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An Analysis of the Heritability of Phenotypic Traits Using Chloroplast Genomic Information of Legume Germplasms (엽록체 유전정보를 이용한 두류 유전자원 형태적 형질의 유전력 분석)

  • Dong Su Yu;Yu-Mi Choi;Xiaohan Wang;Manjung Kang
    • Korean Journal of Plant Resources
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    • v.36 no.4
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    • pp.369-380
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    • 2023
  • Developing and breeding improved legume-based food resources require collecting useful genetic traits with heritability even though requiring some time-consuming, costly, and labor intensive. We attempted to infer heritability of nine genetic traits-days to flowering, days to maturity, period from flowering to maturity, the number of seeds per pod, 100-seeds weight, and four contents such as crude protein, crude oil, crude fiber, and dietary fiber-using 455 homologous chloroplast gene sets of six species of legumes. Correlation analysis between genetic trait differences and phylogenetic distance of homologous gene sets revealed that days to flowering, the number of seeds per pod, and crude oil content were influenced by genetic factors rather than environmental factors by 62.86%, 69.45%, 57.14% of correlated genes (P-value ≤ 0.05) and days to maturity showed intermediate genetic effects by 62.42% (P-value ≤ 0.1). The period from flowering to maturity and 100-seeds weight showed different results compared to those of some previous studies, which may be attributed to highly complicated internal (epistatic or additive gene effects) and external effects (cultural environment and human behaviors). Despite being slightly unexpected, our results and method can widely contribute to analyze heritability by including genetic information on mitochondria, nuclear genome, and single nucleotide polymorphisms.

Analysis on the Factors affecting the Ruling on Construction Project Litigation - Focused on the Union Establishment of the Urban and Housing Redevelopment Project - (건설사업의 소송판결에 영향을 미치는 요인에 관한 연구 - 도시정비사업 조합설립인가 사건을 중심으로 -)

  • Kim, Yohan;Jung, Boseon;Lee, Sangyoub
    • Korean Journal of Construction Engineering and Management
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    • v.21 no.1
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    • pp.40-49
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    • 2020
  • This study analyzed the factors affecting the ruling of litigation on the housing reconstruction and housing redevelopment project based on the cross analysis and logistic regression analysis. According to cross analysis result, the defensive process prerequisite group was showed significant in many variables which were past legal relation, no ownership or association member status, double lawsuit, abuse of lawsuit right·litigation trust, existence of claim-preclusion, no standing to sue·no standing to be sued, lapse of litigation period, no legal interest, no own defect of approval. On the other hand, the offensive cause of action group was found to have no significant variable. According to logistic regression result, the defensive process prerequisite group was showed significant in many variables which were past legal relation, no standing to sue·no standing to be sued, no ownership or association member status, double lawsuit, no own defect of approval, abuse of lawsuit right·litigation trust, existence of claim-preclusion. Meanwhile, the offensive cause of action group was showed significant in only one variable that was defect in relation with articles of association. Overall, it is noteworthy that the offensive cause group showed very low significant results compared with the defensive process prerequisite group.

The Relationship between Local Fiscal Indices and Standardized Mortality rate (지역 재정지표와 표준화 사망률의 관련성)

