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The Effect of Water Temperature on Egg Developmental Stages of Largescale Blackfish Girella punctata and Smallscale Blackfish Girella melanichthys (벵에돔 Girella punctata와 긴꼬리 벵에돔 Girella melanichthys의 난 발생에 미치는 수온의 영향)

  • Oh, Bong-Se;Choi, Young-Ung;Ku, Hag-Dong;Kim, Sung-Chul;Jung, Min-Min;Park, Heung-Sik
    • Development and Reproduction
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    • v.14 no.2
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    • pp.51-58
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    • 2010
  • This research investigated that the effect of water temperature on egg developmental stages of largescale blackfish Girella punctata and smallscale blackfish Girella melanichthys. The required times from fertilized embryos to hatching for the G. punctata were 67.8~37.5 hrs from 15 to $21^{\circ}C$, shorter as the water temperature increased. But fertilized embryos were dead after Kuffer's vesicle appearance stage at $24^{\circ}C$ and morula stage at $27^{\circ}C$. Biological minimum temperature (starting point for embryonic development) of the egg development was estimated to be $7.6^{\circ}C$ in average. In G. melanichthys, fertilized embryos were hatched within 61.2~38.3 hr from 15 to $21^{\circ}C$, the times were shorter as the water temperature increased to hatching, but its were dead on above $24^{\circ}C$. Biological minimum temperature of the egg development was estimated to be $6.5^{\circ}C$ in average. Based upon these results, it is recommended that the range of optimum water temperature for embryonic development of G. punctata and G. melanichthys is $15{\sim}21^{\circ}C$.

Change Prediction for Potential Habitats of Warm-temperate Evergreen Broad-leaved Trees in Korea by Climate Change (기후변화에 따른 한반도 난온대 상록활엽수의 잠재 생육지 변화 예측)

  • Yun, Jong-Hak;Nakao, Katsuhiro;Park, Chan-Ho;Lee, Byoung-Yoon;Oh, Kyoung-Hee
    • Korean Journal of Environment and Ecology
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    • v.25 no.4
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    • pp.590-600
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    • 2011
  • The research was carried out for prediction of the potential habitats of warm-temperate evergreen broad-leaved trees under the current climate(1961~1990) and three climate change scenario(2081~2100) (CCCMA-A2, CSIRO-A2 and HADCM3-A2) using classification tree(CT) model. Presence/absence records of warm-temperate evergreen broad-leaved trees were extracted from actual distribution data as response variables, and four climatic variables (warmth index, WI; minimum temperature of the coldest month, TMC; summer precipitation, PRS; and winter precipitation, PRW) were used as predictor variables. Potential habitats(PH) was predicted 28,230$km^2$ under the current climate and 77,140~89,285$km^2$ under the three climate change scenarios. The PH masked by land use(PHLU) was predicted 8,274$km^2$ and the proportion of PHLU within PH was 29.3% under the current climate. The PH masked by land use(PHLU) was predicted 35,177~45,170$km^2$ and increased 26.9~36.9% under the three climate change scenarios. The expansion of warm-temperate evergreen broad-leaved trees by climate change progressed habitat fragmentation by restriction of land use. The habitats increase of warm-temperate evergreen broad-leaved trees had been expected competitive with warm-temperate deciduous broadleaf forest and suggested the expand and northward shift of warm-temperate evergreen broad-leaved forest zone.

Artificial Activation of Pig Oocytes Arrested at meiotic Metaphase II (제2감수분열 중기에서 발달정지된 돼지 난자의 인위적 난활성)

  • R. S. Prather;;Z. Mach ty
    • Korean Journal of Animal Reproduction
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    • v.20 no.4
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    • pp.395-412
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    • 1997
  • Mammalian eggs are ovulated arrested at meiotic metaphase II until fertilization. Generally in mammals, fertilization results in a series of intracellular calcium oscillations that are mediated by inositol triphosphate (IP$^3$) or cyclic adenosine diphosphoribose (cADPr). The high levels of maturation promotion factor (MPF) within the cell decrease, pronuclei form, the cytoskeleton is reorganized and proteins are post-translationally modified. If all is normal, the newly formed embryo initiates the developmental program specific to that species. Artificial methods of producing these effects in pig oocytes are discussed. One potential mechanism mediated via a signal transduction pathway is present in pig oocytes. Stimulation of this pathway leads to the early events following fertilization, and electrical stimulation leads to apparently normal de v velopment to day 12. Further studies are needed to determine which mechanism(s) the sperm uses to initiate development.

