• Title/Summary/Keyword: 기초대사

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Suppression of Lipid Peroxidation and CYP Isozymes activities by Circium japonicum Herbal-acupuncture Solution ; Basic Study for Screening of Medicinal Herb on Reactive Oxygen Radical and CYP-Mediated Atherosclerosis (대계 약침액(藥鍼液)의 지질과산화 및 CYP 억제에 미치는 영향(影響) ; 활성산소자유기 및 CYP 매개의 동맥경화 치료를 위한 천연약물 개발의 기초 평가)

  • Lee Jeong-Joo;Kim Hyuck;Yi Hyo-Seung;Park Won-Hwan;Moon Jin-Young
    • Korean Journal of Acupuncture
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    • v.23 no.4
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    • pp.177-186
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    • 2006
  • 목적 : 약침액(藥鐵液)의 지질과산화 예방 및 cytocome P450과의 상호 작용에 있어서 대계의 역할은 과거 연구가 거의 없었다. 따라서 본 실험에서는 대계 약침액이 지질과산화를 예방하고, 심혈관계질환 유발에 밀접한 연관이 있는 cytochrome P450의 직접적인 저해 효과를 검토 하고자 한다. 방법 : 대계 약침액이 지질과산화를 억제하는 정도를 평가하기 위하여 세포막을 구성하는 불포화지방산의 일종인 linoleic acid를 대상으로 지질과산화 진행 시간과 대계 약침액의 농도에 의존적인 저해 효과를 실험하였다. 또한 실험쥐의 간조직을 이용하여, 강제적인 과산화를 유도한 후 이를 방어하는 효능을 검토하였다. 그리고 cytochrome P450을 구성하는 그룹의 1A1, 1A2 및 2E1의 활성을 각각 EROD, MROD, p-nitrophenol, aniline 방법으로 측정하였다. 결과 및 결론 : 대계 약침액은 세포막 구성의 불포화 지방산인 linoleic acid의 산화를 시간 및 처리 농도에 의존적으로 억제하였고, 실험쥐의 조직 과산화를 유의성 있게 저해하였다. 또한 aryl hydrocarbon receptor (AHR)을 활성화 시켜 polycyclic aromatic hydrocarbons (PAHs)에 의한 심혈관계 질환 유발 인자로 알려진 cytochrome P450 1A1 및 1A2의 발현을 일부 저해하였으며, 특히 체내에 흡수된 알콜 대사에 관여하는 P450 2E1을 강하게 억제 시켰다.

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Changes of Sugar Content during Germination and Germinability in Corn (옥수수 발아시 종자내 당함량의 변화와 발아 특성)

  • 김종진;이영찬
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.6
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    • pp.768-774
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    • 1995
  • These experiments were conducted to determine the change of sugar content during germination in corn, which were analyzed by using HPLC. Germination percentage and growth rate of Golden cross bantam 70 were higher than those of Suweon 19. The emergences of radicle and plumule of Golden cross bantam 70 were faster compared to those of Suweon 19. Three major components, sucrose, glucose and fructose, were detected during germination. Content of sucrose in two tested hybrids decreased rapidly as time passes. In embryo of Suweon 19, the content of sucrose was 38.92% on 12 hours after incubation but decreased to 4.52% on 72 hours. In that of Golden cross bantam 70, it decreased rapidly more than Suweon 19 from 53.03% on 12 hours to 8.18% on 72 hours. On the other hand, the contents of glucose and fructose increased.

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Forest Vegetation of Upper Zone in Gyeryongsan National Park (계룡산 상부 지역의 산림식생)

  • 김효정;이미정;이규석;박관수;송호경
    • Korean Journal of Environmental Biology
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    • v.22 no.1
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    • pp.127-132
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    • 2004
  • The purpose of this study was to understand plant community structure in upper zone of Gyeryongsan National Payk. Total 51 plots were set up and surveyed toy this study. The fifty one plots were classified into four communities such as Quereus mongolica community, Carpinus laxiflora community, Pinus densiflora community, and Quercus variabizis community. The Quercus mongotica community were found in 29 plots of the 51 plots and the differential species in the community weve Quercus mongolica, Acer pseudosieboldianum var. koreanum, Arisaema amurense var. serratum, Diarrhena japonica, and Saussurea gyacilis. The Carpinus laxiflora community were found in 10 plots of the 51 plots and the differential species in the community were Carpinus laxiflora, Carpinus cordata, Cornus eontroversa, Carex siderosticta, and Hydrangea serrata for. acuminata. The Pinus densiflora community were found in 7 plots of the 51 plots and the differential species in the community were Pinus densiflora, Lespedeza maximowiczii, Rubus crataegifotius, Miseanthus sinensis, Persicaria filiforme, Artemisia keiskeana, and spodiopogon sibiricus. the Quereus vuriabilis community were found in 5 plots of the 51 plots and the differential species in the community were Quercus variabilis.