  • Han, Ji-Yeon;Na, Bak-Ju;Lee, Moo-Sik;Hong, Jee-Young;Lim, Nam-Gu
    • Proceedings of the KAIS Fall Conference
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    • 2010.05b
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    • pp.1072-1076
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    • 2010
  • 본 지역 재정지표와 표준화사망률간의 관계에 대한 것으로, 연구대상지역은 1998년부터 2007년까지의 전국 232개 시 군 구이며 이를 5개 광역권과 4개 도시 종류에 따라 분류하였다. 지역 재정지표는 1인당 지방세부담액과 재정자립도, 재정자주도, 의존재원비율을 활용하였고, 지역 총사망률은1998년에서 2007년까지의 통계청 사망 원자료 상의 사망자수를 분자로, 주민등록인구를 분모로 직접 표준화법을 사용하여 연구대상 지역의 성 연령표준화사망률을 산출하였다. 자료의 분석은 SPSS 12.0K를 이용하여 상관분석, 일원배치분산분석(Tukey b 사후검정) 및 회귀분석을 실시하였다. 주요 결과로는 첫째, 재정지표와 표준화사망률간의 상관분석을 실시하여 연도별로 계수 값을 구한 결과 1인당 지방세부담액을 제외하고 재정자립도, 재정자주도, 의존재원비율 모두 남자, 여자, 전체 모두가 전 연도에 걸쳐 상관계수 값이 통계적으로 유의하였으며, 남자가 여자보다 높은 상관관계를 보였다. 둘째, 재정자립도, 재정자주도 각각을 표준화사망률과 단순 회귀분석을 실시한 결과, 표준화사망률 남자, 여자, 전체가 전 연도에서 통계적으로 유의하였고, 재정자립도와 재정자주도가 낮을수록 사망률이 높은 것으로 나타났다. 셋째, 광역권역, 도시 종류까지 고려한 재정지표의 다중회귀분석을 실시한 결과, 1인당 지방세부담액과 의존재원비율, 광역권역과 도시 종류에 따른 지역을 고려하고도 재정자주도의 효과는 전체사망과 남자, 여자, 전 연도에 걸쳐 모두 통계적으로 매우 유의하여 재정자주도가 높을수록 사망률이 낮은 것으로 나타났고 이런 경향은 여자보다 남자에서 더욱 강하게 나타났다. 넷째, 광역권별 분석의 경우, 충청권은 수도권에 비해서 표준화사망률에서 유의한 차이는 없었으며 호남권과 영남권은 전체 표준화사망률의 경우 전체 연도의 절반 이상에서 수도권에 비해서 통계적으로 유의하게 높았고, 남자와 여자에서는 이런 경향이 약해졌다. 강원 제주권은 전체 사망에서 수도권에 비해 전체 연도의 절반 이상이 유의하게 사망률이 낮았으며, 여자도 이와 비슷한 양상을 보여주었다. 다섯째, 도시 종류에 따른 분석에서 대도시에 비해 중소도시는 통계적으로 유의한 차이는 없었으나, 전 연도에 걸쳐 도농통합도시와 군지역은 대도시에 비해 통계적으로 사망률이 높았다. 여섯째, 전 연도에 걸쳐 의존재원비율이 높아질수록 사망률이 유의하게 높아졌다. 이는 남자, 여자 모두에서 유사하게 나타났다. 마지막으로 연도별 분석 이후 1998년에서 2007년 전체 다중 회귀분석을 실시한 결과 전체 사망과 여자의 경우 1인당 지방세부담액을 제외한 모든 변수에서 통계적으로 유의하였다. 지역의 재정력이 성 연령 표준화사망률에 영향을 미치는 것으로 파악되었는데 이를 단서로 지역의 건강 격차가 발생하는 원인과 기전을 밝히기 위해 향후 보다 면밀한 후속 연구가 이뤄져야 하겠고 지역 간 건강 격차를 완화하기 위한 여러 방법론적 고찰 안에 지역간 재정력의 격차를 완화하려는 정책적인 접근도 필요할 것으로 사료된다.

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Comparative Study of Native Flowers for Anti-oxidative Effects in Korea (국내 자생 꽃 품종에 따른 항산화활성 비교)

  • Sa, Yeo Jin;Park, Jong Hyuk;Kim, Dong Hyun;Yeom, Myeong Hun;Cho, Jun Cheol;Kwon, Yong Soo;Kim, Myong Jo
    • Korean Journal of Plant Resources
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    • v.26 no.4
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    • pp.433-440
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    • 2013
  • Nine kinds of flowers were selected by its antioxidative activities evaluated. DPPH(1,1-diphenyl-2-picryl hydrazil), reducing power, total phenol contents, total flavonoid contents, and antimicrobial activity inhibitory effects of nine natural flower varieties were examined using ethanol extract (80%, v/v). DPPH radical scavenging of Agastache rugosa (fisch.&Mey.) kuntze ($IC_{50}=74.6{\mu}g/mL$) and solidago virga-aurea var. asiatica ($IC_{50}=99.6{\mu}g/mL$) showed higher antioxidant activity compared with those of the other varieties. Reducing power of Agastache rugosa (fisch.&Mey.) kuntze ($OD_{700}=1.0$) had higher antioxidant activity. Agastache rugosa (fisch.&Mey.) kuntze showed the highest content of total phenol (134.6 mg GAE/g). However, total flavonoid (554.6 mg QE/g) exhibited the lowest. These results suggest that nine kinds of flower with 80% ethanol extracts have significant antioxidant activity.

