• Journal of the Korean Chemical Society
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• v.41 no.2
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• pp.98-104
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• 1997

A simple, accurate and reproducible capillary electrophoresis(CE) assay has been developed for the determination of berberine, cinnamic acid, and glycyrrhizin which are used in traditional Korean medicinal preparations. Separation of these compounds was performed in 20 mM phosphate buffer(pH 7.5) and acetonitrile(75:25, v/v) using a bare fused silica capillary($57 cm{\times}75 {\mu}m$ i.d.) at 25$^{\circ}C$. With the electric field of 350 V/cm, the time needed for the separation of berberine, cinnamic acid and glycyrrhizin was within 13 min. Calibration curves were linear for 1∼100 ${\mu}g/mL$ berberine, 0.3∼100 ${\mu}g/mL$ cinnamic acid and 2.5∼100 ${\mu}g/mL$ glycyrrhizin. The ranges of relative standard deviations(n=5) for those samples were between 0.96∼2.35%. The limits of detection(S/N=3) for berberine, cinnamic acid and glycyrrhizin were 0.5, 0.1 and 2.0 ${\mu}g/mL$, respectively. The numbers of theoretical plates were 181,000(berberine), 88,000(cinnamic acid) and 169,000(glycyrrhizin), while they were 3,100∼4,800 in HPLC.

• Fashion & Textile Research Journal
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• v.15 no.2
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• pp.286-293
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• 2013

This study investigates the attachment of glycyrrhizin to fabric using an X-ray photoelectron spectrophotometer( XPS). XPS spectra analysis showed that carbon content on treated fabrics with 0.2% glycyrrhizin increased to 2.699% for silk, 2.829% for nylon, 1.505% for cotton, respectively. The results show that glycyrrhizin is absorbed on treated fabrics. The glycyrrhizin extraction method makes radix glycyrrhizae powder 10g treat the first and the second treatment with ethanol, remove impurities on $75^{\circ}C$; subsequently, it is treated for 10 hours with ethanol 75% on $85^{\circ}C$ and lyophilizated. As the result, glycyrrhizin is extracted 1.7g in GL-I, 1.1 g in GL-II. As the result of abstracting glycyrrhizin with two methods, pure glycyrrhizin was abstracted 45.9% in GL-I, 74.9% in GL-II. GL-I, GL-II; in addition, glycyrrhizin( Japan) on TLC plate was separated in Rf 0.6. By GL-II extract method, this experiment obtained glycyrrhizin 15 g treated in a bath ratio set to 1: 100. Silk fabric was treated at $80^{\circ}C$, 60 min. in, nylon fabric $10^{\circ}C$, 70 min., and cotton fabric $30^{\circ}C$, 80 min.; subsequently, silk, nylon, cotton fabrics showed a 99.9% antibacterial activity for Staphylococcus aureus and Klebsiella pneumoniae.

• Proceedings of the Membrane Society of Korea Conference
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• 1999.04a
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• pp.105-108
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• 1999

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.98-98
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• 2018

Glycyrrhizin은 감초의 주성분으로 많은 생리활성을 나타내는 성분이다. 감초를 복용하게 되면, 글리시리진은 장내에 서식하고 있는 장내세균에 의해 glycyrrhetic acid로 대사된다. 하지만, 지금까지 대사반응에 관여하고 있는 균주가 분리 후 자원화되지 않았다. 본 연구를 통해 사람의 대변으로부터 glycyrrhizin을 대사하는 균주로 분리된 strain GLY의 16S rRNA 유전자를 분석한 결과, Enterococcus faecium과 99%의 상동성을 보여, Enterococcus faecium GLY (Genebank No. MH048988)로 명명하였다. 대사활성의 특성을 검토하기 위하여 time course, 기질의 농도에 따른 대사활성의 변화, 근연 균주와 대사활성 비교를 실시하였다. Time course 실험에서 GLY균주가 증식함에 따라 기질인 glycyrrhizin은 줄어들고, 대사산물인 glycyrrhetic acid는 새로 생성되었다. 또한, 기질의 농도에 따른 대사활성의 차이 검토를 위해 여러 농도를 처리하여 배양하였을 때, 1.0mM을 처리한 샘플에서 최대값의 대사물 농도를 보였다. Enterococcus faecium와의 근연균주를 이용하여 glycyrrhizin 대사활성을 측정한 결과, strain GLY 균주가 가장 큰 대사능을 가지고 있었다. 본 연구는 앞으로 glycyrrhizin 생체이용 및 대사 연구를 위한 기초연구가 될 것이며, 장내세균에 의한 생약성분의 대사를 이해하는데 도움이 될 수 있다.