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Spatio-temporal distribution of the Ichthyoplankton in the Mankyong-Dongjin Estuary (만경 동진강 하구의 浮流性 卵 仔稚漁 分布 樣相)

  • 차성식;박광재
    • 한국해양학회지
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    • v.26 no.1
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    • pp.47-58
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    • 1991
  • To study the spatio-temperal distribution of the ichhyoplankton in the estuarine ecosystem, Ichthyoplankton were sampled with associated data on water temperature and salinity in Mankyong-Dongjin Estuary on October, 1989, and March, May and July, 1990. Water temperature varied seasonally, showing homogeneous spatial pattern. The spatial difference in splinty was determined by the amount of river runoff. Few Ichthyoplankton occurred in October and March. In May, Konosirus punctatus occupied most of the ichthyoplankton. In July, Sardinella zunasi, Engraulis japonica, Gobiidae, and Thrissa sp. were the dominant species. The eggs and larvae of Engraulis japonica were collected from the outer bay, while those of Konosirus punctatus predominated in the less saline inner bay. Konosirus punctatus. Sardinella zunasi, Gobiidae, and Thrissa sp. were the characteristic species of this estuary. They were considered to be spawned and grown during their early life stage in the less saline water.

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Distribution of the Ichthyoplankton in Kwangyang Bay (광양만 부유성 난 자치어의 분포)

  • Cha, Seong-Sig;Park, Kwang-Jae
    • Korean Journal of Ichthyology
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    • v.6 no.1
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    • pp.60-70
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    • 1994
  • To study the composition and distribution of the ichthyoplankton in Kwangyang Bay, they were sampled at eight stations from February to December 1990, Abundance was the highest in June, and the number of taxa was the highest in August. Engraulis japonicus, Leiognathus nuchalis, Konosirus punctatus and Argyrosomus argentatus were dominant among 6 taxa of pelagic eggs. Engraulis japonicus, Gobiidae, Korwsirus punctatus, Argyrosomus argentatus and Leiognathus nuchalis were dominant among 21 taxa of fish larvae. The number of taxa was smaller than the previous study. Abundance during winter was low due to the reduction of abundance of winter spawning species. Ammodytes personatus and Enendrias fangi.

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Oviposition Behavior of Ooencyrtus kuvanae (Howard) (Hymenoptera : Encyrtidae), Egg Parasitoid of Lymantria dispar L. (Lepidoptera : Lymantriidae) (매미나방알좀벌, Ooencyrtus kuvanae (Hymenoptera : Encyrtidae)의 매미나방 난에 대한 산란행동)

  • 이해풍;이장훈
    • Korean journal of applied entomology
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    • v.28 no.4
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    • pp.221-228
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    • 1989
  • The oviposition behavior of Ooencyrtus kuvanae (Howard) (Hymenoptera: Encyridae) was examined in a small petri dish containing eggs of gypsy moth, Lymantrt'a dt'spar L. The behavioral sequence from initial searching movement toward the host egg through oviposition, departure, and auxiliary phase activities such as resting, glooming, and host feeding were described. Oviposition experience shortened the approach time to subsequent eggs. The time spent for contacting the host egg also decreased with oviposition experience. Oviposition experience may play an important role in host recognition and increasing oviposition efficiency.

