• Korean Journal of Poultry Science
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• v.35 no.1
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• pp.57-62
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• 2008

The study was conducted to evaluate the effects of dietary organic acid mixture(lactic acid 12%, formic acid 9%, citric acid 5%, butyric acid 5% and phosphoric acid 6%) on growth performance, organ weight, blood immunological parameter and intestinal villi morphology in broilers. Dietary treatments included 1) CON (basal diet), 2) OA1 (basal diet + 0.1% organic acid mixture) and 3) OA2 (basal diet + 0.2% organic acid mixture). Four hundred eighty hatched Arbor Acre broiler chickens were housed in 24 pens of 20 birds each. Eight replicate pens were allotted into each treatment by completely randomized design. Daily weight gain, feed intake and feed/gain were not significantly different among the treatments. In organ weight, that of gizzard was significantly heavier in OA2 than CON (Linear effect = 0.034). However, those of spleen and intestine were not significantly different among the treatments. Blood immunological parameters such as RBC, WBC and lymphocyte were not significantly difference. In intestinal villi morphology, villi length was increased in OA1 compared to CON (Quadratic effect = 0.050). In conclusion, 0.2% organic acid mixture was effective to improve gizzard weight of broilers.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.2
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• pp.131-138
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• 2013

The present study was conducted with three ruminally cannulated non-lactating Holstein cattle in a $3{\times}3$ Latin square design to determine the fermentation characteristics and effective degradability (ED) in the rumen, as well as the whole tract digestibility of whole crop silage based total mixed ration (TMR) in comparison with conventional separate feeding of concentrate and rice straw for late fattening Hanwoo steers. The cattle in each group were fed separate feeding of concentrate and rice straw (control), whole crop barley silage based TMR (BS-TMR) or whole crop rye silage based TMR (RS-TMR). The ruminal fermentation characteristics such as pH, ammonia-N concentration and total volatile fatty acid were not affected by the experimental diet. The molar portion of acetate ($C_2$) was lowest in rumen fluid 1 h after feeding when cattle were fed BS-TMR (p<0.033). Molar proportions of propionate ($C_3$), butyrate and $C_2/C_3$ were not influenced by the experimental diet. There were no differences in effective degradability or whole tract digestibility of feed components among any of the experimental diets. The data obtained from the metabolism trial indicate that the feeding value of TMR with BS or RS is equal to that of a conventional separate feeding of concentrate and rice straw.

• Korean Journal of Poultry Science
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• v.29 no.4
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• pp.249-253
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• 2002

A 2 ${\times}$ 2 factorial experiment was conducted to determine the effect of cecectomy and urea supplementation on nitrogen utilization In growing chicks. Birds were either cecectomized or shamoperated and fed a 19% protein diet with or without 1% urea supplementation. All chicks were provided ad libitum access to feed and water throughout the six-day experimental period. In the cecectomized chicks, fred efficiency was significantly decreased when fed a 19% protein diet supplemented with 1% urea (P<0.05). When chicks were fed a 19% protein diet, cecectomy significantly shortened gastrointestinal passage time (GPT) of food (P<0.05). However, the GPT was significantly lengthened in chicks fed a 19% protein diet supplemented with urea (P<0.05). Regardless of diets, cecectomized chicks increased the moisture contents of the excreta (P<0.05) and feeding a 1% dietary urea also significantly increased (P<0.05). Cecectomy significantly decreased uric acid N excretion by about 30 mg per 100 g body weight per day (P<0.05) and uric acid N/ total N excretion when fed both diets (P<0.05). The present study suggests that cecectomy improves nitrogen utilization rate by decreasing uric acid excretion in growing chicks. These results are in good agreement with those obtained previously in a cecectomy study of adult chickens.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.8 no.5
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• pp.1266-1272
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• 2007

