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A Review on the Insecticidal Activity of Neem Extracts (Azadirachtin) and its Current Status of Practical use in Korea (님추출물 아자디라크틴의 살충활성과 국내 이용현황에 대한 고찰)

  • Kim, Dong-soon
    • Korean journal of applied entomology
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    • v.60 no.4
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    • pp.463-471
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    • 2021
  • As a tropical plant, the neem tree (Azadirachta indica) has been used for a long time for disease and pest control and medical purposes. In this paper, we reviewed for the active ingredient of neem, the mode of action of azadirachtin in terms of insect growth regulation, repellent, feeding inhibition and oviposition against plant pests. And also we reviewed the current status of practical use in Korea. Among 57 products of neem-based eco-friendly organic agricultural materials distributed in Korea, seven products were certified for their efficacy. The average azadirachtin content of the seven products was 0.38%, which was 5.5 times less than the average content of 2.1% distributed worldwide. In the control effect on neem products in Korea, it showed some variation on aphids, but most showed a control effect of more than 90%. The treatment effects of Lycorma delicatula nymphs were 73-77%, and the control effects for thrips were obtained in the range of 50-72% mortality. The mortality effects against bug and moth species were generally low. It is expected that this review would provide important information necessary for the understanding of distributed neem products and the interpretation of experimental data.

STUDIES ON SECONDARY AMINE IN FOODS ( I ) Contents of Dimethylamine in Fish Muscle (식품중의 제2급 amine에 관한 연구( I ) - 어육중의 Dimethylamine의 함량 -)

  • KIM Chang Yang
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.11 no.1
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    • pp.9-12
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    • 1978
  • Contents of dimethylamine (DMA) in fresh fish muscle were analyzed by gaschromatography. DMA contents were in Pseudosciaena manchuria $10.367mg\%$, in Theragra chalcogramma $19.133mg\%$, in Sardinops melanosticta $22.833mg\%$, and in Scomber japonicus $26.300mg,\%$ respectively. Individual amines could not be separated with such a low content.

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Genotoxicity of Environment-friendly Organic Materials of Plant Origin in the Micronucleus Test Using Chinese Hamster Lung Cells (Chinese Hamster Lung Cell의 소핵시험을 이용한 식물추출물 유기농업자재의 유전독성평가)

  • Cho, Hyeon-Jo;Park, Kyung-Hun;Jeong, Mi Hye;Park, Soo Jin;Oh, Jin-Ah;Kim, Won-Il;Cho, Namjun;Ryu, Jae-Gee;Paik, Min-Kyoung
    • Korean Journal of Environmental Agriculture
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    • v.33 no.2
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    • pp.138-143
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    • 2014
  • BACKGROUND: Azadirachta Indica extract(AIE) and Sophorae radix extract(SRE) are widely used as environment-friendly organic materials of plant origin in South Korea. METHODS AND RESULTS: In this study, the in vitro micronucleus(vitMN) tests of two samples of AIE and SRE were conducted to evaluate their genotoxicity using the Chinese hamster lung(CHL) cell. This study was composed of two parts; cytochalasin B(cyto B) test and non-cyto B test. Mitomycin C and colchicine were used as positive controls. As a result, the incidence of micronucleus(MN) in all AIE and SRE treated groups increased in dose-dependent manner, but were less than 2.2% in 1,000 binucleated cells. In addition, there were no significant increases of MN incidence in all AIE and SRE treated groups, compared with the negative control group. CONCLUSION: Therefore, we suggest that AIE samples and SRE samples used in this study may have no genotoxicity in the in vitro micronucleus test using the CHL cells. In our previous study, we reported that AIE and SRE did not cause genotoxicity in Ames test. According to the genotoxicity battery system, we concluded that AIE and SRE used in this study have no genotoxic effects to humans.

Stability of Four Limonoidal Substances of Neem Extract under Controlled Aquatic and Soil Conditions (님나무 추출물의 Limonoid계 살충성분 4종의 환경매체 노출 안정성)

  • Kim, Jin Hyo;Jeong, Du-Yun;Jin, Cho-Long;Kim, Won-Il;Lim, Sung-Jin;Choi, Geun-Hyoung;Park, Byung-Jun
    • The Korean Journal of Pesticide Science
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    • v.18 no.3
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    • pp.156-160
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    • 2014
  • The stabilities of four limonoidal substances including azadirachtin A, azadirachtin B, deacetylsalannin and salannin were investigated both in controlled aquatic and soil conditions. The half-life of the total limonoid for neem extracts and its two commercial biopesticides was estimated 86.6-173 days in water under air, while degradation of the compounds was detected below 10% after eight weeks in deoxygenated water. The half-life in dry soil was estimated 43.3-57.7 days, and there was a similar degradation pattern with in aerobic water condition. In case of wet soil condition, the total bacteria of the soils ranged 6-8 log CFU/g soil for during the experiment, and the half-life of the total limonoid was 6.4-12.3 days. From the result, the fast limonoid degradation in wet soil environment was the result of both chemical oxidation and microbial degradation.

