• Title/Summary/Keyword: 구로정

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Antibacterial and Antibiofilm Activities of Leaf Extracts of Stewartia koreana against Porphyromonas gingivalis (Porphyromonas gingivalis에 대한 노각나무 잎 추출물의 항균활성 및 생물막 형성 억제 효과)

  • Kim, Hye Soo;Park, Min Jeong;Kim, Soo Jeong;Kim, Bu Kyung;Park, JunHo;Kim, DaeHyun;Cho, Soo Jeong
    • Journal of Life Science
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    • v.31 no.3
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    • pp.330-337
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    • 2021
  • This study was conducted to investigate the potential of Stewartia koreana as oral healthcare materials. The antibacterial activity of ethanol extracts from leaves and branches of S. koreana against oral bacteria was confirmed. The leaf and branch extracts (1 mg/disc) showed antibacterial activity against P. gingivalis only among several tested oral bacteria. The leaf extracts showed higher antibacterial activity, with values similar to those of chlorhexidine, which was used as a positive control. The MIC of the leaf extract against P. gingivalis was 0.4 mg/ml and showed bacteriostatic action. The inhibitory effects of the extract on biofilm formation and on gene expression related to biofilm formation by P. gingivalis were determined by biofilm biomass staining, scanning electron microscopy (SEM), and qRT-PCR analysis. The biofilm production rate and cell growth of P. gingivalis in the cultures treated with 0.2-2.0 mg/ml of S. koreana leaf extracts were significantly decreased in a concentration-dependent manner. The inhibitory effect on the formation of P. gingivalis biofilms at concentrations of 1 mg/ml was confirmed by SEM. The qRT-PCR analysis showed concentration-dependent suppression of the fimA and fimB gene expression associated with fimbriae formation in the cultures treated with 0.2-2.0 mg/ml S. koreana leaf extract. These results support the conclusion that S. koreana leaf extracts can be used as oral healthcare materials derived from natural materials, as demonstrated by the antibacterial action and inhibition of biofilm formation of P. gingivalis.

Growth and Fruiting Characteristics, and Nut Qualities of Castanea crenata by Low-Concentrated Liquid Fertilizer (저농도 액비처리에 따른 밤나무 생장 및 결실특성과 과실품질)

  • Lee, Uk;Hwang, Suk-In;Kim, Mahn-Jo;Kim, Ji-Hye
    • Journal of Korean Society of Forest Science
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    • v.100 no.3
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    • pp.432-440
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    • 2011
  • The objective of this study was to investigate growth and fruiting characteristics (e.g., nut qualities) of chestnut (Castanea cerenata) after applying various fertilizer treatments at the cultivation site in Suncheon. Fertilizer treatments were designed as follow: liquid fertilizer, chemical fertilizer, organic fertilizer, and control. Both liquid and chemical fertilizer treatments provided the best growth in height and basal diameter. In addition, these two treatments were very effective for crown width of the trees between both east-west and north-south orientation. The liquid fertilizer treatment was effective on total length of the fruiting branch and length of the bearing to terminate part. Both liquid and chemical fertilizer treatments produced the longest length of basal to bearing part compared to the other two treatments. The liquid fertilizer treatment showed the most thickened basal diameter of the fruiting branch and the greatest diameter of above and below the bearing burr part. Elongation Index of the fruiting branch (EI) was the highest with liquid fertilizer treatment and the remaining four indices (Production Index of fruiting branch, PI; Ratio of Diameter between below and above bearing burr part, RD; Growth Index of fruiting branch diameter, GI; Thickness Index of fruiting branch or dormant branch, TI) were the highest with the chemical fertilizer treatment. Total number of produced branch per fruiting mother branch and number of small and weak branches per fruiting mother branch were highest on the control and liquid fertilizer treatment; however, all treatments produced similar numbers. The chemical and organic fertilizer treatments produced a high number of fruiting branches per fruiting mother branch, while organic and liquid fertilizer treatments produced a high number of burr per fruiting branch. The rate of commercializing on the basis of nut weight and quantity was higher on control (87.5%) than chemical fertilizer treatment (84.6%); however, the rate was even lower on liquid fertilizer treatment (84.3%) and organic fertilizer treatment (82.7%). The liquid fertilizer treatment showed the highest average of nut weight, while chemical fertilizer treatment showed the highest average number of fruiting burr. There was no significant difference in average number of normal nuts per burr among treatments. The yield per tree was high on chemical (8.2 kg) and liquid (8.0 kg) fertilizer treatments, but there was no significant difference among treatments. In the rate of nut grade on the basis of nut weight and quantity, the liquid fertilizer treatment, 43.5% and 34.3% more than large nut respectively, produced higher value chestnuts compared to other treatments.

