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Investigation of postnatal stem cells from canine dental tissue and bone marrow (성견 치계줄기세포 및 골수줄기세포 특성에 관한 연구)

  • Jhin, Min-Ju;Kim, Young-Sung;Kim, Su-Hwan;Kim, Kyoung-Hwa;Lee, Chul-Woo;Koo, Ki-Tae;Kim, Tae-Il;Seol, Yang-Jo;Ku, Young;Rhyu, In-Chul;Chung, Chong-Pyoung;Lee, Yong-Moo
    • Journal of Periodontal and Implant Science
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    • v.39 no.2
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    • pp.119-128
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    • 2009
  • Purpose: The aim of this study was to evaluate the stemness of cells from canine dental tissues and bone marrow. Methods: Canine periodontal ligament stem cells (PDLSC), alveolar bone stem cells (ABSC) and bone marrow stem cells(BMSC) were isolated and cultured. Cell differentiations (osteogenic, adipogenic and chondrogenic) and surface antigens (CD146, STRO-1, CD44, CD90, CD45, CD34) were evaluated in vitro. The cells were transplanted into the subcutaneous space of nude mice to assess capacity for ectopic bone formation at 8 weeks after implantation. Results: PDLSC, ABSC and BMSC differentiated into osteoblasts, adipocytes and chondrocytes under defined condition. The cells expressed the mesenchymal stem cell markers differently. When transplanted into athymic nude mice, these three kinds of cells with hydroxyapatite /${\beta}$- tricalcium phosphate (HA/TCP) carrier showed ectopic bone formation. Conclusions: This study demonstrated that canine dental stem cells have stemness like bone marrow stem cells. Transplantation of these cells might be used as a therapeutic approach for dental stem cell-mediated periodontal tissue regeneration.

Comparison of high molecular coating type dentine hypersensitivity desensitizers (고분자 기반 코팅형 상아질 지각 과민 처치제의 효과에 대한 비교 연구)

  • Roh, Young-Hoon;Lee, Joo-Yeon;Park, Hyun-Jung;Choi, Cha-Nam;Park, Yoon-Jeong;Koo, Ki-Tae;Kim, Tae-Il;Seol, Yang-Jo;Lee, Yong-Moo;Ku, Young;Rhyu, In-Chul;Chung, Chong-Pyoung
    • The Journal of the Korean dental association
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    • v.46 no.11
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    • pp.683-688
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    • 2008
  • Purpose : The aim of this study was to evaluate the clinical efficacy of two commercially available desensitizing agents over 3 weeks on patients with dentin hypersensitivity. Materials and Methods : An oxalate-based solution, Sensblok (NIBEC Inc. Seoul, Korea) & Superseal (Pheonix Inc. Michigan, USA), and a distilled water placebo were compared in a clinical setting. Seven volunteers exhibiting three or more teeth that were sensitive to tactile & air were enrolled in the study (35 teeth total). A visual analog scale (VAS) was used for recording each patient s level of sensitivity to tactile & air stimuli. A Florida probe was rubbed across the exposed dentin three times with a constant pressure 20 grams to measure the tactile stimulus. The air stimulus was generated by dental unit air syringe for 1-second blast from 1/2 inch distance. The desensitizing agents were applied according manufacture s guideline. VAS scores were recorded at baseline, 1 minute after treatment, at 1 week, 2weeks, 3weeks. VAS score were analyzed using a repeated measures ANOVA ( =0.05). Results : The Sensblok, Superseal, and distilled water placebo all decreased dentin sensitivity. A statistically significant difference existed between Sensblok and placebo when tactile stimulus was applied (P<0.05), but no significant difference was found among other desensitizing agents and tactile and air stimuli.