Variation of Antioxidant and Anticancer Activities of Hull and Bran Extracts in Different Colored Rices (종피색이 다른 벼 품종의 영과 쌀겨 추출물의 항산화 및 항암활성 변이)

  • Shin, So-Hee;Chung, Nam-Jin
    • Korean Journal of Breeding Science
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    • v.43 no.4
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    • pp.297-303
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    • 2011
  • In order to confirm the biological activities of rice bran and hull, total phenolic content, antioxidant and anticancer activities were examined in three rice varieties that have different seed coat colors such as brown, black, and purple. The antioxidant activity was measured by free radical scavenging assay including DPPH, $ABTS^+$ and reducing power activity. The anticancer activity was evaluated by WST assay with human HepG2 cell lines. As the result, Huegjinju, black seed coat rice, showed higher of the total phenolics contents in both bran and hull, than purple Jeojinju and brown Ilpum. In the antioxidant activity, Heugjinju showed the highest among the bran extracts, but the activities of hull extracts were similar in three varieties. In the anticancer activity to HepG2 cell line, the hull extract was 20% higher than that of bran extract in the average of three varieties. The hull extracts of Ilpum and Jeokjinju showed similar anticancer activites about 92%, and that of Heugjinju showed the lowest activity of 30.4%. The anticancer activities of hull extracts in three varieties showed positive correlation with total phenolics contents, DPPH radical scavenging activities and reducing power activities. In conclusion, the rice bran and hull showed potent antioxidant and anticancer activities varied in three varieties that have different seed coat colors.

Estimation of Environmental Effect and Genetic Parameter on Reproduction Traits for On-farm Test Records (농장검정돈의 번식형질에 미치는 환경효과 및 유전모수의 추정)

  • Jung, D.J.;Kim, B.W.;Roh, S.H.;Kim, H.S.;Moon, W.K.;Kim, H.Y.;Jang, H.G.;Choi, L.S.;Jeon, J.T.;Lee, J.G.
    • Journal of Animal Science and Technology
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    • v.50 no.1
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    • pp.33-44
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    • 2008
  • The purpose of this study was to estimate the genetic parameters and trend of Landrace and Yorkshire pigs, which were raised on private farms from 1999 to 2005 and tested for their reproductive performance by the Korea Animal Improvement Association. Prior to analysis, records without pedigree or having value with larger than±3×standard deviation for the Total number of born were excluded. The effects of breed and environmental factors were estimated with least square method(Harvey, 1979), and estimation of breeding values and genetic parameters were performed on the data of 1’st litter only with GIBBSF90(Misztal, 2001) which was programmed according to Gibbs Sampling method based on Bayesian Inference by Gianola and Fernando(1986), Jensen(1994) and others. Gibbs sampling was performed 50,000 times for each parameter, and the first 5000 samples were regarded as those in burn-in period and thus, excluded for post hoc analysis. Total number of born and total number of accident were statistically significant(p<0.01) for the breed, farrowing year, farrowing season and parity effects, and the number born alive at birth was statistically significantp<(0.01) for the breed, farrowing year, farrowing season and parity effects. No particular trend was observed in the genetic and phenotypic improvement of the total number of born and number born alive at birth before 2001, when the piglet registration system started, but the tendencies of increasing for the total number of born and number born alive and decreasing for the total number of accident were observed since 2001. Somewhat higher heritability estimates of our study seems to be attributed to the situations that first parity records with poor farrowing performances were used in the analyses and it was impossible to obtain accurate reproductive performance due to the absence of criteria for record keeping at the level of individual farms.

Studies on the Inheritance of Agronomic Characteristics in Upland Cotton Varieties (Gossypium hirsutum L.) in Korea (육지면품종의 유용형질의 유전에 관한 연구)

