• Radiation Oncology Journal
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• v.4 no.1
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• pp.15-20
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• 1986

From 1979 to 1984, 39 local allograft irradiations were given to 29 patients: 10 irradiations were administered for prevention and 29 for reversal of acute rejection of transplanted kidney. Three doses of 150 cGy every other day were combined with high-dose of methylprednisolone pulse (1 gm/day) for 3 days. For prevention of acute rejection, local irradiation was delivered on the days 1, 3, and 5 after the transplantation, and for reversal, irradiation started after the diagnosis of acute rejection. Eight out of 10 patients irradiated for prevention had acute allograft rejection, and, what is more, there was no surviving graft at 15 months after transplantation. Reversal of acute rejection was achieved in $71\%$. When the pre-irradiation level of serum creatinine was below $5.5mg\%$, the reversal rate was $93\%$, but above $5.5mg\%$ the reversal rate was only $17\%$ (p<0.01). Reirradiation after failure was not successful. Among 15 reversed patients, $7(47\%)$ had subsequent rejection (s). The functional graft survivals at 6 month, 1, 2, and 3 year were $70\%,\;65\%,\; 54\%,\;and\;54\%$, respectively. Therapeutic irradiation resulted in better graft survival when serum creatinine was below $5.5mg\%$ (p<0.001) or when irradiation started within 15 days after the diagnosis of acute rejection (p<0.001).

• Reproductive and Developmental Biology
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• v.39 no.3
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• pp.89-95
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• 2015

The addition of growth factors and cytokines to in vitro culture (IVC) media could affect embryo development and the quality of the resulting blastocysts. The present study was performed to investigate the effect of porcine induced pluripotent stem cell (piPSC)-culture conditioned medium (CM) on the in vitro maturation (IVM) and development of parthenogentic embryos (parthenotes) in pigs. Cumulus-oocyte complexes (COCs) or activated oocytes were cultured in IVM or IVC medium supplemented with 0 (control), 25, or 50% of stem cell medium (SM) or CM, respectively. The maturation rate of CM-25% group was significantly improved when compared with control group (p<0.05), but that was not different among SM or CM groups. Blastocyst formation rate was significantly higher in CM-25% group (29.2%) than that of control (20.7%), SM-50% (19.6%) and CM-50% (23.66%, p<0.05). Cell number and the apoptotic cell index in blastocysts was significantly lower in SM-25% than in CM-25% group (p<0.05). The embryo quality related genes, OCT4, KLF4, TERT and ZFP42, were significantly increased in CM-25% group compared with control (p<0.05). In conclusion, the addition of 25% of CM to IVM and IVC medium positively influences not only the developmental potential also quality of parthenotes in pig.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.20 no.1
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• pp.1-15
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• 2015

Impacts of Sea Surface Temperature (SST) assimilation to the prediction of upper ocean temperature is investigated by using a regional ocean forecasting system, in which 3-dimensional optimal interpolation is applied. In the present study, Sea Surface Temperature and Sea Ice Analysis (OSTIA) dataset is adopted for the daily SST assimilation. This study mainly compares two experimental results with (Exp. DA) and without data assimilation (Exp. NoDA). When comparing both results with OSTIA SST data during Sept. 2011, Exp. NoDA shows Root Mean Square Error (RMSE) of about $1.5^{\circ}C$ at 24, 48, 72 forecast hour. On the other hand, Exp. DA yields the relatively lower RMSE of below $0.8^{\circ}C$ at all forecast hour. In particular, RMSE from Exp. DA reaches $0.57^{\circ}C$ at 24 forecast hour, indicating that the assimilation of daily SST (i.e., OSTIA) improves the performance in the early SST prediction. Furthermore, reduction ratio of RMSE in the Exp. DA reaches over 60% in the Yellow and East seas. In order to examine impacts in the shallow costal region, the SST measured by eight moored buoys around Korean peninsula is compared with both experiments. Exp. DA reveals reduction ratio of RMSE over 70% in all season except for summer, showing the contribution of OSTIA assimilation to the short-range prediction in the coastal region. In addition, the effect of SST assimilation in the upper ocean temperature is examined by the comparison with Argo data in the East Sea. The comparison shows that RMSE from Exp. DA is reduced by $1.5^{\circ}C$ up to 100 m depth in winter where vertical mixing is strong. Thus, SST assimilation is found to be efficient also in the upper ocean prediction. However, the temperature below the mixed layer in winter reveals larger difference in Exp. DA, implying that SST assimilation has still a limitation to the prediction of ocean interior.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.2
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• pp.109-116
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• 2012

