• 한국식품영양과학회지
• /
• 제19권4호
• /
• pp.349-355
• /
• 1990

Actomyosine prepared from Yellowtail fish(seriola quinqueradiata) were stored at $0^{\circ}C$(ice-cooling) -3.5$^{\circ}C$(partial freeaing) and -2$0^{\circ}C$(freezing) Another actomyosin samples were prepared from the fish previously stored at the temperatures for a week as the maximum .Remaining activity of {{{{ {Ca }^{2+ } }}}}-and {{{{ {Mg }^{2+ } }}}}- dependent adenosine triphosphatase(ATPase) activity was measured fronm the actomyosin preparations. Specific activity of {{{{ {Mg }^{2+ } }}}}-ATPase of actomy-osin before storagew was 0.253$\mu$ mole pi/min/mg of protein and it was 1.5 times higher than that of {{{{ {Ca }^{2+ } }}}} -ATPase. The enzyme activities were markedly decreased during early period of storage. However no significant differences in the enzyme activity were revealed among the samples stored at different temperature. The enzyme of actomyosin prepared from the fish previously stored at the temperatures for a week revealed an acitivity of 2-3 times higher than that of freezing. Apparent denaturation constant of {{{{ {Mg }^{2+ } }}}} -ATPase of actomyosin was between 0.810-1.139 per day and it was about 1.5 times hgiher than that of {{{{ {Ca }^{2+ } }}}} -ATPase. But the constant of {{{{ {Mg }^{2+ } }}}} ATPase of actomyosin extracted from the fist stored for a week at each temperature was between 0.176-0.356 per day. This constant was 4 times higher than that of {{{{ {Ca }^{2+ } }}}}- ATPase in frozen stored fish. It was presumed from these results that denaturation of ATPase is largely accorded to the structural changes of actomyosin.

• Archives of Plastic Surgery
• /
• 제34권3호
• /
• pp.392-394
• /
• 2007

Purpose: Inflammatory myofibroblastic tumor(IMT) is characterized by clonal proliferation of myofibroblastic spindle cells and accompanied by lymphoplasmacytic infiltration. IMT is an uncommon lesion reported to arise in various organs, and is believed to be a reactive inflammatory condition. IMT forms a spectrum of lesions ranging from benign, infection-related lesions to low-grade malignancies, capable of local recurrences and rarely distant metastasis. IMT occurs mostly in the lung, but rarely in the craniofacial region. Methods: A 28-year-old male with painless swelling in the medial canthal area was referred to our department for the last 2 months. A 2cm sized mass was palpated. He was treated with complete local excision. Results: In the study by computerized tomography, a $2.0{\times}0.8{\times}1.0cm$ mass was found in the subcutaneous tissue layer. Grossly, the mass was well-circumscribed, smooth-surfaced, flesh colored, and hard. The tumor was well demarcated from the other tissues. Histopathologic examinations showed bland spindle-shaped cells loosely arranged with scattered lymphoid cells. Immunohistochemical examinations demonstrated a positive reactivity for alpha-SMA and a negative reactivity for desmin and CD34. No recurrence was noted 12 months after surgery. Conclusion: Emphasis is given to complete resection of the tumor for both diagnostic and therapeutic purposes. Further evaluation to find other lesions in different sites should be considered. Continued follow-up is recommended.

• Applied Microscopy
• /
• 제33권4호
• /
• pp.325-333
• /
• 2003

왕거미과 산왕거미(Araneus ventricosus)의 심관과 심근세포의 미세구조적 특성을 주사형 및 투과형 전자현미경으로 관찰하였다. 거미의 심관은 복부마디의 등쪽에 분포되어 있고, 심외막과 심근층으로 구성되어 있었다. 심근층의 근섬유들은 종축을 중심으로 나선상의 배열을 이루고 있었고, 내막이 없이 혈림프에 직접 노출되는 구조를 이루고 있었다. 심관의 외부 표면에서는 3쌍의 심문이 형성되어 있었고, 내강에서는 원형질혈구와 과립혈구, 그리고 편도혈구 등 다양한 유형의 혈구들이 관찰되었다. 특히 심관의 내강을 향해 돌출된 심근돌기 주위에서는 편도혈구들이 대부분을 차지하고 있었다. 편도혈구의 세포질에는 유리 리보조옴이 산재되어 있었고 핵에는 이질염색질과 인이 발달되어 있었으나, 심근층 조직과의 특이한 연접은 관찰되지 않았다. 심근층에는 횡문이 형성되어 있었고, Z-line을 중심으로 근절의 구조를 이룬 근원섬유의 주위에서는 미토콘드리아와 근소포체가 풍부하게 함유되어 있었다. 심관의 배면을 따라 뻗은 신경절의 축삭들이 심근세포와 신경근육간 연접부를 형성하고 있음이 관찰되었다.