  • Bang-Myung Kae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.21 no.2
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    • pp.281-313
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    • 1976
  • To obtain fundamental informations on cotton breeding efficiences for Korea, individual genetic relationships and interrelationships between the agronomic characteristics of Upland cotton were investigated. These experiments were couducted at the Mokpo Branch Station $(34^{\circ}48'N, $ $126^{\circ}23'E$ and altitude of 10m above sea level) from 1969 through 1972. Heterosis, combining ability, dominance and recessive gene action, genetic variance, and phenotypic and genotypic correlation were investigated by $F_1'S$ from an 11-parent partial diallel cross and the segregating $F_2$ and $F_3$ populations of the cross Paymaster times Heujueusseo Trice. The following points resulted from this study, 1. Heteroses for number of bolls per plant and lint yield were significant at 27, 84% and 37.26%, respectively. No other character had significant heteroses. 2. The GCA estimates for all studied characteristics were higher than the SCA estimates. Varieties with high GCA effects were Suwon 1 for earliness, Paymaster and Arijona for high lint percent, and Arijona for long fiber, etc, 3. SCA estimates for lint yield varied widely in crosses with Mokpo 4, Mokpo 6 and Heujueusseo Trice. Those crosses with the highest SCA effects were combinations with large characteristics differences, Example of these crosses are Mokpo 4 times Acala 1517W, Mokpo 4 times D. P. L. and Heujueusseo Trice aud Paymaster. 4. Early-maturing varieties were completely dominant to late-maturing varieties in some combinations while other crosses gave intermediate phenotypes. These results suggest additive genetic action by multi-genes. Heujueusseo Trice, Mokpo 6, and Suwon 1 showed highest degree of dominance for earliness. 5. There were no significant trends for inheritance of weight of boll and 100 seeds weight. 6. Long staple was partially to completely dominant to short staple. Though there were single gene ratios the rate of dominance decreased in the $F_2$ and $F_3$ populations in the cross between the long staple variety Paymaster and the short staple variety Heujueusseo Trice. Diallel cross $F_1$ hybrids showed complicated allelic gene action for staple length. Various dominance degree were shown by varieties. 7. Number of bolls per plant indicated strong over-dominance and small non-allelic additive gene action. 8. Lint Yield was characterized by over-dominance and by multiple non-allelic-gene action. High-yielding varieties were dominant to low-yielding ones. However, the low-yielding variety Heujueusseo Trice showed over-dominance, indicating different reactions according to the varieties and combinations. 9. Broad sense heritability for days to flowering was 34-39% while narrow sense heritability was 11%. Large variations of individual plants caused by Korean climatic conditions cause this situation. Heritability estimates for weight of boll was 30% for broad sense and 22% for narrow sense. 10. Heritability estimates for staple length and lint percent were very high suggesting strong selection effects. 11. Narrow sense heritability estimates for number of bolls per plant was 30% in the diallel cross $F_1$ hybrids and 36% in the $F_2$ population of the special cross. Broad sense heritability was estimated at 67% suggesting that. 12. Heritability estimates for lint yield was low due to high over-dominance in the diallel cross $F_1$ hybrids. Heritability estimates for yield was low in the $F_1$ hybrids but high in the $F_2$ and $F_3$ populations. 13. Phenotypic and genotypic correlations between lint percent and days to flowering and between staple length and days to flowering were high in the $F_1, $ $F_2$ and $F_3$ populations. Late-maturing varieties and individuals had long staple and high lint percent in general. As the correlation between days to flowering and lint yield was extremely low, the two traits were considered independent of each other. Days to flowering and number of bolls per plant were negatively correlated in the $F_3$ population, indicating early-maturing individual plants with many bolls may be readily selected. 14. Phenotypic and genotypic correlations between lint percent and staple length were high in $F_1, $ $F_2$ and $F_3$ populations. Accordingly, long staple varieties were high in lint percent. It was recognized that lint yield and lint percent were positively correlated in the diallel cross $F_1$ hybrids, and lint percent and staple length were positively correlated in the $F_2$ population, indicating that lint percent and staple length affect lint yield. 15. Lint yield was significantly and positively phenotypically correlated with number of bolls per plant in $F_1, $ $F_2$ and $F_3$ populations. A high genotypic correlation was also noted indicating a close genetic relationship. The selection efficiencies for a high-yielding variety can be increased when individual plants with many bolls are selected in later generations. The selection efficiencies for good fiber quality can be enhanced when individuals with long staple and high lint percent are selected in early generations.

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Studies on Growth Performance and Meat Quality Improvement of the Unselected Hanwoo Bulls in the Performance Test (한우 당대검정 탈락축의 산육능력 및 육질 향상에 관한 연구)

  • Kim, Hyeong-Cheol;Lee, Chang-Woo;Park, Byung-Ki;Lee, Sang-Min;Kwon, Eung-Gi;Im, Seok-Ki;Jeon, Gi-Jun;Park, Yeon-Soo;Hong, Seong-Koo
    • Journal of Animal Science and Technology
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    • v.52 no.5
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    • pp.427-434
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    • 2010
  • This study was conducted to investigate the growth performance and meat quality improvement according to castration, optimal feeding management and ruminally protected amino acid-enriched fatty acid (RPAAFA) for the unselected Hanwoo bulls in the performance test. Bulls were castrated at approximately 14 months of age. Sixteen Hanwoo steers, 15 months of age and weighing $412.9{\pm}24.9kg$, were distributed into 2 groups. Steers were fed a basal diet supplemented with RPAAFA at 0 g (control) or 100 g (treatment), respectively for 12 months. Steers were slaughtered at 27 months of age. Average daily gain for treatment tended to be higher (p=0.10) than that of control, whereas feed conversion ratio tended to be lower (p=0.07) in treatment than in control. The supplementation of RPAAFA did not affect rib eye area, back fat thickness, meat color, fat color, texture and maturity. The appearance rates of yield 'A' grade and high quality grade ($1^{++}$, $1^+$ and 1) were higher in treatment than in control. The content of moisture, fat, protein and ash in longissimus muscles were similar between control and treatment. The supplementation of RPAAFA did not affect water-holding capacity, oxidation and reduction potential, myoglobin and fatty acid contents in longissimus muscles. Thus, present results indicate that castration, optimal feeding management and RPAAFA may be recommended for improving growth performance and quality grade of the unselected Hanwoo bulls in the performance test.