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The Diet Composition of the Serotine Bat, Eptesicus serotinus Revealed by Faecal Analysis (문둥이박쥐(Eptesicus serotinus)의 배설물을 이용한 먹이원 분석)

  • Chung, Chul-Un;Han, Sang-Hoon;Cha, Jin-Yeol;Kim, Sung-Chul;Kim, Jeong-Jin;Jeong, Jong-Chul;Lim, Chun-Woo
    • Korean Journal of Environment and Ecology
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    • v.29 no.3
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    • pp.368-373
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    • 2015
  • Faecal analysis was carried out to identify the diet composition of Eptesicus serotinus after collecting the faeces from 25 female bats. Five bats were captured at a bridge where a colony of E. serotinous was found in Andong city, Gyeongbuk, every month from May to September 2014. A total of 100 faeces were collected and the parts of insects obtained from the faeces were examined using a high-resolution microscope (50 ${\times}$ binocular microscope) and then the orders of the insects were identified by the characteristics of the parts of the insect (mouthpart, leg, wing, antenna, elytra, etc.). According to the results of the faecal analysis, 4 orders: Coleoptera, Hymenoptera, Diptera and Hemiptera were used as food resource of E. serotinus. Coleoptera was found in May, July, August, and September. Interestingly, only Coleoptera were found in May and July. Hemiptera, Diptera, and Hymenoptera were found to be used as food resources in September, June, and August.

An Analysis of the Effect of Climate Change on Byeongseong Stream's Hydrologic and Water Quality Responses Using CGCM's Future Climate Information (CGCM 미래기후정보를 이용한 기후변화가 병성천 유역 수문 및 수질반응에 미치는 영향분석)

  • Choi, Dae-Gyu;Kim, Mun-Sung;Kim, Nam-Won;Kim, Sang-Dan
    • Journal of Korea Water Resources Association
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    • v.42 no.11
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    • pp.921-931
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    • 2009
  • For the assessment of climate change impacts for the Byeongseong stream, CGCM 3.1 T63 is selected as future climate information. The projections come from CGCM used to simulate the GHG emission scenario known as A2. Air temperature and precipitation information from the GCM simulations are converted to regional scale data using the statistical downscaling method known as MSPG. Downscaled climate data from GCM are then used as the input data for the SWAT model to generate regional runoff and water quality estimates in the Byeongseong stream. As a result of simple sensitivity analysis, the increase of CO2 concentration leads to increase water yield through reduction of evapotranspiration and increase of soil water. Hydrologic responses to climate change are in phase with precipitation change. Climate change is expected to reduce water yields in the period of 2021-2030. In the period of 2051-2060, stream flow is expected to be reduced in spring season and increased in summer season. While soil losses are also in phase with water yields, nutrient discharges (i.e., total nitrogen) are not always in phase with precipitation change. However, it should be noted that there are a lot of uncertainties in such multiple-step analysis used to convert climate information from GCM-based future climate projections into hydrologic information.

Condensable Gas Separation using Phenol! Alumina Composite Activated Carbon Hollow Fiber Membranes (페놀수지/알루미나 복합 활성탄소중공사막을 이용한 응축성 기체 분리)

  • Shin, Kyung-Yong;Park, You-In;Kim, Beom-Sik;Koo, Kee-Kahb
    • Membrane Journal
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    • v.20 no.4
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    • pp.312-319
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    • 2010
  • Carbon membrane materials have received considerable attention for the gas separation including hydrocarbon mixture of ingredients of the volatile organic compounds(VOCs) because they possess their higher selectivity, permeability, and thermal stability than the polymeric membranes. The use of activated carbon membranes makes it possible to separate continuously the VOCs mixture by the selective adsorption-diffusion mechanism which the condensable components are preferentially adsorbed in to the micropores of the membrane. The activated carbon hollow fiber membranes with uniform adsorptive micropores on the wall of open pores and the surface of the membranes have been fabricated by the carbonization of a thin film of phenolic resin deposited on porous alumina hollow fiber membrane. Oxidation, carbonization, and activation processing variables were controlled under different conditions in order to improve the separation characteristics of the activated carbon membrane. Properties of activated carbon hollow fiber membranes and the characterization of a gas permeation by pyrolysis conditions were studied. As the result, the activated carbon hollow fiber membranes with good separation capabilities by the molecular size mechanism as well as selective adsorption on the pores surface followed by surface diffusion effective in the recovery hydrocarbons have been obtained. Therefore, these activated carbon membranes prepared in this study are shown as promising candidate membrane for separation of VOCs.

