• Korean Journal of Plant Tissue Culture
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• v.25 no.6
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• pp.507-514
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• 1998

The effects of media and elicitors on betalain, phytolaccoside G and D2 production were examined in the hairy roots of Phytolacca esculenta van Houtte induced by Agrobacterium tumefaciens $A_4$T. Phytolaccoside G and D2 from Phytolacca hairy roots PEH2 were identified by TLC, HPLC, IR, Mass, $^1$H-NMR, and ^(13)C-NMR. Among the culture media tested, SH medium was the best for hairy root growth of hairy roots. White medium was the most suitable medium for betalain production, while MS medium was for phytolaccoside G and D2 production. Although the growth of hairy roots was supped by light (1,000 Lux), the production of betalain, phytolaccoside G and D2 was enhanced by the same light treatment. Addition of elicitors such as NaF, chitosan, and yeast extract to the culture medium increased the content of betalain, phytolaccoside G and D2, suggesting the importance of culture condition for the production of those componds.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2010.04a
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• pp.537-540
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• 2010

본 논문에서는 보의 주철근을 대상으로 건물정보모델링(BIM, Building Information Modeling)통합 설계 시스템 상에서 배근 설계 및 철근 형상화 알고리즘을 구축하여 자동배근 시스템(ARPM, Automatic Reinforcement Placing Module)을 개발하는데 목적이 있다. 구조분야에서의 BIM 프로세스는 정보호환체계를 구축하는 과정에 있으며, 철골 구조 프로세스의 경우 표준 호환 포맷을 이용하여 원활한 정보 호환 체계를 유지하고 있다. 하지만, 철근 콘크리트의 BIM 통합 설계 시스템은 철근 배근정보의 생성과 호환이 원활하지 않아 표준 정보 호환 체계가 구축되지 않은 실정이다. 기존 2차원 기반 프로세스에서는 철근 배근 설계에 있어 표준화된 기준에 따른 배근이 아닌 관행이나 일률적인 배근 지침에 따라 배근 상세를 정하고 있고, 2차원 배근 설계 결과만 제시하고 있어 상호 호환 가능한 철근 배근 정보데이터가 생성되지 않는다. 철근 콘크리트 구조에서의 철근 배근 정보를 생성하고 BIM 통합 구조 설계시스템에서의 정보 호환성을 확보하기 위해, 보의 주철근을 대상으로 구조 해석 데이터베이스와 통합 설계 플랫폼 간의 호환 시스템을 생성하고, 콘크리트학회 콘크리트 구조설계기준에 따른 배근 설계 및 철근 형상화 알고리즘을 구축하여 자동배근시스템(ARPM)을 개발하는데 목적이 있다.

• Korean Journal of Plant Tissue Culture
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• v.21 no.6
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• pp.327-332
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• 1994

The effect of methylglyoxal-bis (guanylhydrazone) (MGBG) and ethylene synthesis inhibitors on adventitious root formation from soybean cotyledon in relation to ethylene production and endogenous polyamine content was investigated. Cotyledon explants cultured on rooting medium formed numerous adventitious rook on the cut surfaces after 2 weeks of culture. However when cultured on rooting medium supplemented with MGBG, the root formation was strongly inhibited, its inhibitory effect was reserved when cultured on medium with MGBG + spermine, MGBG + CoCl$_2$ and MGBG + spermine+CoC1$_2$. A slight reversion of the rooting inhibition was observed in 10$^{-3}$ M MGBG +10$^{-5}$ M spermine treatment, whereas it caused a significant effect in 10$^{-3}$ M MGBG +10$^{-4}$ M treatment .Ethylene production and endogenous polymine content was investgated in 10$^{-3}$ M MGBG , 10$^{-3}$ M MGBG +10$^{-5}$ Mspermine, 10$^{-3}$ M MGBG +10$^{-4}$ M CoCl$_2$and 10$^{-3}$ M MGBG +10$^{-5}$ M spermine +10$^{-4}$ M CoCl$_2$treatments. Ethylene production highest in 10$^{-3}$ M MGBG +10$^{-5}$ M spermine treatment was higher than control. In 10$^{-3}$ M MGBG +10$^{-5}$ M spermine + 10$^{-4}$ M CoCl$_2$ treatment, ethilene production was lowest, whereas polyamine level was highest.

