• Childhood Kidney Diseases
• /
• v.12 no.2
• /
• pp.150-156
• /
• 2008

Purpose : Many results have reported a correlation between the spot urine protein/creatinine ratio(P/C ratio) and 24-hour urinary protein(24UP) amount. This study was designed to evaluated correlation between 24UP amounts and P/C ratio in children and to find the factors that affect this correlation. Methods : 210 patients who visited the Department of Pediatrics in Busan Paik Hospital from september 2003 to december 2007 were included in this study. All the patients were divided into I, II, III/A, B, C group[I:24UP(mg/$m^2$/day)]<100, II: 100$\leq$24UP<1,000, III: 24UP$\geq$1,000, A: Cr excretion(mg/kg)<15, B: 15$\leq$Cr excretion<25, C: Cr excretion$\geq$25)]. Pearson correlation analysis was performed between 24UP and P/C ratio to evaluate the relationship. We defined fractional difference between 24UP and P/C ratio, and then performed multiple regression analysis. Results : There was a strong positive linear correlation between 24UP and P/C ratio in all patients, and the correlation was also good in each group. The factors affecting accurate quantitation of proteinuria using spot urine P/C ratio was creatinine excretion. Conclusion : Spot urine P/C ratio is a useful test to predict proteinuria roughly. Therefore, we expect that urine P/C ratio can be used as parameter instead of 24UP, if we set cutoff value of P/C ratio considered to creatinine excretion according to age and sex in large pediatric population.

• Proceedings of the Korea Society of Environmental Toocicology Conference
• /
• 2004.05a
• /
• pp.26-26
• /
• 2004

• Proceedings of the Korean Information Science Society Conference
• /
• 2005.11b
• /
• pp.238-240
• /
• 2005

단백체는 세포가 처해있는 환경에 따라, 그리고 각 조직 별로 유동적으로 존재하며, 세포의 실제적인 기능을 표현해준다. 이러한 이유로 세포 내에서 일어나는 실제적인 현상들을 전체 단백질 단계에서 통합적으로 파악하고자 하는 단백체학 연구가 활발하게 진행되고 있다. 미지의 단백질의 기능을 밝혀내는 연구는 단백체학의 가장 기본적이면서 중요한 부분이라고 할 수 있다. 본 논문에서는 "단백질 상호작용 네트웍 사전(PIND)"을 구축함으로써 단백질의 기능을 예측하는 새로운 방법론을 소개한다.

• The Korean Journal of Mycology
• /
• v.19 no.1
• /
• pp.79-84
• /
• 1991

Fruit bodies of Ganoderma lucidum have been used for most pharmacological studies, but pharmacological effects are likely variable because the habitats and strains of Ganoderma lucidum are different. Therefore, their fermentation is required to produce constant and reliable pharmacolo­gical constituents from Ganoderma lucidum. During the studies of medium for industrial application. it was found that ginseng root residues, remaining after being extracted with ethanol, were a good carbon source for a fermentation of Genoderma lucidum and a corn steep liquor was also economical for the nitrogen source. Yield of the mycelial cultured in ginseng root residues and corn steep liquor was 2.5 times higher than that in glucose and peptone, known as a conventional medium of Ganoderma lucidum. The polysaccharide content of the extracts from the cultured mycelia was higher than that from fruit bodies, but protein content was vice versa. Extracts of the cultured mycelia were more effective and lasting than extracts of the fruit bodies in decreased hypertention of spontaneously hypertensive rats (SHR).

• Proceedings of the Korea Contents Association Conference
• /
• 2009.05a
• /
• pp.306-311
• /
• 2009

We initially obtained human diseases-related proteins dataset from the OMIM and the SWISS PROT and then constructed disease-related protein-protein interaction network. The protein network contains 40 hub proteins such as CALM1, ACTB and ABL2. The protein network can be derived the map of the relationship between different disease proteins, denoted disease interaction network. We demonstrate that the associations between diseases are directly correlated to their underlying protein-protein interaction networks. From constructed the disease-protein bipartite network, we derived 38 diseasomal proteins, including APP, ABL1 and STAT1. We previously demonstrated that hub proteins in the network tend to be diseasomal proteins in the disease-related protein sub-networks. However, we found that 18% hubs are only diseasomal proteins in the whole disease network. At this point, we could not elucidate difference in the hub-diseasomal proteins tendency between sub0network and whole network. In spite of we still have unsolved problems, our results elucidate that the discovery of protein interaction networks assigned by diseases will provide insight into the underlying molecular mechanisms and biological processes in complex human disease system.

