• Title/Summary/Keyword: 기생수

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Studies on the Bionomic of Gonatocerus sp. y (Hymenoptera: Mymaridae), an Egg Parasite of the Green Rice Leafhopper, Nephotettix cincticeps Uhler (끝동매미충 난기생봉(Gonatocerus sp. y)의 생태에 관한 연구)

  • Kim Jeoung Bu;Kim Chang Hyo
    • Korean journal of applied entomology
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    • v.23 no.3 s.60
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    • pp.158-165
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    • 1984
  • These studies were carried out to investigate the bionomics of Gonatocerus sp. y (Hymenoptera: Mymaridae) , an egg parasite of the green rice leafhopper, Nephotettix cincticeps Uhler (Homoptera: Deltocephalidae) under laboratory and field conditions at Gyeongnam Provincial O.R.D. in Jinju from 1982 to 1983. The developmental period of each life stage was observed under four different temperature conditions from $15^{\circ}C\;to\;30^{\circ}C\;with\;5^{\circ}C$ increment. The development from egg to adult at 15^{\circ}C,\;20^{\circ}C,\;25^{\circ}C,\;and\;30^{\circ}C $ was completed in an average of 53, 25, 15, and 12 days and the females laid an average of 28, 48, 42, and 32 eggs respectively, with a maximum of 98. And most of the eggs were laid in the first two days after emergence. The time of adult emergence was determined under $25{\pm}1^{\circ}C$ condition. More than $76\%$ of the wasps emerged in the morning and the rest of them emerged in the afternoon. The average sex ratio between male and female was 1 : 5 when the wasps were developed from fertilized eggs. The longevity of female was greater than that of male when honey and water were provided as food, however, it was not when only water was supplied as food. The average longevities of females were 30 days at $15^{\circ}C$ and 15 days at $20^{\circ}C$ when honey and water were given as food. The peak precent parasitism of Gonatocerus sp. v on Nephotettix cincticeps in fallow fields was observed between late April and early May. The percent parasitism of the wasps In fallow fields was an average of 49\~59.9\% $ in Gyeochang, Namji and $23.2\~36\%$ in Namhae. The parasitic activities of the parasite had four peaks in paddy field, however, the highest peak was observed between late August and early September. The Percent e99 Parasitism of Gonatocerus sp. y was an average of $12.3\%$ on second the generation and $24.6\%$ of third genera lion of Nephotettix cinrticeps.

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Development of an in vitro culture method for harvesting the free-living infective larvae of Strongyloides venezuelensis (베네수엘라분선충 (Strongyloides venezuelensis Brumpt, 1934) 자유생활형 유충의 시험관 내 배양 기술 개발)

  • ;M.
    • Parasites, Hosts and Diseases
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    • v.36 no.1
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    • pp.15-22
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    • 1998
  • An in uitro culture technique was established for harvesting Strongwloides venezuelensis free-living infective larvae using a nutrient broth medium as a substitute for rat-feces in polyvinyl culture bags ($10{\;}{\times}{\;}12{\;}cm$). The egg hatch rate V) in sterile saline at different incubation temperatures (X) was expressed as the quadratic function, Y = $-0.192X^2$ + 8.673x - 19.550 (r = 0.901). The highest (100%) egghatch rate was observed at $25^{\circ}C$. A significant difference (p<0.05) in development rate W) of free-living infective larvae was observed between different concentrations of nutrient broth (X) which was highest (20.6%1 in 0.12% nutrient broth concentrations, incubated at $20^{\circ}C$ for 5 days [Y = $-864.032X^2$ + 245.995X- 0.560 (r = 0.875)]. Yields (Y) of infective larvae were observed relatively high when the culture medium was incubated at higher temperatures (X) which peaked at $25^{\circ}C$ (20.0%) than at lower temperatures. $15^{\circ}C$M (10.9%) and $20^{\circ}C$ (18.1%) [Y = $-0.189X^2$ + 8.387x- 72.795 (r = 0.981)]. The period W) required for the development of infective larvae decreased with higher incubation temperatures (X) [Y = $0.035X^2$ - 2.025X + 32.375 (r = 0.995)] The highest yield (19.2%) of infective larvae was obtained from culture bag inoculated with 15.000 eggs than with below and over 15,000 eggs in 0.12% nutrient broth and incubated at $25^{\circ}C$ for 4 days. The newly adapted culture method (from egg to third-stage larva) may be useful as a bio-bar/bioassay system for screening new chemical products, anthelmintics and pesticides, as well as for parasito immunological studies with Strongwloides species.