• 대한물리의학회지
• /
• 제10권1호
• /
• pp.71-82
• /
• 2015

PURPOSE: The aim of this study is to compare changes in the thickness of the gluteus medius muscle fiber between chronic low back pain(clbp) with gluteus medius weakness and healthy subject. METHODS: Ultrasound imaging was used to measure in the thickness of each fiber of the gluteus medius muscle based on maximal muscle contraction during abduction motion of the hip joint in a healthy group (11 subjects) and a chronic CLBP group (21 subjects). An independent t-test was performed to analyze the difference of thickness in each fiber of the gluteus medius muscle and the rate of changes in the fibers in the two groups. RESULTS: The fiber thickness changes in the gluteus medius muscle were significantly lower for the posterior fiber in the CLBP group compared to the healthy group (p<0.01). The changes in rate of difference of thickness the posterior part of the gluteus medius muscle was significantly lower in the CLBP group than in the healthy group (p<0.05). CONCLUSION: The findings of this study CLBP patients with weakness of the gluteus medius muscle that lower for the posterior fiber's difference of thickness and rate of change in the CLBP group compared to the healthy group. Indicate that rehabilitation of CLBP patients with weakness of the gluteus medius muscle should consider the functions of posterior fiber of the gluteus medius muscle.

• 한국식품과학회지
• /
• 제20권3호
• /
• pp.371-379
• /
• 1988

한류성 어류(연어, 명태)와 난류성 어류(상어, 조기)의 근원섬유 단백질의의 생화학적인 특성을 규명하기 위하여 각각으로부터 actomyosin과 myofibril을 조제하여 생물활성 및 SDS-polyacrylamide gel 전기영동상을 조사하였으며, 또한 어류의 환경온도에 대한 적응성을 알아보기 위하여 생물활성의 온도 의존성을 조사하였다. 각 어류의 myofibril의 SDS-polyacrylamide gel 전기영동상으로부터 SDS-PAGE pattern은 어종간에 차이를 보였으며 또한 myofibril과 actomyosin의 생물활성과 activity-ph curve도 어종간에 차이를 나타내었다. 한류성 어류인 명태의 근원섬유 단백질의 ATPase의 활성은 환경온도$(5^{\circ}C-10^{\circ}C)$ 부근에서 상어보다 높은 활성을 나타내었다.

• 한국어병학회지
• /
• 제25권2호
• /
• pp.85-93
• /
• 2012

노지 양식장에서 비단잉어와 함께 사육중인 참붕어의 미병부, 체측근, 복부 및 아가미 뚜껑 내부에 황백색의 cyst내의 metacercaria를 탈낭시켜 형태학적 및 분자생물학적으로 조사한 결과 Clinostomum complanatum으로 동정되었다. 병리조직학적으로는 상피세포의 박리와 괴사 및 cyst주변의 근섬유의 위축이외에 다른 조직의 변화는 발견되지 않았다.

• 대한세포병리학회지
• /
• 제17권1호
• /
• pp.63-68
• /
• 2006

Inflammatory myofibroblastic tumor (IMT), normally referred to as inflammatory pseudotumor, is a fairly rare condition. Fine needle aspiration cytology (FNAC) of IMT has only rarely been reported. Here, we describe one such case of pulmonary inflammatory myofibroblastic tumor. A 30-year-old man presented with a 2.8cm-sized mass in his lung. Chest CT revealed a well defined, poorly enhancing mass. FNAC showed some fascicular or swirled clusters of spindle cells, admixed with occasional inflammatory cells and foamy histiocytes. The majority of the tumor cells evidenced bland, elongated nuclei, but infrequent pleomorphic nuclei. Some of the tumor cells evidenced nuclear grooves and intranuclear inclusions. Although the cytological differentiation of IMT from malignant lesions is not immensely problematic, due to the general paucity of cytological and nuclear atypia, a definite cytological diagnosis of IMT cannot be rendered simply by FNAC. Therefore, a diagnosis of IMT may be suggested via exclusive diagnosis.

