• Applied Microscopy
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• v.40 no.4
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• pp.253-259
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• 2010

The ultrastructure of sperms in testes of the pond smelt (Hypomesus nipponensis) was investigated using electron microscopes. The whitish testis was located between swim bladder and intestine. Especially, the left testis was larger than the right testis. The sperm was approximately $26\;{\mu}m$ in length. The sperm had an oval head and the acrosome was not found. The nucleus was about 400 nm in diameter and chromatin was incompletely condensed. The nuclear fossa deeply formed in sperm head and two centrioles were located in the fossa. The mitochondrium was observed only one in midpiece of the sperm and a motile flagellum consisted of an axoneme with a typical 9+2 pattern of microtubule. Also, the tail of the sperm has axonemal fins.

• The Korean Journal of Nuclear Medicine
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• v.21 no.1
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• pp.69-73
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• 1987

외상에 의한 횡경막파열은 신속한 진단이 매우 어렵다. 이는 임상증상 및 일반적인 방사선학적 소견이 특이성이 없고, 많은 다른 외상이 동반되어 임상적으로나 방사선학적으로 진단하기 힘들기 때문이다. 더우기 외상성 청경막파열은 좌측에 비해 우측에서는 매우 드물다. 저자들은 임상경사와 단순 흉부-X선 사진상에서 진단이 어려웠던 우측 외상성 횡경막 파열등 3예에 매해서 간신티그램을 시행하여 몇가지 특징적 소견을 관찰할 수 있었다. 즉 간신티그램 상에서 관찰된 횡경막파열의 특징적인 소견은 1) 청경막 탈출에 의한 간우엽의 융기 2) 융기부 기저부의 예각형성 그리고 3) 횡경막교약에 의한 띠모양의 냉소이었다. 그러나 이들 3가지 소견은 반드시 모두가 나타나지 않을 뿐만 아니라 같은 환자에서도 신티영상의 촬영방향에 따라 나타나는 수도 있고 나타나지 않을 수도 있었다. 그러므로 우측 횡경막내 외상성파열로 인한 간 등의 허니아를 진단하기 위해서는 다각도로 신티영상 촬영을 하여야 할 것이다.

• The Korean Journal of Zoology
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• v.35 no.1
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• pp.45-57
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• 1992

생쥐를 48시간 동안 결식시킨 후 유문결찰하여 급성미란을 유발시키고 미란형성부의 점막 내위선을 구성하는 각 세포의 구조변화와 IgA 분비세포의 분포를 관찰하였다. 미란부 점액분포의 변화는 표면상피의 손상 정도가 심해질수록 경계부의 PAS양성반응은 감소되었으며 중심부에서는 표면상피의 소실로 인하여 PAS 양성반응이 관찰되지 않았다. Glycocalyx 관찰을 위한 ruthenium red 반응에서도 경계부의 상피세포 손상이 심해짐에 따라 정단세포막 상층에 나타나는 양성반응은 감소되었고 측면과 기저면의 양성반응은 관찰되지 않은 것으로 보아 세포 손상시 tight junction은 파괴되지 않음을 알 수 있었다. 미란경계부의 미세구조변화로서, 상피세포는 손상이 진행될수록 세포질이 용해되어 공포가 형성되고 핵응축 및 정 단세포질의 점액원과립의 소실이 관찰되었으나 tight junction은 유지되었다. 반면에 점액경세포는 점액원과립의 수가 증가되고 조면소포체가 종창되어 있었다. 미란의 경계부와 중심부에서 벽세포는 다수의 소포가 출현한 후 세포내세관이 종창되었으며 그 후에 mitochondria가 파괴되었고 주세포는 주변의 벽세포가 커지면서 가장자리로 밀려나 크기가 정상보다 작아지고 조면소포체와 mitochondria가 종창을 나타내었다. IgA분비세포는 점막이 파괴된 미란부 점막하조직의 혈관 주변에 다수 존재하는 형질세포에서 확인되었다.

