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Ultrastructural Variations on the Micropyle of Blacktip Grouper, Epinephelus Fasciatus before and after Artificial Fertilization (홍바리, Epinephelus fasciatus의 인공수정 전.후 난문의 미세구조적 변화)

  • Kim, Seong-Hoon;Lee, Chi-Hoon;Ju, Hea-Sung;Kim, Hyung-Bae;Lee, Young-Don
    • Applied Microscopy
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    • v.41 no.2
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    • pp.123-128
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    • 2011
  • The micropyles on the prefertilized and artificial fertilized eggs of Epinephelus fasciatus were investigated using scanning electron microscope (SEM). The micropyles ($6.6{\pm}0.41\;{\mu}m$) of E. fasciatus eggs were found in the animal pole and theirs shape were observed as a flat crateriform of cylindrical shape. The micropylar vestibule arranged by 6~7 thickened spiral annuli on the ridge and contributed to differentiate and form fertilization cone for blocking to polyspermy by presenting swollen vestibule structure. As E. fasciatus eggs was pelagic, so chorionic surface was an uneven structures such as circular and fillar form nodules. Especially, various pores (0.15~0.55 ${\mu}m$, 230~270 pores) distributed at the only around micropyles, those pores radiately exhibited regular projection structures showing gill filament-shape. These ultrastructural characters of E. fasciatus eggs can be utilized in a taxonomical cue of grouper species.

Morphological Study of the Suction Trap in Aquatic Utricularia japonica (수생형 통발(Utricularia japonica)의 흡입식 포충낭 형태 연구)

  • Kim, In-Sun
    • Applied Microscopy
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    • v.40 no.2
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    • pp.109-116
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    • 2010
  • Morphology and microstructure of the suction trap in aquatic Utricularia japonica were examined using scanning electron microscopy. Branched stems bear numerous suction traps without root formation. The traps are derived axillary from the node, and their antennae and appendages extend in a peculiar fashion. The trap walls are thin, two-celled, parenchyma tissue and simple, small glands are scattered in both internal and external surface of the trap. The entrance of the trap is surrounded by one pair of dorsal antennae and ventral appendages, where the former guides the prey to the entrance. Trap door is situated below the entrance and numerous sessile and stalked capitate trichomes cover the entrance and even on the door surface. The capitate trichomes are secretory, but four trigger hairs formed on the central areas of the door are not. They are believed to function in activating and tripping the trap door. A specialized region of the threshold come in contact with the lower portion of the door upon closing. The secretory capitate trichomes near this region are responsible for producing and secreting a mucilage-like substance which composes the velum. Two-armed bifid glands are located in the interior side of the threshold, while four-armed quadrifid glands are considerably numerous occurring over the entire inner trap wall. Bifid and quadrifid glands develop semi-spherical basal cells that connect them to the inner wall surface. Antennae, trigger hairs, capitate trichomes, bifid and quadrifid glands are more important structures in the carnivory of U. japonica.

Thermal Analysis of a Retrievable CANDU Spent Fuel Disposal Tunnel (회수 가능 CANDU 사용후핵연료 처분터널에 대한 열 해석)

  • Cha, Jeong-Hun;Lee, Jong-Youl;Choi, Heui-Joo;Cho, Dong-Keun;Kim, Sang-Nyung;Youn, Bum-Soo;Ji, Joon-Suk
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.6 no.2
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    • pp.119-128
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    • 2008
  • Thermal assessment of a new CANDU spent fuel disposal system, which improves the retrievability of the spent fuel and enhances the densification factor compared with the Korean Reference disposal System, is carried out in this study. The canisters for CANDU spent fuels are stored for long term and cooled by natural convection in the proposed disposal system for the retrievability. The steady state thermal analyses for proposed CANDU disposal system are carried out with the ANSYS 10.0 CFX code. The thermal analyses are performed through two steps. At the first step, the sensitivity of the disposal tunnel spacing is analysed. The differences of maximum temperatures by several tunnel spacings are calculated at three points in the disposal tunnel. The result shows that the differences of the temperature at the three points are almost negligible because 99% of the decay heat is removed by natural convection. At the second procedure, 60m tunnel spacing with a ventilation system instead of natural convection is considered. The result is applied to the calculation of the canister surface temperature in disposal tunnel as boundary conditions. Consequently, the average and the maximum surface temperature of disposal canisters are $79.9^{\circ}C$ and $119^{\circ}C$, respectively. The inner maximum temperature of a basket in the disposal canister is calculated as $140.9^{\circ}C$. The maximum temperature of the basket meets the thermal requirement for the CANDU spent fuel cladding.