• Advances in pediatric surgery
• /
• 제14권2호
• /
• pp.153-163
• /
• 2008

Inflammatory myofibroblastic tumor (IMT) is a rare reactive lesion characterized by the feature of myofibroblasts and a mixed inflammatory infiltrate that rarely undergoes malignant transformation. Extrapulmonary IMTs in children have been described involving the mesentery, omentum, retroperitoneum, abdominal soft tissues, liver, bladder, mediastinum, head and neck, extremity, appendix, and kidney. Medical records of children treated with abdominal IMT between 1985 and 2005 were reviewed retrospectively. Seven children were treated for IMT with the mean age of 3 y 2 m (range, 1 y 1 m to 14 y). Tumors were located in transverse mesocolon (n=2), omentum (n=1), porta hepatis (n=2), complex site (antrum, duodenum, common bile duct, porta hepatis) (n=2). The symptoms included abdominal mass, fever, jaundice, abdominal pain and anemia. The masses were excised totally in transverse mesocolon, omentum IMT and there is no evidence of recurrence (follow-up periods: 6 y 8 m, 8 y 9 m, 4 y 10 m). In porta hepatis IMT, liver transplantations were performed and there is no evidence of recurrence (follow period: 6 y 8 m, 8 y 7 m). In one case of complex site IMT, partial excision of mass was performed and he still survived with no change of the residual tumor during follow-up period. The other one of complex site IMT denied further treatment after the biopsy. In conclusion, complete surgical excision including liver transplantation and close follow-up are mandatory for the abdominal IMT in child.

• 한국식품영양과학회지
• /
• 제21권6호
• /
• pp.712-718
• /
• 1992

산 강직근과 알카리 강직근의 사후 자장중 물리, 화학적 변화를 비교 검토하였다. 근섬유의 소편화도는 산강직근이 알카리 강직근에 비하여 저장 3일째부터 월등히 높았다. $Mg^{2+}$-ATPase 활성은 알카리 강직근이 저장 전기간 동안 산 강직근 보다 높았으며, 산 강직근은 저장 1일째, 알카리 강직근은 저장 3일째에 가장 높았다. $Ca^{2+}$-ATPase 활성에 있어서 산 강직근과 알카리 강직근 모두 전 저장기간 동안 약간 증가하는 경향이었으나 산 강직근은 알카리 강직근 보다 전기간 동안 활성도가 낮았다. Actomyosin의 용해성이나 myofibril의 전기영동상은 강직 형태에 따른 유의한 차이가 없었다.

• 한국패류학회지
• /
• 제31권2호
• /
• pp.151-158
• /
• 2015

본 연구는 구리에 노출된 꼬막, Tegillarca granosa의 생존율, 호흡율 및 기관계의 반응을 알아보고자 하였다. 실험기간은 4주였으며, 실험구는 대조구와 구리 노출구 3개 (0.125, 0.250 and 0.500 mg/L) 였다. 실험 결과 구리는 꼬막의 생존율과 호흡률의 저하 및 기관계의 조직학적 변성을 유도하는 것이 확인되었다. 노출 종말점에서 0.500 mg/L의 구리 노출구에서 사망률은 66.7%였으며, 호흡률은 대조구에 비해 18.2%로 감소하였다. 기관계의 조직학적 분석 결과, 외투막 상피층과 결합조직층의 변성, 아가미 새엽 상피세포의 위축과 측면섬모의 소실, 발에서는 상피층의 붕괴, 점액의 산성화 및 혈림프동의 확장과 근섬유 다발의 변성을 나타냈다. 소화맹낭의 소화선세관에서는 호염기성세포와 상피세포의 위축 및 붕괴가 확인되었다.