• Journal of the Korean Society of Tobacco Science
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• v.19 no.2
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• pp.117-123
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• 1997

Viral coat protein (CP) encoding cDNA with artificial start and stop codons was synthesized by reverse-transcriptase polymerase chain reaction (RT-PCR) from the Korean isolate of potato virus Y-vein nectrosis strain (pVY-VN). To make PVY CP cDNA to untranslatable form, three stop codons were inserted near the start codon by "megaprimer-PCR" method. The untranslatable CP cDNA was subcloned to plant expression vector and transferred to N. tabacum cv. NC82 by Agrobacterium-mediated transformation. Highly resistant plants to PVY infection were screened, based on symptom development after mechanical virus inoculation. By genomic PCR and Southern blot analysis, one or more copies of the untranslatable CP gene were found in all transformants. From northern blot analysis, highly resistant transgenic lines had very low level of CP transcript but susceptible lines had high level, suggesting resistance to PVY infection should be related to RNA-mediated mechanism.mechanism.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.1881-1884
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• 2003

Diamond turning machines have, been used for the processing of surface like a mirror with the control scheme of minimizing shape error, Ultra-precision diamond fuming is applied to produce highly precision optical components required not only a high machining accuracy but also a good surface roughness. Al-6061 is widely used as optical parts such as laser reflector's mirror or multimedia instrument. In this study, thermal-imaged Al flat mirrors are fabricated by SPDT. The surface roughness 3.472 nm Ra, power 2 fringe(at 632.8 nm) and irregularity 1 fringe(at 632.8 nm) for form waviness of thermal-imaged Al flat mirror are very satisfied to the required specification in industry.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.525-528
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• 2003

The aspherical lens are used as objective lens of optical pickup. The sample product is made before manufacturing the injection mould of lens to examine the design factor. The optimum cutting conditions of the main spindle speed, the depth of cut, the feed rate are found when we cut PMMA and PC lens sample with ultra-precision SPDT. The demanded surface roughness 10 nm Ra. aspherical form error 0.5 ${\mu}{\textrm}{m}$ P-V for aspherical lens of optical data storage device are satisfied for PMMA. but not satisfied for PC.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2000.11a
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• pp.68-71
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• 2000

A tool holder system has been designed to measure cutting forces in diamond turning. This system includes a 3-component piezo-electric tranducer. In this research, tool holder system is modeled by considering the element dividing, material properties, and boundary conditions using MSC/PATRAN. Mode and frequency analysis of structure is simulated by MSC/NASTRAN, for the purpose of developing the effective design. In addition, tool holder system is verified by vibration test using accelerometer. This system will aid to the development of Fast Tool Servo.

• Korean Journal Plant Pathology
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• v.13 no.1
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• pp.63-68
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• 1997

In the screening of antiviral materials produced by actinomycetes, an isolate named B25 was fond to produce an antibiotic substance ASA, which showed a strong inhibitory activity against tobacco mosaic virus (TMV) infection. ASA was purified from culture broth of B25 by silica gel column chromatography, preparative TLC, and reversed phase HPLC. Also MS, IR, UV spectrum, and melting point of ASA were determined and analysed. ASA was white powder soluble in dimethyl sulfoxide, chloroform, and ethyl acetate, having absorption peaks at 223 and 328 nm in UV-VIS spectrum, and had a molecular weight of 548. ASA showed strong inhibitory effect on TMV infection when it was applied as a mixture of TMV to the upper surface of leaves of a local lesion host (Nicotiana tabacum c. Xanthi-nc). It also showed antimicrobial effect against yeast and some phytopathogenic fungi.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2000.05a
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• pp.591-594
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• 2000

In the recent day, fatigue life prediction techniques play a major role in the design of components in the ground vehicle industry. Full scale durability testing in the laboratory is an essential of any fatigue life evaluation of components or structure of the automotive vehicle. Component testing is particulary important in today's highly competitive industries where the design to reduce weight and production costs must be balanced with the necessity to avoid expensive service failure. Generally, 3-axis durability testing device is used to carry out the fatigue test. In this paper, The operation software for simultaneously driving 3-axis vibration testing device is developed and the displacement of the 3-axis actuator is separately calibrated by LDT Moreover, the input and output data are displayed in windows of PC controller with real time.

• 제어로봇시스템학회:학술대회논문집
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• 2000.10a
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• pp.556-556
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• 2000

In the multi-purpose lathe, the design of tilting turret slide system has an important and critical role to enhance the accuracy of the machining process. Tilting turret unit is traveled by 3-axis slide systems. There is a need to design this part very carefully. In this research, the 3-axis slide system with tilting turret unit is researched with two approaches; The first is that 3-axis slide system is modeled and simulated using ADAMS software. The dynamic behavior of this system is visualized by data graphs and dynamic animations. The second is that the slide system is analyzed with the aspect of stress distribution. The slide system is modeled and displayed by PATRAN and analyzed by NASTRAN. The analysis of strain and stress distribution in the each node is prompted and visualized in the computer. The first step of virtual prototype which makes it possible to design economically and effectively is developed.

• 제어로봇시스템학회:학술대회논문집
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• 2000.10a
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• pp.141-141
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• 2000

In the recent day, fatigue life prediction techniques play a major role in the design of components in th ground vehicle industry. Full scale durability testing in the laboratory is an essential of any fatigue life evaluation of components or structure of the automotive vehicle. Component testing is particularly important in today's highly competitive industries where the design to reduce weight and production costs must be balanced with the necessity to avoid expensive service failure. Generally, multi-axis durability testing simulator is used to car교 out the fatigue test. In this paper, the operation software for simultaneously driving 3-axis simulator is developed and the real-time signals of input-output data are displayed in window of PC. Moreover, the displacements and the loads of 3-axis actuators are calibrated separately and the operating characteristics of the actuators are evaluated.

• 제어로봇시스템학회:학술대회논문집
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• 2000.10a
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• pp.208-208
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• 2000

In realistic control systems, the nonlinear salutation attributes of the control actuator due to physical limitations should be taken into account. This nonlinear saturation of actuators may cause not only deterioration of the control performance but also a large overshoot during start-up and shut-down. As the overshoot increases, the system may become oscillatory unstable. In this paper, the supervisor implementation which guarantees good performance lot saturation operation and prevents reset wind-up is presented. Moreover, the sufficient conditions of the stability for saturated systems using supervisory control with a dynamic controller are provided in the discrete-time domain. A numerical example is illustrated to depict the efficiency of supervisory control for a typical saturated production-distribution system controlled by a discrete-time dynamic controller and to validate basic results by simulation.

• Korean Journal Plant Pathology
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• v.12 no.1
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• pp.80-84
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• 1996

토양에서 분리한 방선균류(actinomycetes) GT103 균주의 배양여액 및 균체추출물은 담배의 주요 병원균인 담배 줄기속마름병균(Erwinia carotovora subsp. carotovora), 담배 탄저병균(Colletotrichum tabacum), 담배역병균(Phytophthora nicotianae var. nicotianae)등에 강한 항균활성을 보였다. 항균활성물질은 용매 추출, silica gel chromatography, HPLC 등을 실시하여 분리.정제하였으며 UV, IR, FAB-MS, \ulcornerH-NMR, \ulcornerC-NMR 분석결과 actinomycin group의 펩타이드계 항생 물질인 actinomycin C\ulcorner로 동정되었다. 이 활성물질의 담배 탄저병에 대한 방제효과는 50$\mu\textrm{g}$/ml 농도에서 100%, 10$\mu\textrm{g}$/ml 농도에서 90%의 방제효과를 보였다.