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Comparison of Carotenoid Pigments in Flat bitterling, Paracheilognathus rhombea and Korean striped bitterling, Acheilognathus yamatsutae Mori, in the Subfamily Cyprinidae (잉어아과에 속하는 납지리와 줄납자루의 Carotenoid 색소성분의 비교)

  • 권문정;하봉석
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.1
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    • pp.80-86
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    • 1996
  • 어류의 carotenoid에 관한 비교 생화학적 연구의 일환으로서 잉어아과에 속하는 납지리와 줄납자루의 표피와 난의 carotenoid 조성을 비교 검토하여 다음과 같은 결론을 얻었다. 납지리와 줄납자루 표피의 총 carotenoid 함량은 납지리 수컷 2.3mg%, 납지리 암컷 1.1mg%, 줄납자루 수컷 1.9mg%, 줄납자루 암컷 0.8mg%로 나타나 수컷 표피의 총 carotenoid 함량이 암컷 표피보다 다소 높았다. 표피의 carotenoid 조성은 lutein, lutein epoxide, tunaxanthin, zeaxanthin, triol 이 주성분을 이루었고 그외, $\beta-carotene,$ $\alpha-cryptoxantinm$ cyn-thiaxantin 이 함유되어 있었다. 납지리 수컷의 표피에는 줄납자루 수컷에 비해 lutein의 함량치가 높았고 줄납자루 수컷의 경우는 납지리 수컷이 비해 lutein epoxide와 tunaxanthin의 함량치가 높았다. 그리고 납지리 암컷을 표피에는 줄납자루 dkat에 비해 lutein epoxide 함량치가 높았도 줄납자루 암컷의 표피에는 납자리 와 줄납자루 난의 총 carotenoid 함량은 납지리 1.8mg%, 와 줄납자루 2.5mg%로 표피와는 달리 줄납자루 난의 함량치가 높았다. 납지리와 줄납자루 난의 carotenoid 조성은 다 같이 lutein, lutein epoxid 가 주성분을 이루었고 그외 triol은 표피에 비해 적게 함유되어 있었다. 동일한 잉어아과의 납지리와 줄납자루의 표피 및 나의 carotenoid 조성은 서로 유사함을 확인 할 수 있었다.

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Parametric Study of a Diode Side-Pumped Nd:YAG Laser Using a Diffusive Reflector (난반사체를 이용한 다이오드 횡여기 Nd:YAG 레이저의 매개변수 연구)

  • 이성만;김선국;윤미정;문희종;김현수;고도경;차병헌;이종민
    • Proceedings of the Optical Society of Korea Conference
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    • 2000.08a
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    • pp.222-223
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    • 2000
  • 지금까지 gold-coated 반사체, cusp-shaped 반사체, 복합 포물 반사체(CPC), 난반사 공동체 등 다양한 형태의 반사체가 횡펌핑을 이용한 Nd:YAG 레이저의 개발을 위하여 고안되어졌다. 횡여기되는 Nd:YAG 레이저의 광학적 효율은 반사체의 형태, 다이오드 레이저의 파장, Nd:YAG 결정의 지름, Nd$^3$의 도핑농도에 따라 영향을 받는다.$^{(1)}$ 본 연구에서는 고출력의 횡여기 Nd:YAG 레이저를 개발할 목적으로 광여기 공동으로 사용된 난반사 공동체의 내부지름과 레이저 매질인 Nd:YAG 결정의 흡수계수 등 매개변수를 변화시켜 흡수분포와 출력을 계산하였으며, 이러한 매개변수들이 기울기 효율에 미치는 영향을 수치 해석적으로 연구해 보았다. 광선추적법에서 사용한 매개변수 중에서 다이오드 레이저의 파장은 808 nm이고, 반사율은 90%이며, 여기 다이오드 출력은 1080 W이다. 난반사 공동체$^{(2)}$ 의 반사율은 95.7%이고, 결정의 투과율은 90%이며, 결정의 반지름은 2.5 mm이다. 사용된 여기 구조도는 그림 1과 같다. 여기헤드는 Nd:YAG 결정, 난반사 공동체, 그리고 3개의 360 W급 다이오드 어레이들로 구성되어 있다. (중략)