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Blood Vessel Strain Imaging Using Linear Array Transducer (선형 트랜스듀서를 이용한 혈관 변형률 영상법)

  • Ahn, Dong-Ki;Jeong, Mok-Kun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.3
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    • pp.880-890
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    • 2010
  • The intrasvascular ultrasound (IVUS) imaging technique is used to diagnose cerebrovascular diseases such as stroke. Recently, elasticity imaging methods have been investigated to diagnose blood clots attached to blood vessel intima. However, the IVUS imaging technique is an invasive method that requires a transducer to be inserted into blood vessel. In this paper, strain images are obtained of blood clots attached to blood vessel intima with data acquired from outside the blood vessel using a linear array transducer. In order to measure the displacement of blood vessel accurately, experimental data are acquired by steering ultrasound beams so that they can intersect the blood vessel wall at right angles. The acquired rf data are demodulated to the baseband. The resulting complex baseband signals are then processed by an autocorrelation algorithm to compute the blood vessel movement and thereby produce strain image. This proposed method is verified by experiments on a plastic blood vessel mimicking phantom. The efficacy of the proposed method was verified using a home-made blood vessel mimicking phantom. The blood vessel mimicking phantom was constructed by making a 6 mm diameter hollow cylinder inside it to simulate a blood vessel and adhering 2 mm thick soft plaque to the inner wall of the hollow cylinder. The RF data were acquired using a clinical ultrasound scanner (Accuvix XQ, Medison, Seoul. Korea) with a 7.5 MHz linear array transducer by steering ultrasound beams in steps of $1^{\circ}$ from $-40^{\circ}$ to $40^{\circ}$ for a total of 81 angles. Experimental results show that the plaque region near the blood vessel wall is softer than background tissue. Although the imaging region is restricted due to the limited range of angles for which scan lines are perpendicular to the wall, the feasibility of strain imaging is demonstrated.

Numerical Analysis of the Temperature Distribution Considering the Wall Thermal Conductivity in Compartment Fire (구획 화재 시 벽면의 열적 특성을 고려한 온도분포 해석결과)

  • You, Woo Jun;Ko, Kwon Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.2
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    • pp.644-648
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    • 2019
  • This study examined effects of the wall thermal conductivity coefficients on the thermal fluid phenomenon of a compartment fire. The reduced scale compartment was 0.4 m in width, 0.6 m in length and 0.6 m in height with a fire-board, which has a thermal conductivity coefficient of $0.18W/m{\cdot}K$. The local temperature at a 0.37 m height and the overall heat release rate were measured under the following experiment conditions: a $0.12m^2$ opening area and $0.01m^2$ pool size of a gasoline fire. The numerical results obtained by the Fire Dynamic Simulation were compared with the experimentally measured temperature. The deviations were within 10 % in the period of the steady state for maximum heat release rate (4.8 kW). The numerical results show that the average temperature of the compartment wall decreases by approximately 71 % with increasing thermal conductivity coefficient from $0.1W/m{\cdot}K$ to $100.0W/m{\cdot}K$ on the fixed heat release rate.

A Study on Injection Nozzle and Internal Flow Velocity for Removing Air Bubbles inside the Sample Tanks during Hydraulic Rupture Test (수압파열시험 시 시료 탱크 내부 기포 제거를 위한 주입 노즐 및 내부 유속 연구)

  • Yeseung, Lee;Hyunseok, Yang;Woo-Chul, Jung;Dong Hoon, Lee;Man-Sik, Kong
    • Journal of the Korean Institute of Gas
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    • v.26 no.6
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    • pp.9-15
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    • 2022
  • In order to verify the durability of the high-pressure hydrogen tank in the operating pressure range, a hydraulic rupture test should be performed. However, if the bubbles generated by the initial injection process of water are attached to the inner wall of the tank and remain, a sudden pressure change of the bubbles during the rupture of the pressurized tank may cause shock and noise. Therefore, in this study, the flow velocity required to remove the bubbles remaining on the inner wall of the tank was predicted through simplified formulas, and the shape of the injection nozzle to maintain the flow velocity was determined based on the shape of the hydrogen tank for the hydrogen bus. In addition, a numerical model was developed to predict the change in flow velocity according to the inlet pressure, and an experiment was performed through a model tank to prove the validity of the prediction result. As a result of the experiment, the flow velocity near the tank wall was similar to the predicted value of the analysis model, and when the inlet pressure was 1.5 to 5.5 bar, the minimum size of the removable bubble was predicted to be about 2.2 to 4.6 mm.

