• Korean Journal of Poultry Science
• /
• v.36 no.2
• /
• pp.139-147
• /
• 2009

This experiment was conducted to investigate the effect of organic dietary ME and CP on performance, blood composition and meat quality of cross-bred chicks for 15 weeks. Experiments were factorially designed with 3,000, 3,050, and 3,100 kcal of ME/kg, and 21 and 22% CP for the first five weeks; 3,050, 3,100, and 3,150 kcal of ME/kg, and 19 and 20% CP for the second five weeks; and 3,100 and 3,150 kcal of ME/kg, and 17 and 18% CP for the rest of weeks. Each treatment consisted of four replicates. Weight gain increased in treatments with higher ME diets for the first five weeks, but feed intake decreased significantly as dietary ME increased (P<0.05). Feed conversion also improved in chicks fed 3,100 kcal of ME/kg diets compared with those of 3,000, and 3,050 kcal of ME/kg (P<0.05), but was not influenced by dietary protein. Weight gain and feed conversion showed similar tendency for the second five weeks to those of the first five weeks. However, feed conversion improved significantly in 3,100 and 3,150 kcal of ME /kg compared to 3,050 kcal of ME/kg (P<0.05). Dietary protein did not affect the performance at all for this period. Weight gain and feed intake tended to increase in higher ME and lower protein diets from 11 to 15 weeks of age, whereas feed conversion decreased in 3,150 kcal of ME/kg more than 3,000 kcal/kg. No difference was found by CP treatments (P<0.05). There were no interactions in performance, blood composition and meat quality between dietary ME and protein. Further studies need to describe in whole period experiments instead of periodical experiment.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.37 no.6
• /
• pp.721-728
• /
• 2008

Hepcidin is a peptide hormone produced by the liver, of which secretion is closely related to iron status in the body. However, little is known about the molecular mechanism(s) by which this peptide regulates body iron homeostasis. The purpose of this study was to determine the effects of hepcidin treatment within the physiological concentration range on the expressions of two different iron transporter proteins-ferroportin (FPN) and divalent metal transporter 1 (DMT1). Differentiated Caco-2 intestinal cells and macrophage J774 cells were treated with either synthetic hepcidin or hepcidin-rich fraction separated from human urine at the concentration of 10 nM and 100 nM for 24 hours. Results show that hepcidin treatment in differentiated Caco-2 cells or in J774 cells did not change the level of either FPN mRNA or DMT1 mRNA. On the other hand, hepcidin treatment at the dose of 100 nM significantly decreased the FPN protein levels and DMT1 protein levels in differentiated Caco-2 cells. Similarly, urinary hepcidin treatment (10 nM & 100 nM) also significantly decreased the levels of FPN and DMT1 proteins in J774 macrophage cells. These results showed that hepcidin might play an important role in the regulation of iron homeostasis by lowering the protein levels of iron transporter FPN and DMT1 both in enterocytes and in macrophage cells.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.4
• /
• pp.442-450
• /
• 2009

Piperine is an alkaloid-amine found in pepper and has been reported to have anticarcinogenic properties. To explore the possibility that piperine has cancer chemopreventive and chemotherapeutic effects in colon cancer, we examined whether piperine inhibits the growth of HT-29 human colon cancer cells and investigated the mechanisms for this effect. Cells were cultured with various concentrations ($0{\sim}40{\mu}M$) of piperine. Piperine decreased the cell viability and induced apoptosis of HT-29 cells. Western blot analysis of total cell lysates revealed that piperine decreases the protein levels of Bcl-2, Mcl-1, and intact Bid but increases Bik levels. Piperine increased the percentage of cells with depolarized mitochondrial membrane, and the release of cytochrome c into cytoplasm. Piperine induced the cleavage of poly (ADP-ribose) polymerase and caspases 8, 9, 7, and 3 and increased the Fas levels. In addition, piperine significantly decreased the protein levels of survivin. The present results indicate that piperine inhibits the growth of HT-29 colon cancer cells by the induction of apoptosis, which may be mediated by its ability to change the Bcl-2 family proteins, increase the activation of caspases, and decrease survivin levels. Overall, our findings suggest that piperine has cancer chemotherapeutic effects in colon cancer.

• Proceedings of the Korean Society of Fisheries Technology Conference
• /
• 2000.05a
• /
• pp.322-323
• /
• 2000

점농어, Lateolabrax maculatus는 옛부터 우리나라의 횟감으로 이용되던 어종으로 대 크기가 100cm를 넘는 대형종이다. 그리고, 성장이 빠르고 담수적응력이 강할 뿐만 아니라 수온변화에도 강해 해상가두리 및 축제식 양식대상어종으로 선호하고 있는 실정이다. 이러한 점농어의 양식활성화를 위해서는 전용 배합사료의 개발이 시급하다. 그러나 배합사료개발에 관한 연구는 거의 없어 효율적인 해면양식이 이루어지고 있지 않다. 본 연구에서는 점농어의 생산량 증대를 위한 일련의 연구로 점농어 배합사료 개발에 필요한 기초자료를 제공하기 위하여 단백질 수준을 달리한 사료를 제작, 공급하여 점농어의 사료내 단백질 요구량 수준을 알아보고자 실시하였다. (중략)

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.20 no.4
• /
• pp.337-345
• /
• 1991

This study was designed to investigate the effects of dietary protein ana calcium levels on hematological properties and renal functions of the Pb-administered rats. Male Sprague-Dawley rats were assigned to a 3$\times$3$\times$2 factorial design with 3 levels of protein (40％, 16％, 6％), 3 levels of Ca (1.2％, 0.6％, 0.12％) and 2 feeding Periods (3 and 7 Weeks). The Control group was included separately, The rats were exposed to the drinking water containing 2, 000ppm of lead. Hematocrit, hemoglobin content and RBC count were lower in the Pb-added groups than in the control group and were reduced with decreasing dietary protein and Ca levels. Urinary-aminolevulinic acid was higher in the Pb-added groups than in the control group and increased with decreasing dietary Ca level. However, urinary glucose was higher in the Pb-added groups than in the control group and enhanced with decreasing dietary protein and Ca levels. Creatinine celarance was not affected by the Pb administration when the rats fed the diet containing sufficient protein and Ca.

