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Anti-oxidant Activities of Punica granatum Extracts  

Roh, Bo-Kyung (The Condition Physiology Classroom Chungang University)
Kim, Jee-Young (The Condition Physiology Classroom Chungang University)
Kim, Ju-Yon (Department of Beauty art school, Seokyeong University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.31, no.2, 2005 , pp. 207-212 More about this Journal
Abstract
Pomegranate (Punica granatum L.) which is very rich in Polyphenols and tannins was recently reported its anti-oxidant activities and phytoestrogenic activities in vivo test and many clinical studies, but the effects of them on the skin have not been reported. The experiments were tarried out in vitro to determine anti-oxidant activities of pomegranate extracts on DPPH radical scavenging assay, NBT/Xanthine Oxidase-superoxide scavenging assay, silica-induced intracellular $H_2O_2$, hydroperoxide and superoxide generation assay in RAW 264.7 cells. It showed that the methanolic extract of dried pomegranate peels have the most significant anti-oxidant activities on free radical scavenging assay and inhibitory activities on silica-induced intracellular free radical generation in RAW 264.7 cells. The concentrated juice of pomegranate showed only DPPH radical scavenging activities and inhibited hyaluronidase activity. Moreover, pomegranate seed oil inhibited specially silica-induced intracellular hydroperoxide generation in RAW 264.7 cells. These results suggest that the methanolic extract of dried pomegranate peels and pomegranate seed oil have more anti-oxidant activity than concentrated juice of pomegranate. Thus the extracts of pomegranate peels and seed oil could be developed cosmetic ingredients for anti-aging.
Keywords
anti-oxidant activities; phytoestrogenic; pomegranate; anti-aging;
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  • Reference
1 박수남, Skin aging and reactive oxygen species, 논문집, 서울산업대학교 (1992)
2 M. I. Gil, F. A. Tomas-Barberan, B. Hess-Piearce, D. M. Holcroft, and A. A. Kedar Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing, J. Agric. Food Chem., 10, 4581 (2000)
3 S. Kawaii and E. P. Lansky. Differentiation-promoting activity of pomrgranate (Punica granatum L.) fruit extracts in HL-60 human promyelocytic leukemia cells, J. Med. Food, 7, 13 (2004)   DOI   ScienceOn
4 F. Afaq, M. Saleem, C. Krueger, J. Reed, and H. Mukhtar, Anthocyanins and hydrolyzways tanninsl rpomegranatits skin fruit extract modulates MAPKs and NF-kB pathways and inhibits skin tumorigenesis in CD-1 mice, Int. J. Cancer (In Press) (2004)
5 S. Y. Schubert, I. Neeman, and N. Resnick. A novel mechanism for the inhibition of NF-kappaB activation in vascular endothelial cells bynatural antioxidants, FASEB J., 16, 1931 (2002)   DOI
6 최원균, 이란산 흑석류 농축액과 그 제품의 성분 및 함유된 Phyto 에스트로겐효과, 한국식품영양학회지, 2, 119 (2002)
7 심선미, 석류 추출성분의 항발암 활성 및 Phytoestrogen 효과, 신라대학교 대학원 (2001)
8 박미라, 석류가 갱년기 장애를 유도한 흰 쥐의 혈중지질 및 결합조직의 collagen 함량에 미치는 영향, 신라대학교 대학원 (2002)