The current study was conducted to investigate the effects of dietary complex probiotics supplementation on growth performance, nutrient digestibility, blood characteristics and noxious gas emission of manure slurry in growing pigs. A total of forty eight pigs with an initial body weight of 20.12 kg were allotted to three dietary treatments (two pigs per pen with eight pens per treatment). Dietary treatment included: 1) CON (basal diet), 2) Pro1 (basal diet + 0.1% complex probiotics) and 3) Pro2 (basal diet + 0.2% complex probiotics). The experiment was lasted six weeks. Through the entire experimental period, ADG was increased with the increased complex probiotics supplementation level (linear effect, P<0.05). However, neither ADFI nor gain/feed was influenced by the dietary treatments. Complex probiotics supplementation increased DM digestibility (linear effect, P<0.05). Also, the N digestibility was improved, with the Pro1 treatment showed highest value (linear and quadratic effect, P<0.05). Supplementation of complex probiotics did not affect the WBC, RBC, lymphocyte and BUN concentrations in blood. The $NH_3-N$ emission from manure slurry was decreased with the increased level of complex probiotics supplementation (linear and quadratic effect, P<0.05). Similarly, $H_2S$ emission of manure slurry was also decreased significantly when complex probiotics was included in diet (linear effect, P<0.05). In conclusion, dietary supplementation of Complex probiotics can increase growth performance and decrease noxious gas emission of manure slurry in growing pigs.

• Korean Journal of Environment and Ecology
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• v.28 no.5
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• pp.495-499
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• 2014

To search for myrmecochorous plant species, related ant species and diaspore selecting behaviour, collecting fruits and diaspores of four Corydalis species, cafeteria experiments, and the related ant species were studied from May 2013 to July 2014. Four Corydalis species recording in Korea were proved myrmecochorous (seed attached elaiosome) plants newly. Elaiosome shape of these species is spatulate-shaped, but their size is varied considerably in length and width. Significant differences is proved between four plant species in weight of diaspores, seeds and elaiosomes, and elaiosome ratios. Mean values of diaspores weight showed 4.08 mg, 2.56 mg, 2.49 mg and 2.06 mg in Corydalis grandicalyx, C. remota, C. turtschaninovii for. pectinata and C. turtschaninovii var. linearis, respectively. Mean values of seed weights showed 3.68 mg, 2.03 mg, 1.90 mg and 1.67 mg in Corydalis grandicalyx, C. remota, C. turtschaninovii for. pectinata and C. turtschaninovii var. linearis, respectively. Elaiosome ratio of C. turtschaninovii var. linearis was the highest, followed by C. remota, C. turtschaninovii for. pectinata and C. grandicalyx. Ant's preference for the diaspores is ordered as same as elaiosome ratio's order. Major ant species transporting the diaspores of Corydalis species are Formica japonica, Lasius alienus, Lasius japonicus and Tetramorium tsushimae. Formica japonica transports the diaspores most frequently. Ant's preference for the diaspores among plant species or individuals might be influenced on the survival rates of concerned species. Further studies on the myrmecochorous plant and related ant species, cafeteria experiments should be needed.

• Journal of Animal Science and Technology
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• v.44 no.3
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• pp.341-350
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• 2002

Twenty four Hanwoo bulls(mean initial body weight 162$\pm$8.1kg) were employed to induce the regression equations on body weight gain and dry matter intake. The diets were fed in a 3${\times}$4 factorial arrangement; three roughage sources(rice straw, hay, corn silage) and four roughage ratios in ration(100, 60, 40, 20%). Bulls were alloted by incomplete turn over design with four replications. Daily body weight gain was greatest in bulls fed the corn silage as a roughage source and the 20% of roughage ratio, respectively. Bulls fed hay or corn silage only as feed kept in their body weight gain during the whole experimental period, however those fed rice straw only were in a negative body gain hereafter fatteningⅠperiod(350kg of body wt.). Dry matter intake per metabolizable body weight was decreased with increasing body weight. Dry matter intake was greatest in bulls fed the corn silage as a roughage source and the 20% of roughage ratio, respectively. The regression equations on daily body weight gain(DG, kg) and dry matter intake(DMI, kg/day) according to body weight(BW, kg) of Hanwoo bulls were derived as follows; DG = －0.842 + 17.5DMI/$BWkg^{0.75}$ DMI = 4.125 + 0.013BW + 1.075DG － 0.045NDF where, NDF is the percentage of neutral detergent fiber in ration.

