• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.spc
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• pp.96-102
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• 2008

This study was carried out to examine agronomic characteristics and seed qualities of 827 Korean soybean landraces according to the collecting areas. Agronomic characteristics and seed qualities was examined according to the localities by weather characteristics. Days to flowering was the longest (mean 70.9 days) in the northwest seashore, and the most short in the south inland (mean 63.8 days). Growth days was the longest in the Gyeongbuk inland (mean 136.0 days), and the most short in the mountain-high (mean 132.8 days). The stem height was the most short in the north-west seashore (mean 81.3 cm), and in the south seashore (mean 58.8 cm). The seed weight of 100 seeds was the lightest in the north-west seashore (mean 17.1 g), and the heaviest in the south-west seashore (mean 21.5 g). The crude protein contents was the lowest in the north-west seashore (mean 40.1%), the highest in the south seashore (mean 42.8%), and vice versa in the crude oil. The contents of sucrose and oligo-saccharide was the highest 5.8% and 8.6%, respectively, in the mountain-high, and the lowest 4.8% and 7.6% in the Gyeongbuk inland and south seashore, respectively. Among the iso-flavone contests, the contents of genistein and total was the highest, $635.6\;{\mu}g/g$ and $1258.1\;{\mu}g/g$, respectively, in the mountain inland, and the lowest $319.2\;{\mu}g/g$ and $835.3\;{\mu}g/g$ in the south-west seashore. The contents of glycitein was the highest $124.3\;{\mu}g/g$ in the south seashore.

• Korean Journal of Animal Reproduction
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• v.25 no.2
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• pp.101-111
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• 2001

The present study was conducted to develop an in vitro culture system that would support bovine follicle growth from preantral to antral stage, oocyte maturation, fertilization, and embryonic development. Bovine preantral follicles (150$\pm$1.2 ${\mu}{\textrm}{m}$) surrounded by theca cell were isolated ezymatically and mechanically from ovarian cortical slides in Leibovitz L-l5 medium containing 1 mg/$m\ell$ collagenase and 0.2 mg/$m\ell$ DNase I and cultured for 25 days in the presence of different concentrations of bovine FSH and LH in $\alpha$MEM medium. The survival and growth rates of follicles cultured in the presence of FSH (10~150 ng/$m\ell$) were significantly higher than those of control group (P ＜ 0.001), but no significant differences were observed in survival and growth rates of follicles between the LH treatment groups (1~125 ng/$m\ell$) and the control. The survival (40%) and growth (244 $\pm$ 0.5 $\mu\textrm{g}$) of follicles cultured with FSH (90 ng/$m\ell$) and LH (25 ng/$m\ell$) were higher than those of control (25%, 160 $\pm$1.0 $\mu\textrm{g}$). Finally, 50% percent of healthy antral follicles were obtained, and almost 60% of them has complete meiotic division with 1st polar body (18.1%) and 10.0% have developed to the cleaved embryo and blastocyst stage. These results suggest that bovine preantral follicle with intact theca cell can grow to the antral stage using these culture conditions, and that oocytes from in vitro-matured bovine preantral follicle may acquire meiotic competence and can undergo fertilization and development.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.40 no.1
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• pp.9-16
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• 2004

Parapenaeopsis tenella occurs widely on the south and west coasts of Korea. Sex ratio showed seasonal variations, with a mean value of 48.6% for the females. The species produces one cohort a year, with the ovaries ripening from July to August. Insemination took place from July to August, as more than half of the females sampled in the study over 14 mm CL were inseminated. Gonadosomatic index (GSI) reached the maximum between July and August. The smallest mature female was 11 mm CL. Size at 50% sexual maturity ($CL_{50}$), determined from both mature females and inseminated females, was 12.53 mm and 12.28 mm CL, respectively. The life span of females appeared to be 14-15 months according to size frequency distributions, while that of the male was 13-14 months. Population growth was estimated by the modified von Bertalanffy growth function incorporating seasonal variation in growth. Based on the growth parameters (K = 1.22 $yr^{-1}$ and $L_{\infty}$ = 21.99 mm CL for females, and K = 2.00 $yr^{-1}$ and $L_{\infty}$ = 15.00 mm CL for males) growth curves showed that females grew faster and reached a larger size at age than males.

• Journal of Yeungnam Medical Science
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• v.18 no.1
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• pp.94-100
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• 2001

Background: It is doubtful that aging causes deteriorated glucose metabolism and insulin resistance of skeletal muscle. Some researchers had different results about it. So we have studied the mechanism responsible for the abnormal glucose tolerance associated with aging in rapidly growing and matured rats. Materials and Methods: Animals were used S.D. rats. Growing rats were 7 weeks old (BW: 160-190 gm) and matured rats were 28 weeks old (BW: 420-525 gm). Results: Fasting blood glucose and plasma insulin levels were significantly elevated in matured rat compared with growing rats. And during oral glucose tolerance test the glucose level was also significantly elevated in matured rats. These results confirmed an insulin resistant state of aging. Insulin levels at 30 minutes of oral glucose tolerance test was significantly elevated in growing rat. But at 120 minutes it was maintained at higher level in matured rats than in growing rats. It suggested the possibility of increased insulin secretion by initial stimulation of beta-cells in growing rats, and increased secretion and decreased catabolic rate of insulin in matured rats. Glucose uptake rate of soleus muscle in matured rats was lower than that of growing rats, but the difference was not statistically significant. The dose(insulin)-responsive(glucose uptake) curve of soleus muscle was only slightly deviated to the right side. Conclusion: Glucose metabolism of rat skeletal muscle was worsened by aging. The data of glucose uptake experiments suggested the possibility of insulin resistance of skeletal muscle in matured rats. but the mechanism of insulin resistance of skeletal muscle need further studies.

