• Korean Journal of Food Science and Technology
• /
• v.49 no.6
• /
• pp.610-616
• /
• 2017

${\gamma}$-Glutamyltranspeptidase (GGT, EC 2.3.2.2) transfers ${\gamma}$-glutamyl moiety from glutamine to amino acids or peptides and hydrolyzes glutamine to glutamate and ammonia. In order to overproduce ${\gamma}$-glutamyltranspeptidase from Bacillus amyloliquefaciens (BAGGT), the encoding gene was cloned and expressed in Bacillus subtilis. The productivity of BAGGT in Bacillus subtilis was improved by 42-fold by using a dual-promoter system that was generated by combining promoters from B. subtilis ${\alpha}$-amylase and BAGGT genes. Through optimization of medium composition by Plackett-Burman design and central composition design, BAGGT was produced at $18.3{\times}10^7U/L$ of culture in the optimized medium. Compared to previously used Luria-Bertani medium, the optimized culture medium (15 g/L molasses, 60 g/L corn steep liquor, 6 g/L yeast extract, 4 g/L NaCl, 6 g/L $K_2HPO_4$, and 2 g/L $KH_2PO_4$), resulted in a 4.3-fold increase in production of BAGGT.

• Current Research on Agriculture and Life Sciences
• /
• v.15
• /
• pp.123-131
• /
• 1997

The alternation of light transmissivity of covering films used in most greenhouses over years become of concern. Recently, durable films over 0.1mm in thickness which having 3~5 years life time were popularly used in many greenhouses. However, the films have been replaced yearly with new ones because of lowering light transmittance. The replacement caused negative environmental impact as well as cost increasement. This study was conducted to find fundamental data for developing of a cleaning machine for covering films of greenhouses.

• Elastomers and Composites
• /
• v.49 no.1
• /
• pp.1-12
• /
• 2014

Blocking of fuel filter in aircraft greatly can affect loss of pilot's life and of the aircraft. The investigation of failures is, thus, of vital importance in preventing incidents in advance. The fuel filter of aircraft plays an important role in filtering various debris from jet fuel. It filters impurities smaller than 10 ${\mu}m$ particles from jet fuel provided with the speed of 1,330 pounds per hour. It must be replaced per 500 h on the basis of operating time. However, even before reaching 500 h, the warning sign lighted on due to blocking of fuel filter. Recently, these similar defects have happened repeatedly. Therefore, in this study, the cause of blocking fuel filter in aircraft was investigated using various analytical techniques such as FT-IR microscopy, FE-SEM/EDS, and total sulfur determinator. Consequently, the blocking material of fuel filter was identified to an accelerator from the integral fuel tank sealant. And a mechanism for the formation of the blocking material of fuel filter by an accelerator blooming phenomenon in fuel tank was suggested.

• Journal of Korean Ophthalmic Optics Society
• /
• v.20 no.3
• /
• pp.293-300
• /
• 2015

Purpose: To evaluate blue light hazards of LED lightings in an optical store with blue light radiance used as the quantitative indicators of photobiological hazard. Methods: The spectral radiance of each LED lightings was measured, and blue-light radiance and the corresponding maximum exposure time were calculated. Then each LED lighting was classified according to the risk group from IEC 62471 standard. Results: The yellow LED lightings used in showcases and white LED lightings used on ceilings and logo were classified into risk group RG0. But the white LED lightings used on showcases were classified into risk group RG1. The blue light radiances of white LED lightings used in showcases are dozens of times larger than that of fluorescent lamp. Conclusions: Using the value of the blue light radiance could quantitatively express the blue light hazard to various lightings. It was confirmed that white LED lightings for the showcases had high blue light hazards because of their high luminance and color temperature. Therefore, when replacing lightings in optical shop it is necessary to select the appropriate brightness and color temperature for eye health in the long term.

• Journal of the Institute of Electronics and Information Engineers
• /
• v.50 no.9
• /
• pp.68-73
• /
• 2013

In this paper, we proposed a readout circuit of pulse waveform and rate for a U-health system to monitor health condition. For long-time operation without replacing or charging a battery, either pulse waveform or pulse rate is selected as the output data of the proposed readout circuit according to health condition of a user. The proposed readout circuit consists of a simple digital logic discriminator and a dual-mode ADC which operates in the ADC mode or in the count mode. Firstly, the readout circuit counts pulse rate for 4 seconds in the count mode using the dual-mode ADC. Health condition is examined after the counted pulse rate is accumulated for 1 minute in the discriminator. If the pulse rate is out of the preset normal range, the dual-mode ADC operates in the ADC mode where pulse waveform is converted into 10-bit digital data with the sampling frequency of 1 kHz. These data are stored in a buffer and transmitted by 620 kbps to an external monitor through a RF transmitter. The data transmission period of the RF transmitter depends on the operation mode. It is generally 1 minute in the normal situation or 1 ms in the emergency situation. The proposed readout circuit was designed with $0.11{\mu}m$ process technology. The chip area is $460{\times}800{\mu}m^2$. According to measurement, the power consumption is $161.8{\mu}W$ in the count mode and $507.3{\mu}W$ in the ADC mode with the operating voltage of 1 V.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.28 no.3
• /
• pp.363-368
• /
• 2001