• Korean Journal of Microbiology
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• v.49 no.3
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• pp.297-300
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• 2013

EPS producing bacteria were enumerated in ginseng root system (rhizosphere soil, rhizoplane, inside of root). EPS producing bacterial density of rhizosphere soil, rhizoplane and inside of root were distributed $9.0{\times}10^6$ CFU/g, $7.0{\times}10^6$ CFU/g, and $1.4{\times}10^3$ CFU/g, respectively. Phylogenetic analysis of the 24 EPS producing isolates based on the 16S rRNA gene sequences, EPS producing isolates from rhizosphere soil (RS) belong to genus Arthrobacter (6 strains) and Rhizobium (1 strain). EPS producing bacteria from rhizoplane (RP) were Arthrobacter (6 strains), Rhodococcus (1 strain) and Pseudomonas (1 strain). EPS producing bacteria from inside of root (IR) were categorized into Rhzobium (6 strains), Bacillus (1 strain), Rhodococcus (1 strain), and Pseudomonas (1 strain). Phylogenetic analysis indicated that Arthrobacter may be a member of representative EPS producing bacteria from ginseng rhizosphere soil and rhizoplane, and Rhizobium is typical EPS producing isolates from inside of ginseng root. The yield of EPS was 10.0 and 4.9 g/L by Rhizobium sp. 1NP2 (KACC 17637) and Arthrobacter sp. 5MP1 (KACC 17636). The purified EPS were analyzed by Bio-LC and glucose, galactose, mannose and glucosamine were detected. The major EPS sugar of these strains was glucose (72.7-84.9%).

• Journal of the KSME
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• v.40 no.12
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• pp.46-49
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• 2000

• Proceedings of the Korean Information Science Society Conference
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• 2000.10a
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• pp.439-441
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• 2000

절차지향 소프트웨어를 객체지향 소프트웨어로의 재공학은 소프트웨어 유지.보수 비용의 절감과 기존의 시스템에 새로운 요구사항을 수용하는 등 많은 장점이 있다. 본 논문은 절차지향 소프트웨어를 재공학하는 과정에서 안전한 지속성(Safe Persistency) 결정문제, 즉 객체의 생성과 생성 주기의 결정 방법을 제안하였다. 본 논문에서는 지속성 결정을 위해 다섯 단계의 모델링 과정을 제시하였으며 모델링 과정을 통해 객체의 정확한 생성과 소멸 시점을 추출하였고 정제 과정을 거침으로써 메시지 전달과 생성/소멸 과정에서 안정성과 일관성을 유지할 수 있도록 하였다.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.485-489
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• 2000

The patterns and contents of ginsenosides were examined in normal root parts and hairy root lines of Panax ginseng C. A. Meyer. Ginsenoside-Rb$_1$, -Rb$_2$, -Rc, -Rd, -Re, -Rf, -Rg$_1$, -Rg$_2$ were detected in normal roots and hairy roots of ginseng. The patterns and contents of ginsenosides in that were very difference each other. The contents of total ginsenoside of hairy root (KGHR-1) was 17.42 mg/g dry wt, it's highest compared to others. Ginsenoside contents of hairy root (KGHR-1) was higher on ginsenoside-Rd, Rg$_1$, KGHR-5 was higher on ginsenoside-Rb$_1$, Rg$_1$, and KGHR-8 was higher on ginsenoside-Rd, Re than others. The contents of total ginsenosides on 6 years old ginseng cultured in the field were high in the order of main root, lateral root and fine roots, and content of ginsenosides in fine roots was 3.2 times higher than that in main root. The ratio of ginsenoside-Rg$_1$to total ginsenosides were about 3.43%, 8.68% and 14.18% respectively on fine root, lateral root and main root, it's very lower than that in hairy roots. It is suggested that specific ginsenosides can be produce in cultures of ginseng hairy roots.

