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Ultrastructural Variations on the Micropyle of Blacktip Grouper, Epinephelus Fasciatus before and after Artificial Fertilization  

Kim, Seong-Hoon (Marine and Environmental Research Institute of Jeju National University)
Lee, Chi-Hoon (Marine and Environmental Research Institute of Jeju National University)
Ju, Hea-Sung (Marine and Environmental Research Institute of Jeju National University)
Kim, Hyung-Bae (Department of Marine Bio-resources, Gangwon Provincial University)
Lee, Young-Don (Marine and Environmental Research Institute of Jeju National University)
Publication Information
Applied Microscopy / v.41, no.2, 2011 , pp. 123-128 More about this Journal
Abstract
The micropyles on the prefertilized and artificial fertilized eggs of Epinephelus fasciatus were investigated using scanning electron microscope (SEM). The micropyles ($6.6{\pm}0.41\;{\mu}m$) of E. fasciatus eggs were found in the animal pole and theirs shape were observed as a flat crateriform of cylindrical shape. The micropylar vestibule arranged by 6~7 thickened spiral annuli on the ridge and contributed to differentiate and form fertilization cone for blocking to polyspermy by presenting swollen vestibule structure. As E. fasciatus eggs was pelagic, so chorionic surface was an uneven structures such as circular and fillar form nodules. Especially, various pores (0.15~0.55 ${\mu}m$, 230~270 pores) distributed at the only around micropyles, those pores radiately exhibited regular projection structures showing gill filament-shape. These ultrastructural characters of E. fasciatus eggs can be utilized in a taxonomical cue of grouper species.
Keywords
Blacktip grouper; Epinephelus fasciatus; Micropyle; SEM;
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1 Riehl R, Kock KH: The surface structure of Antarctic fish eggs and its use in identifying fish eggs from the southern ocean. Polar Biol 43 : 617-620, 1989.
2 Riehl R, Kokoscha M: A unique surface pattern and micropylar apparatus in the eggs of Luciocephalus sp. (Perciformes, Luciocephalidae). J Fish Biol 43 : 617-620, 1993.   DOI   ScienceOn
3 Rizzo E, Sato Y, Barreto BP, Godinho HP: Adhesiveness and surface patterns of eggs in neotropical freshwater teleosts. J Fish Biol 61 : 615-632, 2002.   DOI   ScienceOn
4 Stanley HP: Fine structure of spermatogenesis in the elasmobranch fish Squalussuckleyi. J Ultrastruct Res 36 : 86-102, 1971.   DOI
5 Vorobyeva EI, Markov KP: Specific ultrastructural features of eggs of Acipenseridae in relation to reproductive biology and phylogency. J Ichthyol 39 : 157-169, 1999.
6 Kim DH, Reu DS, Deung YK: Ultrastructure of the fertilized egg envelope from Dark sleeper, Eleotrididae, Teleost. Kor J Electron Microscopy 32 : 39-44, 2002. (Korean)
7 Mekkawy IAA, Osman AGH: Ultrastructural studies of the morphological variations of the egg surface and envelopes of the African catfish, Clarias gariepinus (Burchell, 1982) before and after fertilisation, with a discussion of the fertilization mechanism. Sci Mar 702 : 23-40, 2006.
8 Mikodina EV: Surface structure of the egg of teleostean fishes. Voprosy Ikhtiologii 1 : 106-113, 1987.
9 Riehl R: Micropyle of some salmonids and coregonids. Environ Biol Fish 5 : 59-66, 1980.   DOI   ScienceOn
10 Kim DH, Deung YK, Kim HY, Reu DS: Ultrastructure of the fertilized egg envelope from long nose barbel, Cyprinidae, teleost. Kor J Electron Microscopy 31 : 85-90, 2001. (Korean)
11 Kobayashi W, Yamamoto TS: Fine structure of micropylar apparatus of the chum salmon egg with a discussion of the mechanism for blocking polyspermy. J Exp Zool 217 : 265-275, 1981.   DOI
12 Laale HW: The perivitelline space and egg envelopes of bony fishes: a review. Copeia 2 : 210-226, 1980.
13 Li YH, Wu CC, Yang JS: Comparative ultrastructural studies of the zona radiata of marine fish eggs in three genera in Peciformes. J Fish Biol 56 : 615-621, 2000.   DOI   ScienceOn
14 Linhart O, Kudo S: Surface structure of paddlefish eggs before and after fertilization. J Fish Biol 51 : 573-582, 1997.
15 Hart NH: Fertilization in teleost fishes: mechanisms of sperm-egg interactions. Int Rev Cytol 121 : 1-66, 1990.
16 Esmaeili HR, Johal MS: Ultrastructural features of the egg envelope of silver carp, Hypophthalmichtys molitrix (Osteinchthys, Cyprinidae). Environ Biol Fish 72 : 373-377. 2005.   DOI   ScienceOn
17 Giulianini PG, Ferrero EA: Ultrastructural aspects of the ovarian follicle and egg envelope of the sea-grass goby Zosterisessor ophiocephalus (Osteichthyes, Gobiidae). Ital J Zool, 68 : 29-37, 2000.
18 Guraya SS: The cell and molecular biology of fish oogenesis. Karger, New York, pp. 141-147, 1986.
19 Hirai A, Yamamoto ST: Micropyle in the developing of the anchovy, Engraulis japonica. Japan J Ichthyol 33 : 62-66, 1986.
20 Chen KC, Shao KT, Yang JS: Using micropylar ultrastructure for species identification and phylogenetic inference among four species of Sparidae. J Fish Biol 55 : 288-300, 1999.   DOI   ScienceOn
21 Cherr GN, Clar WHJr: An egg envelope component induces the acrosome reaction in sturgeon sperm. J Exp Zool 234 : 75-85, 1985.   DOI   ScienceOn
22 Chiou LM, Chung MC, Tung PG, Hsu T, Yang JS: The use of egg chorion glycoprotein of Epinephelus malabaricus for egg identification. J Fish Biol 65 : 1614-1621, 2004.   DOI   ScienceOn
23 Dettlaff TA, Ginsburg AS, Schmalhausen O: Sturgeon Fishes: Developmental Biology and Aquacutlrue. Springer-Verlag, Berlin, p. 300, 1993.Deung YK, Kim DH, Reu DS: Ultrastructure of the fertilized egg envelop in Pale chub Cyprinidae, teleost. Kor J Electron Microscopy 30 : 321-326, 2000. (Korean)
24 Breining T, Britz R: Egg surface structure of clingfish species, using scanning electron microscopy. J Fish Biol 56 : 1129-1137, 2000.   DOI   ScienceOn
25 Berois N, Arezo MJ, Chalar C, Brauer MM, Barros C: Ultrastructure and protein composition of the oocyte envelope in the white mouth croaker (Micropogonias furnieri, Desmarest, 1823, Sciaenidae, Perciformes). J Appl Ichthyol 23 : 34-39, 2007.   DOI   ScienceOn
26 Bless R, Riehl R: Biology and egg morphology of the Damatian barbelgudgeon, Aulopyge huegeli, an endangered endemic species in Croatia. Environ Biol Fish 63 : 451-456, 2002.   DOI   ScienceOn