Effect of flexi-bar exercise on body composition and trunk muscle strength in overweight and obesity female college students (플렉시-바 운동이 과체중 및 비만 여대생의 신체조성과 몸통근력에 미치는 효과)

  • Um, Ki-Mai;Wang, Joong-San
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.10
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    • pp.6543-6550
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    • 2015
  • This study aimed to analyze the effect of flexi-bar exercise on in body composition and trunk muscle strength of female college students who are either overweight or obesity. The subjects were female college students divided into experimental group(N=15) and control group(N=15). The experimental group performed a flexi-bar exercise for of 30 minute sessions three days per week for five weeks, and the differences in body composition and trunk muscle strength before and after the experiment and between experimental group and control group were analyzed. The study results showed that the experimental group had a statistically significant improvement in the body fat mass and body fat percentage, body muscle mass, both arm muscle mass, trunk muscle mass, basal metabolic rate(p<.05). Changes in trunk muscle strength showed that the experimental group had statistically significant increased in changes in trunk muscle strength(p<.01). The study verified that flexi-bar exercise can be effective for improving the body composition and trunk muscle strength of overweight and obesity female college students.

Characterization of Polyphosphate Kinase Gene in Serratia marcescens (Serratia marcescens의 Polyphosphate Kinase 유전자 특성)

  • Yang Lark Choi;Seung Jin Lee;Ok Ryul Song;Soo Yeol Chung;Young Choon Lee
    • Journal of Life Science
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    • v.10 no.4
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    • pp.397-402
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    • 2000
  • Polyphosphate kinase catalyzes the formation of polyphosphate from ATP. To understand the mechanism of phosphate accumulation, the Serratia marcescens gene encoding ppk was cloned from the genomic library by the method of Southern hybridization. The hybridization positive DNA fragment region from pDH3 was subcloned into the expression vector. The ppk gene product, a polypeptide of 75 kDa, was confirmed by SDS-PAGE. Expression of the Serratia marcescens ppk is regulated by the catabolite repression system. The enzyme activity polyphosphate kinase was increased in the E. coli strain harboring plasmid pMH4 with ppk gene.

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The Remedial Effect Measurement of an Obesity Remedy Machine for Home Use (새로운 가정용 비만치료기의 비만치료효과 측정)

  • Lee Jae-Hoon;Lee Dong-Hyung
    • Science of Emotion and Sensibility
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    • v.8 no.1
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    • pp.37-45
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    • 2005
  • This paper reports the remedial effect measurement of an obesity remedy machine for home use which has been developed by H Co. and the authors. It is expected that the machine enhances it's remedial effect and usability by utilizing medium frequency and thermotherapy belt etc. In order to measure it's remedial effect, a clinical experiment, which participates eight young female subjects, has been conducted for one month. The experiment includes the measurements on the changes of Gas-Exchange Responses of subjects through Cardio-Pulmonary Exercise Testing. The experimental results show that the obesity remedy machine helps the subjects to reduce their weights, fat rates, and $VCO_2s$. Thus, it turns out that the machine can be a good candidate for medical treatment on the obesity.

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Analysis of Metabolite and Sensory Evaluation on Kunrak, for Reproduced Manufacturing from Old Literature of 「Imwonsibyukji」 (「임원십육지」의 조리법에 기초하여 재현한 건락의 대사체 분석과 관능평가)

  • Jung, Jin-Kyoung;Park, Sun-Hyun;Han, Young-Sook;Lim, Sang-Dong;Lee, Myung-Ki
    • Journal of the Korean Society of Food Culture
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    • v.31 no.5
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    • pp.489-497
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    • 2016
  • Kunrak, a type of Korea traditional cheese, is made using Tarak, a yogurt produced with makgeolli as the fermentation source. Kunrak is produced by removing whey from Tarak, followed by drying process for safe storage and consumption over a longer period. In this study, we produced kunrak based on the method described in "Imwonsibyukji". Prepared Kunrak was ripening for 96 hours at 20, 30, and $40^{\circ}C$. In order to study characteristics of Kunrak, physiochemical properties (pH, acidity, water contents) and contents of metabolites (organic acids, sugars, amino acids, and fatty acids) were analyzed. During ripening, water contents decreased. The main organic acids in Kunrak were citric acid and lactic acid, and the main free sugar was lactose. Main amino acids were glutamate and phenylalanine, and main fatty acid was saturated capric acid. At later ripening, all metabolites increased immediately after preparation. The sensory evaluation score of overall preference was highest for Kunrak, which was ripening at $40^{\circ}C$ for 96 hours. This study was aimed to assay metabolites of Kunrak under various ripening conditions. The results provide basic data to produce conditions for standardized manufacturing of Kunrak.

