• Proceedings of the Materials Research Society of Korea Conference
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• 2009.05a
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• pp.46.2-46.2
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• 2009

In vitro 상에서 골조직을 원활하게 재생하기 위해서는 3차원 지지체를 이용한 세포 배양과 세포 배양 시 세포의 형태와 기능을 유지/향상시키기 위한 인체 내 미세 환경 재현은 필수적이다. 따라서 본 연구에서는 뼈 성분과 유사한 생체 활성 물질인 hydroxyapatite (HA)와 생분해성 고분자인 poly $\varepsilon$-caprolactone (PCL)를 복합재료로 이용하여 내부 연결성이 우수한 골조직 재생용 3차원 지지체를 제작하였으며, 골 재생 능력 향상을 위하여 인체내 골조직의 기계적 미세 환경을 체외에서 구현한 새로운 형태의 perfusion bioreactor system을 개발/적용하였다. 또한 본 연구에서 개발된 perfusion bioreactor system의 생물학적 평가를 위해 MG63 (osteoblast like cell, 한국 세포주 은행)과 New Zealand White Rabbit에서 분리한 중간엽 줄기세포를 골조직 재생용 3차원 지지체에 파종하였다. 48시간 동안 안정화 후 perfusion bioreactor system을 이용하여 기계적 자극을 파종된 세포에 인가하였으며, 배양 기간 동안 세포의 증식 확인 및 형태학적 관찰을 실시하였다. 본 연구 결과, perfusion bioreactor system을 이용하여 기계적 자극을 인가한 실험군에서 세포의 증식 및 활성도가 대조군에 비해 우수함을 확인 할 수 있었다. 따라서, perfusion bioreactor를 이용한 세포 배양은 세포의 활성 향상 및 골조직 재생에 도움이 될 것으로 사료된다. 차후 perfusion bioreactor를 이용한 다양한 패턴의 자극이 골재생 능력 및 중간엽 줄기세포의 골 분화능에 미치는 영향에 대한 연구가 필요할 것으로 사료된다.

• Journal of the KSME
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• v.51 no.10
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• pp.39-43
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• 2011

이 글에서는 세포의 생리 및 대사에 미치는 물리적 환경과 기계적 자극의 역할과 그 기작에 대해 연구하는 메케노바이올로지(Mechanobiology)라는 새로운 융합학문 분야를 소개하고자 한다.

• Composites Research
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• v.32 no.5
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• pp.211-215
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• 2019

The cell culture process under in vitro condition is much different from the actual human body environment. Therefore, in order to precisely simulate the human body environment, a dynamic cell culture device capable of delivering mechanical stimulation to cells is essential. However, conventional dynamic cell culture devices require relatively complicated devices such as tubes, pumps, and motors, and the mechanical stimuli delivered is also simple. In this study, an electro-active polymer actuator as a driving component is introduced to design simply driven dynamic cell culture device without complicated components. The device is capable of delivering relatively complex mechanical stimuli to the cells.

• 기계와재료
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• v.23 no.2
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• pp.54-65
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• 2011

전기 활성 고분자 복합재는 전기적 자극을 가하여 기계적 움직임을 얻을 수 있고, 기계적 자극을 통해 전기적 신호를 얻을 수 있어 차세대 액추에이터 및 센서와 생물학적 조직과의 유사성으로 인공 근육 및 로봇분야의 응용소재로 최근 주목받고 있다. 본고에서는 전기 활성 고분자 복합재의 기본적이 개념과 함께 국내외 기술 동향을 살펴보았다. 또한 전기 활성 고분자 복합재에 있어 핵심내용인 전기활성 소재의 종류 및 작동원리, 전극소재, 이를 이용한 센싱 및 액추에이팅 구동특성과 응용에 대해 소개하고 마지막으로 현 시점에서의 전기 활성 고분자 복합재 응용에 있어 문제점과 이를 해결하기 위한 연구방안에 대해 언급한다.

• Aerospace Engineering and Technology
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• v.8 no.1
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• pp.154-161
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• 2009

Generation of pure and controllable excitation waves to excite the natural fast-growing instability waves has always been a difficult task. For this purpose, a device that is capable of supplying co- and counter-swirl was designed, fabricated, and applied. To incorporate a novel, robust mechanical excitation device that is capable of producing disturbances, mechanically and passively, a novel device was designed and built.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.12
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• pp.1121-1127
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• 2013

This study deals with experimental studies on electromyogram (EMG) measurements and functional electrical stimulation (FES) for the rehabilitation of a shoulder-joint. Based on the structure, motion, and main functions of the musculoskeletal system in a shoulder-joint, the muscles playing a major role for the motion in the sagittal plane were selected for the experiment. First, the surface electromyogram of the main muscles was measured according to the joint angle. The results showed that the change in the surface EMG was linearly proportional to the change in the joint angle. Second, the joint angle was measured during FES at shoulder muscles. The results showed that the joint angle increased as the FES current increased in a certain range of FES. It was confirmed that the willingness of muscles to move could be detected by measuring EMG and that the generation of muscle tension could be assisted by FES for active rehabilitation.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1411-1414
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• 2008

It is reported that mechanical stimulation takes a role in improving cell growth in skeletal system. And various research groups have showed that developed bioreactor to stimulate cell-seeded and threedimensional scaffold. In this study, we designed a custom-made bioreactor capable of applying controlled compression to cell-seeded agarose gel. This device consisted of a circulation system and compression system. In circular system, culture chamber was sealed for prohibiting contamination and media solution was circulated by pump. In compression system, mechanical stimuli were controlled by LabVIEW software and mechanical transfer system. Cell-encapsulated agarose gels were cultured for up to 7 days. There were significant differences between the number of cells grown in dynamic cell culture and in static cell culture from 3 days to 7 days.

• 대한치과교정학회:학술대회논문집
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• 1997.11a
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• pp.37-37
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• 1997

• Proceedings of the KSME Conference
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• 2007.05a
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• pp.1063-1068
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• 2007

The present study describes the effects of music and vibroacoustic stimuli to the relaxation of human body. We have carried out the experiment on 6 human subjects of which are composed 3men and 3women. We have investigated the electroencephalogram(EEG) of all subjects before and after the stimuli of which are made a strong noise or the meditatiom music and the acoustic vibration. The vibroacoustic device has transmitted meditation music as vibration between 20Hz and 250Hz to the body. From the experimental results, we made sure the effects that the meditation music and vibroacoustic stimuli influenced the stress reduction of human body for good as alpha-wave was increased continuously during the good stimuli and after that.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1401-1403
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• 2008

Considering that human body is continuously experiencing mechanical stimuli due to daily activities, the micro-physical environments of cells/tissues should be considered for the successful outcomes in tissue engineering and/or related researches. Obviously, there are many factors involved in cell-based researches. In this presentation, the current trends and some of outcomes are introduced. Through this studies, the roles of mechanical engineering in relation to medical/biological researches are to be emphasized.