The Factors Influencing the Asthenopia of Emmetropia with Phoria (사위를 가진 정시안의 안정피로에 영향을 미치는 요인)

  • Kim, Jung-Hee;Lee, Dong-Hee
    • Journal of Korean Ophthalmic Optics Society
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    • v.10 no.1
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    • pp.71-82
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    • 2005
  • The aim of this study was to provide fundamental data for the factors influencing the asthenopia of emmetropia with phoria and alleviation of asthenopia. A total of 348 subjects, aged between 19 and 30 years old, who had no strabismus, an eye trouble or whole body disease, were examined using corrected visual acuity, corrected diopter, stereopsis and suppression tests from September of 2002 to September of 2004. We excluded 21 subjects for the following reasons: if they had an amblyopia affecting binocular vision or inaccurate data. After these exclusions, 327 subjects remained. We then individually measured the refractive error correction, pupillary distance, optical center distance, phoria, convergence, accommodation and the AC/A as well as the asthenopia during binocular vision using a questionnaire. After analysis of factors affecting asthenopia, we also examined the reductive effect of a prism on the asthenopia in subjects who had asthenopia. To determine the factors affecting asthenopia during binocular vision, statistic analyses were carried out using the Chi-square test and the multivariate Logistic regression model. The results of this study were as follow. For asthenopia during near binocular vision of emmetropia with phoria, in case of the lower the accommodation and convergence, a significantly higher rate of asthenopia was observed (p<0.001). When the AC/A is lower, the higher the rate of asthenopia was observed but not significantly and there was no association between phoria and asthenopia. When the multivariate logistic regression model was used to determine factors affecting binocular vision of emmetropia with phoria, in case of the lower accommodation and convergence, a significantly higher rate of asthenopia was observed. when the phoria is esophoria or higher exophoria, or when the AC/A is lower than normal, the higher the rate of asthenopia was observed but not significantly and there was no association between phoria. AC/A and asthenopia. Therefore accommodation and convergence could be predictive factors for asthenopia during near distance binocular vision. Prism was used among' subjects who had asthenopia during near distance binocular vision, the symptom of asthenopia was eased up to 74.2% in emmetropia with phoria.

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COMORBIDITY AND RISK FACTORS ASSOCIATED WITH CHILDREN WHO HAVE THE SYMPTOMS OF OPPOSITIONAL DEFIANT DISORDER - COMMUNITY BASED STUDY - (반항성 도전 장애 아동과 연관된 공존 증상 및 위험 요인에 관한 연구 - 지역사회 연구 -)

  • Kim Boong-Nyun;Jung Kwang-Mo;Cho Soo Churl;Hong Kang-E
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.16 no.1
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    • pp.79-89
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    • 2005
  • Objectives : To acquire an improved understanding of oppositional defiant disorder, we evaluated the characteristics of children who have the symptoms of ODD in community sample. Methods : 1200 children from an elementary school in Bucheon (an urban community near Seoul) were recruited by randomized sampling method. By Disruptive Behavior Disorder Scale according to DSM-III-R & DSM-IV, we evaluated the symptoms of ODD and selected subjects with ODD. Psychiatric comorbidity, character trait were compared in subjects with ODD and comparison group. Also we examined the association between prenatal/perinatal risk factors, family functions and the symptoms of ODD. Data were analyzed by appropriate statistical method using SPSS 11.5 window version. Result : Children with oppositional defiant disorder were revealed to have significantly higher rates of psychiatric comorbidity and significantly greater family dysfunction compared to comparison group. Among the prenatal/perinatal risk factors, severe emotional stress during pregnancy, postpartum depression, medication during pregnancy were revealed as risk factors of ODD. In character inventory, ODD group were evaluated to have high score in novelty seeking, harm avoidance, but low in reward dependency. Conclusion : These results support that 1) prenatal/perinatal and psycho-social risk factors could be a important role in the progression of ODD, and 2) children with ODD have diverse comorbid psychiatric symptoms.

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