• The Korean Journal of Malacology
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• v.10 no.1
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• pp.1-8
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• 1994

동양달팽이속(Nesiohelix)에 속하는 종으로 한국에서는 유일하게 서식하고 있는 종으로알려진 동양달팽이(Nesiohelix samarangae)성체의 눈에 대하여 해부학적 및 미세구조관찰을 실시한 결과 다음과 같은 결론을 얻을 수 있었다. 동양달팽이의 눈은 각막, 무세포성의 수정체, 망막및 신경망 등으로 구성되어 있는 바 가장 복잡한 구조를 보이는 부분은 망막으로서 이의상피는 2종의 원주세포로 구성되어있다. 그 하나는 광수용세포로서 세포의 상부세포질이 원추형 또는 피라미드형의 돌기를 이루었고 그 유리표면에는 긴 감간(microvilli)이 무수히 많이 존재하고 있으며, 돌기를 이룬 세포질 내에는 용해소체의 활성이 높게 나타난다. 또한 광수용세포들의 상부세포질에는 이웃해 있는 색소세포들의 세포질 돌기들이 여러개 침입해 들어와 있다. 그리고 핵 주변의 세포질에는 수많은 photic vesicle들이 군집을 이루어 존재한다. 다른 한 종류의 세포는 색소세포들로서 광수용세포들과는 달리 세포의 상부에 원추형의 세포질 돌기가 없고 다만 그 유리표면을 따라 광수용세포의 것보다는 굵고 짧은 많은 감간이 존재하는데 두 종류의 세포들에서 뻗어나온 감간들은 서로 만나 때로 파상을 이루고 있다. 망막 상피세포층의 기저막 아래에는 신경망이 컵모양의 망막을 따라 존재한다.

• Applied Microscopy
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• v.33 no.1
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• pp.25-39
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• 2003

Cell differentiation and ultrastructural characteristics in the seminiferous epithelium of Myotis macrodactylus was investigated with the light and electron microscopes. Spermatogenesis has begun at April and finished at September. The nuclei of A spermatogonia (dark and pale type of spermatogonia) were oval, applied to the basal lamina, and surrounded by Sertoli cells. By comparison with other types of spermatogonia, the cell and nucleus of B type of spermatogonium is globular and larger than A types of spermatogonia. The nucleolus appears as a coarse and touches the nuclear membrane. The cell and nucleus of spermatocytes was globular and larger, but primary spematocyte is larger than secondary spermatocyte. Spermiogenesis was divided according to the level of fine structural difference, into Golgi, cap, acrosomal, maturation and spermiation phases; Golgi, cap, acrosomal and spermiation phases were further subdivided into steps of early and late phase respectively, and maturation phase has only one step. Hence, the spermiogenesis has been divided into a total of nine phases. In the change of karyoplasm, the chromatin granules are condensed at late Golgi phase and completed at spermiation phase. The sperm tail began to develop in early Golgi phase and completed in spermiation phase. The process of degeneration of spermatogenic cells in the seminiferous tubules was continually observed from October, before the beginning of hibernation, to hibernation phase (November, December, January, February, March). Immatured spermatogenic cells in the seminiferous tubules have been engulfed by phagocytosis of Sertoli cells during period of degeneration. It is deduced that the adaptative strategy serves as the mechanism to regulate the effective use of energy to prepare for long hibernation and regulation of breeding cycle.

• The Korean Journal of Nuclear Medicine
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• v.35 no.4
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• pp.241-250
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• 2001

Purpose: Vasospasm is a complication of aneurysmal subarachnoid hemorrhage (aSAH). We assessed the role of acetazolamide-enhanced brain perfusion SPECT (ACZ-SPECT) with Tc-99m ECD for predicting the prognosis of patients with aSAH. Materials and methods: Two SPECT studios (baseline with 740 MBq and ACZ-SPECT with 1480 MBq) with image subtraction were performed in 21 patients with aSAH. All patients had brain CT and angiogram. Vasoreactivity on ACZ-SPECT, perfusion defect on baseline SPECT, and vasospasm on angiogram were correlated with Hunt-Hess grading, extent of SAH (unilateral or bilateral) on CT, and clinical outcome. Vasoreactivity was considered decreased when cerebral/cerebellar uptake ratio difference from baseline SPECT to ACZ-SPECT was greater than 2SD of normal control values. Results: Decreased vasoreactivity was observed in 38% (8/21), perfusion defect in 81% (17/21), and vasospasm in 38% (8/21). The preserved vasoreactivity group showed better outcome scale (92%, 12/13) and the decreased vasoreactivity group showed poorer outcome scale (62.5%, 5/8) (p=0.014). Extensive SAH was more frequently seen in the decreased vasoreactlvlty group (87.5%, 7/8) than in the preserved vasoreactivity group (30.7%, 4/13)(p=0.017). The perfusion defect and vasospasm did not show good correlation with outcome scale, extent of SAH, and Hunt-Hess grading (p=ns). Vasoreactivity represented the patient's outcome better than the vasospasm in all of the vasoreactivity/vasospasm-mismatched cases (6 cases). Conclusions: Our data show that decreased vasoreactivity on ACZ-SPECT does not always represent vasospastic condition. But patients with decreased vasoreactivity reveal poorer outcome than patients with angiographic vasospasm do. Therefore ACZ-SPECT is a valuable, noninvasive test for predicting the prognosis of patients with aSAH.