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Experimental life history of Echinostoma cinetorchis (이전고환극구흡충의 실험실 내 생활사)

  • 이순형;채종일
    • Parasites, Hosts and Diseases
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    • v.28 no.1
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    • pp.39-44
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    • 1990
  • The life history of Echinostoma cinetorchis was completed in the laboratory using Hippeutis cantors as the first and second intermediate host. The incubation for maturation of eggs and hatching of miracidia took 24 days at 28℃. On the 66th day after miracidial challenge, 16 snails were crushed and examined for the presence of E. cinetorchis larvae. The metacercariae were detected in all of the snails, and from three of them were found the rediae and cercariae. The morphological characteristics of the larvae and the experimentally obtained adults were compatible with that of E. cinetorchis.

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Ultrastructure of Vascular Meristems in the Rhizome of Botrychium ternatum (고사리삼 지하경의 유관속 분열조직 미세구조)

  • 소웅영
    • Journal of Plant Biology
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    • v.36 no.4
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    • pp.357-362
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    • 1993
  • To elucidate the origin of secondary growth in the rhizome of B. ternaturn, the developmental changes of vascular cambium was observed in ultrastructural features. The vascular cambium was gradually differentiated from procambium as in seed plants, but the cambial activity did not persist very long so that the cambial cells became a dormant state like fossil cryptogams. Dense cytoplasm of procambial cells became progressively sparse during the growth, and the tiny vesicles were fused to form numerous small vacuoles and then a few large vacuoles. These gradual changes and the occurrence of storage materials which was associated with the developmental stages might support the progressive differentiation of the cambial cells. In addition, the cessation of cambial activity could be indicated by the facts that late vascular cambial cells accumulate large lipid bodies and show very small peripheral cytoplasm and unlikely thickened cell wall, compared to other meristematic cells. Therefore. the vascular cambium showed the characteristics of both seed plants and fossil cryptogams from the view point of cambial ontogeny and activity.tivity.

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Egg Laying Capacity of Fibricola seonlensis in Mice and Rats (마우스 및 흰쥐에 있어서 Fibricola seoulensis의 산란력)

  • Hong, Sung-Tae;Kim, Chang-Hyun;Huh, Sun;Lee, Soon-Hyung
    • Parasites, Hosts and Diseases
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    • v.26 no.2
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    • pp.117-120
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    • 1988
  • The egg laying capacity of Fibritola seoulensis was observed in mice and rats. In mice, the mean number of eggs produced by an adult worm a day was 116 at the 1st week after infection, 123 at the 2nd week and 42 at the 3rd week. Thereafter, the capacity of about 20 eggs/worm/day was maintained up to one year period. In rats, it generally produced more eggs; 49 at 1 week, 250 at 2 weeks, 216 at 4 weeks, 327 at 6 months and 11 at one year after infection. It survived longer than a year in mice and rats.

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Comparative susceptibility of different cell lines for culture of Toxoplasma gondii in vitro (톡소플라스마 곤디의 세포내 배양에 있어서 세포 주에 따른 감수성 비교)

  • 박병규;문형로
    • Parasites, Hosts and Diseases
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    • v.31 no.3
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    • pp.215-222
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    • 1993
  • In order to establish a useful cell culture system for T gondii we compared the degree of proliferation of T gondii tachyzoites among 8 different cell lines: 2 kinds of normal animal cells (MDCK-canine kidney cells; Vero-monkey kidney cells) and 6 kinds of human tumor cells (A 549, PC 14-lung cancer cells; SNU 1, SNU 16. Mlm 45-stomach cancer cells; HL-60-promyelocytic leukemia cells), through morphological observation and 3H-uracil uptake assay. The degree of susceptibility to infection with T gondii tachyzoites was highest in A 549 and PC 14 cells, medium in Vero, HL-60, MDCK and SNU 1, and lowest in SNU 16 and MBm 45 cells. The kinetics of T gondii multiplication during the post-Infection 60 hours were higllly dependent upon the dose of tachyzoites administered and the duration among the 8 tested fur the growth and multiplication of T gondii in vitro.