This study was carried out to determine the forage quality, production, and regrowth of Phragmites communis as a native grass according to growth stages. Experiment 1 was conducted in Ansan from May 2009 to April 2010 (7 stages), and experiment 2 was conducted in Cheonan from May to November 2011 (10 stages). In experiment 1, forage quality decreased rapidly with advance of growth from May. When harvested on late May, July, September and April of the following year, the contents of crude protein were 7.7%, 4.6%, 3.7%, and 2.2%, respectively. Relative feed value (RFV) were 80.2, 65.1, 61.8 and 52.8, and lignin contents were 7.1%, 9.9%, 12.0%, and 13.2%, respectively. In experiment 2, significantly higher forage yields were observed when harvested from late June to mid July. On the other hand, forage quality decreased with delayed harvest as was in experiment 1. Good regrowth of Phragmites communis was observed when harvested earlier than in August. When harvested on late May, June, July, August, September, October and November, the dry matter (DM) yields were 7,329 kg/ha, 12,527 kg, 9,593 kg, 8,279 kg, 7,649 kg, 5,822 kg and 5,540 kg, and in vitro digestible DM (IVDDM) yields were 3,924 kg/ha, 5,264 kg, 4,273 kg, 3,322 kg, 3,352 kg, 2,195 kg and 1,887 kg, respectively. Forage quality grades of Phragmites communis were 4th grade in May, 5th grade from June to Sept., and 6th grade in Oct., Nov., or in Apr. of the following year. However, all regrown Phragmites communis ranked the 4th in quality. In conclusion, we recommend that Phragmites communis should be harvested from late June to mid July (no later than the end of July) to obtain good forage quality with digestible nutrient contents greater than rice straw. Regrowth of Phragmites communis was poor, and more than 70% of annual forage yields were out of the first harvest. Therefore, we recommend only one harvesting per year for good regrowth and stable production of Phragmites communis.

• Journal of Life Science
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• v.29 no.12
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• pp.1305-1313
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• 2019

Licochalcone (LC), isolated from the roots of Glycyrrhiza inflata has multiple pharmacological effects including anti-inflammatory and anti-tumor activities. To date, Licochalcone C (LCC) has induced apoptosis and inhibited cell proliferation in oral and bladder cancer cells, but lung cancer has not yet been studied. In addition, no study reported LCC-induced autophagy in cancer until now. The present study was designed to investigate the effect of LCC on gefitinib-sensitive and -resistant lung cancer cells and elucidate the mechanism of its action. The 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay data showed that LCC significantly inhibited cell viability in non-small cell lung cancer (NSCLC) HCC827 (gefitinib-sensitive) and HCC827GR (gefitinib-resistant) cell lines. Interestingly, Annexin V/7-aminoactinomycin D double staining and cell cycle analysis showed an apoptosis rate within about 20% at the highest concentration of LCC. LCC induced G2/M arrest by reducing the expression of the cell cycle G2/M related proteins cyclin B1 and cdc2 in NSCLC cell lines. Treatment of LCC also induced autophagy by increasing the expression of the autophagy marker protein microtubule-associated protein 1 light chain 3 (LC3) and the protein autophagy-related gene 5 involved in the autophagy process. In addition, LCC increased the production of reactive oxygen species (ROS), and the cell viability was partially restored by treatment with the ROS inhibitor N-acetyl-L-cysteine. In western blotting analysis, the expression of cdc2 was increased and LC3 was decreased by the simultaneous treatment of NAC and LCC. These results indicate that LCC may contribute to anti-tumor effects by inducing ROS-dependent G2/M arrest and autophagy in NSCLC. In conclusion, LCC treatment may be useful as a potential therapeutic agent against NSCLC.