Effects of Gypsum on Dry Matter Yield and Chemical Composition of Alfalfa in Reclaimed Tidal Land with Soil Dressing (객토 간척지에서 석고처리가 알팔파 건물수량 및 사료성분에 미치는 영향)

  • Kim, Ji Yung;Jo, Hyun Wook;Lee, Bae Hun;Jo, Mu Hwan;Kim, Byong Wan;Sung, Kyung Il
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.41 no.4
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    • pp.223-233
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    • 2021
  • The objective of this study was to investigate the effect of gypsum on the dry matter yield and the chemical composition of alfalfa in reclaimed tideland with soil dressing. The experimental site was Sukmoon reclaimed tideland. The tideland was reclaimed approximately 17 to 33 years ago and the 70 cm of soil was top-dressed. The soil that covers the reclaimed tideland brought from the island did not treat di-salinized. Treatments were consisted of three groups; control group where no gypsum (G0) was applied and two experimental groups where 2 ton/ha (G2) and 4 ton/ha (G4) of gypsum were applied, respectively. The first harvest was conducted when the alfalfa reached early flowering (open the flower 10%), and after that subsequent harvest was conducted at approximately 35 days intervals. The dry matter yield of the alfalfa showed that G2 was significantly higher in the first year than G0 and G4, and G2 tended to be higher in the second year than G0 and G4, although there were no significant differences between treatments. The reason for the high dry matter yield in G2 was that the soil pH and EC of the soil were at marginal and ideal levels and the coverage and alfalfa botanical composition were also high. In both years, there were no differences in the crude protein, neutral detergent fiber and acid detergent fiber contents and relative feed value between gypsum treatments. Meanwhile, the results in the first and second years showed that the alfalfa dry matter yield were negatively affected by droughts stress in spring and concentrated precipitation in summer. Therefore, this study suggests gypsum treatment in reclaimed tidal land could increase the dry matter yield of alfalfa, and 2 ton/ha of gypsum was the optimum rate.

Characteristics of Leuconostoc spp. isolated from radish kimchi and its immune enhancement effect (무김치에서 분리한 Leuconostoc 속의 특성과 면역증강 효과)

  • Seoyeon Kwak;Seongeui Yoo;Jieon Park;Woosoo Jeong;Hee-Min Gwon;Soo-Hwan Yeo;So-Young Kim
    • Food Science and Preservation
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    • v.30 no.6
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    • pp.1082-1094
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    • 2023
  • The purpose of this study was to examine the characteristics of Leuconostoc spp. isolated from radish kimchi and to investigate the potential for the use of functional ingredients by evaluating enzymatic characteristics, safety, and immune-enhancing effects among the isolates, including Lactobacillus rhamnosus ATCC53103 (LGG) as a control strain. All test strains exhibited β-glucosidase enzyme activity that releases β-1,4 sugar chain bonds. In addition, as a result of antibiotic resistance assay among the isolates, MIC values on 8 antibiotics were below compared to the EFSA standard, and hemolytic experiments confirmed that all showed gamma hemolysis without hemolytic ability. As a result of the antibacterial activity experiment, the Leu. mesenteroides K2-4 strain showed a higher activity than LGG against Bacillus cereus and Staphylococcus aureus. Additionally, the activity of the NF-kB/AP-1 transcription factor increased when the isolates were treated in macrophage RAW cells. These results were related to increasing the high mRNA expression levels on TNF-α and IL-6 by Leu. mesenteroides K2-4 strain to be treated at low concentration. Consequently, we suggest that it will be useful as a candidate for functional food ingredients.

