• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.3 s.58
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• pp.173-180
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• 2006

Sesame (Sesamum indicum L.), one of the oldest oilseed crops, has been known to posses antioxidative and inflammatory effects. This seed contains lignan compounds such as sesamin, sesamol, sesaminol, sesaminol diglucosides (SDG), and sesaminol triglucosides (STG). Sesamin, a major lignan in sesame, displayed several biological activities including a protective effects against oxidative damage in the skin. In the present study, we investigated the effect of sesamin, sesamol, sesaminol, SDG, and STG, on nitric oxide (NO) induction and inducible nitric oxide synthane (iNOS) and cyclooxygenases-2 (COX-2) expression in lipopolysaccharides (LPS)-treated RAW 264.7 cells. The results showed that sesamol and sesaminol significantly inhibited NO generation but they were also cytotoxicity however, sesamin effectively inhibited NO production ($IC_{50}: 64{\mu}M$) without my cytotoxic effect in LPS-stimulated macrophage RAW 264.7 cells. In further study, it was founded that sesamin inhibited the expression of inducible nitric oxide synthase but not COX-2 expression. These results suggest that sesamin may be useful for improvements of the inflammatory diseases.

• Korean Journal of Agricultural Science
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• v.20 no.1
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• pp.9-17
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• 1993

Postharvest treatments such as polyethylene film wrapping, ethylene scrubber, and fruit coatings were compared to find a potential effect on the fruit quality in 'Fuji' apples during storage and simulated marketing. Polyethylene film wrapping appeared to be excellent in preventing weight loss during storage but fruits lost weight at a high rate when exposed to ambient temperature during simulated marketing. Also, surface cracking disorder was observed in parts of film-wrapped fruit under marketing condition after storage of 21 weeks. Ethylene scrubber effectively removed ethylene in polyetheylene bags but this effect reduced along with the increase of storage time. Although Prolong-treated fruits were good in appearance at 12-week storage, no beneficial effect was found on the inhibition of weight loss during storage as well as marketing. In addition, wax-like substances were accumulated on the surface after storage of 21 weeks, resulting in the reduction of marketability. Water soluble wax seemed highly effective on the inhibition of weight loss during storage and marketing. However, parts of fruit were severely injured probably due to the high concentration of wax. Undamaged fruit showed excellent appearance in shape and flesh quality even after 21 weeks of storage. Further study is required to find the proper concentration of wax.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.2 s.51
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• pp.207-212
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• 2005

Pomegranate (Punica granatum L.) which is very rich in Polyphenols and tannins was recently reported its anti-oxidant activities and phytoestrogenic activities in vivo test and many clinical studies, but the effects of them on the skin have not been reported. The experiments were tarried out in vitro to determine anti-oxidant activities of pomegranate extracts on DPPH radical scavenging assay, NBT/Xanthine Oxidase-superoxide scavenging assay, silica-induced intracellular $H_2O_2$, hydroperoxide and superoxide generation assay in RAW 264.7 cells. It showed that the methanolic extract of dried pomegranate peels have the most significant anti-oxidant activities on free radical scavenging assay and inhibitory activities on silica-induced intracellular free radical generation in RAW 264.7 cells. The concentrated juice of pomegranate showed only DPPH radical scavenging activities and inhibited hyaluronidase activity. Moreover, pomegranate seed oil inhibited specially silica-induced intracellular hydroperoxide generation in RAW 264.7 cells. These results suggest that the methanolic extract of dried pomegranate peels and pomegranate seed oil have more anti-oxidant activity than concentrated juice of pomegranate. Thus the extracts of pomegranate peels and seed oil could be developed cosmetic ingredients for anti-aging.

• Journal of Conservation Science
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• v.33 no.2
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• pp.121-130
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• 2017

The currently used wax agents for preserving outdoor metal structures, despite their advantages, have disadvantages such as low endurance and reliability. These wax agents are easily damaged by acid rain, dust, moisture in the air, yellow dust, and air pollutants, resulting in corrosion within a short period after the initial conservation treatment. In addition, aged wax can also exhibit changes in the color or gloss, and also give a sense of difference in the surface. Given these existing problems, it is necessary to develop improved materials for metal preservation. Therefore, this study analyzed the characteristics and applications of the existing wax coating agents in order to identify their disadvantages and to develop a better material for metal preservation. In this regard, this study developed a perilla oil based compound wax and conducted experiments to test its endurance. The new compound wax agent was exposed to outdoor and acid rain conditions: it showed four times and 1.5 times the endurance of the existing wax agents in outdoor and acid rain conditions, respectively. In addition, the new agent seems to be more durable and protective as evidenced by the chromaticity, polish maintenance, and contact angle results. Further, although it is 1.3-1.8 times thicker than the existing agents, the new agent shows a more even surface. Based on these findings, it can be concluded that the new compound wax agent based on perilla oil is a better alternative to the existing was coating agents.