• Journal of Nutrition and Health
• /
• v.42 no.7
• /
• pp.605-614
• /
• 2009

Most cancer patients are treated with surgery, chemotherapy or radiation as anticancer therapies. Especially in the case of radiation, these treatments produce adverse effects such as vomiting, weight loss, anorexia, normal cell damage and malabsorption. The major goal of this study was to determine the effect of irradiation on the nutritional and immune status in irradiated rats. A secondary goal was to determine the effectiveness of high protein diet (HP) and resveratrol (Res) in minimizing the adverse effects of radiation. Rats were divided into four groups: normal diet (NP), HP, NP + Res and HP + Res groups. Each group was further divided into subgroups that received radiation (RT group) and one that did not (non-RT group). Each diet was supplied from $12^{th}$ day prior to irradiation treatment with irradiation dose of 17.5 Gy. The diets were continued until 10th day after radiation treatment and animals were sacrificed. The radiation treatment showed decreased body weight, serum protein and HDL levels and increased TG and LDL levels in nutritional status. HP, NP + Res and HP + Res groups reduced the level of serum LDL and TG in irradiated rats. NP + Res and HP + Res groups increased reduced albumin level of serum in RT group. In case of immune status, the radiation treat-ment showed decreased WBC, lymphocytes and increased neutrophil and eosinophil levels. The levels of serum IL-2 and IL-6 were significantly increased by radiation, however the cytokine levels decreased in all dietary treatment groups. These results showed that high protein diet and resveratrol supplementation seem to minimize the adverse effects of radiation on lipid nutritional status and inflammation response in the rat model.

• Korean Journal of Community Nutrition
• /
• v.3 no.2
• /
• pp.218-227
• /
• 1998

This study was performed to investigate the effects of dietary protein and calcium levels on iron and zinc balance in eight healthy Korean young women. The 20-day metabolic study consisted of a 2-day adaptation period followed by three 6-day experimental periods. Three experimental diets were the low protein-low calcium(LProLCa ; protein 44g, Ca 422mg), the high protein-low calcium(HProLCa ; 'protein 85g, Ca 365mg) and the high protein-high calcium (HProHCa ; protein 84g, Ca 727mg). Apparent absorption and balance of iron and zinc were significantly higher when subjects were fed high protein-low calcium diet than low protein-low calcium diet. The elevation of dietary calcium significantly depressed the apparent absorption of iron and zinc. The levels of serum iron and zinc were likely to be increased with a high protein diet, but the differences were not significant. There was a strong correlation(r=O.99) between the iron intake and serum iron concentration. Serum zinc concentration was not correlated with hair zinc. Study results revealed that the levels of dietary protein and calcium influence iron and zinc balance in Koreans. This study suggests that dietary recommendations for trace minerals, such as iron and zinc, should be carefully examined. In addition, there is a need to evaluate the bioavailability of milk or beverage products enriched with calcium and iron.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.20 no.5
• /
• pp.433-439
• /
• 1991

This nutritional survey was conducted from September 15 to 21, 1990, in order to investigate the nutritional status of housewives living in Andong city, rural area of Korea. The subjects of this survey were 222 rural housewives living in Andong area. The results were summarized as follows ; The daily food intake was 1133.1g and that of animal foods was 131.5g. The average intake of nutrients was above the RDA. Total calorie intake was 2123.7kcal. Perentages of energy from carbohydrate, protein and fat were 64.4, 18.1 and 17.5 respectively. The predicted percentages of deficiency was 19.8% in calorie, 10.0% in protein, 14.0% in Ca and 25.2% in Fe of total subjects. The correlation between the income level, food expenditure and protein intake was significant.

• KOREAN POULTRY JOURNAL
• /
• s.9
• /
• pp.27-29
• /
• 1970

초생추, 중추, 대추, 산란, 부로일러 전기, 부로일러 후기 등 각 기별에 따르는 사료 카로리와 단백질수준에 의한 사료의 변화와 이때 주의하여야 할 점 등에 대하여 알아 보자.

• The Korea Swine Journal
• /
• v.23 no.9 s.265
• /
• pp.123-126
• /
• 2001

돼지 성별 사료섭취량 및 체조성과 관련된 시험 결과를 보면 자유채식시 사료섭취량은 거세돈이 암퇘지와 비거세돈보다 많으며, 적육 축적률은 낮다. 체중 30$\~$60kg의 비육기에 자유채식과 제한급여구를 비교한 결과 증체율과 지방축적률은 자유채식에서 높았으며, 거세돈의 에너지 섭취 수준을 $8\%$ 제한시 암퇘지를 자유채식시킨 경우와 비슷한 성장률을 보였다. 또한 80$\~$140kg의 체중에서 최대 단백질 축적량은 수퇘지 42.5kg 암퇘지 40.0kg, 거세돈37.5kg이고, 1일 단백질 축적량은 수퇘지 180g, 암퇘지 160g, 거세돈 145g정도로 성별로 차이가 있다. 체지방축적은 에너지 수준과 거세실시 여부, 일당증체량은 에너지 수준에 큰 영향을 받는다.