Maturity and Spawning of Korean Flounder Glyptocephalus stelleri (Schmidt) in the East Sea of Korea (동해안 기름가자미 Glyptocephalus stelleri (Schmidt)의 성숙과 산란)

  • Cha, Hyung Kee;Kwon, Hyeok Chan;Lee, Sung Il;Yang, Jae Hyeong;Chang, Dae Soo;Chun, Young Yull
    • Korean Journal of Ichthyology
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    • v.20 no.4
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    • pp.263-271
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    • 2008
  • The maturity and spawning of Korean flounder Glyptocephalus stelleri were investigated using samples randomly collected in the East Sea of Korea from May 2005 to September 2008. Gonadosomatic index began to increase in December and reached a maximum between March and May. After spawning it began to decrease from June. The spawning period was March to June, and the main spawning period was April to May. Annual reproductive cycles of this species can be divided into four successive stages; immature stage (July~November), maturing stage (December~February), mature stage (March~April) and spent stage (May~June). The fecundity ranged from 15,146 eggs at 24.1 cm (TL) to 101,491 eggs at 38.1 cm (TL). The relationship between total length (TL) and fecundity (F) can be expressed as $F=0.0004TL^{3.449}$ ($R^2=0.663$), with F increasing with TL. The TL at 50% group maturity ($TL_{50%}$) was estimated to be 25.6 cm.

Survey study of powered toothbrush with guiding device for oral hygiene improvement (유도장치가 부가된 진동회전방식 전동칫솔의 구강건강증진효과에 대한 조사연구)

  • Park, Yun-Soo;Lee, Chul-Woo;Hahm, Byoung-Do;Koo, Ki-Tae;Kim, Tae-Il;Seol, Yang-Jo;Lee, Yong-Moo;Rhyu, In-Chul;Gu, Young;Chung, Chong-Pyoung
    • Journal of Periodontal and Implant Science
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    • v.39 no.4
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    • pp.407-412
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    • 2009
  • Purpose: This study was performed to evaluate the effect of powered toothbrush with a wireless remote display on the subjective and objective oral hygiene improvement. Methods: One hundred and fifteen subjects in healthy or mild gingivitis status between the ages of 20 and 90 were recruited and reviewed for study inclusion criteria. At first visit, 115 pre-screened subjects filled in the questionnaire form which consisted of demographic factors, behavioral factors (smoking, alcohol consumption), toothbrushing habits (brushing time and frequency), self-reported oral health status, and self-satisfaction. Baseline clinical indices (Plaque index, Gingival index) were also recorded by a periodontist. Subjects were instructed how to use powered toothbrush with a wireless remote display, and were provided with it. Thirty days after first visit, 90 subjects returned for the second assessment by self-reported questionnaire form and professional clinical checkup. Statistical analysis was performed using paired t-test for the difference between baseline and second visit data. The relationship among variables was examined with chi-square test and Fisher' s exact test. Results: Significant differences were not found on self-reported satisfaction related with sex, smoking, alcohol consumption (P<0.05). Self-reported tooth brushing habit was improved in the aspect of brushing time and frequency. Significant differences were found on the self-reported oral health status, self-satisfaction, and clinical indices between the baseline and second visit data (P<0.01). Clinical indices were significantly reduced after using powered toothbrush with a wireless remote display (P<0.01). No adverse reactions were reported during the study period. Conclusions: Powered toothbrush with a wireless remote display successfully promoted oral hygiene from the subjective and objective viewpoint after 30 days of home usage.