• Journal of Life Science
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• v.33 no.5
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• pp.397-405
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• 2023

Although glutathione (GSH) has been shown to play an important role in the prevention of oxidative damage as an antioxidant, studies on immune regulation by it have not been properly conducted. In this study, we investigated whether luthione^®, a reduced GSH, has an immune enhancing effect in murine macrophage RAW 264.7 cells. The results of flow cytometry and immunofluorescence experiments indicated that luthione increased phagocytic activity, a representative function of macrophages, compared to the control cells. According to the results of the cytokine array, the expression of interleukin (IL)-5, IL-1β, and IL-27 was significantly increased in the luthione-treated cells. Luthione also enhanced the production of tumor necrosis factor-α and IL-1β through increased expression of their proteins, and increased release of the immune mediators such as nitric oxide (NO) and prostaglandin E₂ was associated with increased expression of inducible NO synthase and cyclooxygenase-2. In addition, the expression of cluster of differentiation 86, an M1 macrophage marker, was dramatically enhanced in RAW 264.7 cells treated with luthione. Furthermore, as a result of heat map analysis, we found that cytokine signaling 1/3-mediated signal transducer and activator of transcription/Janus tyrosine kinase signaling pathway was involved in the immunomodulatory effect by luthione. In conclusion, our data suggested that luthione could act as a molecular regulator in M1 macrophage polarization and enhance immune capacity by promoting macrophage phagocytic function.

• Korean Journal of Environmental Biology
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• v.26 no.1
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• pp.30-35
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• 2008

In order to investigate whether or not CCD-986sk cell line can be affected by Korean Ecklonia stolonifera Okamura, we examined the MTT assay when we treated Korean Ecklonia stolonifera Okamura extract in CCD-986sk human fibroblast cell line. The sample were tested for cell proliferation activity by means of a modification of the MTT assay. Ecklonia stolonifera Okamura extract showed significantly strong cell proliferation activity at the range of from 6.25 mg $mL^{-1}$ to 1.56 mg $mL^{-1}$ compared with control group. And in order to search for inhibition agents of skin melanin formation, we tested for inhibition effect of melanin pigmentation of Korean Ecklonia stolonifera Okamura using Clone M-3 mouse melanocyte cell lines. when we treated the extracts of Ecklonia stolonifera Okamura to the mouse melanocyte cell lines, the sample showed a significantly little formation of melanin pigments compared with control group at the only range of 200 mg $mL^{-1}$. These results suggest that extract of Korean Ecklonia stolonifera Okamura may represents an excellent candidate for inhibition of melanin pigmentation and for protection of human skin aging at in vitro level.

• Elastomers and Composites
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• v.14 no.2
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• pp.83-102
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• 1979

The purpose of this dissertation is to study some of the vulcanization characteristics of rubber-bound antioxidants such as G-1, [N-(3-methacryloyloxy-2-hydroxypropyl)-N'-phenyl-p-phenylene diamine] and acryl, acrylamide & acrylester substituted hindered phenols. The influence of these antioxidants upon vulcanization characteristics in NR and SBR compounds in the presence of vulcanizing accelerators such as MSA, TT, DM, M & D was evaluated by means of Oscillationg Disk Cure Meter. The comparison was also made between the influence of rubber-bound antioxidants and that of conventional non-reactive antioxidants such as N-alkyl substituted PADA series. Regarding the influence of reactive type antioxidant G-1 mixed with accelerator TT upon vulcanization characteristics, rapid onset of vulcanization and higher degree of cross-linking were discovered, whereas in the case of accelerator M and DM, the result was slow onset of vulcanization and lesser degree of cross-linking. The comparison of vulcanizing characteristics among acrylic substituted hindered phenols as antioxidants was made under several vulcanization accelerator systems. Under such systems, MSA-S combined accelerator caused the onset of vulcanization to slow down and lowered the degree of cross-linking. Finally in the case of hindered phenol derivatives containing longer substituent, the delayed onset of vulcanization and the lowered degree of cross-linking could be discerned.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.239-239
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• 1994