• Development and Reproduction
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• v.9 no.2
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• pp.95-103
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• 2005

Reproductive cycle, gonadosomatic index(GSI), egg diameter composition, first sexual maturity, sexually matured length(50% of first sexual maturity), and sex ratio of Amusium japonicum japonicum, were investigated by histological observations and morphometric data. Samples were collected monthly from the subtidal zone of Sogwipo, Jejudo, Korea, for two years. The sun and moon scallop Amusium japonicum japonicum is dioecious. Monthly variation in the GSI showed similar patterns with the reproductive cycle. Ripe oocytes were about $70{\sim}90\;{\mu}m$ in diameter and had thick egg membranes. The spawning period was from November to January, and the main spawning occurred between November and December when the seawater temperature was relatively low. From monthly changes in egg diameter composition, the spawning period was once a year, although the number of spawning frequencies is assumed to occur more than twice during the spawning season. The reproductive cycle of this species could be divided into five successive stages: early active stage(April to June), late active stage(June to September), ripe stage(October to November), spawning stage(November to January), and spent/resting stage(February to April). First sexual maturities in female and male scallops ranging from 85.1 to 90.0mm in shell length were over 50% and they were 100% for scallops over 90.0mm in shell length. In this population, sexually matured shell lengths(50% of rate of group maturity) in females and males were 86.96 and 86.59mm, respectively. The female to male sex ratio among individuals over 85.1mm in shell length was not significantly different from 1:1($X^2=0.18$, p>0.05). No evidence of hermaphrodite was found in histological sections of any scallop examined.

• Korean Journal of Ichthyology
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• v.29 no.2
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• pp.109-116
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• 2017

We investigated maturation and spawning of Miichthys miiuy in the south-western water of Korea, based on samples collected by stow net from August 2015 to December 2016. We analysed monthly changes in maturity stage, gonadosomatic index (GSI), total length (TL) at 50% group maturity. The spawning period was August to October. We estimated the TL at 50% group maturity as 54.8 cm for females and 49.0 cm for males.

• Journal of Software Assessment and Valuation
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• v.16 no.2
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• pp.87-98
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• 2020

Recently, IoT, artificial intelligence, cloud, big data, and mobile fields have converged, leading to a new industrial era called the 4th industrial revolution. This 4th industrial revolution has been expanded to all industry area and Software has been taken as important role in this revolution. Thus Software Safety is the huge factor because Software is highly relevant to human safety in accordance with Software expansion. However this Software Safety has been focused on not organization improvement activities but current design/development, In this paper, to solve this issue, Software Safety Maturity level and relevant Process Area is defined. This study is expected to contribute to systematic software safety activities.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.2
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• pp.163-173
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• 1995

Populations of the common squid, Todarodes pacifirus in the Korean waters were analyzed using the samples taken monthly from February, 1991 to July, 1992. Summer, Autumn and Winter cohorts were arbitrarily established based on frequency distributions of mantle lengths and maturation stages. As cohorts were seperated in mantle length distributions on the basis of accumulated information of size and maturation of T. pacificus, cohorts seperated by the present method were mostly in accord with those by Tanaka's method. But they were not consistent with those by Bhattacharya's and Cassie's methods.

• Korean Journal of Animal Reproduction
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• v.25 no.2
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• pp.181-189
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• 2001

The objective of this study was to evaluate the development of porcine follicular oocytes fertilized by intracytoplasmic sperm injection (ICSI). Cumulus-oocyte-complexes (COCs) were collected by aspiration from follicles of 2~7 mm in diameter from a local slaughterhouse. Oocytes were matured in vitro for 40~44 h, and spermatozoa were prepared by swim-up in the presence or absence of 5 mM dithiothreitol (DTT) and then M II stages of the oocyte were either centrifuged or not centrifuged for the following injection of ooplasm. Injected oocytes were cultured in NCSU 23 medium for 6 to 8 days. The results obtained were as follows: 1. The rates of cleavage and development rates into blastocyst by ICSI were not significantly different between the with (53.0% and 19.7%) or without (48.3% and 23.8%) centrifugation, respectively (P＜0.05). 2. The cleavage and developmental rates to blastocyst after ICSI with or without 5 mM DTT treated-sperm were not significantly different (60.4% vs 16.4% and 45.5% vs 22.2%), respectively (P＜0.05). 3. The cleavage and the developmental rates to btastocyst were not significantly different between the zygotes obtained by IVF (51.8% vs. 22.4%) and ICSI (51.4% vs. 21.6%) (P＜0.05). 4. The number of blastomere in blastocyst stages after IVF or ICSI was not significantly different (46.7$\pm$2.9 and 41.9$\pm$4.6).

• Journal of Embryo Transfer
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• v.21 no.2
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• pp.129-135
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• 2006

This study was conducted to investigate the effect of in vitro maturation (IVM) duration of porcine follicular oocytes on maturation rate, polyspermic rate, and subsequent embryo development. The nuclear maturation rates of oocytes matured for 36, 38, 40, 42 and 44 hr were similar between 68.0, 78.0, 79.5, 73.8 and 81.8% respectively. There was no significant difference in the rates of polyspermy after in vitro feritilization (IVF). The cleavage rate in the group of 36 hr was significantly higher in than that of 40, 44 hr (p<0.05) but not to 38 and 42 hr. The development rate to blastocyst stage was significantly higher in the group of 38 hr (23.1%) than that in the group of 44 hr (15.6%) (p<0.05) but not to 36, 40 and 42 hr. These results suggest that the aged oocytes for 44 hr is not required for the production of bias to cysts derived from porcine IVF embryos.