• YAKHAK HOEJI
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• v.40 no.1
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• pp.84-89
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• 1996

This study was done to investigate the effect of glycyrrhizin on the lethality induced by galactosamine and lipopolysaccharide coadministration in mice. Glycyrrhizin was injecte d intravenously as a multiple dose at 20, 15, 10, 5, and O hr before galactosamine and lipopolysaccharide coadministration. Lethality and tumor necrosis factor (TNF${\alpha}$) level in serum were surveyed as markers of glycyrrhizin effect. Glycyrrhizin had no effect on the lethality induced by galactosamine and lipopolysaccharide when glycyrrhizin was administered as a single dose. Glycyrrhizin reduced the lethality induced by galactosamine and LPS in dose-dependent manner when glycyrrhizin was administered as a multiple dose at 20, 15, 10, 5 and O hr before galactosamine and lipopolysaccharide coadministration. Glycyrrhizin reduced the serum TNF${\alpha$ level.

• Analytical Science and Technology
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• v.13 no.4
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• pp.504-510
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• 2000

The impurity profiles from the raw materials of glycyrrhizin were performed by the high performance liquid chromatography (HPLC)/electrospray ionization (ESI)- mass spectrometry (MS). For the HPLC experiment, a $C_{18}$($3.9{\times}300mm$, $10{\mu}m$) column was used and the mobile phase was acetic acid/$H_2O$ (1:10):acetonitrile=3:2 with a flow rate of 0.8 ml/min. The effluent was splitted into the ratio of 50:1 and went into the ESI-MS. Three to six impurities were found and informed of the identification of the structure of the impurities by ESI-MS. And the structures of impurities were suggested to a hydroxy-glycyrrhizin which is added with hydroxy group (-OH) in the glycyrrhetic acid moiety and a reduced-glycyrrhizin which the position of 12 of the glycyrrhetic acid moiety is reduced. The purities of the standard materials were about 90%.

• Journal of the Korean Society of International Agriculture
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• v.23 no.5
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• pp.531-536
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• 2011

This study was conducted to investigate the effects of cultural soil textures on growth and quality of Glycyrrhiza uralensis Fischer from 2009 to 2010. The obtained results from this study were summarized as follows; The growth of stem and leaf were superior to one year old G. uralensis, and surface runner and root growth tended to be better in 2 years old G. uralensis. The weight of stem and leaf were heavy in sandy loam, and plant height, branches, stem diameter in sandy clay loam were better than other soil texture. The growth characteristics, such as length, number and weight of surface runner, was better in order of sandy clay loam > sandy loam > loamy sand. The length of main and lateral root was longer in loamy sand soil than other treatments, and the diameter of main and lateral root was more thicker in sandy loam than others. The number of lateral root was higher in the sandy loam than other treatments. The yield of main and lateral root was in order of sandy loam > sandy clay loam > loamy sand soil. Marketable root yield of one year old and two year old G. uralensis were increased 57% and 71% in sandy loam compare to a loamy sand as 204 kg/10 a, respectively. The content of glycyrrhizinic acid was the hightest as 1.62% in sandy clay loam soil in one year old, and as 1.58% in sandy loam soil in two years old of G. uralensis, respectively.