• The Journal of Korean Academy of Prosthodontics
• /
• v.53 no.4
• /
• pp.318-324
• /
• 2015

Purpose: This study compared shear bond strengths of five self-adhesive cements with phosphate monomer to zirconium oxide ceramic with and without airborn particle abrasion. Materials and methods: One hundred zirconia samples were air-abraded ($50{\mu}mAl_2O_3$). One hundred composite resin cylinders were fabricated. Composite cylinders were bonded to the zirconia samples with either Permacem 2.0 (P), $Clearfil^{TM}$ SA Luting (C), $Multilink^{(R)}$ Speed (M), $RelyX^{TM}$ U200 Automix (R), G-Cem $LinkAce^{TM}$ (G). All bonded specimens were stored in distilled water ($37^{\circ}C$) for 24 h and half of them were additionally aged by thermocycling ($5^{\circ}C$, $55^{\circ}C$, 5,000 times). The bonded specimens were loaded in shear force until fracture (1 mm/min) by using Universal Testing Machine (Model 4201, Instron Co, Canton, MA, USA). The failure sites were inspected under field-emission scanning electron microscopy. The data was analyzed with ANOVA, Tukey HSD post-hoc test and paired samples t-test ($\alpha$=.05). Results: Before and after thermocycling, $Multilink^{(R)}$ Speed (M) revealed higher shear-bond strength than the other cements. G-Cem $LinkAce^{TM}$ (G) showed significantly lower bond strengths after thermocycling than before treatment (P<.05), but the other groups were not significantly different (P>.05). Conclusion: Most self-adhesive cements with phosphate monomer showed high shear bond strength with zirconia ceramic and weren't influenced by thermocycling, so they seem to valuable to zirconia ceramic bonding.

• Journal of Adhesion and Interface
• /
• v.23 no.2
• /
• pp.25-32
• /
• 2022

There has been a growing demand for water based-type PSA due to environmental regulations for solvent-type PSA. And accordingly, there is a growing expectation as well for tackifiers used to compensate for the problem of deterioration of physical properties. Therefore, In this study, water-based tackifiers were synthesized by changing the contents of hard and functional acrylic monomers CHMA, IBOA, and AA. And these were added to the pressure-sensitive adhesive at 10 phr and their physical properties were compared. Tackiness slightly decreased as CHMA increased and IBOA decreased. Since the intermolecular bonding force increased due to the increase in AA content, the lower the AA content showed better results. Peel strength increased as the tackifiers were added because the fluidity of the polymer chain increased. And higher AA content showed better results because more hydrogen bonds were formed. The holding power tended to decrease as CHMA increased because the content of IBOA relatively decreased which has a large influence on the holding power. And higher AA content showed better results.

• Membrane Journal
• /
• v.18 no.2
• /
• pp.138-145
• /
• 2008

A series of anion exchange composite membranes were prepared and characterized for electro-dialysis process used in the removal of toxic anion and cation polutants in groundwater or wastewater. The membranes were prepared as follows; first, porous poly(ethylene) (PE) substrates were fully impregnated with monomer mixtures with various ratio of vinylbenzylchloride (VBC), divinylbenzene (DVB) and ${\alpha},\;{\alpha}$-azobis(isobutyronitrile) (AIBN). Second, they were thermally polymerized to yield crosslinked poly(VBC-DVB)/PE composite membranes. Finally, the membranes were treated in trimethylamine (TMA)/acetone to give $-N^+(CH_3)_3$-containing poly(VBC-DVB)/PE membranes. The basic membrane properties such as ion exchange capacity (IEC), electric resistance and water content of the resulting membranes were measured as a function of VBC/DVB and TMA/Acetone content. As a result, the composite membranes showed lower electric resistance, lower water content and higher IEC than commercial anion exchange membranes (AMX, Astom) due to thin PE substrates, indicating that the composite membranes could be successfully applied to the electrodialysis for water treatment.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
• /
• v.8 no.2
• /
• pp.49-59
• /
• 2002

The main function of plastic materials in food packaging is to preserve a food for safe transportation and storage. The interactions between food and plastic materials in food packaging have become increasingly important for food quality and safety because monomer, low molecular weight components, or additives of plastic packaging materials can migrate into a food. The use of antioxidants in plastic materials can help protect the degradation of film itself and retard the oxidation of a packaged food containing lipid, through the migration of antioxidant from the packaging to a product via an evaporation / sorption mechanism. Nowadays, antioxidant (BHT) impregnated plastic materials are used for commercial food packaging application with the intention of achieving an extended shelf life of food in USA. Alpha tocopherol, as one of the most important free radical scavengers, has been well known in biological systems. Moreover, the potential use of alpha tocopherol as an additive for polymers used in the packaging industry may offer the most positive perception from both consumers and manufacturers. Alpha tocopherol has been used as an antioxidant for polyolefin resins fabricated to both bottles and film and has applications in the food packaging industry as a replacement for BHT. Today, alpha tocopherol offers an attractive choice for use as an antioxidant in polymers. This paper provides an overview of antioxidant effectiveness and applications for its use by the food packaging industry based on the evaporation-sorption mechanism of a packaging model product, where quality is associated with lipid oxidation. Important analytical techniques for predicting antioxidant interaction between the package system and product are discussed.

• Korean Journal of Microbiology
• /
• v.52 no.1
• /
• pp.10-17
• /
• 2016

Enzyme immunoassay to analyze specific binding activity of antibody to antigen uses horseradish peroxidase (HRP) or alkaline phosphatase (AP). Chemical methods are usually used for coupling of these enzymes to antibody, which is complicated and random cross-linking process. As results, it causes decreases or loss of functional activity of either antibody or enzyme. In addition, most enzyme assays use secondary antibody to detect antigen binding activity of primary antibody. Enzymes coupled to secondary antibody provide a binding signal by substrate-based color development, suggesting secondary antibody is required in enzyme immunoassay. Additional incubation time for binding of secondary antibody should also be necessary. More importantly, non-specific binding activity caused by secondary antibody should also be eliminated. In this study, we cloned AP isolated from Escherichia coli (E. coli) chromosome by PCR and fused to) hAY4 single-chain variable domain fragment (ScFv) specific to death receptor (DR4) which is a receptor for tumor necrosis factor ${\alpha}$ related apoptosis induced ligand (TRAIL). hAY4 ScFv-AP expressed in E. coli showed 73.8 kDa as a monomer in SDS-PAGE. However, this fusion protein shown in size-exclusion chromatography (SEC) exhibited 147.6 kDa as a dimer confirming that natural dimerization of AP by non-covalent association induced ScFv-AP dimerization. In several immunoassay such as ELISA, Western blot and immunocytochemistry, it showed antigen binding activity by color development of substrates catalyzed by AP directly fused to primary hAY4 ScFv without secondary antibody. In summary, hAY4 ScFv-AP fusion protein was successfully purified as a soluble dimeric form in E. coli and showed antigen binding activity in several immunoassays without addition of secondary antibody which sometimes causes time-consuming, expensive and non-specific false binding.