It is well known that numerous factors influence the light output of curing units, but many dentists are un aware that the output of their curing lights are inadequate. This study was conducted to evaluate the light in tensity of visible-light curing units in some private dental clinics and hospital dental clinics. In order to determine the maximum light intensity of the curing units, lamps, filters and fiber optic bundles, they were replaced with new ones and light intensity was remeasured. Light intensity was measured by employing a digital radiometer (EFOS model #8000, USA). Light intensity ranged in $29\sim866mW/cm^2$ (below $150mW/cm^2$ ; 17.8%, $150\sim300mW/cm^2$ : 46.6%, above $300mW/cm^2$ ; 35.6%). The replacement of the components increased the light intensity, with maximum increases of 94.8% for lamps, 82.3% for filters, 200.8% for fiber optics and 361.5% for all three parts. According to the manufacturer of radiometer, curing light is considered as unsuitable for use with a reading of above $300mW/cm^2$ by the radiometer. Applying these criteria to the present study, 64.4% of the curing units required repair or replacement. The results of this study indicated that the light intensities of the curing units used in dental practice were lower than optimum level.

• Journal of Korea Multimedia Society
• /
• v.11 no.8
• /
• pp.1082-1092
• /
• 2008

In wireless sensor networks, clustering protocol such as LEACH is an efficient method to increase whole networks lifetime. However, this protocol result in high energy consumption at the cluster head node. Hence, this protocol must changes the cluster formation and cluster head node in each round to prolong the network lifetime. But this method also causes a high amount of energy consumption during the set-up process of cluster formation. In order to improve energy efficiency, in this paper, we propose a new cluster formation algorithm. In this algorithm, we define a intra cluster as the sensor nodes within close proximity of each other. In a intra cluster, a node senses and transmits data at a time on the round-robin basis. In a view of whole network, intra cluster is treated as one node. During the setup phase of a round, intra clusters are formed first and then they are re-clustered(network cluster) by choosing cluster-heads(intra clusters). In the intra cluster with a cluster-head, every member node plays the role of cluster-head on the round-robin basis. Hence, we can lengthen periodic round by a factor of intra cluster size. Also, in the steady-state phase, a node in each intra cluster senses and transmits data to its cluster-head of network cluster on the round-robin basis. As a result of analysis and comparison, our scheme reduces energy consumption of nodes, and improve the efficiency of communications in sensor networks compared with current clustering methods.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.38 no.12
• /
• pp.1223-1231
• /
• 2010

The design is presented for a SATCOM antenna capable of simultaneous multi-band (X/Ku/Ka-Band) communications without replacement of feed horns or change of other parts in the application as a ground satellite terminal for large data transfer. The antenna is the offset configuration and consists of a dual-band(X/Ka-band) feed horn, a single-band(Ku-band) feed horn, a frequency selective surface(FSS) sub-reflector and a parabolic main-reflector. The antenna has a main reflector defining a prime focus and a frequency selective surface sub-reflector defining an image focus. A dual-band feed and a single-band feed are provided at each of the prime focus and image focus. The antenna is designed using 3D EM simulator and the gains measured in X/Ku/Ka-band of the complete antenna assembly is more than 31.6dBi, 36.8dBi, 40.8dBi, and the cross polarization is 21.7dB, 26.6dB, 25.2dB, respectively.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.13 no.1
• /
• pp.39-44
• /
• 2012

The new blind design of sunlighting has been suggested in this study. The material in this blind becomes transparent but the blind has the parabolic edge section having the perpendicular line on a side. The material of this blind is PolyMethly MethAcrylate(PMMA). In this parabolic edge section, the front side of the blind is designed perpendicular to the ground. But the back side is a little tilted to the front side. The rays of reflected sun at the front side can be easily reflected totally by the back side. If the inclination angle in this parabolic edge section at the back side is designed with $15^{\circ}$, it can transmit the rays of sun when the height of the sun is lower than $45^{\circ}$. But it can reflect the rays of sun when the height of the sun is upper than $45^{\circ}$. The suggested design of blind can be applied to the existing blind installation. Although the material in this blind becomes transparent, the rays of sun can be reflected totally at midday. There is also prospect outside of the blind because the material becomes transparent. Several inclination angles in the suggested design have been simulated for the various height of sun. Total reflections have been occurred by the suggested blind design at midday and it can be useful to shut out the sunlight.

• International Journal of Highway Engineering
• /
• v.8 no.2 s.28
• /
• pp.1-12
• /
• 2006

An existing Steel pipe, Cast iron pipe and Concrete pipe is can not escaped from aging, specially Metal tube is causing many problems that the quality of water worse is concerned about many rust and mike efficient use of preservation of water. The use of Glassfiber Reinforced Plastic Pipe(GRP PIPE) should be one of the possible scheme to get over these problems. The GRP PIPE has an excellent resistance power and the life is lasting from 50 to 100 years roughly. It's to be useful as a result of high durability and a good construction work also it is a light weight therefore can be expected to short the time of construction and man power. In this research, to executed the small-scaled model test, in-situ model test using CLSM of in-situ soil and to evaluated the stress - strain of the pipe also try to estimated how useful is. From the model test in laboratory, the vertical and horizontal deformation of the GRP PIPE measured in six instance using 200mm and 300mm in diameters. The value of experimentation, theory, analysis got the same results of the test, but the vertical and horizontal deformation gauged in small and the earth pressure was almost zero using CLSM of in-situ soil..