• Applied Microscopy
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• v.29 no.3
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• pp.383-403
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• 1999

This study was performed to investigate the immunohistochemical and ultrastructural characterization of the choline acetyltransferase (ChAT)-immunoreactive nerve cells in the diagonal band of Broca of the rat basal forebrains, utilizing techniques of immunohistochemical and immunocytochemical microscopy. The ChAT-immunoreactivities were shown within neuronal cell bodies and processes by the light micoscope. According to cell shape and ratio of long axis vs short axis of cell body, the ChAT-immunoreaclive nerve cells in both vertical and horizontal limbs of the diagonal band of Broca were classified into 6 types. at the light microscopic level; round, oval, elongated, fusiform, triangular and polygonal types. As a result of the electron microscopic observation, the ChAT-immunoreactivated products appeared on the outer nuclear envelope, membranes of rough endoplasmic reticula (rER), free ribosomes and polysomes. Each cell type was subdivided into subtype I and II according to the several criteria such as volume of cell body, nuclear size relative to the cytoplasm, kinds and distribution of cell organelles and numbers and sorts of synapses. The subtype I of immnunoreactive nerve cells had large cell body and a small nucleus showing shallow indentations of nuclear evelope. In this subtype I with abundant cytoplasm, rER were well differentiated. Their long cisternae were parallelly ditributed and lamellated. One or two lamellar bodies and nematosomes were observed. The subtype II cell had small cell body and a large nucleus with deep indentations of nuclear envelope. In this subtype II with small cytoplasm, the rER were irregularly distributed and the lamellar body and nematosome were not found. A few axosomatic synapses in the subtype I and II were shown to be symmetric or asymmetric. The ratios of the symmetric synapse to the asymmetric one were investigated to be 1 : 2 and 1 : 4 in the subtype I and II, respectively. The axodendritic ones were almost asymmetric. But, the fusiform and triangular immunoreactive nerve cells were shown only to be subtype I. According to observations in this study, it is considered that the ultrastructural characterization in the 2 subtypes of each cell type may reflect the differences of the metabolic activities and projecting distances to the target cells.

• Korean Journal of Ichthyology
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• v.18 no.4
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• pp.311-318
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• 2006

Ultrastructure of germ cells, the cyst epithelial cells and interstitial cells during spermatogenesis of the stone flounder, Kareius bicoloratus (Pleuronectidae) sampled on the west coast of Korea were investigated by electron microscopic observations. In the primary spermatocyte, the synaptonemal complexes appear in the zygotene stage of the prophase during maturation division. In the growing testis, especially, the interstitial cells (Leydig cells) appear near the primary, secondary spermatocytes and spermatids. Well-developed interstitial cells (steroid hormone secreting cells) which are located in the interlobular space in growing testis have three morphological characteristics of a vesicular nucleus, mitochondria with tubular cristae and smooth endoplasmic reticulum. During spermatogenesis, the primary and secondary spermatocytes attach to the cyst epithelial cell (Sertoli cell) having an elongated ovoid or triangular nucleus and several mitochondria in the cytoplasm. In the growing testis, lipid droplets, the mitochondrial rosettes and glycogen particles appear in the cytoplasm of the cyst epithelial cells near the secondary spermatocytes and spermatids. Particularly, the mitochondria, endoplasmic reticulum, little lipid droplets and the large amount of glycogen particles are present in the cytoplasm of the cyst epithelial cell in the late growing testis. In the late stage of spermiogenesis, the proximal centriole is joined to the nuclear envelope, the distal centriole forms the basal body of the flagellum and gives rise to the axial filament of the flagellum. No acrosome of the sperm is formed as seen in other teleost fish. The head of the spermatozoon is approximately $3{\mu}m$ in length and its tail is about $30{\mu}m$ in length. The axoneme of the tail flagellum of the spermatozoon consists of nine outer doublet microtubules at the periphery and two centrial singlet microtubules at the center. The spermatozoon of this species has two axonemal lateral fins. Especially, the cyst epithelial cells which located near groups of gametes in the various stages, show three functions: nutrition, phagocytosis and steroidogenesis. Especially, the nuclei of cyst epithelial cells in the recovery stage of the testicular developmental stages appear to be irregular in shape after spermiation. Of three functions of the cyst epithelial cell, several characteristics of phagocytosis are showed in the cytoplasm of the cyst epithelial cells in the recovery stage of the testicular developmental stages. At this stage, therefore, it is assumed that the cyst epithelial cells are involved in degeneration and resorption of undischarged germ cells after spermiation.

