• Title/Summary/Keyword: 노인 낙인

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Identifying Genes Related with Self-thinning Characteristics in Apple by Differential Display PCR (Differential Display PCR을 이용한 사과 자가적과성 연관 유전자 탐색)

  • Kim, Se Hee;Heo, Seong;Shin, Il Sheob;Kim, Jeong-Hee;Cho, Kang-Hee;Kim, Dae-Hyun;Hwang, Jeong Hwan
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.565-573
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    • 2010
  • Thinning of apple fruitlets is one of the most laborious and important works for the improvement of fruit quality and for the promotion of sufficient flower bud formation to prevent alternate bearing in commercial cultivars. Lateral fruits of self-thinning apple cultivars fall naturally within 30 days after full bloom and only central fruit remains to mature. Differences of gene expression between central fruit and lateral fruit were investigated by differential display (DD) PCR. Partial cDNAs of 30 clones from the central fruit and 24 clones from the lateral fruit were selected for nucleotide sequence determination and homology searches. The levels of transcripts coding for proteins involved in pathogenesis related proteins, senescence, temperature stress, protein degradation, fruit browning, sorbitol metabolism were significantly higher in pedicels of lateral fruit than in pedicels of central fruit. On the other hand, the up-regulation of proteins involved in anthocyanin and flavanol biosynthesis and ethylene synthesis were observed in pedicels of central fruit. In Real time PCR analysis, cytochrome P450 gene was confirmed as showing a higher expression level in lateral fruit than in central fruit. The results of this study indicate that differentially expressed genes are related to self-thinning characteristics in apple tree.

Body Movement as Identity: The Meaning of the 'Bodily Culture' of Older Men at Jongmyo Park (정체성으로서의 몸짓: 종묘공원 노년 남성들의 '몸짓문화'의 의미)

  • Chung, Gene-Woong
    • 한국노년학
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    • v.31 no.1
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    • pp.157-170
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    • 2011
  • The purpose of this article is to examine how the 'bodily culture' of older men who gather at Jongmyo Park serves to express and solidify their sense of a enduring self. The research was carried out based on the anthropological methods of participant observation and in-depth interviews at Jongmyo park and its adjacent areas. In later life, the image of a continuous self becomes harder to achieve due to the ageism prevalent in modern societies. The situation is worse at Jongmyo park since the park itself has been stigmatized as "the extraterritorial zone for the old" by the media due to the boisterous atmosphere of the park. It is dubbed as a place for those who have nowhere else to go. The singing and dancing, which used to be the dominant forms of activities at the park among older men until the 'sanctification' project propelled by the government, serves as an antidote against the ageist tendency to subjugate them. The 'bodily culture' embodies these men's image of themselves as a continuous being which has not been tarnished by the passage of time. The approach of 'body hermeneutics' is required to interpret the experiences of the body in later life.