A Study on the Characteristics of the Narrative and Application Methods of the Modern Elements of , a Drama of tvN (tvN 드라마 <백일의 낭군님>의 내러티브 특징과 현대적 요소의 활용 방식 연구)

  • Youm, Won-Hee
    • Journal of Popular Narrative
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    • v.25 no.1
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    • pp.249-280
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    • 2019
  • This research aims at examining the present of fusion historical dramas through a TV soap opera, and discussing the narrative of this genre and external elements to be equipped. tried to break from conventionality of fusion historical dramas by intensifying it. By comprehensively suggesting the history of conflicts repeated in existing dramas, it helped its viewers to concentrate on it easily. In addition, by setting a separate space from the secret strife in a palace while depicting it, it expressed romance between a man and a woman faithfully. Moreover, two individual riddles were given so that the narrative became rich. Furthermore, the social problems of remaining singles and the tyranny of the establishment were dealt with. Novelty was presented with the viewpoint of seeing the past through today's perspective instead of seeing the present through the past; by trying to interpret modern culture in a way of Joseon, such as the figures reflecting the phases of the times and lines using newly-coined words and abbreviations. Therefore, the success of the drama contents lies not in breaking from customs but in thinking about what to change based on conventional characteristics. can be evaluated to be a work that can be an idealistic model of this genre.

Evaluation of Fish Acute Toxicity and Preliminary Risk Assessment of Plant Extracts, Sophora, Neem and Derris (유기농업자재 고삼, 님 및 데리스 추출물의 어류에 대한 독성과 위해성 평가)

  • Park, Kyung-Hun;Oh, Jin-A;Paik, Min-Kyoung;Son, Mi-Yeon;Lim, Joung-Taek;Jin, Jung-hwa;Hong, Soonsung;Cho, Nam-jun;Han, Sang-Gyun;Kim, Byung-Seok
    • The Korean Journal of Pesticide Science
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    • v.19 no.3
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    • pp.255-263
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    • 2015
  • Promoting the organic farming, much of the plant extracts used for controlling pests and fungi have been imported from China, India and Myanmar. But, it is so worrisome that aquatic animals such as muddy loach inhabiting in paddy field and common carps in river exposed to the pests and fungi likely be harmed. This study was conducted in order to evaluate the risks of aquatic animals influenced by the three plant extracts, i.e. Sophora flavescens, Azadirachta indica and Derris elliptica. The toxicities of common carp (Cyprinus Carpio), muddy loach (Misgurnus anguillicaudatus) and PEC (Predicted environmental concentration) exposed to the three plant extracts were estimated by the typical spray volume method. Risks were determined by the toxicity value as 48-hr $LC_{50}$ (Lethal concentration, median) or NOEC (No observed effect concentration) into PEC. 48-hr $LC_{50}$ of Common carp and NOEC by Sophora flavescens extracts was 7.9 and 6.2 mg/L, 26.8 and 21.8 mg/L by Azadirachta indica extracts and 47.0 and < 24.0 mg/L by Derris elliptica extracts, respectively. 48-hr $LC_{50}$ of Muddy loach and NOEC by Sophora flavescens extracts was 16.9 and 10.0 mg/L, 35.6 and 30.0 mg/L by Azadirachta indica extracts, and 73.9 and < 40 mg/L by Derris elliptica extracts, respectively. Therefore, acute toxicities of the three plant extracts for aquatic animals were proved to be very low level. PEC of Sophora flavescens extracts in paddy, drainage and river water was 68.0~3.0, 11.33~0.50 and 3.0~0.0018 mg/L, respectively. TER of Sophora flavescens extracts in the three water was 0.2~5.6, 1.5~33.8 and 2.6~4388.9, respectively. PEC of Azadirachta indica extracts in paddy, drainage and river water was 90.9~1.2, 15.2~0.2 and 4.8~0.00075 mg/L, respectively. TER of Azadirachta indica extracts in the three water was 0.4~29.7, 2.3~178.0 and 4.5~35733.3, respectively. PEC of Derris elliptica extracts in river water was 0.0063 mg/L. TER of Derris elliptica extracts in river water was 5222~15667.

Study of Kidney Toxicity of Azadirachta Indica Extract for Oral Administration in Rats (님추출물의 경구투여에 따른 랫드의 신장독성 연구)

  • Yoon, Hyunjoo;Choe, Miseon;Cho, Hyeon-Jo;Han, Beom Seok;Park, Kyung-Hun;Oh, Jin-Ah;Cho, Namjun;Paik, Min-Kyoung
    • Korean Journal of Environmental Agriculture
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    • v.33 no.2
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    • pp.103-110
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    • 2014
  • BACKGROUND: Azadirachta indica has been widely used as environment-friendly organic materials because of its insecticidal properties. This study was carried out to investigate the acute toxicity and the subacute toxicity of Azadirachta indica extract(AIE) in rats. METHODS AND RESULTS: For the oral acute toxicity test, Sprague-Dawley rats were gavaged with 2.0 g/Kg bw of AIE. The $LD_{50}$ value was greater than 2.0 g/Kg bw for both male and female rats. For the subacute toxicity study, rats were treated with AIE at doses of 0.5, 1.0, 2.0 mg/Kg bw once a day for 4 weeks(n=10 animals per each group). There were no significant changes in body weight, food intake and water consumption observed during the experimental duration. In addition, no difference of relative kidney weight was observed among all treated groups. Serum creatinine level in the AIE 2.0 g/Kg group increased significantly compared with that of control group in male rats, but serum blood urea nitrogen was significantly decreased in a dose-dependent manner (p<0.05). Significant increase of serum cholesterol levels were observed in all AIE groups, compared with the control group, in the female rats (p<0.05). However, histopathological examination of the kidney did not reveal any significant lesions in all groups. CONCLUSION: On the basis of results, it could be concluded that oral administration AIE didn't cause any toxic response in kidney, except the increased serum cholesterol.