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LPS Stimulated B Lymphocytes Inhibit the Differentiation of Th1 Lymphocytes (LPS에 의해 자극된 B 림프구에 의한 Th1 림프구 분화 억제)

  • Kim, Ha-Jeong
    • Journal of Life Science
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    • v.25 no.12
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    • pp.1425-1431
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    • 2015
  • The lymphocyte component of the immune system is divided into B lymphocytes and T lymphocytes. B lymphocytes produce antibodies (humoral immunity) via maturation into plasma cells, and T lymphocytes kill other cells or organisms (cellular immunity). A traditional immunological paradigm is that B lymphocyte and T lymphocyte interactions are a one-way phenomenon, with T lymphocytes helping to induce the terminal differentiation of B lymphocytes into immunoglobulin class-switched plasma cells. A deficiency of T lymphocytes was reported to result in defective B lymphocyte function. However, evidence for a reciprocal interaction between B and T lymphocytes is emerging, with B lymphocytes influencing the differentiation and effector function of T lymphocytes. For example, B lymphocytes have been shown to induce direct tolerance of antigen-specific CD8+ T lymphocytes and induce T lymphocytes anergy via transforming growth factor-beta (TGF-β) production. The present study showed that LPS-stimulated B lymphocytes inhibited the differentiation of Th1 lymphocytes by inhibiting the production of interleukin-12 (IL-12) from dendritic cells. An interaction between the B lymphocytes and dendritic cells was not needed for this inhibition, and the B lymphocytes did not alter dendritic cell maturation. B lymphocyte-derived soluble factor (BDSF) suppressed the LPS-induced IL-12p35 transcription in the dendritic cells. Overall, these results point to a novel B lymphocyte- mediated immune suppressive mechanism. The findings cast doubt on the traditional paradigm of immunological interactions involving B lymphocyte and T lymphocyte interactions.

Effect of Feeding Fermented Food Wastes on Consumer Acceptability of Pork Belly (남은 음식물 발효사료 급여가 돈육 삼겹살의 소비자 기호도에 미치는 영향)

  • Park Hong-Yang;Park Keun-Kyu;Jung Young-Chul;Lee Eui-Soo;Yang Si-Yong;Im Byoung-Soon;Kim Cheon-Jei
    • Food Science of Animal Resources
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    • v.24 no.4
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    • pp.386-392
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    • 2004
  • The objective of this experiment was to compare tile effect of feeding the commercial feeds (control) or fermented food waste feeds (FEWF) on consumer's acceptability and preference of pork belly. The bellies from carcasses of gilts and barrows were used. The consumers evaluated raw meat for color, freshness, fat amount (5=too much fat; 1= not enough fat), fat acceptability and overall acceptability on 5-point stale (5=most desirable; 3=moderate; 1=least desirable) and grilled belly for flavor, taste, texture, juiciness and overall acceptability on 5-point scale (5=most desirable; 3=moderate: 1=least desirable). Raw belly of control had higher scores in color than belly fed FFWF. However, there were no differences between treatments for freshness, fat contents, acceptability of fat contents and overall acceptability (p>0.05). There were no differences in any sensory trait between control and FFWF belly after cooking (p>0.05). Mean scores for preference or overall-liking of raw and cooked belly also were not significantly different between two groups (p>0.05). This results mean that feeding FFWF to pork didn't change the sensory Properties of belly meat.

Digestive Enzymatic and Nucleic Acidic Responses of Olive Flounder Paralichthys oilivaceus Larvae Fed Cyclopoid Copepod Paracyclopina nana (기수산 Cyclopoid 요각류 Paracyclopina nana를 섭취한 넙치 Paralichthys olivaceus 자어의 핵산 함량과 소화효소적 반응)

  • Kwon, O-Nam;Lee, Kyun-Woo;Kim, Gun-Up;Park, Heum-Gi
    • Journal of Aquaculture
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    • v.21 no.3
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    • pp.190-195
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    • 2008
  • We investigated the changes in growth, digestive enzymes activities, nucleic acids contents and RNA/DNA ratio of flounder Paralichthys olivaceus larvae (C for Paracyclopina nana, A for Artemia, and M for Mix of C and A) for 14 to 28 DAH. Body length of flounder larvae showed the best in the C trial at 28 DAH. The change of nucleic acids contents showed faster in C and M trials than A trial. And RNA/DNA ratio showed the significantly faster changes in C trial than A trial. High metamorphosis rates were also observed in C and M trial. $\alpha$-amylase activities increased gradually up to 28 DAH in all trials. Total alkaline protease (TAP) activities of A trial showed the highest value to 9 mU/larvae at 26 DAH. But others trials showed lower to $5{\sim}6$ mU/larva than A trial. TAP:$\alpha$-amylase activity ratio did not significantly changed to $0.025{\sim}0.053$ in A trial during the experiments. But, C and M trials tended to gradually decrease from $0.078{\sim}0.083$ (initial) to $0.013{\sim}0.018$ (final). Therefore, it shown the ratio gradually decreased of TAP:$\alpha$-amylase activity, stability of TAP activity, and rapid change of nucleic acids in trials grown positively. Thus, because P. nana could continuously supply the optimal nutrients for flounder larvae, we suggested the supplement of the copepod to an efficient feed of the flounder larvae.