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대농갱이 인공종묘생산

  • 이춘희;정만복;김중환;이정열
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.51-51
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    • 2003
  • 대농갱이는 체형이 동자개와 비슷하나 지금까지 인공부화는 물론 산란습성에 관해서도 잘 알려져 있지 않은 미개발 어종으로 양식품종의 다양화 및 경쟁력 있는 새로운 품종 개발을 위하여 인공종묘생산 시험을 시도하였다. 본 연구에 사용한 대농갱이 친어는 금강 중류역에서 서식하고 있는 자연산 친어로 암컷 210마리, 수컷 148마리를 사용하였다. 호르몬주사에 의한 인공 배란효과 조사를 위해 암컷 한 마리당 HCG 1,000 IU 주사군(75마리)과 2,000 IU 주사군(135마리)으로 나누어 배란율을 조사하였으며, 수컷은 주사하지 않고 정소를 적출하여 막자사발에 갈아서 등조액과 혼합하여 사용했다. 수정 방법은 난을 착출하면서 정자 등조액을 동시에 흘리는 방법, 난을 그릇에 받은 후 정자 등조액을 혼합 수정하는 방법, 채란용기에 정자 등조액을 미리 넣고 암컷의 난을 짜 넣어 수정시키는 방법 등 3가지 방법을 사용하였다. 부화된 자어는 초기먹이로 알테미아(3일 급이), 실지렁이(6일 급이), 배합사료 순으로 급이하였으며 수온 25~28$^{\circ}C$에 유지관리하면서 성장도 시험을 실시하였다. 호르몬 주사에 의한 배란효과는 HCG 2,000 IU 주사군(채란율 88.9%)이 1,000 IU 주사군(37.3%)에 비해 월등히 높았으며 난의 성숙상태도 좋았다. 수정방법에 있어서는 채란과 동시에 정자 등조액을 흘려 수정시키는 방법과 채란용기에 미리 정자 등조액을 넣고 암컷의 난을 한 마리씩 짜서 수정한 후 바로 부화지로 이동하여 고르게 뿌려 준 경우가 수정율 및 부화율이 좋았다. 부화수온은 23$\pm$1$^{\circ}C$에서 96시간, 26$\pm$1$^{\circ}C$에서는 72시간만에 부화되었으며 부화여건이 좋으면 단시간에 부화할 수 있도록 하는 것이 수생균 발생을 억제할 수 있어서 효과적이었다. 자치어의 성장도 실험에서는 사육수온 25~28$^{\circ}C$에서 직선식으로 나타낼 수 있어서 평균체장은 Y=0.9943X+0.7867, 평균체중은 Y=0.8800-0.6800로 각각 표시되었다.

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Distribution of Ichthyoplankton in the Adjacent Waters of Wolsong, Korea (월성 주변 해역의 부유성(浮游性) 난(卵)과 자치어(仔稚魚)의 분포(分布))

  • Cha, Seong-Sig;Park, Kwang-Jae;Yoo, Jae-Myung;Kim, Yong-Uk
    • Korean Journal of Ichthyology
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    • v.3 no.1
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    • pp.11-23
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    • 1991
  • To study the distribution of inhthyoplankton in the adjacent water of Wolsong, samples were collected using Bongo net from the surface water in November of 1989, and February, May and July of 1990. Fish eggs were divided into Engrualis japonicus, Callionymidae, Maurolicus muelleri, Konosirus punctatus, and others. The dominant species of fish eggs were Maurolicus muelleri and Callionymidae accounting for 60.1% and 13.7% of the total egg abundance, respectively. Total of 21 taxa of fish larvae occurred. The predominant species was Engraulis japonicas accounting for 61.2% of the total lavae, and bollowed by Callionymidae(11.9%). Other major species were Maurolicus muelleri, Gobiidae sp., Ammodytes personatus, Sebastes inermis, S. Pachycephalus, Scartella cristata, and Sebastiscus marmoratus. These 9 taxa constituted 96.5% of the total larvae collected. In this area, where upwelling occasionally occurs the distribution of fish eggs and larvae appeared to be affected by the transport of the surface water during the upwelling period.

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