Effect of NADH-Dependent Enzymes Related to Oxygen Metabolism on Elimination of Oxygen-Stress of Bifidobacteria (NADH요구 산소대사관련 효소가 bifidobacteria의 산소스트레스 제거에 미치는 영향)

  • Ahn, Jun-Bae;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.37 no.6
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    • pp.951-956
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    • 2005
  • Selection of oxygen-tolerant strains and elucidation of their oxygen tolerance mechanism were crucial for effective use of bifidobacteria. Oxygen-tolerant bifidobacteria were able to significantly remove environmental oxygen (oxygen removal activity) as compared to oxygen-sensitive strains. Most oxygen removal activity was inhibited by heat treatment and exposure to extreme pH (2.0) of bifidobacterial cell. NADH oxidase was major enzyme related to oxygen removal activity. Oxygen-tolerant bifidobacteria possessed high NADH peroxidase activity level to detoxify $H_2O_2$ formed from reaction of NADH oxidase. Addition of oxygen to anaerobic culture broth significantly increased activities of HADH oxidase and NADH peroxidase within 1hr and rapid increment of oxygen concentration was prevented. Results showed NADH oxidase and NADH peroxidase of oxygen-tolerant bifidobacteria played important roles in elimination of oxygen and oxygen metabolite $(H_2O_2)$.

Genetic Analysis of Sexual Life Cycle in Heterothallic Saccharomycopsis lipolytica (Heterothallic Saccharomycopsis lipolytica의 유성생활환(有性生活環)의 유전적(遺傳的) 해석(解釋))

  • Cho, Seok-Gum;Chung, Dong-Hyo
    • Applied Biological Chemistry
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    • v.29 no.1
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    • pp.3-9
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    • 1986
  • A yeast strains, CJ 2, CJ 7 and CJ 8, isolated from soil and contaminated choose, mated with authentic strains of Saccharomycopsis lipolytica and were identified as Saccharomycopsis lipolytica with mating A, B and B, respectively. The strain CJ 7 produced large amount of isocitric acid in glucose and n-hexadecane medium as compared with another strains. All strains produced larger amount of citric acid in n-hexadecane medium as compared with glucose medium, and citric acid production of diploids was greater than that of the parental haploid strains. The specific activity of isocitrate lyase in n-hexadecane grown cells was $15{\sim}20$ times greater than that in glucose-grown cells, but the specific activity of citrate synthetase was not so influenced by carbon source. Little correlation between citric acid production and the specific acitivity of these enzymes was noticed irrespective of strains and ploidy.

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The Actual Vegetation and Degree of Green Nat-urality in Gaya Mountain National Park (가야산 국립공원 지역의 현존식생 및 녹지자연도)

  • 김준선;김갑태;임경빈
    • Korean Journal of Environment and Ecology
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    • v.3 no.1
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    • pp.1-15
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    • 1989
  • To investigate the actual vegetation and the degree of green naturality(DGN) in Gaya Mountain National Park, forest communities were classified by phytosocialogical analysis according to physiognomy and Braun-Blanquet methods. Major forest communities were as follows: 1. Pinus densiflora community 1) P. densiflora-Rhododendron mucronulatum subcommunity 2) P. densiflora-Lespedeza maximowiczii subcommunity 3) P. densiflora-Sasa borealis subcommunity 4) P. densiflora-Carex siderosticta subcommunity 2. Quercus mongolica community group. 1) Q. mongolica-Rhododendron schlippenbachii community a) Typical subcommunity b) Vaccinium koreanum subcommunity 2) Q. mongolica-Sasa borealis community 3) Q. mongolica-Lespedeza maximowiczii community 3. Quercus serrata community 4. Carpinus laxiflora-Quercus serrata community 5. Rhododendron tschonoskii community 6. Lespedeza crytobotrya-Miscanthus sinensis var. purpurascens community The map of DGN was drawn according to the actual vegetaton map. The ratio of DGN 7 and 8 was 93.9% in Gaya Mountain National Park.

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