• Physical Therapy Korea
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• v.10 no.4
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• pp.61-68
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• 2003

본 연구의 목적은 운동강도(최대근수축의 10, 30, 50, 70%)에 따라 유발되는 국소적인 근피로가 슬관절의 위치감각 인지에 미치는 영향을 연구함으로써 근력강화의 운동치료적 효과와 운동학습과 관련된 효과를 동시에 만족시키는 최적의 운동강도를 제시하는 것이었다. 대상자는 건강한 성인여자 40명이었다. 청각을 통한 위치감각 정보를 제공하는 장치와 원판 각도계가 부착된 등속성 Cybex를 사용하였다. 근피로의 상태를 확인하기 위해서 근전도를 이용하여 주파수 스펙트럼 분석을 실시하였다. 청각되먹임이 주어진 각도와 대상자에 의해 재생되어진 각도의 오차값들과 근피로의 변화를 비교하기 위해 일요인 분산분석을 이용하였다. 오차값들의 평균은 50%의 군에서 가장 작았으며 근피로 또한 70%의 군에서만 크게 생성되었다. 따라서 최대 근 수축력의 50%가 운동치료 시 가장 효과적인 운동강도임을 알 수 있었다.

• Korean Journal of Microbiology
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• v.46 no.3
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• pp.278-285
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• 2010

We examined the distribution of exopolysaccharide (EPS) producing bacteria population in rhizosphere soils of domestic medicinal herbs; Angelica sinensis, Atractytodes japonica, Achyranthes japonica, Anemarrhena asphodeloides, and Astragalus membranaceus. Fifty-six percent of the total isolates from rhizosphere soil of Angelica sinensis were EPS producing bacteria, suggesting the dominance of EPS producing bacteria in rhizosphere soil of Angelica sinensis. EPS producing bacteria were enumerated in root system (rhizosphere soil, rhizoplane, inside of root) of Angelica sinensis. Bacterial density of rhizosphere soil, rhizoplane, and inside of root were distributed $9.0{\times}10^6CFU/g{\cdot}soil$, $7.0{\times}10^6CFU/g{\cdot}soil$, and $1.4{\times}10^3CFU/g{\cdot}soil$, respectively. EPS producing bacteria from rhizosphere soil were categorized into five major phylogenetic groups: Alphaproteobacteria (4 strains), Betaproteobacteria (6 strains), Firmicutes (2 strains), Actinobacteria (3 strains), and Bacteroidetes (1 strain) subdivisions. Also, the EPS producing isolates from rhizoplane were distributed as 7 strains in Alphaproteobacteria, 3 strains in Betaproteobacteria, 2 strains in Actinobacteria, 3 strains in Bacteroidetes, and 1 strain in Acidobacteria subdivisions. All of the EPS producing bacteria inside of root belong to genus Chitinophaga. Burkholderia caribiensis DR14, Terriglobus sp. DRP35, and Rhizobium hainanense SAP110 were selected in 112 EPS producing bacteria. These appeared to have produced high levels of exopolysaccharide 6,555 mpa.s, 3,275 mpa.s, and 1,873 mpa.s, respectively. The purified EPS was analyzed Bio-LC. As neutral sugars, glucose, galactose, mannose were detected and as amino sugars, galactosamine and glucosamine were detected. Especilally, analysis of Bio-LC showed that Rhizobium hainanense SAP110 produced glucose (60~89%) and glucosamine (8.5%) as major neutral sugar and amino sugar, respectively.

• Korean Journal of Plant Tissue Culture
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• v.25 no.1
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• pp.21-25
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• 1998

The effects of sucrose concentration and some absorbents on growth and tropane alkaloid production in hairy root cultures of Scopolia parviflora were investigated. The maximum effect on growth and tropane alkaloid production in hairy root clone SP11 was obtained for 1/2 B5 medium containing 5% sucrose. The production pattern of tropane alkaloid in hairy roots was some different from that of rhizome of mother plant, particulary showing high littorine contents, which was not found in ordinary roots. Among absorbents examined, charcoal 0.01% and XAD-II 1% made a slight growth promotion effect, whereas the other concentration of charcoal, XAD-II and absorbents (amberlite and chitosan) showed inhibition or no significant effect. The addition of hydroxyapatite enhanced the production of tropane alkaloids significantly than control cultures.