• The Korean Journal of Nuclear Medicine
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• v.36 no.4
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• pp.244-254
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• 2002

Purpose: This study investigated the differences between technetium-99m ethyl cysteinate dimer (Tc-99m ECD) and technetium-99m hexamethylpropylene amine oxime (Tc-99m HMPAO) uptake in the normal brain by means of statistical parametric mapping (SPM) analysis. Materials and Methods: We retrospectively analyzed age and sex matched 53 cases of normal brain SPECT. Thirty-two cases were obtained with Tc-99m ECD and 21 cases with Tc-99m HMPAO. There were no abnormal findings on brain MRIs. All of the SPECT images were spatially transformed to standard space, smoothed and globally normalized. The differences between the Tc-99m ECD and Tc-99m HMPAO SPECT images were statistically analyzed using statistical parametric mapping (SPM'99) software. The differences bgetween the two groups were considered significant ant a threshold of corrected P values less than 0.05. Results: SPM analysis revealed significantly different uptakes of Tc-99m ECD and Tc-99m HMPAO in the normal brains. On the Tc-99m ECD SPECT images, relatively higher uptake was observed in the frontal, parietal and occipital lobes, in the basal ganglia and thalamus, and in the superior region of the cerebellum. On the Tc-99m HMPAO SPECT images, relatively higher uptakes was observed in subcortical areas of the frontal region, temporal lobe, and posterior portion of inferior cerebellum. Conclusion: Uptake of Tc-99m ECD and Tc-99m HMPO in the normallooking brain was significantly different on SPM analysis. The selective use of Tc-99m ECD of Tc-99m HMPAO in brain SPECT imaging appears especially valuable for the interpretation of cerebral perfusion. Further investigation is necessary to determine which tracer is more accurate for diagnosing different clinical conditions.

• The Korean Journal of Nuclear Medicine
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• v.37 no.5
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• pp.278-287
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• 2003

Purpose: The performance of nitroglycerin-challenged Tc-99m-MIBI quantitative gated SPECT for the detection of viable myocardium was compared with rest/24-hour redistribution Tl-201 SPECT Materials and Methods: In 22 patients with coronary artery disease, rest Tl-20l/ dipyridamole stress Tc-99m-MIBI gated/24-hour redistribution Tl-201 SPECT were peformed, and gated SPECT was repeated on-site after sublingual administration of nitroglycerin (0.6 mg). Follow-up gated SPECT was done 3 months after coronary artery bypass graft surgery. For 20 segments per patient, perfusion at rest and 24-hour redistribution, and wall motion and thickening at baseline and nitroglycerin-challenged state were quantified. Quantitative viability markers were evaluated and compared;(1) rest thallium uptake, (2) thallium uptake on 24-hour redistribution SPECT, (3) systolic wall thickening at baseline, and (4) systolic wall thickening with nitroglycerin-challenge. Results: Among 100 revascularized dysfunctional segments, wall motion improved in 66 segments (66%) on follow-up gated myocardial SPECT after bypass surgery. On receiver operating characteristic (ROC) curve analysis, the sensitivity and specificity of rest and 24-hour delayed redistribution Tl-201 SPECT were 79%, 44% and 82%, 44%, respectively, at the optimal cutoff value of 50% of Tl-201 uptake. The sensitivity and specificity of systolic wall thickening at baseline and nitroglycerin-challenge were 49%, 50% and 64%, 65% at the optimal cutoff value of 15% of systolic wall thickening. Area under the ROC curve of nitroglycerin-challenged systolic wall thickening was significantly larger than that of baseline systolic wall thickening (p=0.004). Conclusion: Nitroglycerin-challenged quantitative gated Tc-99m-MIBI SPECT was a useful method for predicting functional recovery of dysfunctional myocardium.