Electrochemical Mass Transport Control in Biomimetic Solid-State Nanopores (생체모사형 나노포어를 활용한 전기화학 기반 물질전달 조절 시스템)

  • Soongyu Han;Yerin Bang;Joon-Hwa Lee;Seung-Ryong Kwon
    • Journal of the Korean Electrochemical Society
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    • v.26 no.4
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    • pp.43-55
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    • 2023
  • Mass transport through nanoporous structures such as nanopores or nanochannels has fundamental electrochemical implications and many potential applications as well. These structures can be particularly useful for water treatment, energy conversion, biosensing, and controlled delivery of substances. Earlier research focused on creating nanopores with diameters ranging from tens to hundreds of nanometers that can selectively transport cationic or anionic charged species. However, recent studies have shown that nanopores with diameters of a few nanometers or even less can achieve more complex and versatile transport control. For example, nanopores that mimic biological channels can be functionalized with specific receptors to detect viruses, small molecules, and even ions, or can be made hydrophobic and responsive to external stimuli, such as light and electric field, to act as efficient valves. This review summarizes the latest developments in nanopore-based systems that can control mass transport based on the size of the nanopores (e.g., length, diameter, and shape) and the physical/chemical properties of their inner surfaces. It also provides some examples of practical applications of these systems.

In Vitro Culture of Endothelial Cell and Smooth Muscle Cell for Studying Vascular Diseases

  • Kim, Joo-Young
    • Journal of Yeungnam Medical Science
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    • v.27 no.2
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    • pp.91-97
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    • 2010
  • Endothelial cells play a key role in pathological processes such as cancer cell metastasis, atherosclerosis, and diabetic retinopathy. Vascular smooth muscle cells directly involve in the formation of atheroma in atherosclerosis. Some kinds of the endothelial cells are simply harvested from the umbilical veins, the tunica intima of aortic walls, the retina using various enzymes solutions. Those purely isolated cells provide a powerful tool in vitro studies of the endothelial cell related diseases. In this context, the cultured smooth muscle cells after the isolation from the tunica media of aortic walls are also used for elucidating the pathogenesis of atherosclerosis. Here, I briefly introduce articles that include the isolation of human umbilical vein endothelial cells(HUVEC), aortic endothelial and smooth muscle cells, retinal microvascular endothelial cells(RMEC), as well as the diseases' applications of these cells.

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Repeatability of Methane Hydrate Formation (메탄하이드레이트 생성의 반복성에 대한 연구)

  • Park, Sung-Seek;Kim, Nam-Jin
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.06a
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    • pp.218.1-218.1
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    • 2010
  • 천연가스를 대체하며 21세기 신 에너지원으로 기대되고 있는 메탄 하이드레이트가 주목을 받게된 것은 1930년대 시베리아의 화학 플랜트에서 고압의 천연가스 수송용 파이프라인이 막히는 사고가 빈번하게 발생하여 그 원인을 조사한 결과, 파이프 내에서 가스와 물이 결합하여 하이드레이트를 형성하고, 그것이 파이프의 내벽에 부착되어 파이프를 막고 있다는 것으로 밝혀지면서 천연가스 하이드레이트가 주목을 받게 되었다. 또한 메탄 하이드레이트의 경우 46개의 물분자에 8개의 메탄가스 분자가 포획된 구조로, 그 분자식은 $CH_4{\cdot}5.75H_2O$이다. 따라서 메탄가스와 물의 이론적 용량비가 216:1로써, 표준상태에서 $1m^3$의 메탄 하이드레이트는 $172m^3$의 메탄가스와 $0.8m^3$의 물로 분해된다. 만약 이와 같은 특징을 역으로 이용할 경우 메탄을 주성분으로 하는 천연가스를 물에 포집시켜 인공적으로 하이드레이트를 제조할 수 있기 때문에 천연가스 수송 및 저장의 수단으로써 그 중요성이 커지고 있으며, 액화수송보다 18-24%의 비용절감이 이루어진다고 보고하였다. 그러나 인공적으로 메탄 하이드레이트를 제조할 경우 가스 포집율의 예측이 매우 어려운 것으로 알려져 있다. 따라서 본 연구에서는 동일한 조건에서 메탄 하이드레이트 형성의 반복성 실험을 10회 수행한 결과 과냉도가 클수록 최대최소차이가 줄었고 또한 교반을 시킬 경우도 최대최소차이가 줄어 들었다.

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An unified rough and finish cut algorithm for NC machining of free form pockets with general polygon - Part 2. Experiment (일반적인 내벽을 가진 자유바닥 곡면 파켓의 NC 가공을 위한 단일화된 황삭과 정삭 알고리즘 - Part 2. Experiment)

  • Choi, Yong-Hoon;Kim, Sang-Jin
    • Journal of Korea Society of Industrial Information Systems
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    • v.12 no.1
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    • pp.46-53
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    • 2007
  • NC (Numerical Control) code for the tool path needs to be generated efficiently for machining of free form pockets with arbitrary wall geometry on a three axis CNC machine. The unified rough and finish cut algorithm and the tool motion is graphically simulated in Part 1. In this paper, a grid based 3D navigation algorithm simulated in Part 1 for generating NC tool path data for both linear interpolation and a combination of linear and circular interpolation for three-axis CNC milling of general pockets with sculptured bottom surfaces is experimentally performed and verified.

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