• Journal of Nutrition and Health
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• v.36 no.10
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• pp.1030-1035
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• 2003

This study was conducted to evaluate the safety of herbicide-resistant rice, a genetically modified organism (GMO) developed by the Rural Development Administration, in Sprague-Dawley rats. The nutrient content of herbicide-resistant polished and brown cooked rice was compared with that of conventional Ilpum polished and brown cooked rice to assess composition equivalence. Compositional analysis was performed to measure proximates, fiber, and minerals before animal feeding. Growing male rats were fed one of the following four diets for six weeks: Ilpum polished cooked rice (IP) and Ilpum brown cooked rice (IB) as a non-GMO and herbicide-resistant polished cooked rice (GP) and brown cooked rice (GB) as a GMO. We checked clinical symptoms (anorexia, salivation, diarrhea, polyuria, anuria, fecal change) every day, food intake, change of body weight twice a week, and serum biochemistry and organ weights after 6 weeks of experimental feeding among the four groups. Nutrient content of the herbicide-resistant rice was similar to that of the non-transgenic control and was within the published range observed for non-transgenic rice. We could not find any significant difference in the above-mentioned items as the index to be checked in the animals fed the GMO. These results suggest that the nutrient content of genetically modified herbicide-resistant rice is compositionally equivalent to that of conventional Ilpum rice and that growing male rats fed herbicide-resistant rice are no different from those fed Ilpum rice, non-GMO for 6 weeks.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2002.11a
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• pp.64-84
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• 2002

These studies were conducted to evaluate the Properties of lactic acid Producing bacteria(LAB), isolated from broiler and laying hens cecum and select the optimum strains to improve the performance, environment of poultry house, immunity, and intestinal microflora of broiler and laying hens. In experiment I , 23 LAB strains were isolated from broiler and laying hens cecum as a colony form. Six strains were selected by acid tolerance, bile salt tolerance, viability, enzyme release, antagonism, and antibiotics susceptibility. In Experiment II, selected LABs from Ex. 1 were conducted to investigate the effects of feeding various Lactobacillus on performance, nutrients digestibility, intestinal microflora, villi development and observation of epithelium surface, blood chemicals and fecal noxious gas of broiler chicks. One thousand eighty one day old broiler chicks were fed into Lactobacillus crispatus avibrol(LCB), Lactobacillus reuteri avibro2(LRB), Lactobacillus crispatus avihen1(LCH), and Lactobacillus vaginalis avihen2(LVH) at the level of 10$^4$ and 10$\^$7/cfu/g diet. Weight gam of chicks fed Lactobacillus tended to increase from the first week and was higher from 50 to 100g in Lactobacillus treatments than control. Feed intake and feed conversion were not statistically different of all treatments. Dry Matter digestibility of Lactobacillus treatments was prone to improve compared to that of control, but was not significantly different. Protein and Ca digestibility were also tended to improve in Lactobacillus treatments relative that of control. Lactobacillus treatments showed improved tendency in crude ash and fat compared to those of control, whereas phosphorus digestibility was not consistency. Nutrients digestibilities of bird fed LCH were superior to those of other treatments, It showed significantly higher in Ca and P digestibility than control(P〈0.05). Total Lactobacillus spp. of birds fed various Lactobacillus was significantly higher in illeum for five weeks(P〈0.05), but was not different at cecum. Yeast was thought to be not completely attached to intestinal lumen for one week. However, total number of yeast was significantly increased in cecum and illeum of three weeks old chicks (P〈0.05). The number of anaerobes exhibited to tendency the increase in Lactobacillus treatments from one week old of age at both ileum and cecum.