Effects of Supplemental Undaria, Obosan and Wasabi in the Experimental Diets on Growth, Body Composition, Blood Chemistry and Non-specific Immune Response of Juvenile Flounder, Paralichthys olivaceus (배합사료에 미역, 어보산 및 고추냉이 첨가가 넙치 치어의 성장, 체성분, 혈액성상 및 비특이적 면역능에 미치는 효과)

  • 박상언;권문경;이윤호;김경덕;신일식;이상민
    • Journal of Aquaculture
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    • v.16 no.4
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    • pp.210-215
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    • 2003
  • When fed on one of the six isonitrogenous (45%) and isolipic (8%) feed containing 5 or 10% Undaria, 2% wasabi leaf, 2% wasabi stem and 0.5% herb (Obosan) for a period of 8 weeks, 95-98% juvenile flounder survived. Growth, feed efficiency and condition factor of the flounder fed on diet containing 0.5% herb were significantly (p<0.05) higher than those fed on diet supplemented with 10% Undaria. There was no significant (p>0.05) differences in moisture, crude protein, lipid and ash of the flounder receiving the different diets. The flounder fed on diet supplemented with 10% Undaria had the highest moisture but the lowest lipid in liver. Hematological parameters such as red blood cell, hematocrit and hemoglobin and serum constituents such as glucose, total cholesterol and glutamic-oxaloacetic transaminase of the flounder fed on the diets varied but no specific trend became apparent. Lysozyme activity in the serum of the flounder fed on diet supplemented with 5% Undaria and the herb, as well as nitroblue tetrazolium (NBT) reduction of macrophage in the head kidney of the flounder fed on diet containing the herb and 2% wasabi stem were significantly (p<0.05) higher than those receiving control diet. Briefly, the herb supplementation promoted growth and that of Undaria and wasabi stem enhanced non-specific immune response.

Effects of elevated-$CO_2$ concentration and -temperature on the phenological and reproductive responses of Baktae and Seomoktae, Glycine max (L.) Merrill ($CO_2$농도와 온도 상승에 따른 백태와 서목태(Glycine max (L.) Merrill)의 식물계절학 및 번식생태학적 반응)

  • Park, Jae-Hoon;Cho, Kyu-Tae;Kim, Sang-Bum;Jang, Rae-Ha;You, Young-Han
    • Korean Journal of Environment and Ecology
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    • v.28 no.6
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    • pp.634-641
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    • 2014
  • In order to elucidate the ecological and phenological responses of bean under global warming situation, we investigated leafing and flowering phenomena and growth traits of Baktae and Seomoktae in control plot (ambient $CO_2$ concentration and temperature) and warmed plot (elevated $CO_2$ concentration and increased temperature than control) in green house. Average $CO_2$ concentration and temperature were set 540 ppm in treatment and higher $2.2^{\circ}C$ in treatment than control. The appearance of leaf unfolding and the initiation of blooming and fruit maturing in Seomoktae were late in treatment than control. In case of Baktae, the number of total pods and seeds per plant in treatment was fewer than that in control. For Seomoktae, the number of total pods per plant and weight of total seeds per plant were decreased in treatment than in control. Thus, the number of pods and seeds of Baktae declined and the production of Seomoktae was decreased under global warming condition. This result indicates that site selection and crop yield for cultivating of the bean may be changed into new area due to global warming condition.