• The Korean Journal of Food And Nutrition
• /
• v.17 no.2
• /
• pp.177-185
• /
• 2004

The effects of liquid culture conditions and nutrient sources on the formation of protein-binding polysaccharide (PS) in Cordyceps militaris were examined. The formation amount of PS was increased in proportion to the growth rate of mycelium, in case of higher aeration or lower acidity. The optimum growth temperature of the mycelia was 25$^{\circ}C$ for the formation of PS. The optimum carbon source and nitrogen source were glucose and peptone, respectively. The ratio of C/N was optimal with 3％ glucose to 0.5 ％ peptone. The sugar composition in the PS was greatly changed according to the carbon sources. The mycelium of Cordyceps militaris by liquid culture showed a higher electron donating ability than that by solid culture.

• Proceedings of the Korea Contents Association Conference
• /
• 2016.05a
• /
• pp.193-194
• /
• 2016

세포사멸(apoptosis)이란 유전자에 의해 제어되는 세포의 능동적인 죽음을 의미하며 진핵세포만이 가지는 기작으로 세포자살을 일컫는 말이다. 단백질 정보 DB인 UNIPROT으로부터 진핵 생물 종의 세포사멸 단백질(apoptotic protein)을 수집하여 아미노산 서열에 대한 서열비교분석(alignment)을 진행하였다. 그 결과에 따라 다양한 종에 걸쳐 그 서열이 유사하게 유지되는 세포사멸 단백질을 중심단백질로 선정하였다. 비교단백체 분석을 통해서 생물 계통도에서 보여지는 생명종과 이들 단백질과의 연계성을 비교 분석함으로써 단순한 진핵세포에서 점진적으로 확대되는 종까지의 세포사멸 과정을 추론하였다.

• Applied Microscopy
• /
• v.29 no.4
• /
• pp.499-509
• /
• 1999

Accumulations of the storage proteins in protein storage vacuole and the differentiation of protein bodies from protein-filled ER in developing pea cotyledons have been investigated using conventional and immunoelectron microscopy. To improve the fixation quality, single cells separated enzymatically from sliced cotyledons were used. At early stages of seed development osmiophilic protein accumulates in rER lumen were observed quite often. This protein-filled ER cisternae were differentiated into cytoplasmic protein bodies at late stage by the process called terminal dilations which have been considered a principal route of the formation of cytoplasmic protein bodies somewhat later in seed maturation. Immunocytochemical labellings of the vicilin and legumin show that presence of vicilin on both of the cytoplasmic PB and PD, but limited presence of legumin only on the cytoplasmic PB at intermediate stage of seed development. Immunogold labellings of Bip, ER retention protein, were observed on the inner periphery of protein deposits in protein storage vacuole. This result was regarded that Bip can recognize and retrieve misfolded protein during active accumulation of storage protein to the PD in PSV.

• Bulletin of Food Technology
• /
• v.18 no.1
• /
• pp.3-15
• /
• 2005

최근 들어 게놈기능연구는 주요국가의 새로운 국가적 연구표적으로 지정되면서 유전자기반 생물산업의 핵심으로 부각되고 있다. 이러한 발전은인간(2001년 2월 인간 게놈의 초안 발표)을 비롯한 생물체의 게놈구조가 규명되어 이 유전자구조정보를 web상에서 쉽게 알아낼 수 있는데서 비롯된다. 포스트게놈시대의 게놈기능연구를 총괄적으로 '기능유전체학 (Functional Genomics)'이라고하며 여기에는 핵산(DNA나 RNA)을 표적으로 게놈기능을 연구하는 genomics(유전체학, RNA발현을 대상으로 하는 transcriptomics(전사체학) 포함), 총체적인 단백체를 대상으로 유전자기능을 연구하는 proteomics(단백질체학) 및 대사물질을 대상으로 하는 metabolomics(대사체학), 이들 분야를 공통적으로 지원하는 bioinformatics(생물정보학)로 구분된다. 본 고에서는 프로테오믹스 분야를 중심으로 소개하고자 한다.

• Food Science and Industry
• /
• v.51 no.4
• /
• pp.270-277
• /
• 2018

The rising global demand for food and beverages with higher protein content provides manufacturers with great opportunities for innovation and premium positioning of their products as healthy choices. However, the market price volatility and supply risks associated with animal-based proteins can quickly erode margins and profitability. A diversified protein strategy that includes plant-based soy protein greatly improves your ability to predict profitability over time, while maintaining or even improving product quality.