A Study on Determination of Consumptive Use Needed in the Vegetable Plots for the Prevention of Drought Damage (고등채소의 한해를 방지하기 위한 포장 용수량 결정에 관한연구)

  • 최예환
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.15 no.2
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    • pp.2949-2967
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    • 1973
  • The purpose of this study is to find out and determine the minimum consumptive use of water for Korean cabbage and turmp, so that the minimum water requirement can be secured always for a stable cultivation of these vegetables regardless of weather conditions. The experiment was conducted in two periods; first one from May to July and second one from August to October, each experiment with two varieties of cabbage and two varieties of radish with 2 replicants and 15 treatments. The results found from the above are briefly as follows: 1. Since the mean soil moisture equivalent 64 days after the treatment was 28.5% and the soil moisture content at the time was 2.67% which is far less than that of the wilting point, the crop seemed to be extremely caused by a drought. 2. The rate of 51 days after the seeding, soil moisture content of plot No.2 where irrigation has been continuous was the highest or 21.3%, whereas the plot No.14 without irrigations was 11.2% and the lowest. Therefore, the soil moisture content for the minimum qrowth seemed to be 20%. 3. The consumptive coefficient of Blaney and Criddle on cabbage in two periods were K=1.14 and 0.97 respectively, and on radish in two periods were K=1.06 and 0.86 respectively, thus, cabbage was higher than radish. The consumptive coefficient in the first experiment (May-July) was 0.17 to 0.20 higher than the 2nd experiment(August-October). 4. Nomally, cabbage and radish germinate within one week, however, the germination ot these crops which were treated with a suspended water supply from the beginning took two full weeks. 5. When it elapsed 30 days after seeding, the conditions in plot 1,2 and 3 were fairly good however, the crops in the plops other than these showed a withering and the leaves were withered and changed into high green due to an extrem drought. Though it was about same at the beginning, the drought damage on cabbage was worse than that on radish period, and the reasos for this appears in the latter that the roots are grown too deep. 6. The cabbage showed a high affinity between treated plots and varieties. Consequently, it can be said that cabbage is very suseptive to drought damage, and the yield showed a difference of 35% to 56% depending on the selection oe varieties. 7. The radish also showed a high affinity between the treated plots, however, almost us affinity existed between varieties. Therfore, the yield of radish largely depends on the extent of drought, and the selection of variety does not affect at all. 8. The normal consumptive use on cabbage is $0.62{\ell}/sec$, while that on radish is $0.64{\ell}/sec$, and the minimum optimum water requirement that was obtained in this study is $4,000cc/day/m^3$ or $0.462{\ell}/sec/ha$.

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Studies on the Productivity of Individual Leaf Blade of Paddy Rice (수도의엽신별 생육효과에 관한 연구)

