• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.72-76
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• 2002

This study was carried out to determine an effective mechanical harvester for rhizomes of Rehmannia glutinosa Libosch. Labor-saving efficiency showed 69∼76% by using tillage operations with power tiller, digger attached to power tiller, and digger attached to tractor compared with manual harvest. Loss percentage of rhizomes by mechanical harvesters was 3.1∼ 9.3% higher, and fresh rhizome yield was 2∼6％ lower than that by manual harvest, respectively. Although it showed higher loss percentage of rhizomes and lower fresh rhizome yield compared with manual harvest, mechanical harvest using digger attached to power tiller or digger attached to tractor was effective in labor-saving for rhizomes of Rehmannia glutinosa Libosch.

• Applied Biological Chemistry
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• v.37 no.1
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• pp.1-8
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• 1994

This study was carried out to investigate the changes in volatile constituents concerning with the flavor of the green ginger (Zingiber officinale Roscoe) during storage in underground pit ($15^{\circ}C$, RH 95%). And the constituents of essential oil of etiolated shoots formed on the mother rhizomes during the five months storage in the dark under same conditions were compared with those of mother rhizomes. The essential oils of Korean domestic ginger (Bong-dong cultivar) were isolated by simultaneous steam distillation and extraction method (SDE). Then the compositions of the essential oils were analysed by GC and GC-MS spectrometry. The major compounds of essential oil from the fresh rhizomes were zingiberene, $citronellol+{\beta}-sesquiphellandrene$, ${\beta}-phellandrene$, camphene, geranial, ${\gamma}-bisabolene$, ar-curcumene+geranyl acetate, ${\alpha}-pinene$, ${\beta}-gurjunene$, limonene and neral. The content of monoterpene hydrocarbons increased with a concomitant lowering in the amounts of sesquiterpene hrdrocarbons and oxygenated sesquiterpenes during storage of rhizomes although contents of the oxygenated monoterpens changed little or slightly during the storage. During the storage the content of such monoterpenes as camphene, ${\beta}-phellandrene$ and citral (neral and geranial) increased whereas the content of such sesquiterpenes as zingiberene and $citronellol+{\beta}-sesquiphellandrene$ decreased. The composition of shoot oil differed from that of mother rhizome oil in having higher content of terpene hydrocarbons and also in the higher content of bornyl acetate, ${\beta}-gurjunene$ and ar-curcumene+geranyl acetate and lower in citral (neral and geranial).

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.266-272
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• 2018

A Polygonatum stenophyllum Maxim. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of P. stenophyllum through plant regeneration from rhizome (1-year, 3-years, and 5-years) explant-derived calli. The rhizome segments were cultured in Murashige and Skoog (MS) medium supplemented with varying concentrations of 2,4-D (0, 0.5, 1.0, $1.5mg{\cdot}L^{-1}$) for callus induction. In media supplemented with $0.5mg{\cdot}L^{-1}$ of 2,4-D, 87% of 3-years rhizome produced callus. Subsequently, the callus was transferred to 1/2MS medium supplemented with various concentrations of IAA, IBA, NAA, and 2,4-D (0, 0.1, 0.5 and $1.0mg{\cdot}L^{-1}$) for adventitious shoot formation. The highest percentage of adventitious shoot induction (57%) was observed in 1/2MS medium containing $0.5mg{\cdot}L^{-1}$ of NAA. Elongation of the adventitious shoot was achieved in 1/2MS medium supplemented with $0.1mg{\cdot}L^{-1}$ of BA. Rooting was achieved in 1/2MS medium without any hormones. It is hypothesized that the stated in vitro propagation protocol will be useful for conservation and mass propagation of the endangered Polygonatum stenophyllum Maxim. for bioresources.

• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.21-29
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• 2005

This study was done to obtain their morphological traits to analyse genetic diversity and intraspecific relationship of 47 Polygonatum species collected from Gyeongnam province. Plant height was the highest in P. thunbergii but the shortest in P. involucratum. Growth habit and its colors were classified to 3 groups, respectively. Leaf shapes were sorted to 5 groups including lanceolate with petiole or none, petiole colors were done to 3 groups including a species having dark green leaves of purple colored margin. Flower shapes were divided as 3 groups of urceolate, tubular and gourd shapes, and its colors were white, greenish white and light green, especially light green in a species with gourd shape. Filament shapes were two types of flatness and cylinder. Peduncle color and bract attached below it showed 4 types, respectively. Fruit shapes were sorted to 3 groups. In 100-fruit weights P. ordoratum var. pluriflorum showed the greatest but P. involucratum did the least. Two species were completely resistant to leaf brightness although 7 species showed less than 7 % infection rates. Rhizome yields ranged from 4.4 g to 94.8 g per plant, showing their significant variation. In correlation analysis between 9 major characters, rhizome yield per plant was positively correlated with plant height, stalk diameter, leaf number, leaf length and width, and rhizome diameter but leaf brightness was negatively done with plant height, stalk diameter, leaf number and length, 100-seed weight, rhizome yield per plan and rhizome diameter.