• Journal of Animal Science and Technology
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• v.47 no.5
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• pp.721-730
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• 2005

Effects of levels of Bomboo Vinegar Liquids(BVL) on growth performance, serum profile and meat quality in employing 90 pigs were investigated. Ninety pigs were allocated into 3 groups and fed by dietary levels of BVL 0(control), 2.0 and 4.0% were included in experimental diets of each of the groups. Concentrated diet was supplemented with 2.0 and 4.0% BVL. The results showed that the daily weight gains and the feed conversion increased significantly(p<0.05) in 2.0% BVL and the feed intake increased slightly. The glucose and the cholesterol contents at the serum decreased significantly(p<0.05) in 2.0 and 4.0% BVL. On the other hand the total protein concentration and the blood urea nitrogen(BUN) increased significantly(p<0.05) in 4.0% BVL. The carcass weight increased significantly(p<0.05) in 4.0% BVL, and back fat thickness had decreased significantly(p<0.05) in 2.0 and 4.0% BVL. The carcass grade tended to increase in 2.0 and 4.0% BVL. The total fat content of loin tended to increase in 2.0 and 4.0% BVL while the cholesterol content decreased significantly(p<0.05). The pH had lower slightly in 2.0 and 4.0% BVL, the lightness and the yellowness of the meat color had higher significantly(p<0.05) while the redness decreased. Shear force values had lower significantly(p<0.05) in 2.0 and 4.0% BVL. For the fatty acid composition, the saturated fatty acids decreased significantly(p<0.05) in 2.0 and 4.0% BVL while unsaturated fatty acids(oleic acid and linoleic acid) increased significantly(p<0.05). For the sensory evaluation, 2.0 and 4.0% BVL had a significant(p<0.05) effect of diminishing the odor and improving the appearance. The taste was also significantly(p<0.05) improved. In conclusion the 2.0% BVL had a positive effect on the feeding performance, the carcass grade and the meat quality characteristics therefore 2.0% BVL is the appropriate supplemental levels of BVL for finishing pigs.

• Korean Journal of Poultry Science
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• v.29 no.3
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• pp.195-204
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• 2002

Two experiments were conducted to study the effects of nutrient level and feeding method of split diets for a.m. and p.m. on laying hen performance, feed cost and eggshell quality. In experiment 1, 384 ISA Brown layers of 30∼38wk of age were assigned to four treatments which comprise of three replicates each containing 32 birds. The control(C) was fed a conventional single diet throughout the day and split diet groups(T1, T2 and T3) were offered high energy/protein-low Ca diets, and low energy/protein-high Ca diets in a.m.(04:00∼15:00) and p.m.(15:00∼21:00), respectively. In the split diet groups, daily ME and CP consumption, and feed cost were significantly reduced(P<0.05) compared to the C, while the hen-day egg production, average egg weight and daily feed intake were not different among treatments. Due to the reduced daily ME and CP intakes and feed cost, the conversions of feed, ME, CP and feed cost required per day and per kg egg mass were also significantly improved(P<0.05) in the split diet groups. Eggshell qualities (egg specific gravity, egg breaking strength and eggshell thickness) were improved(P<0.05) by split diet feeding. As the Ca level of the p.m. diet increased. In Experiment 2, 384 ISA Brown layers of 50∼58 wk of age were used in three treatments and each treatment was represented by four replicates each containing 32 birds. The control(C) was fed a conventional single diet throughout the day and split diet group(T1) was offered high energy/protein-low Ca diets, and low energy/protein-high Ca diets in a.m.(04:00∼l5:00) and p.m.(15:00∼21:00), respectively. T2 group was fed the diet mixed (50:50) with the a.m. diets in mash and p.m. diet in pellet used T1 group. In T1 and T2 groups, daily feed intake and average egg weight were significantly reduced(P<0.05) compared to the C, while the hen-day egg production was not influenced by the feeding system. Daily ME and CP consumption, and feed cost were reduced(p.0.05), and the conversions of ME, CP and feed cost required per egg were also significantly improved(P<0.05) in T1 and T2, while the conversions of feed, ME, CP and feed cost required per kg egg mass were not different to the C. Eggshell qualities of T1 and T2 were improved(P<0.05) compared to the others. It was concluded the feed and nutrients consumption, feed cost per day or per kg egg mass could be reduced by introducing split diets for a.m. and p.m. and the feeding method of mixed diet of split diets were also convenient and effective for sparing feed cost and improvement of eggshell quality.