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A Study on AC Modeling of the ESD Protection Devices (정전기 보호용 소자의 AC 모델링에 관한 연구)

  • Choi, Jin-Young
    • Journal of IKEEE
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    • v.8 no.1 s.14
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    • pp.136-144
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    • 2004
  • From the AC analysis results utilizing a two dimensional device simulator, the ac equivalent-circuit modeling of the ESD protection devices is executed. It is explained that the ac equivalent circuit of the NMOS protection transistor is modeled by a rather complicated form and that, depending on the frequency range, the error can be large if it is modeled by a simple RC serial circuit. It is also shown that the ac equivalent circuit of the thyristor-type pnpn protection device can be modeled by a simple RC serial circuit. Based on the circuit simulations utilizing the extracted equivalent circuits, the effects of the parasitics in the protection device on the characteristics of LNA are examined when the LNA, which is one of the important RF circuits, is equipped with the protection device. It is explained that a large error can result in estimating the circuit characteristics if the NMOS protection transistor is modeled by a simple capacitor. It is also confirmed that the degradation of the LNA characteristics by incorporating the ESD protection device can be reduced a lot by adopting the suggested pnpn device.

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PCR-RFLP patterns of three kinds of Metagonimus in Korea (국내에 존재하는 세 종류 메타고니무스속 흡충의 RCR-RFLP반응양상)

  • 유재란;정진성
    • Parasites, Hosts and Diseases
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    • v.35 no.4
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    • pp.271-276
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    • 1997
  • We tried to compare the three kinds oi Metagonimur species. M. Wokognulci, Ifetafonimus Miyata type, and M. tnknhashii, which were Know to be distributed in Korea with polymerase chain reaction based-restriction fragment length polymorphism (PCR- RFLP) patterns. We amplified the internal transcribed spacer 1 (ITSI) site of ribosomal RNA and mitochondrial cytochrome c oxidase I (mCOI) gene. The restriction patterns of ITSI gene loth Rsc I, ALu I and Msp I showed multiple fragmented bands of different sizes between three kinds of Metcgonimus. In case of mc01 gene, Rsc I and Alu I enzymes produced differentially fragmented band patterns. According to the parsimony analysis of PCR-RFLP patterns, the estimated genetic divergence between M Wokognwai and Metasoninus Miyata type was 0.034880, between Metusoninus Miyata type and M. tckc- hushii was 0.028098, between M. wokogawai and M. tnkahashii was 0.018179. It is suggested that Metasonimus Miyata type may be separate species and evolutionize at the older time than the other two species.

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Perspectives of methodology for Heighten Public Awareness of Dangsan Forest as Cultural Contents -Case of Alternative Trail of Jeoji Oreum Connected with Dang in Jeju Island- (문화콘텐츠로서의 당산숲 인식 증진을 위한 방법론 고찰 -당(堂)과 연계시킨 제주도 저지오름 대안 탐방로 모색을 사례로-)

  • Choi, Jai-Ung;Kim, Dong Yeob
    • The Journal of the Korea Contents Association
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    • v.13 no.11
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    • pp.924-934
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    • 2013
  • Traditional village forests in the Jeju Island represent unique cultural landscape with a history of more than several hundred years as a national cultural asset in Korea. There are 368 Oreums, parasitic volcanoes, and 391 shrines of Dang(Divine place) in Jeju. Currently, the shrines of Dang are damaged by the Jeju Olle trail path and road constructions. It's time for cultural consents to be obtained with locality and globality. And need to produce our own unique contents, glocal cultural contents. In this study, the establishment of trail path of Jeoji Oreum in relation to the Dang in the Jeju Island was investigated. By linking the Dangsan forest to the Jeoji Oreum trail path, it enables the path to contact with traditional cultural landscape. Rural villages in Jeju with the Oreum and Dang are expected to get an international attention as to have traditional cultural landscapes of Korea. It needs to be managed to retain their original form and in connection with the trail path.

Attempts to Transfer Immunity against Clonorchis in Nude and DS Mice (근교계 nude 및 DS 마우스 폐강삼출세포와 혈청의 간흡충 감염에 대한 면역이입의 시도)

  • Choe, Dong-Ik;Jeong, Dong-Il;Gang, Deok-Hui
    • Parasites, Hosts and Diseases
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    • v.29 no.4
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    • pp.371-380
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    • 1991
  • The effects of peritoneal exudate cells(PEC) and sera of athymic nude and DS mice infected with Clonorchis sinensis metacercariae or sensitized by injection of metabolic products into footpad on transfer of immunity against the fluke to the syngeneic mice were studied. There was no significant difference in eggs per gram pattern between the sensitized and control groups, and between nude and DS mice. However, the worm burdens were slightly greater in nude mice than in DS mice. Also, a few plaque forming cells were found in only DS mice given PEC and serum from Group II DS mice. In the light of these results, it is likely that PEC and sera of nude or DS mice which are deficient, at least partially, in the cellular immune system are unable to transfer immunity against C. sinensis to syngeneic recipients.

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