• Korean Journal of Weed Science
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• v.16 no.4
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• pp.309-316
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• 1996

In this study, germination characteristics and herbicidal response of green kyllinga(Kyllinga brevifolia var. leiolepsis H.) were investigated. The storage method desirable for a rapid dormancy release was to keep the seed under low temp. and wetting condition for one to two months, or high temp($40^{\circ}C$) and drying condition for three months. The dormancy of rhizome was hardly observed. The optimum temperature for germination of seed and rhizome was around $30^{\circ}C$ and 16-$20^{\circ}C$, repectively. The germination of dormancy-breaked seed was completely dependent on light. Shoot emergence ratio(%) was decreased with increase of planting depth ; for example, only 18% of rhizome segments planted in the depth of 4cm under soil surface emerged above soil surface. Flooding at earlier growth stage resulted in significant decrease in shoot emergence as well as in dry weight. The germinablity of rhizome was almost lost as a decreased in fresh weight reached to 50%. Usually, green kyllinga was sensitive to herbicides such as bentazone, bensulfuron and benfuresate etc. which were known to be effective in Cyperaceae weeds, indicating that green kyllinga can be used as a representative plant in the screening of herbicides for Cyperus weeds.

• Research in Plant Disease
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• v.21 no.3
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• pp.215-221
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• 2015

This study was conducted to control ginger rhizome rot treated with the combined treatment, the hairy vetch, carbonized rice husk and eggshell calcium in organic ginger farm. Early symptoms of leaf yellowing and plant wilt began in the chemical fertilizer treatment on July 1. Ginger rhizome rot was more progressed on October 2, and stem browning and dead plant showed a high disease incidence with from 36.7% to 43.0%. On the other hand, the combined treatment did not occur at all until July 1 and delayed the disease incidence to October 2. It showed a low disease incidence of 1.3% to 1.7%. In the combined treatment, the content of soil Na, Fe, Cu was decreased and organic matter was increased twice with 31.6% than previous. Population density of Pythium sp. is lower in the combined treatment ($0.3-2.0{\times}10^3cfu/g$ than the chemical fertilizer treatments ($12.0-12.3{\times}10^3cfu/g$). The combined treatment, hairy vetch, carbonized rice husk and the eggshell calcium is able to control the ginger rhizome rot in organically cultivated ginger field.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.183-188
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• 2004

This study was conducted to investigate the effects on the growth and yield by the different seeding methods and cultivating root weight in Atractylodes macrocephata Koidz. Seeding distances were different such as 5${\times}$5 cm, 5${\times}$10 cm, 10${\times}$10 cm, 10${\times}$15 cm by the hand seeder and 15 cm seeding in drill. Emergence date, plant height were not significantly changed with seeding space, but the number of leaf, fresh weight of above-ground part and fresh weight of root were increased in the sparse seeding compared with the dense seeding. The highest fresh root yield was 1,012 kg/10 a at the 5${\times}$5 cm. Roots yield was increased in the sparse seeding compared with the dense seeding, but the highest number of roots above 16 g yield was observed at the 10${\times}$10 cm seeding distance. Emergence date was faster 1∼3 day root weight above 6 g than that root weight 5 g. The plant height, number of stem and fresh weight of above-ground part were more increased as the root weight was heavier. The growth of underground part were more increased as the root weight was heavier, yield was increased about 27％ to 112％ compared with root weight above 6 g than that 134.6 kg/10 a with root 5 g.

• Korean Journal of Plant Resources
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• v.22 no.1
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• pp.42-48
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• 2009

