Ceruloplasmin was purified from the plasma of turpentine treated male rats. Coating of ceruloplasmin had more effectiveness in 10 mM Tris-HCI, 150 mM sodium chloride, pH 7.4 than in 50 mM carbonate/bicarbonate buffer, pH 9.6. The coating range for ceruloplasmin was $70{\sim}140ng$/well. Levels of ceruloplasmin increased to maximum on the $72{\sim}96$ hours after irradiation. Slope of between response and dose was greatest value 96 hours following irradiation. Normal ceruloplasmin levels were not recorded 216 hours following exposure. In 0.1 Gy irradiated group, levels of ceruloplasmin also increased to maximum on the $72{\sim}96$ hours following irradiation. The concentration of this protein remained significantly different from control value, 196 hours after exposure. Ceruloplasmin was identified as one of the major acute phase protein following irradiation and further studies about gene expression and regulation would be necessary for radiation protection.

• Applied Microscopy
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• v.36 no.2
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• pp.83-91
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• 2006

The number and structure of synapses are dynamically changed in response to diverse physiological and pathological conditions. Since strength of synaptic transmission is closely related to the synaptic density on a neuron, both synaptogenesis and synapse loss may play important roles in controlling neuronal activity. Thus it is essential to estimate the number of synapses using an accurate quantitative method for better understanding of the numerical alteration of synapses under terrain experimental conditions. We applied physical disector principle to estimating the number of synapses per neuron in the dentate gyrus of adult mice. First, we measured the numerical density of granule cells using the physical disector principle. Second, the density of medial perforant path to granule cell synapses was estimated using the bidirectional physical disector. Then, the volume ratio of molecular layer to granule cell layer was measured. With these numerial values, we successfully calculated the number of synapses per neuron. Individual granule cells have approximately 6500 synapses in the dentate gyrus of adult mice $(6,545{\pm}330)$, which are comparable to those of other researchers. Our results showed that the estimation of synapse numbers per neuron using the physical disector principle would provide accurate and precise information on the numerical alteration of synapses in diverse physiological and pathological conditions. Following analyses of synapse numbers using this method will contribute to the better understanding of structural synaptic plasticity in a variety of experimental animal models.

• Journal of Biosystems Engineering
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• v.21 no.4
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• pp.414-421
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• 1996

한국과 일본의 경우 건표고를 외관의 품질상태 에 따라 12등급에서 16등급으로 구분하고 있다. 그리고 등급판정 작업은 임의로 추출한 샘플을 대상으로 전문 감정가에 의해 수작업으로 수행되고 있다. 건표고의 품질을 결정짓는 외관의 품질인자들은 갓과 내피에 고루 분포하고 있다. 본 논문에서는 컴퓨터 영상처리 시스템에 의거하여 개발한 건표고 자동 등급판정 및 선별 시작시스템의 구조와 기능 그리고 성능에 대하여 설명하였다. 개발한 시작시스템은 표고의 이송과 취급자동화를 위한 진동이송기, 반전장치, 컨베이어 이송장치와 두 세트의 컴퓨터 영상처리 시스템, 그리고 시스템 통괄제어를 위한 IBM PC AT호환 컴퓨터, 디지털 입출력 보드, 전공압실린더 구동제어를 위한 PLC등으로 구성하였다. 등급판정의 효율성 및 실시간 작업시스템을 고려하여 건표고의 등급판정은 두 세트의 컴퓨터 영상처리 시스템을 이용하여 이송되는 건표고의 갓 또는 내피 중 어디가 위를 향하는 지에 따라 두 단계에 걸쳐 독립적으로 판정을 수행하도록 하였다. 첫 번째 영상처리부에서는 갓표면 영상으로부터 4등급의 고품질 표고를 분류하며 두 번째 영상처리부에서는 내피표면 영상으로부터 중간 및 저품질 표고를 8개의 등급으로 분류한다. 실시간 영상정보처리를 목적으로 기존에 개발한 신경회로망을 이용한 등급판정 알고리즘을 시작시스템에 적용하였다. 개발한 시작기는 88% 이상의 등급판정 정확도를 보여 주었으며, 전공압시스템의 구동제약으로 인하여 표고 1개당 약0.7초의 선별시간이 소요되었다. 일조 선별라인의 경우 본 연구에서 제안한 시작기의 선별능력은 표고가 일차 처리부로 갓이 위로 올라와 있는 상태로 계속 공급된다면 시간당 대략 5,000여 개의 표고를 처리할 수 있을 것으로 기대된다.보강하여 가능하면 B-Pillar의 Middle이 Bending type collapse을 방지하여 Pelvis와 Door가 먼저 접촉하는 방법 등이 적용가능하다. 제작하기 이전에 설계된 부품에 대한 스프링 상수 및 내구특성을 체계적으로 규명하여 제품 시험의 횟수를 줄이고, 보다 정밀한 제품을 제작할 수 있도록 하기 위한 것이다.세포수는 초기 배반포기배에서 팽윤 배반포기배로 진행됨에 따라 두배에서 세배 정도 증가되었음을 알 수 있었다. 또한, differential labelling과 bisbenzimide기법에서 얻어진 각각의 총세포수를 비교하였을 때 총세포수는 발달의 진행 정도에 따라 증가되며 그와 동시에 동일한 군 간의 세포수도 거의 유사함을 알 수 있었다. 따라서, ICM과 TE를 differential labelling하는 기법은 수정란의 quality를 평가하는데 매우 유용한 기법으로서 착상전 embryo 발달을 연구하는데 효과적으로 이용될 수 있다는 것을 시사한다. 고도의 유의차를 나타낸 반면 비수구, 초생수로구 및 Bromegrass 목초구 간에는 아무런 유의차가 인정되지 않았다. 7. 농지보전 처리구인 배수구와 초생수로구는 비처리구에 비해 낮은 침두 유출량과 낮은 토양유실량을 나타내었다.구보다 14% 절감되는 것으로 나타났다.작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간을 연장시키는 경향을 보이며 큰