• Korean Journal of Food Science and Technology
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• v.36 no.6
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• pp.893-899
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• 2004

Method for sample preparation and quantitative analysis of 19 permitted and non-permitted synthetic colors in foods was developed based on reversed-phase ion-pairing high performance liquid chromatography. For color extraction of samples, deionized water was added, and pH was appropriately adjusted with 1% ammonia water. Any undissolved matters were extracted with 50% ethanol or 70% methanol. Lipid in snacks was first removed using n-hexane with centrifugation, water was added to extract colors, followed by clean-up and concentration using Sep-Pak $C_{18}$ cartridge. Recovery efficiencies at known concentrations of 19 standard food colors spiked into foods were in 90.3-97.9% range far soft drink, 79.2-101.9% for candy, 84.1-103.4% for jelly, 86.4-100.8% for chewing gum, 83.5-103.4% for ice cream, and 78.5-95.6% for snack.

• The Korean Journal of Nuclear Medicine
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• v.32 no.4
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• pp.365-373
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• 1998

Purpose: The conventional gamma camera is not ideal for scintimammography because of its large detector size (${\sim}500mm$ in width) causing high cost and low image quality. We are developing a small gamma camera dedicated for breast imaging. Materials and Methods: The small gamma camera system consists of a NaI (T1) crystal ($60 mm{\times}60 mm{\times}6 mm$) coupled with a Hamamatsu R3941 Position Sensitive Photomultiplier Tube (PSPMT), a resister chain circuit, preamplifiers, nuclear instrument modules, an analog to digital converter and a personal computer for control and display. The PSPMT was read out using a standard resistive charge division which multiplexes the 34 cross wire anode channels into 4 signals ($X^+,\;X^-,\;Y^+,\;Y^-$). Those signals were individually amplified by four preamplifiers and then, shaped and amplified by amplifiers. The signals were discriminated ana digitized via triggering signal and used to localize the position of an event by applying the Anger logic. Results: The intrinsic sensitivity of the system was approximately 8,000 counts/sec/${\mu}Ci$. High quality flood and hole mask images were obtained. Breast phantom containing $2{\sim}7 mm$ diameter spheres was successfully imaged with a parallel hole collimator The image displayed accurate size and activity distribution over the imaging field of view Conclusion: We have succesfully developed a small gamma camera using NaI(T1)-PSPMT and nuclear Instrument modules. The small gamma camera developed in this study might improve the diagnostic accuracy of scintimammography by optimally imaging the breast.

• Nuclear Medicine and Molecular Imaging
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• v.43 no.5
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• pp.395-401
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• 2009

Purpose: We evaluated $^{18}F$-FDG PET/CT findings in initially diagnosed adenoid cystic carcinoma (ACC) of the head and neck in association with pathological subtype, staging, uptake comparison with squamous cell carcinoma (SqCC) and prognosis. Materials and Methods: The subjects were 16 patients with initially diagnosed ACC of head and neck who underwent pretreatment $^{18}F$-FDG PET/CT. Histological subtype (solid pattern vs. tubular/cribriform pattern), $SUV_{max}$ of size-matched SqCC of the head and neck as control group, disease-free survival (DFS) were compared with the $SUV_{max}$ of ACC of the head and neck. Results: Of total 16 patients, 6 had solid pattern and the remaining 10 had tubular/cribriform pattern. The $SUV_{max}$ were significantly higher in solid pattern group than in tubular/cribriform pattern group ($6.7{\pm}3.2$ vs. $4.2{\pm}0.9$, p=0.03). PET/CT found unexpected distant metastasis in 18.7% of patients (3/16) and changed the therapeutic plan in those patients. The $SUV_{max}$ of ACC was significantly lower than that of size-matched SqCC ($5.1{\pm}2.4$ vs. $13.6{\pm}6.0$, p<0.001). DFS was not significantly different according to the histological subtype. In contrast, patients with high $^{18}F$-FDG uptake ($SUV_{max}$ ${\geq}$6.0) had significantly shorter DFS than those with low $^{18}F$-FDG uptake ($SUV_{max}$ <6.0). Conclusion: $^{18}F$-FDG uptake of ACC of the head and neck is significantly associated with histological subtype and DFS. $^{18}F$-FDG PET/CT may be useful for detecting unexpected metastasis. Since $^{18}F$-FDG uptake of tubular/cribriform ACC compared with SqCC is relatively low, it is necessary to interpret PET images carefully in patients without alleged ACC.