This study was conducted to examine the difference in yield and size of enlarged rhizome according to the kinds of native white lotuses. Four white lotuses (Nelumbo nucifera) were collected from 4 different sites, Jeonbuk Jeonju ('Garam'), Jeonnam Muan ('Hoesan'), Chungnam Buyeo ('Ihnchuisa'), and Chungnam Cheongyang('Cheongyang'). Each enlarged rhizome was planted in an area of 3.3 $m^2$ on 10. April, 2007 and digged out on 19. February, 2008. The yield of enlarged rhizome was in the order of Hoesan (3.0 kg/$m^2$), Cheongyang (2.5 kg/$m^2$), Garam (2.0 kg/$m^2$) and Ihnchuisa (1.0 kg/$m^2$). The number of enlarged rhizomes was in the order of Ihnchuisa (4.9/$m^2$), Garam (4.3/$m^2$), Hoesan (4.2/$m^2$) and Cheongyang (4.1/$m^2$). Total number of nodes in the enlarged rhizomes were much more in the order of Hoesan (17.0/$m^2$), Ihnchuisa (16.0/$m^2$), Cheongyang (15.3/$m^2$) and Garam (14.1/$m^2$). Component ratio of enlarged rhizome length below 50cm for Ihnchuisa, Garam, Hoesan and Cheongyang were 76.3%, 53.0%, 38.2% and 40.3% respectively. Native white lotus had a longer enlarged rhizome in the first node than the other nodes, and their enlarged rhizome length and width became thin. And it became thick and short from the second node of enlarged rhizome. Accordingly the amount of enlarged rhizomes 'Hoesan' was extremely much than others.

• Korean Journal of Medicinal Crop Science
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• v.7 no.1
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• pp.31-36
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• 1999

This study was carried out to investigate the effect of sulfuric fertilizers on allylisothiocyanate(allylNCS) content of upland Wasabia japonica Matsum cultivated in heating condition in the winter season. The rhizome's growth characters following soil application of sulfur dust were not significantly different compared to control, though its soil acidity and available sulfur content was higher than other treatments. Total sulfur content of plant was increased by soil application as compared to foliar application and allylNCS content of rhizome increased up to 22.8% in the sulfur dust treatment. The result showed that soil application was effective for supplying $SO_4^{-2}$ in upland Wasabia japonica Matsum when cultivated in heating condition. In the analysis of correlation coefficients between growth and quality characteristics, allylNCS content of rhizome showed the significantly positive correlation with total sulfur content of shoot, rhizome and allylNCS content of shoot.

• Korean Journal of Plant Resources
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• v.26 no.4
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• pp.411-416
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• 2013

This study was conducted not only to analyze ${\alpha}$-glucosidase inhibition activity with fronds and rhizomes of nine Pteridophyte species, but also to select the plant materials suitable for natural ${\alpha}$-glucosidase inhibitor. Harvested rhizomes and fronds were washed, freeze-dried and grinded. After conducting ultrasonification extraction for 30 minutes in ultrasonic water tank with 100% methanol solvent, and vacuum filtration, ${\alpha}$-glucosidase inhibition activity was measured. Acarbose was used as the positive control. After mixing $100{\mu}L$ of 0.7 unit ${\alpha}$-glucosidase enzyme solution into $50{\mu}L$ of extract and reacting them at $37^{\circ}C$ for 10 minutes, $50{\mu}L$ of 1.5 mM ${\rho}$-NPG solution was taken and reacted at $37^{\circ}C$ for 20 minutes. The reaction was stopped with 1 mL of 1 M $Na_2CO_3$ and absorbance was measured in 405 nm. With the regression analysis, the content of solubility solids (the value of $IC_{50}$) which can inhibit 50% of 0.7 unit ${\alpha}$-glucosidase solution's activity was investigated. The frond ($IC_{50}=14.00{\sim}913.33{\mu}g{\cdot}mL^{-1}$) and rhizome extracts ($IC_{50}=12.93{\sim}205.84{\mu}g{\cdot}mL^{-1}$) of nine Pteridophyte species showed higher ${\alpha}$-glucosidase inhibition activity in comparison with acarbose ($IC_{50}=1413.70{\mu}g{\cdot}mL^{-1}$). The extracts of fronds and rhizomes showed higher value than acarbose by 1.55~100.98 and 6.87~109.33 times each. Especially, ${\alpha}$-glucosidase inhibition activities of Pyrrosia lingua in fronds and Osmunda cinnamomea var. fokiensis in rhizomes were the highest. The necessary biomass of fronds and rhizomes for inhibiting 50% of ${\alpha}$-glucosidase activity showed the lowest value, 0.35, 0.27 mg each, in O. cinnamomea var. fokiensis. $IC_{50}$ value of P. lingua was the highest among fronds of nine Pteridophyte species, but content of soluble solids was 2.4 times less than O. cinnamomea var. fokiensis. So frond of O. cinnamomea var. fokiensis is more economic in comparison with P. lingua. As the result of this study, O. cinnamomea var. fokiensis showed high ${\alpha}$-glucosidase inhibition activity even with small biomass. Therefore it was considered to be high-valued economic material as natural ${\alpha}$-glucosidase inhibitor.