  • Dong-Sam Cho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.18
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    • pp.1-27
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    • 1975
  • Experiment I: A field experiment was conducted in an attempt to find the effect of top-dressing at heading time in different levels of nitrogen application and of different positioned leaf blades formed by the treatment of leaf defoliation at heading time on the ripening and the yield of rice. The results obtained are as follows: 1. Average number of ears per hill and average number of grains per ear in different levels of nitrogen application were increased as the amount of nitrogen applied was increased. while the rate of ripened grains the yield of rough rice and the weight of 1, 000 kernels of brown rice were decreased respectively as the amount of nitrogen applied was increased. 2. The rate of ripened grains and the weight of 1.000 kernels of brown rice in different levels of nitrogen, top-dressing at heading time were larger than those in control and increased. The yield of rough rice although statistically significant differences were not recognized, were numerically increased. 3. The rate of ripened grains, the yield of rough rice, the weight of 1, 000 kernels of brown rice and the rate of hulling in different treatments of leaf defoliation were remarkably decreased as the degree of leaf-defoliation became larger. 4. The rate of ripened grains, the yield of rough rice, the weight of 1, 000 kernels of brown rice and the rate of hulling in different combinations of number of remained leaves positioned differently, formed the order of $L_1(flag leaf)>L_2>L_3>L_4$ when only one leaf blade was remained, and were increased as the positions of leaves were higher when two leaf blades. were, remained. 5. In case of decrease in the number of leaf blades positioned differently, by the treatment of leaf. defoliation, rate of ripened grains, the yield of rough rice, the weight of 1, 000 kernels of brown rice and the rate of hulling were increased as the area of remained leaves became larger and the nitrogen content of a leaf blade was increased. 6. There was a tendency that the increase in the amount of fertilizer application made the rate of ripened grains and the weight of 1, 000 kernels of brown rice reduced in any number of remained leaf blades, but the application of top-dressing at heading. time resulted in the reverse tendency. The yield of rough rice showed a tendency to be increased as the amount of basal dressing and top-dressing increased and for the application of top-dressing at heading time, the yield of rough rice was less at the smaller number of those. 7. The productivity effect of the rate of ripened grains and the yield of brown rice covered by leaf blades was more than 50 per cent and that of the. weight of 1, 000 kernels of brown rice was not more than 1.0 percent. As the amount of nitrogen application increased the. effect of leaf blades on the rate of ripened. grains and the weight of 1, 000 kernels of brown rice was increased. The effect of leaf blades on the weight of brown rice was increased as the amount of basal dressing-application, but the effect was decreased as the amount of top-dressing at heading time increased, 8. The productivity effects of different positioned leaf blades on the rate of ripened grains, the yield of rough rice and the weight of 1, 000 kernels of brown rice were in order of $L_1(flag leaf)>L_2>L_3>L_4$ the productivity effects of $L_1$ and $L_2$ had a tendency to be increased as the amount of nitrogen applied was increased. Experiment II: A field experiment was done in order to disclose the effect of the time of nitrogen application on yield component and the effect of different positioned leaves formed by leaf defoliation at heading time on the rate of ripened grains and the yield of rice. The results obtained are as follows: 1. Average number of ears per hill was increased in the treatment of nitrogen application from basal dressing to 22 days before heading and in the treatment of application distributed weekly. Number of grains was increased in the treatment of nitrogen application from 36 days to 15 days before heading. The rate of ripened grains was, lower in the treatment of nitrogen application from top-dressing to 15 days before heading than in that of non-application, was higher in the treatment of nitrogen application within 8 days before heading, and was the lowest in that of application 29 days before heading. The yield of rough rice was the highest in the treatment of nitrogen application from 29 days to 22 days before heading. The weight of 1, 000 kernels of brown rice was a little high in the treatment of application from 29 days to 8 days before heading. 2. The rate of ripened grains the yield of rough rice, the weight of 1, 000 kernels of brown rice and the rate of hulling in different treatments of leaf defoliation were remarkably decreased as the degree of leaf defoliation got larger and there were highly significant differences among treatments. There was also a recognized interaction between the time of nitrogen application and leaf defoliation. 3. In relation to the rate of ripened grains, the weight of 1. 000 kernels of brown rice and the rate of hulling in different numbers of remained leaves positioned differently and their combinations, the yield components were in order of $L_1(flag leaf)>L_2>L_3>L_4$ when only one leaf was remained, which indicated that the components were increased as the leaf position got higher. When two laves were remained, the rate of ripened grains, the yield of rough rice and rate of hulling were high in case of the combinations of upper positioned leaves, and the increase in the weight of 1, 000 kernels of brown rice appeared to be affected most]y by flag leaf. When three leaf blades were remained similarly the components were increased with the combination of upper positioned leaf blades. 4. In case of decreased different positioned leaf blades by treatment of leaf defoliation, there was a significant positive regression between the leaf area, the dry matter weight of leaf blades and the nitrogen contents of leaf blades, and rate of ripened grains and the yield of rough rice, but there was no constant tendency between the former components and the weight of 1. 000 kernels of brown rice. 5. The closer the time of fertilizer application to heading time, the more the rate of ripened grains and the weight of 1, 000 kernels was decreased by defoliation, and the less were the remained leaf blades, the more remarkable was the tendency. The rate of ripened grains and the weight of 1. 000 kernels was increased by the top-dressing after heading time as the number of remained leaf blades. When the number of remained leaf blades was small the yield of rough rice was increased as the time of fertilizer application was closer to heading time. 6. Discussing the productivity effects of different organs in different times of nitrogen application, the productivity effect of a leaf blade on the rate of ripened grains was higher as the time of nitrogen application got later, and in the treatment of non-fertilization the productivity effect of a leaf blade and that of culm were the same. In the productivity effect on the yield of brown rice, the effect of culm covered more than 50 percent independently on the time of nitrogen application, and the tendency was larger in the treatment of non-fertilizer. The productivity effect of culm on the weight of 1. 000 kernels of brown rice was more than 90 percent, and the productivity effect of a leaf blade was increased as the time of application got later. 7. The productivity effect of a leaf blade in different positions on the rate of ripened grains, the yield of rough rice and the weight of 1, 000 kernels of brown rice had a tendency to be increased as the time of application got later and as the position of leaf blades got higher. In the treatment of weekly application through the entire growing period, the rate of ripened grains and the yield of rough rice were affected by flag leaf and the second leaf at the same level, the but the weight of 1, 000 kernels of brown rice was affected by flag leaf with more than 60 percent of the yield of total leaves.

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