Effects of Perceptual Motor Program on Visual Motor Integration Skill and Motor Skill of a Child With Attention Deficit Hyperactivity Disorder: Single Subject Research Design (지각운동프로그램이 주의력결핍과잉행동장애아동의 시각운동통합기술과 운동기술에 미치는 영향)

  • Kim, Seung-ju;Gu, Kippeum;Kim, Kyeong-Mi
    • The Journal of Korean Academy of Sensory Integration
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    • v.15 no.1
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    • pp.21-32
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    • 2017
  • Objective : The purpose of this study is to explore the effectiveness of a perceptual motor program in promoting visual motor and motor control outcomes in a child with Attention-Deficit/Hyperactivity Disorder (ADHD) who is enrolled in an elementary school. Methods : The subject is 8-year-old boy who was diagnosed with ADHD but did not receive medication. Research design is a single-subject A-B-A(follow-up). Baseline and follow-up phase were 3 sessions and intervention phase were 8 sessions. After performing 40 minutes of free play on the baseline A and follow-up A, we used the copying subtest of the Korean Development Test of Visual Perception 2 (K-DTVP-2) for evaluating visual motor integration skill and the rope jumping subtest for motor skill. After providing the perceptual motor activity in intervention phase B, copying and ropejumping were performed. Results : The standard scores of the Copying for measuring visual motor integration skill were poor at an average of 4.7 points at baseline phase A, but maintained an average of 9.6 points at intervention phase B and an below average of 7.7 points at the follow-up phase A. In the Rope jumping for the motor skill, it was increased by 4.3 times in the baseline phase A and 5.9 times in the intervention phase B, but slightly decreased by 5 times in the follow-up phase A. Conclusion : This study suggests that perceptual motor program has a positive effect on visual motor and motor function of ADHD children.

Effects of Interactive Metronome Intervention on Behavior Symptoms, Timing, and Motor Function of Children With ADHD (상호작용식 메트로놈 중재가 주의력결핍과잉행동장애의 행동증상, 타이밍, 및 운동기능에 미치는 효과)

  • Gu, Kippeum;Kang, Jewook;Lee, Soomin;Kim, Kyeong-Mi
    • The Journal of Korean Academy of Sensory Integration
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    • v.15 no.2
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    • pp.35-45
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    • 2017
  • Objective : The purpose of this study is to investigate the effect of modified Interactive Metronome (IM) program which is applicable to clinical practice based on the IM protocol on the behavioral symptoms, timing and motor function of children with Attention Deficit Hyperactivity Disorder (ADHD). Method : This study used one-group pretest-posttest research design. 13 ADHD children aged 7-12 years in Busan were participated in this study. The participants were underwent 24 sessions of 30 minutes intervention, 3 times a week. Evaluations were performed before- and after the intervention. Measurements used in this study were Korean-ADHD Rating Scale for behavior symptom, Long Form Assessment (LFA) for the timing, and second version of Bruininks-Oseretsky Test of Motor Proficiency (BOT-2) for the motor function. Results : There was a significant improvement in hyperactivity / impulsivity among the behavioral symptoms, and there was a statistically significant improvement in timing, hand coordination, and body coordination. Conclusion : Modified IM program for clinical application has significant effect on improving behavioral symptoms, timing and motor function of children with ADHD.

Obesity among Secondary School Girls in Ulsan Area (울산지역 중학교 여학생의 비만실태에 관한 연구)

  • 김혜경
    • Journal of the East Asian Society of Dietary Life
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    • v.6 no.1
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    • pp.33-39
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    • 1996
  • The aim of this study is to estimate the prevalence of obesity and factors with it Ulsan secondary school girls. A cross-sectional study involving a sample of 485 students aged 12 to 15 years was obtained form secondary schools. Obesity was determined using body mass index(BMI=wt/Ht$^2$). The findings revealed that the average BMI was 19.3, and 4.5% of girls were either overweight and obese(BMI$\geq$25). Subjects were grouped into three categories based on the BMI, sibling obesity, self-consciousness of obesity, eating patterns such as eating snack after dinner and prejudice in food selection, and food frequency score were significantly associated nth BMI, while nutrition knowledge score was not associated with BMI. The fact that several social, environmental and health factors are associated with obesity means that a multisectorial intervention program must be considered in order to decrease the problem of overweight among secondary girls. Such program should include dietary intervention to modify food habits, nutrition education through mass media, introducing information on prevention and control obesity in school curriculum, and allocating sufficient time in the school schedule for exercise activities.

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