• Journal of the Korean GEO-environmental Society
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• v.16 no.12
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• pp.45-52
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• 2015

With the rising interest in the environment, more attention on ecological restoration for damaged slope surface to restore its original state has been drawn. Generally, the most useful method is vegetation based spray work. This method uses green soil including sewage sludge, sawdust, paper sludge, and weathered granite soil. However, because there are neither accurate information nor test values about green soil, green soil is often lost by environmental factors such as rainfalls and strong winds. To solve the problem of green soil, it is necessary to prepare design standards about green soil, and conduct studies to deal with green soil loss in consideration of various variables including basic material property, soil quality of slope surface, and weather. This study was conducted in the mixture of green soil and eco-friendly soil stabilizer. With green soil, basic material property test and compaction test were conducted for the analysis on the basic characteristics of green soil. In the mixture with soil stabilizer at a certain ratio, we conducted shear strength test depending on the ratio in order to analyze the maximum shear strength, cohesion and the change in internal friction angles. Furthermore, in the mixture ratio of green soil and soil stabilizer, which is the same as the ratio in the shear strength test, an inclination of slope surface was made in laboratory for the analysis on erosion and germination rate. Finally, this study evaluated the most effective and economic mixing ratio of soil stabilizer to cope with neighboring environmental factors. According to the test, the shear strength of green soil increased up to 51% rely onto the mixing ratio of and a curing period, and its cohesion and internal friction angle also gradually increases. It is judged that the mixture of soil stabilizer was effective in improving shear strength and thereby increased the stability of green soil.

• Korean Chemical Engineering Research
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• v.53 no.2
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• pp.224-230
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• 2015

This study was conducted to investigate the effects of several variables on the durability of wood pellets fabricated with torrefied larch (LAR) and tulip tree (TUT) chips. Microscopic observation by scanning electron microscope-energy dispersive X-ray spectrometer was also performed to identify the surface of the wood pellets visually. In addition, torrefied-LAR and TUT pellets were fabricated with the addition of moisture, lignin, starch and protein as binders, and durabilities of the pellets were analyzed statistically. Durabilities of torrefied-LAR and TUT pellets were lower than one of non-torrefied-LAR and TUT pellets. Durabilities of both pellets fabricated with the wood chips, which were torrefied with $230^{\circ}C$ and 30 min, were the highest among all torrefaction conditions used in this study. From the microscopic observations, lignin was distributed broadly on the surface of non-torrefied wood pellets, whereas congregated partially on the surface of torrefied wood pellets. Durability of LAR pellets increased with the addition of moisture, but that of TUT pellets was reduced. Addition of binders contributed to increase the durability of LAR and TUT pellets. As a binder, lignin and protein were more effective than starch for improving the durability. In conclusion, mild torrefaction treatment, such as $230^{\circ}C$ and 30 min, might be an optimal condition to minimize the durability reduction of the LAR and TUT pellets. In addition, when torrefied woody materials with high and low specific gravities are used as a raw material for the production of durable wood pellets, it might be required to adjust moisture content and torrefaction conditions of woody materials, respectively.