수세법과 pH 조절법에 의한 닭가슴살 surimi의 품질 특성

  • Park, Gi-Hun;Kim, In-Jin;Ha, Ji-Hui;Gang, Seok-Mo;Kim, Il-Seok;Jin, Sang-Geun
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2005.05a
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    • pp.155-158
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    • 2005
  • 닭가슴살을 이용해서 수리미 제조 시 4회 수세한 대조구, pH 3.0으로 산 처리한 T1, pH 11.0으로 알카리 처리한 T2로 하여 그 품질 특성을 비교한 결과 일반성분에서 수분, 조단백질과 염용성단백질 및 수율은 대조구에 비하여 pH 조절법으로 한 두 구가 높았으며, 조지방은 T2가 다른 두 구에 비하여 높았다. pH, 보수력, 파괴강도, 변형값은 처리 간에 유의적인 차이를 보이지 않았다. 전단가는 T2가 가장 높고, T1, 대조구 순이었다. $L{\ast}$값은 T2가 다른 두 구보다 낮았으며, $a{\ast}$값은 T1이 다른 두 구에 비하여 낮았다. $b{\ast}$값은 대조구가 가장 높고, T2가 가장 낮았다. 조직감에서 파쇄성 및 경도는 처리 간에 유의적인 차이를 보이지 않았으며, 응집성은 T1이 다른 두 구보다 낮았으며, 탄력성과 부착성은 대조구에 비하여 T1과 T2가 높았다. 검성은 대조구와 T1에 비하여 T2가 높았다. 조직감의 전 항목에서 T2 가 가장 높았다. 관능검사 결과 외관과 맛은 T1이 가장 높고, 대조구가 가장 낮았다. 색, 향, 다즙성, 연도 및 전체적인 기호도는 처리 간에 유의적인 차이가 없었다.

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Effects on Quality of 'Fuji' Apple by Dipping of Calcium Solution and MA Packaging (칼슘처리와 MA포장의 저장중 '후지' 사과의 품질에 미치는 영향)

  • Park, Hyung-Woo;Kim, Yoon-Ho
    • 한국포장학회:학술대회논문집
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    • 2006.11a
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    • pp.95-101
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    • 2006
  • 거창사과원예협동조합에서 구입한 사과 600상자를 5% $CaCl_2$ 용액에 각각 15분간 침지하는 방법으로 전처리 후 24시간 건조시키고 무포장구, $25{\mu}m$ 두께의 기능성 MA 필름으로 포장한 포장구와 무처리 무포장구인 대조구로 나누어 $0^{\circ}C$의 저온저장고로 옮겨 7개월 동안 저장하면서 3주 간격으로 품질을 분석하였다. 저장 기간이 증가할수록 중량 감소율은 Control 구보다 $CaCl_2$구와 $CaCl_2+MA$구의 중량 감소율이 월등히 낮았다. 경도변화는 저장 기간이 증가할수록 감소하는 경향을 보였으며, 그 중 $CaCl_2$구는 초기치 0.97 $kg_f$에서 저장 18주에는 0.95 $kg_f$로 초기치에 비해 2%의 가장 적은 변화를 보였다. 산도는 대조구보다 처리구의 산도가 높게 나타났으나 큰 차이는 나타나지 않았다. 가용성 고형분 함량은 저장 기간 모든 실험구에 따른 큰 차이가 없었다. 비타민 C 함량은 저장기간이 지날수록 모든 실험구에서 감소하는 경향을 보였으며, 기호도 조사에서 $CaCl_2$구와 $CaCl_2+MA$구가 대조구보다 유의적으로 높게 나타났다.

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Analysis of Disaster for Common Utility Tunnel & Schemes of Establishing Construction criteria (공동구 재해사례와 건설기준 제정방안)

  • Yoo, Jai-Sung;Lim, Jong-Min
    • Proceedings of the Korean Geotechical Society Conference
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    • 2009.09a
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    • pp.1236-1245
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    • 2009
  • 공동구는 도시생활을 영위하는 데 필요한 전기, 가스, 상 하수도, 전화, 통신 등의 공공시설물을 지하매설물 형태로 설치하여 시민들의 일상생활 및 사회 경제활동의 편의성을 제공하고 안전하고 안락한 도시환경을 유지시키며, 효율적인 도시운영이 가능케 하는 공공시설물이다. 그러나, 최근 들어 여의도 공동구 화재(2000년), 여수 공동구 침수(2003년), 구리시 전력구 화재(2006)등과 같이 공동구 및 전력구에서의 화재, 혹은 침수 등에 의해 재해가 발생하여 이로 인한 국가적 경제적, 사회적 손실이 늘어가고 있다. 하지만 국내에서는 공동구내 설비를 화재 및 재해로부터 보호하기 위한 법적 장치가 미비한 실정이며, 현재 운영되고 있는 공동구의 관리 또한 시설관리공단이나 위탁관리기관 등으로 분산되어 있어 일정한 시설물 설치기준이나 유지관리제도가 정립되지 못하여 효율적인 운영이 이루어지지 않다고 판단된다. 본 연구에서는 근래 국내에서 발생한 공동구 재해사례와 공동구 관련 법규를 검토 분석하였으며, 다양화되어 가는 공동구 수용시설 조건에 부합하고 체계적이고 효율적인 공동구의 시공을 위한 공동구 건설기준 제정방안을 도출하였다.