신경쇠약의 치료로 상용하며, 빈혈의 치료와 예방의 효과가 있으며, 노화방지에 도움이 되어 민간약으로는 장수(長壽)약으로 꼽히고 있는 구기자가 글루타메이트에 의한 신경독성을 차단하며, 이러한 효과는 구기자의 성분 중 betaine에 의하여 나타난다. Betaine이 어떠한 작용 기전에 의하여 글루타메이트에 의한 신경독성을 차단하는 지를 밝히기 위하여 글루타메이트가 작용하는 각기 다른 수용체인 NHDA 및 non-NMDA 수용체에 어떻게 작용하는 지를 알아보았다. 각 수용체의 선택적인 효능제인 NMDA, kainate 및 quisqualate 각각을 사용하여 인위적으로 독성을 유도시킨 후 이에 대한 betaine의 작용을 알아 본 결과 betaine은 quisqualate에 의하여 유도된 신경독성에서 모두 유사한 정도의 효과를 나타내었다. 이러한 효과는 betaine과 구조가 유사한 glycine의 다른 구조 유사체인 dimethylglycine이나 sarcosine과는 다른 작용양상을 나타내는 것이다. Dimethylglycine과 sarcosine은 kainate에 의한 독성에 대하여 미약한 효과를 나타냈으나, NMDA에 의한 독성에는 정상대조군의 LDH 값의 50% 이상에까지 이르게하는 효과를 나타내는 것으로 보아 이들이 NMDA에 의한 신경독성을 효과적으로 차단한다는 것을 암시해 준다고 할 수 있다. 그러나 betaine의 글루타메이트에 의한 신경독성을 차단하는 효과는 다른 glycine 구조 유사체처럼 glycine과 경쟁적으로 작용하여 나타나는 결과는 아니라고 여겨진다. 또한 글루타메이트에 의한 신경독성이 일어나는 기전 중의 하나가 calcium 이온의 세포내의 과도한 유입으로 인한 것으로 알려져 있으나 세포내의 calcium 이온의 양을 측정하여 본 결과 betaine은 글루타메이트로 인한 세포내 calcium 이온의 증가에 대하여 별다른 효과를 나타내지 않았다. 따라서 betaine의 글루타메이트에 의한 신경독성 차단 효과는 이미 미생물에서 보고된 바 있는 betaine의 세포내 삼투압에 대한 보호작용이나 항산화작용과 관련된 기전에 의하여 나타나는 것일 가능성이 높은 것으로 추측되어진다.

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.53-57
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• 2018

고도의 산업화에 따라 도시의 성장과 인구의 밀집으로 인하여 물의 수요가 증가하고 이에 따라 각종 오염원의 유입이 증가함에 따라서 적절한 수질 관리에 대한 필요성이 대두되고 있다. 그리고 산업이 다양화됨에 따라 배출되는 하 폐수의 특성도 다양해져 기존의 수질처리장은 효율적인 처리를 하는데 있어 많은 어려움이 발생되고 있다. 굴 패각은 하수처리의 담체로 이용할 경우 살수여상의 문제점 중 하나로 나타나는 막힘 현상을 다소 감소시킬 수 있고, 다공질체로서 표면적이 불규칙하고 비표면적이 크기 때문에 반응기내에 공기를 원활하게 공급할 수 있으며, 중금속 이온과 유기물에 대한 흡착 효율이 뛰어나 미생물이 쉽게 부착, 성장할 수 있는 장점을 가지고 있다. 천연광물질은 전 세계적으로 발견되어 활용된 역사가 짧지만, 그 특징은 산소결핍보충, 유해원소 흡착 성능, 반도체 작용, 인체 면역력 강화, 바이러스성 질병의 예방, 중금속 해독 등의 다양한 특징으로 환경호르몬 제고, 음이온 원적외선 발생으로 세포의 노화방지, 인체 노폐물 제거와 같은 특징을 지닌 매우 유용한 자원으로 확인되고 있으며, 수질정화 능력이 탁월한 것으로 보고 있다. 이에 본 연구는 어촌폐기물인 굴 패각과 수질정화에 탁월한 천연 광물질을 혼합하여 경제적이고, 효율성이 높은 다공성 수질개선 담체를 개발하고, 수목여과용 비점오염시설 장치에 적용하여 혼합 담체가 수처리 시설의 담체로서 사용이 가능한지, 수목여과용 비점오염시설 장치에 적용할 수 있는지 검토하고자 수행하였다. 이 연구는 2017년 중소기업청 기술개발사업에서 연구지원을 받아 연구되었습니다.

• Korean Journal of Food Science and Technology
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• v.41 no.6
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• pp.700-705
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• 2009

Several studies have demonstrated that chlorophyll has many beneficial properties, including anti-inflammatory, anti-tumor, and antioxidant properties. Chlorophyll has been also shown to have an excellent chemopreventive potential against skin tumor. Its preventive mechanism against skin tumor, however, has not been examined in detail. Furthermore, the effects of chlorophyll on UVB-induced cellular responses have not been investigated. Solar UV radiation, in particular its UVB component, is the primary cause of many adverse biological effects, which is responsible for the photoaging and skin cancer. We investigated the preventive effects of chlorophyll a on UVB-mediated responses in human immortalized HaCaT kerationocytes and normal human fibroblast. We found that treatment of chlorophyll a markedly inhibited UVB-induced generation of reactive oxygen species and lipid peroxidation. Chlorophyll a also prevented UVB-induced MMP-1 expression and MMP-2 activation and increased Type I pN collagen synthesis. These results suggest that chlorophyll a is a potent candidate for the prevention and treatment of UVB-induced skin cancer and photoaging.