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The Effects of Amino Acid Levels with Protein the Diet on Broiler Performance (사료의 단백질 수준에 따른 아미노산 첨가수준이 브로일러의 생산성에 미치는 영향)

  • 정방균;곽종형
    • Korean Journal of Poultry Science
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    • v.18 no.1
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    • pp.43-55
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    • 1991
  • This experiment was conducted to study the sparing effects of animal proteins on weight gain, nutrients utilizability and economic analysis of broiler. Experiment diet contained different ratio of animal and plant protein and were supplemented different levels of methionine and lysine for the respective protein levels. A total of 264 broiler chicks were fed four diets of control, $T_1$, $T_2$ and $T_3$ for 42 days from April 7, 1990 to May 19, 1990. Dietary protein levels of control, $T_1$, $T_2$ and $T_3$ for starter and finisher were 20~18, 18~16, 18~16 and 22~20%, respectively. Methionine and lysine levels of control, $T_1$, $T_2$ and $T_3$ were 0.4~1.1, 0.44~1.21, 0.48~1.32 and 0.48~1.32% for starter diet, respectively, and were 0.32~0.90. 0.35~0.99, 0.38~1.08 and 0.38~1.08% for finisher diet, respectively. The results obtained were summarized as follows. 1. The birds fed control diet gained most for overall period. $T_3$ treatment which was high in protein, methionine and lysine levels gained most for finisher period. 2 The birds fed control diet consumed most feed, and the birds fed T$_3$ diet consumed least feed. for overall period. Feed conversion during 1~4 weeks was better in $T_1$ (1.51) and $T_2$ (1.53) than in control (1.61) and $T_3$ (1.63) . During 4~6weeks, feed conversion was better in $T_3$ (1.37) and control(1.58) than T, (2.05) and T, (2.16) (P<0.01) 3. Dry matter, crude fiber and NFE utilizability were increased for 1~4 weeks and decreased for 4~6 weeks as methionine and lysine levels increased and crude protein utilizability tended to be increased as protein levels increased. 4. Abdominal fat content was lowest in bird fed control diet and was high in birds fed low protein diet Carcass percentage was highest at control and the abdominal fat content was higher in bird fed lower protein diet than bird fed other protein diets(P<0.05). 5. Feed cost per kg weight gain was lowest at $T_3$ which contained more soybean oil meal than other feeds and next was control. According to the results of this experiment, it was revealed that optimum protein, methionine and lysine levels for starter and finisher broiler diet were 20~18, 0.4~0.32 and 1.1~0.9%, respectively.

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Comparison of Egg Production among Crossbreds with Resistance to Fowl Typhoid in Egg Type Chickens (산란계에 있어서 가금티푸스 저항성 계통의 산란성 비교 연구)

  • 오봉국;한성욱;김기석;한경택
    • Korean Journal of Poultry Science
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    • v.30 no.1
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    • pp.1-10
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    • 2003
  • The objects of this study were to develop a new strain which has resistance to fowl typhoid, high performance in laying, and producing brown shell eggs favored by domestic consumers. Several White Leghorn (WL) breeds known as possessing genetic resistance to fowl typhoid and several brown shell egg breeds such as susceptible to the disease were used to produce the controlled strains with cross mating between the strains within the breeds and the experimental strains with crossbreeding between the breeds. The crossbred strains were Chungnong(CN) 21, 22, 23, 24, 25 and 26. The controlled strains were ISA brown CC, Hyline brown CC and Lohman brown CC. The survival rates were 99.95% for chicks of age 0∼l7 weeks and 91% for adult chickens of age 18∼72 weeks. There was no difference in survival rate between the crossbred and the controlled strains. The means of age of lst egg laying were 147 to 148 days and no difference was observed between the crossbred and the controlled. The egg Production rates of a9e 18∼72 weeks were 83.76% far the crossbred strains and 77.82% for the controlled strains, which is significantly higher in the crossbred than controlled strains by 6%. The numbers of the hen housed egg Production of age 18∼72 weeks were 292.33 eggs for the crossbred strains and 271.31 eggs fur the controlled strains. The difference of 21 eggs more produced by the crossbred than by the controlled was statistically significant. The mean egg weights of age 18∼72 weeks were 64.32g for the crossbred strains and 60.73g for the controlled strains, and the difference of 3.59g was statistically significant. The feed conversion rates during the age of 18 to 72 weeks were 2.297 for the crossbred strains and 2.454 for the controlled strains. The crossbreds were reduced feed consumption by 157g for 1kg of egg Production which was statistically significant. Haugh unit(H$.$u) at the age of 72 weeks were 82.20 for the crossbred strains and 77.82 for the controlled strains. The crossbred strains were superior quality by 4.38 H$.$u. There were no significant differences in the yolk color and the eggshell thickness between the crossbred strains and the controlled strains. The eggshell color of the crossbred strains was light brown which is the medium color of the white eggshell strains and the brown eggshell strains. The body weights at the ages of 8, 12, 18, 42 and 71 weeks were not different between the crossbred strains and the controlled strains.